Status
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Gene information
General information
TC013685 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6059516..6061831
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013685
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10006

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013685 RA
      MGDDGSWRT PAFRQSVVS KIDEAIRQS GMNTSRNSI 
      EMENHVFQK AKTKEEYLG YVARLILHV REMNTKKGA 
      GMGVPGQNQ GAPGVPDPI NALQTLAIQ GTRNPLMTM 
      GGNQPGPMG AQQQIAASN LLQNLNRGP GQAMNNMQQ 
      GMVQRPNMG MGPNGGPLP NQLGNQMSQ MNTQMANQL 
      AGQMPNQMG NQMQGPVQN QMANQLPGQ MQMGGPMQN 
      SMGPMGNQM PNQMLSQMQ SNKQGPMMM NGPNTGFPR 
      NSTPNQFLS QSPSPSVQS PAGLSGAPP SNQMVASPA 
      LAPSPSSQM GMLGAGPPR SVGMVPSPG SSLNTPGQP 
      NQSPMGMQD EQAYREKVR QLSKYIEPL RKMIARMGN 
      DGEHVEKLS KMKKLLEIL SNPQQRMPL ETLKKCEVV 
      LEKLDFKRS DGSVPTSTS GHLPIKEHH VFNPLLEAV 
      SNNLQSSVI NHTLHRTFG PTLEALFGP EIKLVPPLK 
      KMKLEEPTS DIPDVLQGE IARLDQRFK VSLDPNQQP 
      GSKSIQLTC WLDDKQLPC VPPISLVVP EDYPKSAPS 
      CHMAAHEYN ATQFLSAVQ TALLARIKK LPKYFSVSQ 
      LLDTWEMSV RQASAPTSV PVFPTTLLM GL
    CDS sequence
    • >TC013685 RA
      atgggtgac gatggttct tggagaacc ccagccttt 
      agacaaagt gttgtatca aaaattgat gaagcaatt 
      cgtcagtca ggaatgaac acttcacgc aacagcatt 
      gaaatggaa aatcatgtt ttccagaaa gctaagaca 
      aaagaagaa tatttgggc tacgtggcc cgacttatt 
      ctccacgtc cgcgaaatg aacaccaaa aagggcgct 
      ggaatgggt gtcccaggc caaaatcaa ggagctcct 
      ggcgtgcct gaccccata aacgcatta caaacattg 
      gccattcaa ggcacgaga aaccctttg atgacaatg 
      ggtggcaat cagccaggc ccgatgggc gcccaacag 
      caaattgca gcctcaaat ttactacaa aatttgaac 
      aggggccca ggacaagcc atgaacaac atgcagcaa 
      gggatggta cagagaccc aacatgggc atgggcccc 
      aacggcggc cctctaccc aaccaactg ggcaaccag 
      atgagtcaa atgaacact caaatggcc aaccaactg 
      gctggacaa atgcccaac cagatgggc aaccagatg 
      caaggcccc gtgcagaat caaatggcc aaccaactg 
      cccggtcag atgcagatg gggggccct atgcagaac 
      tccatgggg ccaatgggc aaccaaatg cccaatcaa 
      atgctaagc cagatgcaa tcgaataag caaggccct 
      atgatgatg aatggacct aacaccgga ttccccaga 
      aactcaacc cctaaccag ttcctgagc caaagtccg 
      tcgccctca gtccagtcc ccggctggt ctcagtggg 
      gctccgccc agtaatcag atggtagcg tcgccggct 
      ttggcccca tcgcccagc tcgcaaatg ggcatgttg 
      ggggcaggg cccccaagg tccgtgggc atggtgccc 
      agccctggg agctccctc aacacgccg ggacaacca 
      aaccaaagc cccatggga atgcaggat gaacaggct 
      taccgcgaa aaagtcaga cagttgagc aagtacata 
      gaaccgctg aggaaaatg atcgctagg atggggaac 
      gatggcgaa catgtggaa aagctgagc aaaatgaag 
      aagttgctc gaaattttg tcgaacccg cagcaacga 
      atgccttta gaaacactg aaaaaatgt gaagtggtt 
      ttggagaag cttgacttt aagaggagt gacggaagt 
      gttccaaca tcaacaagt gggcatttg cccatcaag 
      gagcaccac gtgttcaat ccgttattg gaggcagtc 
      agtaataat ttacaaagc tcggttata aatcacacc 
      ctacatcgc acttttggg cccactttg gaggctcta 
      tttgggcca gaaataaag ttggttccc ccattgaaa 
      aagatgaaa ctggaagag cccaccagc gatattccc 
      gatgtactt cagggggaa atcgcgcga ctcgatcag 
      cgatttaaa gtctcactt gatccaaac cagcagcct 
      ggttccaag tcaattcag ctgacttgt tggttggat 
      gataaacaa ttgccatgc gttcctcca atttctttg 
      gtcgttcct gaagactac ccaaaatcg gcaccaagt 
      tgccatatg gccgctcat gaatataac gctactcaa 
      tttttgtca gctgtgcaa acggctctg ttagcgagg 
      ataaagaag ttgccgaag tatttttca gtttctcag 
      ctattggac acgtgggag atgagtgtg aggcaagcc 
      tcggctccg acaagcgtt cccgttttt cccacaact 
      ttactcatg gggctgtag
    mRNA sequence
    • >TC013685 RA
      atttcttta aaaaaatta cttgaaata atagatttt 
      tttaaacaa gcctaacaa tcgttttgg gataaggtt 
      ttaaaaatg ggtgacgat ggttcttgg agaacccca 
      gcctttaga caaagtgtt gtatcaaaa attgatgaa 
      gcaattcgt cagtcagga atgaacact tcacgcaac 
      agcattgaa atggaaaat catgttttc cagaaagct 
      aagacaaaa gaagaatat ttgggctac gtggcccga 
      cttattctc cacgtccgc gaaatgaac accaaaaag 
      ggcgctgga atgggtgtc ccaggccaa aatcaagga 
      gctcctggc gtgcctgac cccataaac gcattacaa 
      acattggcc attcaaggc acgagaaac cctttgatg 
      acaatgggt ggcaatcag ccaggcccg atgggcgcc 
      caacagcaa attgcagcc tcaaattta ctacaaaat 
      ttgaacagg ggcccagga caagccatg aacaacatg 
      cagcaaggg atggtacag agacccaac atgggcatg 
      ggccccaac ggcggccct ctacccaac caactgggc 
      aaccagatg agtcaaatg aacactcaa atggccaac 
      caactggct ggacaaatg cccaaccag atgggcaac 
      cagatgcaa ggccccgtg cagaatcaa atggccaac 
      caactgccc ggtcagatg cagatgggg ggccctatg 
      cagaactcc atggggcca atgggcaac caaatgccc 
      aatcaaatg ctaagccag atgcaatcg aataagcaa 
      ggccctatg atgatgaat ggacctaac accggattc 
      cccagaaac tcaacccct aaccagttc ctgagccaa 
      agtccgtcg ccctcagtc cagtccccg gctggtctc 
      agtggggct ccgcccagt aatcagatg gtagcgtcg 
      ccggctttg gccccatcg cccagctcg caaatgggc 
      atgttgggg gcagggccc ccaaggtcc gtgggcatg 
      gtgcccagc cctgggagc tccctcaac acgccggga 
      caaccaaac caaagcccc atgggaatg caggatgaa 
      caggcttac cgcgaaaaa gtcagacag ttgagcaag 
      tacatagaa ccgctgagg aaaatgatc gctaggatg 
      gggaacgat ggcgaacat gtggaaaag ctgagcaaa 
      atgaagaag ttgctcgaa attttgtcg aacccgcag 
      caacgaatg cctttagaa acactgaaa aaatgtgaa 
      gtggttttg gagaagctt gactttaag aggagtgac 
      ggaagtgtt ccaacatca acaagtggg catttgccc 
      atcaaggag caccacgtg ttcaatccg ttattggag 
      gcagtcagt aataattta caaagctcg gttataaat 
      cacacccta catcgcact tttgggccc actttggag 
      gctctattt gggccagaa ataaagttg gttccccca 
      ttgaaaaag atgaaactg gaagagccc accagcgat 
      attcccgat gtacttcag ggggaaatc gcgcgactc 
      gatcagcga tttaaagtc tcacttgat ccaaaccag 
      cagcctggt tccaagtca attcagctg acttgttgg 
      ttggatgat aaacaattg ccatgcgtt cctccaatt 
      tctttggtc gttcctgaa gactaccca aaatcggca 
      ccaagttgc catatggcc gctcatgaa tataacgct 
      actcaattt ttgtcagct gtgcaaacg gctctgtta 
      gcgaggata aagaagttg ccgaagtat ttttcagtt 
      tctcagcta ttggacacg tgggagatg agtgtgagg 
      caagcctcg gctccgaca agcgttccc gtttttccc 
      acaacttta ctcatgggg ctgtaggct ttattgttg 
      tcttaaaat cttattgtc tagattaga taccaattt 
      ttatgtaaa ataaagcaa taaataaat gtataaaac 
      caaa
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