Status
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Gene information
General information
TC014157 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6802250..6805919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC014157
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10013

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      larva segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      head appendages structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      legs size shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
      legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

      legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
      posterior terminus not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014157 RA
        MKSKLKLGF CFALCISVT LGFTTDRHS LVVSSTEPP 
KESSSEVAP NKEDVAPIV PKIYEMKVD TNVSNRFAK 
TSVVSKVKN LAKTPQEAT FSVVLPETA YISGFVMEI 
DGKSYKAYV KEKEEAKQI YNEAVGRGQ AAAHVEANA 
RDSNRFTVS LNIEPQKKA VFTLTYEEL LQRQNEQYE 
VVINIHPGQ PVKDLNVEV HIDESRPLK FVKSPPLRT 
GNEISKNDD KTASLAEIK QNNSTSATV KFNPNIERQ 
KQLATGLGT KEENGLAGQ FVVQYDVER DPKGGEVLL 
KDGYFVHFF APSEVEALP KQVIFVLDT SGSMDGNRI 
KQLKEAMNS ILSELKKED VFNIVEFSS IVKVWNVDK 
VQVDYEVGE DPWPLYDSP EAPQKNKTN QVLPPAYKA 
TDENKEKAK KVVEKLNAY GGTDIKSAL EVGLKLVKK 
NKENKEDAH QPIIVFLTD GEPTMGETN TEKITSAIS 
EMNSGETRA PIFSLSFGD GADREFLQK ISLKNLGFA 
RHIYEAADA SLQLQEFYK QISSPLLNN VNFKYVSNV 
TKLTKTVFP IYFRGSEIV VAGVAEDEF LPPSIEGNT 
LRGPIIWSP KVETPVGDL ERLWAYLTI KQILEARDA 
AEKKEPYTK KALDIALKY SFVTPVSSL VVVKPNDTS 
AVDTEDASK TADRPYFLN RNYATPNLR PQLGLAGPS 
TLSFAAAAA GFGGAAPPM PQPAVQEAD LEEDAYMSP 
EMFAPSTTD IPTTTKSQD LYEDIKNKL PWLKDIITV 
NGTITLPNG SYKISTNET QVTSTPQCP KTPINGTAG 
VCTLLPHCP QVFSLLTDA KVFQEFFCP LDTFVGVCC 
PIATDATSA SAAIS
      CDS sequence
      • >TC014157 RA
        atgaaaagt aaactcaaa ctaggattc tgcttcgca 
ttatgcatt tctgtgacc ctcggcttt accacagac 
cggcacagt ttagtggta tcatcaacg gaacctccg 
aaagaatct tcaagtgag gttgccccc aataaggag 
gatgtggcc ccaattgtc ccaaaaatt tacgaaatg 
aaagttgac acaaacgta tcgaatcgc ttcgccaaa 
acttcagtc gtcagtaaa gtgaaaaat ttggcgaaa 
acaccacag gaagccacc ttctcagtc gtgttgccc 
gaaactgcg tacatctcg ggattcgtg atggaaatc 
gacgggaaa agctacaaa gcgtacgtg aaagaaaaa 
gaagaagcg aagcaaatt tacaatgag gctgtcgga 
agaggccaa gcagcggct catgtcgaa gctaacgcc 
agagattcc aacagattc actgtttct ctaaacatc 
gaaccgcaa aagaaagca gttttcact ctcacgtac 
gaagaactg ctccagcgt caaaacgag cagtacgaa 
gttgtaatt aacatccat ccaggccag ccggtcaag 
gacttaaac gtcgaggta cacattgac gaaagcagg 
cctttgaaa ttcgttaag tctccgcct ttgcgcacc 
ggcaacgaa atatccaaa aacgacgac aaaacggcc 
tcgttggcc gaaatcaag caaaataac tcaacttcg 
gcaacggtc aaattcaac ccaaacatc gaacgccag 
aagcaactg gccactggt ttggggacg aaagaggag 
aatgggcta gccggccag ttcgtggtg caatacgac 
gttgaacga gaccccaaa gggggcgag gttctgctc 
aaggatggc tatttcgtc catttcttt gcccccagt 
gaggtggaa gccctaccc aagcaagtt attttcgtc 
ctggatact agtggcagc atggacgga aacagaatc 
aagcagttg aaagaggca atgaacagc attttgagc 
gaattgaag aaggaagat gtgttcaat attgtggaa 
ttcagttcc attgtgaaa gtctggaac gttgacaag 
gtccaagtc gattacgaa gttggcgaa gacccgtgg 
ccgctgtac gacagtcca gaggcccct cagaagaac 
aaaacaaac caggtgttg cccccggcc tacaaagcc 
accgatgaa aataaagaa aaggcgaag aaagttgtg 
gaaaagttg aatgcgtac ggaggcact gatattaaa 
tcggcactg gaggttggt ttgaaactt gtcaagaag 
aacaaagaa aacaaggaa gatgcacat cagccgatt 
attgtgttt ttgaccgac ggtgagcca accatgggc 
gaaaccaac acggagaaa atcacatct gctatttct 
gaaatgaac agtggggaa acgcgcgcc cccattttc 
tcgctttct tttggcgat ggcgctgat cgggaattc 
ttgcaaaaa atatctttg aagaatttg ggtttcgcg 
agacacatt tacgaagct gccgatgcc agtctccag 
ttgcaagaa ttctacaaa caaatttcg tcgccactg 
ttgaacaat gttaacttc aaatacgtc tcaaatgtg 
acaaaactg accaaaacc gtgtttcca atttacttc 
cgaggatcg gaaatcgta gtcgctggc gtggcagag 
gacgaattc ctaccaccc tccatcgag ggaaatact 
ctacgggga ccgatcatc tggagtccg aaagttgag 
acgcctgtt ggcgacctg gagagactt tgggcttat 
ctcacaatt aagcaaata ctcgaagct agggatgcc 
gctgagaag aaggagccc tacaccaaa aaggcgcta 
gatattgct ttgaaatac tcatttgtg accccggtt 
agttcattg gtggtggtc aagccaaac gatacgagt 
gctgtggac acggaggat gcgtcgaaa accgccgac 
aggccgtac tttttaaac agaaattat gcaacacct 
aatttaaga ccccaatta ggtctggca ggcccatca 
actctttcc tttgcagcg gccgcagct gggtttggg 
ggtgccgcc cctccgatg ccccaacct gctgttcaa 
gaagccgat cttgaggaa gatgcatac atgtcacct 
gaaatgttt gctccatct acaactgac ataccaaca 
accacaaaa tcgcaagat ttgtacgaa gacattaag 
aataaattg ccatggctg aaagatatt ataacagtt 
aatggaacg ataacttta cccaacggg agttacaaa 
attagcaca aatgaaaca caagtgacg tcaacccct 
caatgtcca aaaacaccc ataaatggt accgctggc 
gtctgcaca cttttacca cattgcccg caagtgttt 
tcgctctta acagatgcc aaagttttt caggagttt 
ttctgtccc ttggataca tttgttgga gtgtgttgt 
ccaattgca acagatgct acaagcgca agtgcagca 
atttcttaa
      mRNA sequence
      • >TC014157 RA
        ttggtcatc ttatattgt tactgtagt gtgattatt 
tttttaact gttaagtag aataaaaaa atgaaaagt 
aaactcaaa ctaggattc tgcttcgca ttatgcatt 
tctgtgacc ctcggcttt accacagac cggcacagt 
ttagtggta tcatcaacg gaacctccg aaagaatct 
tcaagtgag gttgccccc aataaggag gatgtggcc 
ccaattgtc ccaaaaatt tacgaaatg aaagttgac 
acaaacgta tcgaatcgc ttcgccaaa acttcagtc 
gtcagtaaa gtgaaaaat ttggcgaaa acaccacag 
gaagccacc ttctcagtc gtgttgccc gaaactgcg 
tacatctcg ggattcgtg atggaaatc gacgggaaa 
agctacaaa gcgtacgtg aaagaaaaa gaagaagcg 
aagcaaatt tacaatgag gctgtcgga agaggccaa 
gcagcggct catgtcgaa gctaacgcc agagattcc 
aacagattc actgtttct ctaaacatc gaaccgcaa 
aagaaagca gttttcact ctcacgtac gaagaactg 
ctccagcgt caaaacgag cagtacgaa gttgtaatt 
aacatccat ccaggccag ccggtcaag gacttaaac 
gtcgaggta cacattgac gaaagcagg cctttgaaa 
ttcgttaag tctccgcct ttgcgcacc ggcaacgaa 
atatccaaa aacgacgac aaaacggcc tcgttggcc 
gaaatcaag caaaataac tcaacttcg gcaacggtc 
aaattcaac ccaaacatc gaacgccag aagcaactg 
gccactggt ttggggacg aaagaggag aatgggcta 
gccggccag ttcgtggtg caatacgac gttgaacga 
gaccccaaa gggggcgag gttctgctc aaggatggc 
tatttcgtc catttcttt gcccccagt gaggtggaa 
gccctaccc aagcaagtt attttcgtc ctggatact 
agtggcagc atggacgga aacagaatc aagcagttg 
aaagaggca atgaacagc attttgagc gaattgaag 
aaggaagat gtgttcaat attgtggaa ttcagttcc 
attgtgaaa gtctggaac gttgacaag gtccaagtc 
gattacgaa gttggcgaa gacccgtgg ccgctgtac 
gacagtcca gaggcccct cagaagaac aaaacaaac 
caggtgttg cccccggcc tacaaagcc accgatgaa 
aataaagaa aaggcgaag aaagttgtg gaaaagttg 
aatgcgtac ggaggcact gatattaaa tcggcactg 
gaggttggt ttgaaactt gtcaagaag aacaaagaa 
aacaaggaa gatgcacat cagccgatt attgtgttt 
ttgaccgac ggtgagcca accatgggc gaaaccaac 
acggagaaa atcacatct gctatttct gaaatgaac 
agtggggaa acgcgcgcc cccattttc tcgctttct 
tttggcgat ggcgctgat cgggaattc ttgcaaaaa 
atatctttg aagaatttg ggtttcgcg agacacatt 
tacgaagct gccgatgcc agtctccag ttgcaagaa 
ttctacaaa caaatttcg tcgccactg ttgaacaat 
gttaacttc aaatacgtc tcaaatgtg acaaaactg 
accaaaacc gtgtttcca atttacttc cgaggatcg 
gaaatcgta gtcgctggc gtggcagag gacgaattc 
ctaccaccc tccatcgag ggaaatact ctacgggga 
ccgatcatc tggagtccg aaagttgag acgcctgtt 
ggcgacctg gagagactt tgggcttat ctcacaatt 
aagcaaata ctcgaagct agggatgcc gctgagaag 
aaggagccc tacaccaaa aaggcgcta gatattgct 
ttgaaatac tcatttgtg accccggtt agttcattg 
gtggtggtc aagccaaac gatacgagt gctgtggac 
acggaggat gcgtcgaaa accgccgac aggccgtac 
tttttaaac agaaattat gcaacacct aatttaaga 
ccccaatta ggtctggca ggcccatca actctttcc 
tttgcagcg gccgcagct gggtttggg ggtgccgcc 
cctccgatg ccccaacct gctgttcaa gaagccgat 
cttgaggaa gatgcatac atgtcacct gaaatgttt 
gctccatct acaactgac ataccaaca accacaaaa 
tcgcaagat ttgtacgaa gacattaag aataaattg 
ccatggctg aaagatatt ataacagtt aatggaacg 
ataacttta cccaacggg agttacaaa attagcaca 
aatgaaaca caagtgacg tcaacccct caatgtcca 
aaaacaccc ataaatggt accgctggc gtctgcaca 
cttttacca cattgcccg caagtgttt tcgctctta 
acagatgcc aaagttttt caggagttt ttctgtccc 
ttggataca tttgttgga gtgtgttgt ccaattgca 
acagatgct acaagcgca agtgcagca atttcttaa 
tatacttat tctgtgatt actgatgtt tatgtacta 
taattttaa gactagcta ataaagcgt atattttac 
t

      If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
      Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY