Status
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Gene information
General information
TC013651 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6956408..6959433
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013651
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10014

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013651 RA
      MDNEEERPA DVEGLAVVE GPVDIEGPV DVEGPADAE 
      GPAHVEGPA HVEGPADVE GPADAEGPA HVEGPADVE 
      GPADAEGPA HVEGPANVE GPADVGLEE PPMLDLAAA 
      FENLKIEGS FLSSPLEES VALMTEEVA SSTSATDCK 
      VVSKAEETG SVTDMTESK TTSDESFKT AGDMVEETT 
      TSSEVEETS NTAKAEDVV PGSDPSIRV GNVIEEATT 
      SELNTTLTE GAEIVAKTD SSIRVGSMI EEATTSGGD 
      IKTEEMVPI LPSNSGVSV VAQATASTA NMGDFEDTE 
      KMVSNTGRS KSADSMVVE ATDSTANIA ELDEMLSAI 
      LPSRSIDSM VVQTTASTA NVSDLRDFL HRSREHANA 
      STQKFLAVP PSPGISDYD LYQHLLKSS PTTSNQQLP 
      KLIKIRPDY VITQPIKIK TELKENGSG NNELDEAYK 
      KIANLSDEN KKYVNDLKE LQNKCESTL NNFSLLKES 
      HSKLENELQ LVKEDNVKL SESLRQYDE VMNELCKKN 
      KTMREMYDK EFEKRTRLE AEIKNLTLE GFNGANEMA 
      ENRFKAKIQ EVTNQNSQL KEILKVLRS QLNIEHDKE 
      NKAAAAN
    CDS sequence
    • >TC013651 RA
      atggataac gaggaagaa aggcctgct gatgtcgaa 
      gggcttgct gttgtcgaa ggacctgtt gatatcgaa 
      ggacctgtt gatgtcgaa gggcctgct gatgccgaa 
      ggacctgct catgtcgaa gggcctgct catgtcgaa 
      gggcctgct gatgtcgaa gggcctgct gatgccgaa 
      ggacctgct catgtcgaa gggcctgct gatgtcgaa 
      gggcctgct gatgccgaa ggacctgct catgtcgaa 
      gggcctgct aatgttgaa gggcctgct gatgtcggc 
      cttgaagag cctccgatg cttgatttg gcggctgcc 
      ttcgagaac ctgaagatt gaagggagt ttcctaagc 
      agtccactt gaagagtct gtagctttg atgaccgaa 
      gaagtggcc tcttctaca tctgctacc gactgtaaa 
      gttgtttct aaagcagaa gagacggga agtgttaca 
      gatatgaca gaatccaag accacgtca gatgaatct 
      tttaaaact gctggtgat atggtggag gaaacaact 
      acgtcaagt gaagtagag gaaacgtcg aatactgct 
      aaagcggaa gacgtggta cctggttct gacccttct 
      ataagagtt ggtaatgtg atagaggaa gcaactact 
      tcagagttg aatacaact ttaactgaa ggagcggag 
      attgtagca aaaactgat tcttctata agagttggt 
      agtatgata gaggaagca actacgtca ggaggtgat 
      atcaaaaca gaagagatg gtacctatt ctcccgtcg 
      aatagtggt gttagcgtg gtagctcaa gcgactgcc 
      tcaacagcg aatatgggc gatttcgaa gatacggaa 
      aaaatggtg tccaatact gggcggtcg aaaagtgct 
      gatagtatg gtggtcgaa gccactgac tcaactgca 
      aatattgca gaactagat gaaatgtta tccgcaatt 
      cttccgtct aggagtatt gatagcatg gtggtgcaa 
      acaactgcc tcaacagcg aatgttagc gatctcaga 
      gactttctc catcgaagc agagaacat gccaatgct 
      tccacacag aaattttta gcagtgcct ccgtcaccg 
      ggaatttcg gattatgat ctctatcaa catttgtta 
      aaatcgtct ccaacaaca tcaaaccaa cagttgcct 
      aaattgatt aaaataaga ccagactat gtcataaca 
      caacccata aaaataaag actgaattg aaggagaat 
      ggaagtggt aacaatgaa ttagatgaa gcctacaaa 
      aaaattgca aacttgtca gacgaaaac aaaaaatac 
      gtaaatgat ttgaaagag cttcaaaat aaatgtgag 
      tcaaccttg aacaatttc tcattactc aaagaaagt 
      cactcgaaa cttgaaaac gaacttcaa ttggtgaaa 
      gaggataat gtgaaatta agcgaaagt ttgcgtcaa 
      tatgatgaa gttatgaac gagctttgt aaaaaaaat 
      aaaacaatg cgagagatg tatgacaag gaatttgag 
      aagagaact aggcttgaa gcagaaata aagaatcta 
      acactagag ggatttaac ggggcgaat gaaatggca 
      gaaaataga tttaaggcg aaaatacaa gaagtcact 
      aatcaaaac agtcagttg aaggagata cttaaagtg 
      ttacgttct caactaaac attgagcac gataaggaa 
      aataaggca gcagcggct aattag
    mRNA sequence
    • >TC013651 RA
      aatcaaaac ctgtgtaaa cttgttaaa ggctttagt 
      ttttctgtg taatttagt tgtgcctgt ttttgatat 
      ggagaagca agtcacaat ggataacga ggaagaaag 
      gcctgctga tgtcgaagg gcttgctgt tgtcgaagg 
      acctgttga tatcgaagg acctgttga tgtcgaagg 
      gcctgctga tgccgaagg acctgctca tgtcgaagg 
      gcctgctca tgtcgaagg gcctgctga tgtcgaagg 
      gcctgctga tgccgaagg acctgctca tgtcgaagg 
      gcctgctga tgtcgaagg gcctgctga tgccgaagg 
      acctgctca tgtcgaagg gcctgctaa tgttgaagg 
      gcctgctga tgtcggcct tgaagagcc tccgatgct 
      tgatttggc ggctgcctt cgagaacct gaagattga 
      agggagttt cctaagcag tccacttga agagtctgt 
      agctttgat gaccgaaga agtggcctc ttctacatc 
      tgctaccga ctgtaaagt tgtttctaa agcagaaga 
      gacgggaag tgttacaga tatgacaga atccaagac 
      cacgtcaga tgaatcttt taaaactgc tggtgatat 
      ggtggagga aacaactac gtcaagtga agtagagga 
      aacgtcgaa tactgctaa agcggaaga cgtggtacc 
      tggttctga cccttctat aagagttgg taatgtgat 
      agaggaagc aactacttc agagttgaa tacaacttt 
      aactgaagg agcggagat tgtagcaaa aactgattc 
      ttctataag agttggtag tatgataga ggaagcaac 
      tacgtcagg aggtgatat caaaacaga agagatggt 
      acctattct cccgtcgaa tagtggtgt tagcgtggt 
      agctcaagc gactgcctc aacagcgaa tatgggcga 
      tttcgaaga tacggaaaa aatggtgtc caatactgg 
      gcggtcgaa aagtgctga tagtatggt ggtcgaagc 
      cactgactc aactgcaaa tattgcaga actagatga 
      aatgttatc cgcaattct tccgtctag gagtattga 
      tagcatggt ggtgcaaac aactgcctc aacagcgaa 
      tgttagcga tctcagaga ctttctcca tcgaagcag 
      agaacatgc caatgcttc cacacagaa atttttagc 
      agtgcctcc gtcaccggg aatttcgga ttatgatct 
      ctatcaaca tttgttaaa atcgtctcc aacaacatc 
      aaaccaaca gttgcctaa attgattaa aataagacc 
      agactatgt cataacaca acccataaa aataaagac 
      tgaattgaa ggagaatgg aagtggtaa caatgaatt 
      agatgaagc ctacaaaaa aattgcaaa cttgtcaga 
      cgaaaacaa aaaatacgt aaatgattt gaaagagct 
      tcaaaataa atgtgagtc aaccttgaa caatttctc 
      attactcaa agaaagtca ctcgaaact tgaaaacga 
      acttcaatt ggtgaaaga ggataatgt gaaattaag 
      cgaaagttt gcgtcaata tgatgaagt tatgaacga 
      gctttgtaa aaaaaataa aacaatgcg agagatgta 
      tgacaagga atttgagaa gagaactag gcttgaagc 
      agaaataaa gaatctaac actagaggg atttaacgg 
      ggcgaatga aatggcaga aaatagatt taaggcgaa 
      aatacaaga agtcactaa tcaaaacag tcagttgaa 
      ggagatact taaagtgtt acgttctca actaaacat 
      tgagcacga taaggaaaa taaggcagc agcggctaa 
      ttagaattg aagttagag ttaattgag aaaaactgt 
      agtattgtt tgatatttt tgtttgctt tattttttt 
      tttaattaa taaatattg attttggtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY