Status
Please wait ...
Gene information
General information
TC033075 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6068341..6073541
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033075
    iBeetle screen iB_10007

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content split - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033075 RA
      MKKCSDTVV LLFIISYSF TFVQSEITL 
      GETSCGQKK CKRDEYCTS YTKACEPCS 
      KICDKSTHN FDETECVKK CQDYIHDSR 
      YVLKDETGT GDQNLRATV QQLSHMVTV 
      TLTLVCLML VILATVLVL QMYRWKKKK 
      NITLATIKN KLFSKKADS AQNKSTPVS 
      NQVKNEKPD LRLEISPES THSGHSPVT 
      VTTSISRRP AEDSTLDYA YDNPAMSSS 
      PNKKNDPNN SF
    CDS sequence
    • >TC033075 RA
      atgaaaaag tgtagtgat actgttgtt ttgttgttc 
      ataatatcg tacagtttt acgtttgtc caaagtgaa 
      attacactc ggcgaaact tcctgtggc caaaaaaaa 
      tgcaaaagg gacgaatac tgcaccagc tatacgaaa 
      gcctgtgaa ccgtgttca aaaatctgc gataaatcc 
      acgcacaat tttgatgaa acggaatgc gtgaaaaaa 
      tgtcaagat tacattcac gattctcgt tatgtccta 
      aaagacgaa actggaact ggagaccaa aacctccga 
      gcaactgtt caacagctt tcccacatg gttacagtc 
      actctcact ttagtatgt ttgatgcta gttatcctc 
      gcaacagtt ttggttcta caaatgtac cgatggaag 
      aaaaagaaa aacattaca ttagcaaca attaaaaac 
      aaattattt tctaaaaag gctgattcc gctcagaac 
      aaatccaca cccgtatca aatcaagtt aaaaacgaa 
      aaacctgat ttacgtctg gaaatttct ccagaatct 
      actcactct ggacacagt cctgttacg gtaacaact 
      tcaataagt cgaaggccg gcagaagat agcaccttg 
      gactacgcg tatgataat ccagccatg tcttccagt 
      cccaacaaa aaaaacgat ccaaataat tcgttctaa
    mRNA sequence
    • >TC033075 RA
      acaaggtgt acaaatccg acataacta cataagaga 
      acaatgact cacacaggt aggtaaggc aaagttagt 
      cagtcaacc attcagtat acacctgtt gcatcgcca 
      tcaaaatgg cttaaaacc aacgtgtga aatccccaa 
      acacttttt ttttaacaa tgtgatatc aaaatcccg 
      ccaaaaaca agttcggta acatcactt ttccaaaaa 
      gtacaaaaa ttggtgctt ccaaagcaa taaaaatga 
      aaaagtgta gtgatactg ttgttttgt tgttcataa 
      tatcgtaca gttttacgt ttgtccaaa gtgaaatta 
      cactcggcg aaacttcct gtggccaaa aaaaatgca 
      aaagggacg aatactgca ccagctata cgaaagcct 
      gtgaaccgt gttcaaaaa tctgcgata aatccacgc 
      acaattttg atgaaacgg aatgcgtga aaaaatgtc 
      aagattaca ttcacgatt ctcgttatg tcctaaaag 
      acgaaactg gaactggag accaaaacc tccgagcaa 
      ctgttcaac agctttccc acatggtta cagtcactc 
      tcactttag tatgtttga tgctagtta tcctcgcaa 
      cagttttgg ttctacaaa tgtaccgat ggaagaaaa 
      agaaaaaca ttacattag caacaatta aaaacaaat 
      tattttcta aaaaggctg attccgctc agaacaaat 
      ccacacccg tatcaaatc aagttaaaa acgaaaaac 
      ctgatttac gtctggaaa tttctccag aatctactc 
      actctggac acagtcctg ttacggtaa caacttcaa 
      taagtcgaa ggccggcag aagatagca ccttggact 
      acgcgtatg ataatccag ccatgtctt ccagtccca 
      acaaaaaaa acgatccaa ataattcgt tctaattgt 
      tttacttgt gtacgtttt atctacaac tagtcggga 
      agctgtgca atttaaaag cttctccct tgcgctaat 
      tcattacta acatcttgt tttttatac tacttaact 
      gttttaata taatttcat aaattttat tactcctaa 
      tactctacg tatttaact caaattaat aatttttaa 
      taataaaag tatgtggat ttattgttg gttgaagct 
      ttaatattc gtacttatt agttttttt aatagtatt 
      agaagttca ccacatata catgccaaa tttttggac 
      accgatatc tatccaaaa atttgtatc gtaaaatgt 
      ggtaactac tttgtagtc gtacctagt gttagccgc 
      atttagcac taaaataag ttttgttag gtgattaca 
      actccatcc atacaaagc attccatta ggtgtagtt 
      tttttagaa gtgtagtga ccaataaat agcatttaa 
      cgccctttg tgagcagct ataagtatt tgtagaacc 
      tgtctttgt ctaaaaaat acaaaattg caacctgga 
      taactgaac ttttttgac gcaatgaca gagttctgt 
      actcgtatg catcactaa ttgttaata aatatactc 
      gtatggtag aatagtcaa tgtgtgata ctagagggt 
      agatgatca ttatgttgt tttattttg tgtgaaatt 
      tattatgtt atttctctt aattattag gtattatct 
      ttacataat agtttacaa aaaaaaaca tcacgtttg 
      tgcacttaa ttgttagtt tattaggtt ttattgtat 
      ttagttaac tttaatttt actttatat tttttttgt 
      ttttttttt aattaagag tcttctgaa tctcttaat 
      ttgcgtcca acaatccgt ccatttaat tttaggtat 
      gcacattaa agaagtaat tctttaatg cttgcatgt 
      aggaatata gttatttac attttaaac tataagtac 
      ttttgtaaa tatctagat attaacaac tatggaaac 
      gtagacact caaaatatg atatttctt tgaaagttt 
      tcagtcatt taattatca agggtttac agttttttt 
      atgaaaaaa ttgctaaaa cgtttttga ataagtttt 
      tttgaagtg tttccatat attcggagt gtattattt 
      tcgtattgt taaactttg tgtattatt attaaaacg 
      tcttgggca tagtactta cttaattct acattttag 
      taattagta taaattaga aaagaaaag tatattata 
      ggtaaccta gtaggagtt aaaaaaaac tatgacatt 
      gcgtgcaaa agtgaatct aaaaaataa attatgtaa 
      atacacata ttacatttt atgttagag tattgcaat 
      aattgaaat aataataaa ctcatagaa caacttcca 
      gaacataac gtatatcta ttttgagat gcattatgt 
      aactacaaa tttattcaa atattatta atattaata 
      attattatt tttatagtt ggataggta tattttttc 
      agtaataga ctgggcttt taaattaag aagaataaa 
      taaaataaa aaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY