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Gene information
General information
TC004141 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1592298..1598080
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004141
    iBeetle screen iB_10011

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004141 RA
      MTILPAKRP SANPEEKSD NSQQNGNSE RAVRRRDDI 
      SSLNEASPS SRRSQFPYV KSTTERKER LRKRYKEPD 
      RARDRERER EQKREVRRE QKRERVAKR DISNEPLHD 
      NNASNSVVD SVAVEPTCN NNEPGTANK EDYTGFNSE 
      DEYDDQGLL KERNIPDEE WQKRDVLFS RGMSNLGFE 
      IKQMNEDGA CLFRSIADQ VYGDQEFHY QVRQDCMNY 
      IVQNRDYFE PYVTEDFDK YVARKRIWN VHGNHLEIQ 
      AMSELYNRT IEVYCYQIT PINIFNGSR LINSYEPIR 
      LSYHRMCHY NSISNPNKP SVGVGLGLP NYHTVDIDR 
      RRFNDALRA SDELLIEQT MLEDKLKAT DFEATNEAI 
      EEQVARESY IQYFRDSER RLKNQGHSC GSSSTITSA 
      TMSSSRSSR KGSVSPKGS QSPKGSNSP KTTPLASPR 
      GAGAPSPST SRYEADVSG GDVAGACGG PPEEFPKFS 
      PRRSEPPAE DVNNPDYDE NFNEDFKFD FDSDSDQSA 
      GAGPSNRGD IDYDNYDQE IMAQVLAES RKTYLAELK 
      QRSKKRHGS PGPSTSS
    CDS sequence
    • >TC004141 RA
      atgacaatt ttaccagcc aaaagacct tcggcgaat 
      ccggaggag aaaagcgac aatagtcag caaaatggg 
      aattccgag cgagcggtg cgtaggaga gacgatata 
      agttctctg aatgaggca agcccttca agccgccga 
      tctcagttc ccctacgtc aagtcaaca actgagcgg 
      aaagagcgt ttgaggaag cgatataaa gagccagat 
      cgggcgcga gatagggaa cgcgagcgc gaacaaaaa 
      cgggaagtt cggagggaa caaaaacgg gaacgagtt 
      gctaaacgc gatatctca aatgagcct ctacatgac 
      aataatgcg agtaattcg gttgttgac tcagtcgct 
      gttgagccc acttgcaac aacaacgaa cctgggact 
      gcgaacaag gaagactac acgggcttc aacagcgag 
      gatgagtac gatgaccag ggactcctc aaagagcgg 
      aacataccg gatgaagag tggcaaaaa agggacgtg 
      ttattttcc cgcggcatg tccaacctt gggttcgaa 
      atcaagcaa atgaacgaa gatggggcc tgtctcttc 
      cggtcgata gcagaccaa gtgtacggc gaccaagaa 
      ttccattat caagtcagg caggactgc atgaactac 
      atcgttcaa aaccgtgac tactttgaa ccttacgtg 
      acggaggat tttgataaa tacgttgca cggaaacgt 
      atatggaac gtacatggc aaccattta gaaatacaa 
      gcaatgagc gagttatat aataggaca atcgaagtt 
      tattgctac caaataaca ccaatcaat atattcaat 
      ggttcaaga ttaattaac tcatatgag cctattaga 
      ttatcatac cacaggatg tgtcattat aattctatt 
      agtaaccca aataaacct tcagtaggg gtaggctta 
      ggtttaccc aattaccat accgttgat attgacaga 
      agaaggttc aatgacgct ctgcgggct agtgatgag 
      ttgctaatt gaacagact atgctagaa gacaagttg 
      aaagcaacc gactttgaa gccaccaac gaagccatc 
      gaagaacag gtagctcgc gagtcctac atccaatat 
      tttcgcgac tcagaacgc cgtttgaag aaccaagga 
      cactcctgt ggtagcagt tcaacgata acatcagca 
      accatgtcc agctcgcgc tcgtcgagg aaaggctcg 
      gtctccccg aagggatcg cagtccccc aaagggtcc 
      aactcgcca aagacaaca ccactagcg agcccccga 
      ggcgctggg gcgccgtcc ccatcgact agccgctat 
      gaggccgac gtcagcggg ggcgatgtg gcgggagcc 
      tgcggtgga ccaccggag gaatttccg aaattttcg 
      ccgcgccgt tctgagccc ccggccgag gatgttaat 
      aacccggac tatgatgaa aatttcaac gaggacttt 
      aaatttgat tttgattcc gattcggat cagtcggct 
      ggggccggg ccatcaaac cgcggtgat atagattac 
      gacaattat gaccaggaa atcatggcc caagtgctg 
      gccgagtcg cgcaaaact tacttagct gaacttaag 
      cagcgatcc aagaagcgt catggatct ccgggacca 
      tccacttca tcctag
    mRNA sequence
    • >TC004141 RA
      gtgtgttga cagtagttg agagatttg tcaaactat 
      agaaaataa ttcaataat tttgtagta ttttctgta 
      ttttgaaaa attagacaa tgacaattt taccagcca 
      aaagacctt cggcgaatc cggaggaga aaagcgaca 
      atagtcagc aaaatggga attccgagc gagcggtgc 
      gtaggagag acgatataa gttctctga atgaggcaa 
      gcccttcaa gccgccgat ctcagttcc cctacgtca 
      agtcaacaa ctgagcgga aagagcgtt tgaggaagc 
      gatataaag agccagatc gggcgcgag atagggaac 
      gcgagcgcg aacaaaaac gggaagttc ggagggaac 
      aaaaacggg aacgagttg ctaaacgcg atatctcaa 
      atgagcctc tacatgaca ataatgcga gtaattcgg 
      ttgttgact cagtcgctg ttgagccca cttgcaaca 
      acaacgaac ctgggactg cgaacaagg aagactaca 
      cgggcttca acagcgagg atgagtacg atgaccagg 
      gactcctca aagagcgga acataccgg atgaagagt 
      ggcaaaaaa gggacgtgt tattttccc gcggcatgt 
      ccaaccttg ggttcgaaa tcaagcaaa tgaacgaag 
      atggggcct gtctcttcc ggtcgatag cagaccaag 
      tgtacggcg accaagaat tccattatc aagtcaggc 
      aggactgca tgaactaca tcgttcaaa accgtgact 
      actttgaac cttacgtga cggaggatt ttgataaat 
      acgttgcac ggaaacgta tatggaacg tacatggca 
      accatttag aaatacaag caatgagcg agttatata 
      ataggacaa tcgaagttt attgctacc aaataacac 
      caatcaata tattcaatg gttcaagat taattaact 
      catatgagc ctattagat tatcatacc acaggatgt 
      gtcattata attctatta gtaacccaa ataaacctt 
      cagtagggg taggcttag gtttaccca attaccata 
      ccgttgata ttgacagaa gaaggttca atgacgctc 
      tgcgggcta gtgatgagt tgctaattg aacagacta 
      tgctagaag acaagttga aagcaaccg actttgaag 
      ccaccaacg aagccatcg aagaacagg tagctcgcg 
      agtcctaca tccaatatt ttcgcgact cagaacgcc 
      gtttgaaga accaaggac actcctgtg gtagcagtt 
      caacgataa catcagcaa ccatgtcca gctcgcgct 
      cgtcgagga aaggctcgg tctccccga agggatcgc 
      agtccccca aagggtcca actcgccaa agacaacac 
      cactagcga gcccccgag gcgctgggg cgccgtccc 
      catcgacta gccgctatg aggccgacg tcagcgggg 
      gcgatgtgg cgggagcct gcggtggac caccggagg 
      aatttccga aattttcgc cgcgccgtt ctgagcccc 
      cggccgagg atgttaata acccggact atgatgaaa 
      atttcaacg aggacttta aatttgatt ttgattccg 
      attcggatc agtcggctg gggccgggc catcaaacc 
      gcggtgata tagattacg acaattatg accaggaaa 
      tcatggccc aagtgctgg ccgagtcgc gcaaaactt 
      acttagctg aacttaagc agcgatcca agaagcgtc 
      atggatctc cgggaccat ccacttcat cctagttta 
      aatactaaa ttaattgag ttacatgtt cgtgctttc 
      gtttcattt ttagttaat taaggttca ttgtttgct 
      tttcaaggt atgcaattg ctttgtttt gttatataa 
      aatgcatat cttgtaaaa ttgtattgg catgttata 
      atcgttttt tagttaaaa cgaccttcc tccacccag 
      aagattagt ttcgttttt ttggagaaa gagctttcg 
      tttctagta ccgtagttt cgattttgc atggattgt 
      gggggggtc gtttgtaaa aaaaaattc aaaatgtta 
      tattttgag acactgttt cacggatgt aagtttcgc 
      cctctaaat ttcagtgtc aaaaagtat tttatttat 
      gttttcaga tatctataa gtatatgta cttatgagt 
      tccaatgta tttcagttt cgttcaaat aaaattgtt 
      gtgatatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY