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Gene information
General information
TC013646 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7101352..7105488
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013646
    iBeetle screen iB_10017

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013646 RA
      MAERKEEAQ DCLENTLNL NRHEEIELQ TLTGVPNSA 
      NKLQSQKYA NVPERGTWA TKLDFILSV VGLAIGLGN 
      VWRFPYLCY KNGGGAFLV PYFITLILA GIPMFFMEL 
      ALGQMMTIG GLGVFKIAP IFKGIGYAA AVMSCWMNI 
      YYIVILAWA IFYFFMSLR SDVPWRTCN NYWNTKYCV 
      NPYDRSSLT CWEQYNPNN TFSKICAIN QHNISVTEL 
      TDPVKEFWE RRALQISEG IEHVGSIRW ELAGTLLLV 
      WIVCYFCIW KGVKWTGKV VYFTALFPY VLLTILLIR 
      GVTLPGAME GIKFYVMPN IAKLKESEV WIDAVTQIF 
      FSYGLGLGT LVALGSYNK FTNNVYKDA LIVCCVNSS 
      TSMFAGFVI FSVVGFMAH EQQKPVAEV AASGPGLAF 
      LAYPSAVLQ LPGSPLWAC LFFFMILLI GLDSQFCTM 
      EGFVTAIID EWPHLLRKR KEVFIAIVC ALSYLVGLS 
      CISEGGMYV FQILDSYAV SGFCLLFLI FFECIAISW 
      AFGVNRFYD GIKEMIGYY PISWWKFCW TITTPVICI 
      GVFIFNIIQ WTPVKYLNY EFPLWAHLF GWFTALTSM 
      LCIPGYMVW LWMVTPGDR ETKIRLLVR IEDDVPTVR 
      AKMQLETSS VAV
    CDS sequence
    • >TC013646 RA
      atggccgag aggaaagaa gaagcgcaa gattgctta 
      gaaaacacc cttaactta aatcgacac gaagaaatc 
      gaattacaa acattaacc ggtgttccg aactcagcg 
      aataagtta cagtcgcag aaatatgcg aatgtaccg 
      gaacgaggg acgtgggct acaaaacta gactttatt 
      ttatcggta gtcgggttg gctataggt ttagggaat 
      gtttggcgg tttccttac ttgtgttac aaaaatggt 
      ggtggtgcc ttccttgta ccttacttt atcacactt 
      atccttgct ggtatccca atgttcttc atggagctg 
      gcactgggc caaatgatg acgatcggc ggcttgggc 
      gttttcaaa atcgcacca attttcaaa ggaatcggt 
      tatgccgcc gcggttatg tcctgctgg atgaacatc 
      tactacatc gtcatccta gcctgggcg attttctat 
      ttcttcatg tcattacga tcagatgtt ccttggcgc 
      acttgtaac aattactgg aacaccaaa tattgtgtt 
      aacccttac gatcgtagt tcactaact tgttgggag 
      cagtacaac cctaataac acctttagc aaaatctgt 
      gctattaac caacacaac atatcagta accgaacta 
      accgaccct gttaaagaa ttttgggaa cgaagggct 
      ctgcaaatt tccgaaggc attgagcac gtgggctca 
      attcggtgg gaattggcc gggactttg cttttagtc 
      tggatcgtt tgctacttc tgtatttgg aaaggcgtc 
      aaatggacc gggaaagtg gtctacttc accgctctt 
      ttcccgtac gtccttctc accatttta ctgatccga 
      ggtgttacc ttgccgggc gccatggaa ggcatcaag 
      ttctacgtg atgcccaac atcgcaaaa ctgaaggag 
      tccgaagtc tggatcgat gcagtcacc caaattttc 
      ttctcttac ggtttaggt ttagggacg ttggtggct 
      ttgggcagt tataacaag ttcaccaac aatgtatat 
      aaggatgct ttaatcgtg tgttgtgtt aactcaagc 
      acgagcatg ttcgccggc tttgtcata ttttcagtg 
      gtgggcttc atggcgcac gaacagcaa aagccggtg 
      gcagaagtc gctgcttca ggacctgga ctggccttc 
      ctggcgtac ccttccgca gttttgcaa ctcccggga 
      tcaccactg tgggcttgc ttgttcttc tttatgatc 
      cttctgata ggtttggac tcgcaattt tgtacgatg 
      gaaggcttc gtcactgcc atcatcgac gagtggccg 
      catcttctc cgcaaaaga aaagaagtc ttcattgcg 
      atcgtctgc gcattgtca tacctcgtc ggattatcg 
      tgcatttcc gagggtggc atgtacgtg ttccaaatt 
      ttggattcg tacgccgtc agcggcttc tgcctcctg 
      ttcttgatt ttcttcgaa tgtatcgcg atttcgtgg 
      gctttcggc gtgaaccga ttctacgac ggaattaag 
      gaaatgatc ggctattac ccgatcagt tggtggaag 
      ttttgttgg accattact acaccagtg atctgcatt 
      ggcgtattt attttcaat ataatccag tggacaccg 
      gtcaagtat ttgaattac gaatttccg ctatgggct 
      catctcttt ggttggttt actgcgctt acttccatg 
      ctatgcata ccgggatac atggtttgg ctctggatg 
      gttactcca ggagataga gaaacgaaa attcggctt 
      cttgtaaga atagaggat gatgtcccc actgttcga 
      gcgaaaatg cagttggaa acatcgtca gtggcagta 
      taa
    mRNA sequence
    • >TC013646 RA
      cgcgtagca ggagtcagt ggtccagag tgcttcaag 
      aagaccact cttcggtgt ttggtgtcg cgtttccgc 
      gtagtgcag tgtttgtga atttacaca ccacgtgga 
      ttacatcgg ttgggattt ggtctgtaa tgagctgac 
      gtttcgcaa agtgtgaga agaaatact gagagaatg 
      gccgagagg aaagaagaa gcgcaagat tgcttagaa 
      aacaccctt aacttaaat cgacacgaa gaaatcgaa 
      ttacaaaca ttaaccggt gttccgaac tcagcgaat 
      aagttacag tcgcagaaa tatgcgaat gtaccggaa 
      cgagggacg tgggctaca aaactagac tttatttta 
      tcggtagtc gggttggct ataggttta gggaatgtt 
      tggcggttt ccttacttg tgttacaaa aatggtggt 
      ggtgccttc cttgtacct tactttatc acacttatc 
      cttgctggt atcccaatg ttcttcatg gagctggca 
      ctgggccaa atgatgacg atcggcggc ttgggcgtt 
      ttcaaaatc gcaccaatt ttcaaagga atcggttat 
      gccgccgcg gttatgtcc tgctggatg aacatctac 
      tacatcgtc atcctagcc tgggcgatt ttctatttc 
      ttcatgtca ttacgatca gatgttcct tggcgcact 
      tgtaacaat tactggaac accaaatat tgtgttaac 
      ccttacgat cgtagttca ctaacttgt tgggagcag 
      tacaaccct aataacacc tttagcaaa atctgtgct 
      attaaccaa cacaacata tcagtaacc gaactaacc 
      gaccctgtt aaagaattt tgggaacga agggctctg 
      caaatttcc gaaggcatt gagcacgtg ggctcaatt 
      cggtgggaa ttggccggg actttgctt ttagtctgg 
      atcgtttgc tacttctgt atttggaaa ggcgtcaaa 
      tggaccggg aaagtggtc tacttcacc gctcttttc 
      ccgtacgtc cttctcacc attttactg atccgaggt 
      gttaccttg ccgggcgcc atggaaggc atcaagttc 
      tacgtgatg cccaacatc gcaaaactg aaggagtcc 
      gaagtctgg atcgatgca gtcacccaa attttcttc 
      tcttacggt ttaggttta gggacgttg gtggctttg 
      ggcagttat aacaagttc accaacaat gtatataag 
      gatgcttta atcgtgtgt tgtgttaac tcaagcacg 
      agcatgttc gccggcttt gtcatattt tcagtggtg 
      ggcttcatg gcgcacgaa cagcaaaag ccggtggca 
      gaagtcgct gcttcagga cctggactg gccttcctg 
      gcgtaccct tccgcagtt ttgcaactc ccgggatca 
      ccactgtgg gcttgcttg ttcttcttt atgatcctt 
      ctgataggt ttggactcg caattttgt acgatggaa 
      ggcttcgtc actgccatc atcgacgag tggccgcat 
      cttctccgc aaaagaaaa gaagtcttc attgcgatc 
      gtctgcgca ttgtcatac ctcgtcgga ttatcgtgc 
      atttccgag ggtggcatg tacgtgttc caaattttg 
      gattcgtac gccgtcagc ggcttctgc ctcctgttc 
      ttgattttc ttcgaatgt atcgcgatt tcgtgggct 
      ttcggcgtg aaccgattc tacgacgga attaaggaa 
      atgatcggc tattacccg atcagttgg tggaagttt 
      tgttggacc attactaca ccagtgatc tgcattggc 
      gtatttatt ttcaatata atccagtgg acaccggtc 
      aagtatttg aattacgaa tttccgcta tgggctcat 
      ctctttggt tggtttact gcgcttact tccatgcta 
      tgcataccg ggatacatg gtttggctc tggatggtt 
      actccagga gatagagaa acgaaaatt cggcttctt 
      gtaagaata gaggatgat gtccccact gttcgagcg 
      aaaatgcag ttggaaaca tcgtcagtg gcagtataa 
      tttttttac cgaactttc agacgtttt tttaccgtg 
      tcacatctc gtcttccac tcaaatttt cattagtgt 
      tcagacagc tttgtaatt aagcaacta gcatgtttt 
      tatctaatt gtactgttg tatttgtaa gtttgtata 
      cttcataca attagtatg aactttatc gagagtacc 
      gcgtacaaa attcttgta caataactt taattacgt 
      catctgatg tttaaatgt taaagtaaa ttgggatta 
      agtccctta ttatacaat tagcttcat attgtcatt 
      gtttaattt attaacttt ttaatataa gagtaggcc 
      ctaaggaaa tgtagtgtt taacgcata tttaacttg 
      actaataat gtacaataa tatttgttg taaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY