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Gene information
General information
TC014157 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6802250..6805919
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014157
      iBeetle screen iB_10013

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      larva segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      head appendages structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      legs size shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
      legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

      legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
      posterior terminus not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014157 RA
        MKSKLKLGF CFALCISVT LGFTTDRHS LVVSSTEPP 
        KESSSEVAP NKEDVAPIV PKIYEMKVD TNVSNRFAK 
        TSVVSKVKN LAKTPQEAT FSVVLPETA YISGFVMEI 
        DGKSYKAYV KEKEEAKQI YNEAVGRGQ AAAHVEANA 
        RDSNRFTVS LNIEPQKKA VFTLTYEEL LQRQNEQYE 
        VVINIHPGQ PVKDLNVEV HIDESRPLK FVKSPPLRT 
        GNEISKNDD KTASLAEIK QNNSTSATV KFNPNIERQ 
        KQLATGLGT KEENGLAGQ FVVQYDVER DPKGGEVLL 
        KDGYFVHFF APSEVEALP KQVIFVLDT SGSMDGNRI 
        KQLKEAMNS ILSELKKED VFNIVEFSS IVKVWNVDK 
        VQVDYEVGE DPWPLYDSP EAPQKNKTN QVLPPAYKA 
        TDENKEKAK KVVEKLNAY GGTDIKSAL EVGLKLVKK 
        NKENKEDAH QPIIVFLTD GEPTMGETN TEKITSAIS 
        EMNSGETRA PIFSLSFGD GADREFLQK ISLKNLGFA 
        RHIYEAADA SLQLQEFYK QISSPLLNN VNFKYVSNV 
        TKLTKTVFP IYFRGSEIV VAGVAEDEF LPPSIEGNT 
        LRGPIIWSP KVETPVGDL ERLWAYLTI KQILEARDA 
        AEKKEPYTK KALDIALKY SFVTPVSSL VVVKPNDTS 
        AVDTEDASK TADRPYFLN RNYATPNLR PQLGLAGPS 
        TLSFAAAAA GFGGAAPPM PQPAVQEAD LEEDAYMSP 
        EMFAPSTTD IPTTTKSQD LYEDIKNKL PWLKDIITV 
        NGTITLPNG SYKISTNET QVTSTPQCP KTPINGTAG 
        VCTLLPHCP QVFSLLTDA KVFQEFFCP LDTFVGVCC 
        PIATDATSA SAAIS
      CDS sequence
      • >TC014157 RA
        atgaaaagt aaactcaaa ctaggattc tgcttcgca 
        ttatgcatt tctgtgacc ctcggcttt accacagac 
        cggcacagt ttagtggta tcatcaacg gaacctccg 
        aaagaatct tcaagtgag gttgccccc aataaggag 
        gatgtggcc ccaattgtc ccaaaaatt tacgaaatg 
        aaagttgac acaaacgta tcgaatcgc ttcgccaaa 
        acttcagtc gtcagtaaa gtgaaaaat ttggcgaaa 
        acaccacag gaagccacc ttctcagtc gtgttgccc 
        gaaactgcg tacatctcg ggattcgtg atggaaatc 
        gacgggaaa agctacaaa gcgtacgtg aaagaaaaa 
        gaagaagcg aagcaaatt tacaatgag gctgtcgga 
        agaggccaa gcagcggct catgtcgaa gctaacgcc 
        agagattcc aacagattc actgtttct ctaaacatc 
        gaaccgcaa aagaaagca gttttcact ctcacgtac 
        gaagaactg ctccagcgt caaaacgag cagtacgaa 
        gttgtaatt aacatccat ccaggccag ccggtcaag 
        gacttaaac gtcgaggta cacattgac gaaagcagg 
        cctttgaaa ttcgttaag tctccgcct ttgcgcacc 
        ggcaacgaa atatccaaa aacgacgac aaaacggcc 
        tcgttggcc gaaatcaag caaaataac tcaacttcg 
        gcaacggtc aaattcaac ccaaacatc gaacgccag 
        aagcaactg gccactggt ttggggacg aaagaggag 
        aatgggcta gccggccag ttcgtggtg caatacgac 
        gttgaacga gaccccaaa gggggcgag gttctgctc 
        aaggatggc tatttcgtc catttcttt gcccccagt 
        gaggtggaa gccctaccc aagcaagtt attttcgtc 
        ctggatact agtggcagc atggacgga aacagaatc 
        aagcagttg aaagaggca atgaacagc attttgagc 
        gaattgaag aaggaagat gtgttcaat attgtggaa 
        ttcagttcc attgtgaaa gtctggaac gttgacaag 
        gtccaagtc gattacgaa gttggcgaa gacccgtgg 
        ccgctgtac gacagtcca gaggcccct cagaagaac 
        aaaacaaac caggtgttg cccccggcc tacaaagcc 
        accgatgaa aataaagaa aaggcgaag aaagttgtg 
        gaaaagttg aatgcgtac ggaggcact gatattaaa 
        tcggcactg gaggttggt ttgaaactt gtcaagaag 
        aacaaagaa aacaaggaa gatgcacat cagccgatt 
        attgtgttt ttgaccgac ggtgagcca accatgggc 
        gaaaccaac acggagaaa atcacatct gctatttct 
        gaaatgaac agtggggaa acgcgcgcc cccattttc 
        tcgctttct tttggcgat ggcgctgat cgggaattc 
        ttgcaaaaa atatctttg aagaatttg ggtttcgcg 
        agacacatt tacgaagct gccgatgcc agtctccag 
        ttgcaagaa ttctacaaa caaatttcg tcgccactg 
        ttgaacaat gttaacttc aaatacgtc tcaaatgtg 
        acaaaactg accaaaacc gtgtttcca atttacttc 
        cgaggatcg gaaatcgta gtcgctggc gtggcagag 
        gacgaattc ctaccaccc tccatcgag ggaaatact 
        ctacgggga ccgatcatc tggagtccg aaagttgag 
        acgcctgtt ggcgacctg gagagactt tgggcttat 
        ctcacaatt aagcaaata ctcgaagct agggatgcc 
        gctgagaag aaggagccc tacaccaaa aaggcgcta 
        gatattgct ttgaaatac tcatttgtg accccggtt 
        agttcattg gtggtggtc aagccaaac gatacgagt 
        gctgtggac acggaggat gcgtcgaaa accgccgac 
        aggccgtac tttttaaac agaaattat gcaacacct 
        aatttaaga ccccaatta ggtctggca ggcccatca 
        actctttcc tttgcagcg gccgcagct gggtttggg 
        ggtgccgcc cctccgatg ccccaacct gctgttcaa 
        gaagccgat cttgaggaa gatgcatac atgtcacct 
        gaaatgttt gctccatct acaactgac ataccaaca 
        accacaaaa tcgcaagat ttgtacgaa gacattaag 
        aataaattg ccatggctg aaagatatt ataacagtt 
        aatggaacg ataacttta cccaacggg agttacaaa 
        attagcaca aatgaaaca caagtgacg tcaacccct 
        caatgtcca aaaacaccc ataaatggt accgctggc 
        gtctgcaca cttttacca cattgcccg caagtgttt 
        tcgctctta acagatgcc aaagttttt caggagttt 
        ttctgtccc ttggataca tttgttgga gtgtgttgt 
        ccaattgca acagatgct acaagcgca agtgcagca 
        atttcttaa
      mRNA sequence
      • >TC014157 RA
        ttggtcatc ttatattgt tactgtagt gtgattatt 
        tttttaact gttaagtag aataaaaaa atgaaaagt 
        aaactcaaa ctaggattc tgcttcgca ttatgcatt 
        tctgtgacc ctcggcttt accacagac cggcacagt 
        ttagtggta tcatcaacg gaacctccg aaagaatct 
        tcaagtgag gttgccccc aataaggag gatgtggcc 
        ccaattgtc ccaaaaatt tacgaaatg aaagttgac 
        acaaacgta tcgaatcgc ttcgccaaa acttcagtc 
        gtcagtaaa gtgaaaaat ttggcgaaa acaccacag 
        gaagccacc ttctcagtc gtgttgccc gaaactgcg 
        tacatctcg ggattcgtg atggaaatc gacgggaaa 
        agctacaaa gcgtacgtg aaagaaaaa gaagaagcg 
        aagcaaatt tacaatgag gctgtcgga agaggccaa 
        gcagcggct catgtcgaa gctaacgcc agagattcc 
        aacagattc actgtttct ctaaacatc gaaccgcaa 
        aagaaagca gttttcact ctcacgtac gaagaactg 
        ctccagcgt caaaacgag cagtacgaa gttgtaatt 
        aacatccat ccaggccag ccggtcaag gacttaaac 
        gtcgaggta cacattgac gaaagcagg cctttgaaa 
        ttcgttaag tctccgcct ttgcgcacc ggcaacgaa 
        atatccaaa aacgacgac aaaacggcc tcgttggcc 
        gaaatcaag caaaataac tcaacttcg gcaacggtc 
        aaattcaac ccaaacatc gaacgccag aagcaactg 
        gccactggt ttggggacg aaagaggag aatgggcta 
        gccggccag ttcgtggtg caatacgac gttgaacga 
        gaccccaaa gggggcgag gttctgctc aaggatggc 
        tatttcgtc catttcttt gcccccagt gaggtggaa 
        gccctaccc aagcaagtt attttcgtc ctggatact 
        agtggcagc atggacgga aacagaatc aagcagttg 
        aaagaggca atgaacagc attttgagc gaattgaag 
        aaggaagat gtgttcaat attgtggaa ttcagttcc 
        attgtgaaa gtctggaac gttgacaag gtccaagtc 
        gattacgaa gttggcgaa gacccgtgg ccgctgtac 
        gacagtcca gaggcccct cagaagaac aaaacaaac 
        caggtgttg cccccggcc tacaaagcc accgatgaa 
        aataaagaa aaggcgaag aaagttgtg gaaaagttg 
        aatgcgtac ggaggcact gatattaaa tcggcactg 
        gaggttggt ttgaaactt gtcaagaag aacaaagaa 
        aacaaggaa gatgcacat cagccgatt attgtgttt 
        ttgaccgac ggtgagcca accatgggc gaaaccaac 
        acggagaaa atcacatct gctatttct gaaatgaac 
        agtggggaa acgcgcgcc cccattttc tcgctttct 
        tttggcgat ggcgctgat cgggaattc ttgcaaaaa 
        atatctttg aagaatttg ggtttcgcg agacacatt 
        tacgaagct gccgatgcc agtctccag ttgcaagaa 
        ttctacaaa caaatttcg tcgccactg ttgaacaat 
        gttaacttc aaatacgtc tcaaatgtg acaaaactg 
        accaaaacc gtgtttcca atttacttc cgaggatcg 
        gaaatcgta gtcgctggc gtggcagag gacgaattc 
        ctaccaccc tccatcgag ggaaatact ctacgggga 
        ccgatcatc tggagtccg aaagttgag acgcctgtt 
        ggcgacctg gagagactt tgggcttat ctcacaatt 
        aagcaaata ctcgaagct agggatgcc gctgagaag 
        aaggagccc tacaccaaa aaggcgcta gatattgct 
        ttgaaatac tcatttgtg accccggtt agttcattg 
        gtggtggtc aagccaaac gatacgagt gctgtggac 
        acggaggat gcgtcgaaa accgccgac aggccgtac 
        tttttaaac agaaattat gcaacacct aatttaaga 
        ccccaatta ggtctggca ggcccatca actctttcc 
        tttgcagcg gccgcagct gggtttggg ggtgccgcc 
        cctccgatg ccccaacct gctgttcaa gaagccgat 
        cttgaggaa gatgcatac atgtcacct gaaatgttt 
        gctccatct acaactgac ataccaaca accacaaaa 
        tcgcaagat ttgtacgaa gacattaag aataaattg 
        ccatggctg aaagatatt ataacagtt aatggaacg 
        ataacttta cccaacggg agttacaaa attagcaca 
        aatgaaaca caagtgacg tcaacccct caatgtcca 
        aaaacaccc ataaatggt accgctggc gtctgcaca 
        cttttacca cattgcccg caagtgttt tcgctctta 
        acagatgcc aaagttttt caggagttt ttctgtccc 
        ttggataca tttgttgga gtgtgttgt ccaattgca 
        acagatgct acaagcgca agtgcagca atttcttaa 
        tatacttat tctgtgatt actgatgtt tatgtacta 
        taattttaa gactagcta ataaagcgt atattttac 
        t
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