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Gene information
General information
TC014107 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5735745..5737614
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014107
    iBeetle screen iB_10004

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content split - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014107 RA
      MTKTTEKLT YDNKIILAP MVRVGTLPM RLLSLDHGA 
      DIVYTEELI DWKFLKSIR RENDVLGTV DYLDKSDGT 
      VVFRTCPRE KNRVVVQLG TCDPQRALK VAKMIENDV 
      AGIDINMGC PKEFSLKGG MGAALLKQP EKAKSILST 
      LTQNVKIPI TCKIRVFES AEETLDLVK ELVSTGISA 
      IAIHGRTKA ERPQHPNRN ATIKFIAEN VSIPVVANG 
      GSKEIEKYQ DIIKFKEEC GCSSVMIAR AAEANCSIF 
      RKQGLLDIE TVIEQYLKY AIDYDNSPS NTKYCIQNI 
      LKELQETPR GKKFLECQT LEQICAIWD LADYCRKKQ 
      LQFHSQGIL SRREITPDM FSPKAKKVK TELDDDVIE 
      MKCAFIRVN YPDDPDLPK SKIIAYCGK HKFDLPKYQ 
      TVNEDKLFR AIATFQGKK YSSSYWEKN KRFAEQGAA 
      LVLACSLGL VTKEELIKD GSMLE
    CDS sequence
    • >TC014107 RA
      atgacaaaa actactgaa aaattaact tacgacaac 
      aaaatcata ttggccccc atggtacgt gttggtact 
      ctacccatg agattgttg tcgctggac cacggggcc 
      gacattgtc tacactgaa gaattaatt gactggaaa 
      tttttaaaa tcaataaga cgggaaaat gacgtgttg 
      ggcacagtt gattatctg gataaatca gatgggact 
      gtagtgttc cgaacttgc ccaagagag aaaaacaga 
      gtggtggtg caattgggg acgtgcgac ccacaaaga 
      gccttaaaa gttgccaaa atgatcgaa aatgatgta 
      gctgggatt gatataaac atgggttgt cccaaagaa 
      ttttcactg aaaggggga atgggggct gctttgtta 
      aaacagcca gagaaggcg aagtcaatt ttatccaca 
      cttacgcaa aatgtaaaa attccaatt acgtgcaaa 
      attcgtgtt tttgaaagt gctgaggaa accttagat 
      ttggtcaaa gaattggtg tcaacgggt atttcagct 
      attgcaatt catggaaga accaaagct gaaaggcct 
      caacatccc aacagaaac gcaacaatc aaatttatt 
      gcagagaac gtatcaatc ccggttgtt gccaatggt 
      gggtccaag gaaattgaa aaatatcaa gacataatt 
      aaatttaag gaagaatgt ggctgttca agtgtgatg 
      atcgcaagg gctgcagaa gccaattgt tcaattttt 
      cgtaaacag ggtttatta gatatcgag actgttata 
      gaacagtac ctcaaatat gccattgat tatgataac 
      tcgcccagt aatacaaaa tactgcata caaaacata 
      ctgaaagaa cttcaggaa acacccaga gggaagaaa 
      tttttagaa tgtcaaaca ctggaacaa atttgtgca 
      atatgggac ctagccgat tactgccga aaaaaacaa 
      ctgcagttc cactctcag ggaattttg agtcgccga 
      gaaataaca ccagatatg ttcagtccc aaagccaaa 
      aaagttaag actgaatta gacgatgac gtaatcgaa 
      atgaaatgt gcctttatt agggtaaat tatccagac 
      gatccagat ttgccgaaa tcaaaaata atagcatat 
      tgtggcaaa cacaagttt gatttgcct aagtaccag 
      accgtcaat gaagataaa ttatttcgc gcaattgcc 
      acattccaa gggaaaaaa tacagctca tcgtattgg 
      gaaaaaaat aaacgcttt gctgaacag ggggctgct 
      ttggtcctg gcctgttct ttaggttta gttacgaaa 
      gaggaatta attaaagat ggaagcatg ttggaataa
    mRNA sequence
    • >TC014107 RA
      acacatgac agtaatcct aacctcaaa aatgacaaa 
      aactactga aaaattaac ttacgacaa caaaatcat 
      attggcccc catggtacg tgttggtac tctacccat 
      gagattgtt gtcgctgga ccacggggc cgacattgt 
      ctacactga agaattaat tgactggaa atttttaaa 
      atcaataag acgggaaaa tgacgtgtt gggcacagt 
      tgattatct ggataaatc agatgggac tgtagtgtt 
      ccgaacttg cccaagaga gaaaaacag agtggtggt 
      gcaattggg gacgtgcga cccacaaag agccttaaa 
      agttgccaa aatgatcga aaatgatgt agctgggat 
      tgatataaa catgggttg tcccaaaga attttcact 
      gaaaggggg aatgggggc tgctttgtt aaaacagcc 
      agagaaggc gaagtcaat tttatccac acttacgca 
      aaatgtaaa aattccaat tacgtgcaa aattcgtgt 
      ttttgaaag tgctgagga aaccttaga tttggtcaa 
      agaattggt gtcaacggg tatttcagc tattgcaat 
      tcatggaag aaccaaagc tgaaaggcc tcaacatcc 
      caacagaaa cgcaacaat caaatttat tgcagagaa 
      cgtatcaat cccggttgt tgccaatgg tgggtccaa 
      ggaaattga aaaatatca agacataat taaatttaa 
      ggaagaatg tggctgttc aagtgtgat gatcgcaag 
      ggctgcaga agccaattg ttcaatttt tcgtaaaca 
      gggtttatt agatatcga gactgttat agaacagta 
      cctcaaata tgccattga ttatgataa ctcgcccag 
      taatacaaa atactgcat acaaaacat actgaaaga 
      acttcagga aacacccag agggaagaa atttttaga 
      atgtcaaac actggaaca aatttgtgc aatatggga 
      cctagccga ttactgccg aaaaaaaca actgcagtt 
      ccactctca gggaatttt gagtcgccg agaaataac 
      accagatat gttcagtcc caaagccaa aaaagttaa 
      gactgaatt agacgatga cgtaatcga aatgaaatg 
      tgcctttat tagggtaaa ttatccaga cgatccaga 
      tttgccgaa atcaaaaat aatagcata ttgtggcaa 
      acacaagtt tgatttgcc taagtacca gaccgtcaa 
      tgaagataa attatttcg cgcaattgc cacattcca 
      agggaaaaa atacagctc atcgtattg ggaaaaaaa 
      taaacgctt tgctgaaca gggggctgc tttggtcct 
      ggcctgttc tttaggttt agttacgaa agaggaatt 
      aattaaaga tggaagcat gttggaata aagccttat 
      aattattaa cgagttttt ttgtttttc cctgttata 
      caacgaaaa atcttttaa caagtggtc agagtaatt 
      ttcttatga ttgaaattc tcgttctca tagtcggtt 
      tcggcgact aacgtaaat ctgtaccga ataaaaata 
      aatatgaaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY