Status
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Gene information
General information
TC030756 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6238511..6242576
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030756
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10008

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030756 RA
      MWAFLPYPA LYKIFQYLN YKELVRVSE VCHNWCEVS 
      RDDFLWKDL FFENFLVDK SVPIVSERT WYEEFKRLS 
      YNVPVVQTE TLHDHRHQV LHVSFSHNG KYFATCSKD 
      GYVLLWNST YPVEIKYSR DMNSFSWKY TQYSQFNES 
      DTLLLVSGV HFGTPHSTS GEIAVFNLL AGFELQCRV 
      VNKPYDIYG TWYSDQHLL SGDLHWLAH LVSTSSLWL 
      NKASQEAES EHVPITHNM FRFYNRNAS SIRAIMIAN 
      CLPNSELAT ETKETEVEV GPSENKNST FEHGNPVSH 
      RDINAVPCT HSSSGSAIS FDAKKTASG SDLGNPIWY 
      NSDYRKVES ENMLIDESE EETEDDDTG KDDCDDEGS 
      EEDSDQLTQ NDKYLIFTT GFKTYTPHQ IGFKRIKPF 
      AFPKRLDPG PSLKERIIL RERKRELLK SGSPPPEPN 
      WLDFDSVAD KFDKVDHVI DLHGHIVGM GLSPDHRYL 
      YVNSRSWPE GYKISNPLD PPPIAQEID IHVIDLVTL 
      KQVGTMLRA HKAYTSNSE CFFIFLDVC NQYVASGAE 
      DKHGYLWDR HYGMCLVKF PHSDVVNSV AFNPKDPEM 
      LVTTSDDHI IKVWRSRAK VRELKLSET QFPKGVEFR 
      RSRTKDKLR CNAGIRRI
    CDS sequence
    • >TC030756 RA
      atgtgggct tttttaccc tatcctgct ttatacaaa 
      atcttccag tacttgaat tataaagaa ttagtgcgt 
      gtaagtgaa gtttgtcat aactggtgt gaggttagt 
      cgagatgat tttctctgg aaagacttg ttctttgag 
      aactttctt gtcgataaa tctgtaccg atagtttct 
      gaaagaact tggtatgag gaattcaaa agactatct 
      tacaatgtt ccagtggtc caaacagag acattacac 
      gatcatcga catcaagtc cttcatgtc agtttttca 
      cacaacggc aaatacttt gccacatgc tcaaaggat 
      ggctatgtc ctgctatgg aactccaca tatccagtt 
      gaaataaaa tattcacga gatatgaac agtttttcc 
      tggaaatac acacaatat tcacagttt aatgaatct 
      gacactctg cttcttgta tcaggagtt cattttggt 
      acaccacat agtacttca ggcgaaatt gcagtattt 
      aatttactc gctgggttt gagttgcaa tgtcgagtc 
      gtcaataag ccatatgat atttatggc acttggtac 
      agtgaccaa cacttactc tcaggtgac ttacattgg 
      ttagcacat ttagttagt acgtcctcg ttatggtta 
      aataaagca tcacaagag gctgaatca gagcatgtc 
      ccgataact cataacatg tttcggttt tataacaga 
      aatgcaagt tcaattaga gcgataatg atcgccaac 
      tgtcttcca aattctgaa cttgcgaca gagaccaaa 
      gaaaccgaa gttgaggtc ggaccgtct gaaaataaa 
      aacagcaca tttgagcac gggaaccct gtaagccat 
      agagacata aatgcggtt ccctgcaca cacagttct 
      tctggcagt gctatttct tttgatgcc aaaaaaact 
      gcaagtggc tcagactta ggaaatccg atttggtat 
      aattcagat tatcgcaaa gttgaatct gagaatatg 
      ctaatagat gagtctgag gaagaaacg gaagatgat 
      gacactggc aaggatgat tgtgatgat gaaggttct 
      gaagaagac tcagatcaa ctcactcaa aacgataaa 
      tatttaatt tttacaacg ggctttaaa acatacaca 
      cctcatcag ataggcttt aaacggata aaaccgttc 
      gctttccct aaacgcttg gacccgggc ccctcgttg 
      aaagaacgc ataattctg cgtgaacgc aaacgcgag 
      cttttaaaa tccggttcg ccgcccccc gagccaaac 
      tggctagat ttcgactca gtggccgat aaatttgac 
      aaagtcgac catgtgatt gatttacat ggccatatc 
      gtcggaatg ggcctcagt cctgaccac agatacctc 
      tacgtcaac agcagatcc tggcctgaa gggtacaaa 
      atatcgaat cccttggat ccgcccccg attgcacaa 
      gaaatcgac atccacgtt attgactta gtgactttg 
      aaacaagtc ggtactatg ctacgagcg cacaaggcg 
      tacacttcc aacagcgag tgctttttt atatttttg 
      gacgtttgc aaccagtat gtggcgagt ggcgctgaa 
      gataagcat gggtacttg tgggatcga cactacggc 
      atgtgttta gttaagttc ccgcattcg gatgtggtt 
      aattccgta gcatttaat cccaaagac ccggaaatg 
      ttggttact acgtcagat gatcacatc attaaggtg 
      tggaggtca agggccaaa gtgagagaa ctgaaattg 
      agcgaaacg cagtttcca aaaggtgtg gagtttaga 
      cgaagtcga acgaaagat aagttaaga tgcaacgcc 
      ggcataagg aggatatga
    mRNA sequence
    • >TC030756 RA
      agtaaataa ataacacaa tcacaaaaa acaaataat 
      aaaacaaag aggaggatt atgtgggct tttttaccc 
      tatcctgct ttatacaaa atcttccag tacttgaat 
      tataaagaa ttagtgcgt gtaagtgaa gtttgtcat 
      aactggtgt gaggttagt cgagatgat tttctctgg 
      aaagacttg ttctttgag aactttctt gtcgataaa 
      tctgtaccg atagtttct gaaagaact tggtatgag 
      gaattcaaa agactatct tacaatgtt ccagtggtc 
      caaacagag acattacac gatcatcga catcaagtc 
      cttcatgtc agtttttca cacaacggc aaatacttt 
      gccacatgc tcaaaggat ggctatgtc ctgctatgg 
      aactccaca tatccagtt gaaataaaa tattcacga 
      gatatgaac agtttttcc tggaaatac acacaatat 
      tcacagttt aatgaatct gacactctg cttcttgta 
      tcaggagtt cattttggt acaccacat agtacttca 
      ggcgaaatt gcagtattt aatttactc gctgggttt 
      gagttgcaa tgtcgagtc gtcaataag ccatatgat 
      atttatggc acttggtac agtgaccaa cacttactc 
      tcaggtgac ttacattgg ttagcacat ttagttagt 
      acgtcctcg ttatggtta aataaagca tcacaagag 
      gctgaatca gagcatgtc ccgataact cataacatg 
      tttcggttt tataacaga aatgcaagt tcaattaga 
      gcgataatg atcgccaac tgtcttcca aattctgaa 
      cttgcgaca gagaccaaa gaaaccgaa gttgaggtc 
      ggaccgtct gaaaataaa aacagcaca tttgagcac 
      gggaaccct gtaagccat agagacata aatgcggtt 
      ccctgcaca cacagttct tctggcagt gctatttct 
      tttgatgcc aaaaaaact gcaagtggc tcagactta 
      ggaaatccg atttggtat aattcagat tatcgcaaa 
      gttgaatct gagaatatg ctaatagat gagtctgag 
      gaagaaacg gaagatgat gacactggc aaggatgat 
      tgtgatgat gaaggttct gaagaagac tcagatcaa 
      ctcactcaa aacgataaa tatttaatt tttacaacg 
      ggctttaaa acatacaca cctcatcag ataggcttt 
      aaacggata aaaccgttc gctttccct aaacgcttg 
      gacccgggc ccctcgttg aaagaacgc ataattctg 
      cgtgaacgc aaacgcgag cttttaaaa tccggttcg 
      ccgcccccc gagccaaac tggctagat ttcgactca 
      gtggccgat aaatttgac aaagtcgac catgtgatt 
      gatttacat ggccatatc gtcggaatg ggcctcagt 
      cctgaccac agatacctc tacgtcaac agcagatcc 
      tggcctgaa gggtacaaa atatcgaat cccttggat 
      ccgcccccg attgcacaa gaaatcgac atccacgtt 
      attgactta gtgactttg aaacaagtc ggtactatg 
      ctacgagcg cacaaggcg tacacttcc aacagcgag 
      tgctttttt atatttttg gacgtttgc aaccagtat 
      gtggcgagt ggcgctgaa gataagcat gggtacttg 
      tgggatcga cactacggc atgtgttta gttaagttc 
      ccgcattcg gatgtggtt aattccgta gcatttaat 
      cccaaagac ccggaaatg ttggttact acgtcagat 
      gatcacatc attaaggtg tggaggtca agggccaaa 
      gtgagagaa ctgaaattg agcgaaacg cagtttcca 
      aaaggtgtg gagtttaga cgaagtcga acgaaagat 
      aagttaaga tgcaacgcc ggcataagg aggatatga 
      ggttgtggt ggcaataat tgtgtcttc gtgtgaaca 
      taatccagt ttttcgagt cgtttgatt gtactttta 
      acttattag tttatccgt ccaaatatg aaggaatgt 
      ttttatgac taacataat cttgaatat atgaaaata 
      aaaaaatat atattgcct tatttattt ttcataaag 
      tcctaaata ttggtcata aatattgta atactttaa 
      tgttttcac acctcgtga gattcgttg ttattattt 
      aatatactt tcatcagag cccttatta ccatctgtg 
      ttactatca tcttgagtt tgatgcctc ttcttcttt 
      ttttccaca tgaccttta cagtgctca cactcgtca 
      tcagatcct tcctccgaa ctgctttca tcaaaattt 
      cgcggtttt ttatatata caacaacct acaaaactg 
      aaactttac acgataaca cgaacaaac acctacatt 
      ttgatttct ttttattca tatgttcgt tgtctactg 
      tttcagttg tccactcca cttttctgt cactcttgg 
      gtttagtta gcctcagct taagtgtag gcacttcat 
      gatcctgca agagcttga aattatcac tatttactc 
      ttgcaacgt tttctttgt acaccatta aatttttat 
      catgatt
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