Status
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Gene information
General information
TC013721 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5398907..5402928
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013721
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10001

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013721 RA
      MRIGIHSGS VLCGVLGLR KWQFDIFSY DVRLANHME 
      SGGIPGRVH ISEATYQCL NGAYEVEPG NGQERDCYL 
      REHDVTTYL IKQVEPMKP RRRLASRPS IFSNKLWPD 
      DENGGSAMS SPKTPSTPA SPPPQTSIE RSDSGIQQS 
      TVEEENTTD WTPEIPFEN LHHALSGDL DEDIDSINY 
      PYNKQKYDL AESRRKTGT FRSTIEQVD DIIDHSIEI 
      ESNKRMRSA NVNAWTLRF IDKDMEYQF SQLREDMFK 
      SNMLCCFII WLFIVVCQV VAFKHLDDV VIGLLTVIT 
      VILTSTSVL VMAEEFEQL PEYLQKISY TLVHNRRSR 
      TAFICGVII IMAMTSSAS LILREQDND NCHPPSTSS 
      LNSLSTPNP PMANTSDKT VELLLNLLT SGRNVTIMT 
      LDSPRQNCS DNNCLRNLL KSIEIDELV KEAENISLL 
      NSEMTAVIN SINQKLKVL NQTLLSIKH ETVRKKRSL 
      GEDSNRDSN STNDAETDS KPRCPHPEY IVFTWILCL 
      IALATALKL YYLIKLFLA IVMLTVYIV LILEPYEEV 
      FKNACSGNT IPLPFQMTI LLCVFFLMV AYHARLVEV 
      TSRLDFLWK QQAERELND MEETRHTNT QLLKNILPD 
      HVAQHFLSN ERNSEELYS QSREEVGVL FASIPNFTE 
      FYSEDINKG LECIRLLNE IIADFDELL DEERFSMIE 
      KIKTVSATA TYMAASGLN PTHKDALNT DSPEHLCAL 
      IDFAIAMRQ KLDEINKES FNNFGLRVG VSCGSLVCG 
      VIGARKPVF DIWGNTVNE ASRMDSTGV IGQIQVPKY 
      TAQLLGVRG YEVKKRGII EVKGKGQME TYFVMGRQQ 
      GRPPSFQRQ PSHYSTLAA VVYAMAQTR RKHTGHTPG 
      SSVLGRART QQKIDVGKK IINYSSMRI PHRTPAYPI 
      RRNTTRANQ RNMHARSQP NMRQLGSGS HLENIKSYK 
      SIDKDITVN NISKMMVSQ SAPHTPVSA PNHTEGPIF 
      KSVPRLLSE PMVGYRSKS SSPATKTSK LERNSPQLG 
      DSFKVRSLG PHVPVHTSP KHLKKDKFF LKSPLIKRD 
      TKIQLERGG SKEKPERIN SLDGTHV
    CDS sequence
    • >TC013721 RA
      atgaggata ggaattcac tccggatcg gtcctttgt 
      ggggtcctt ggactgcgc aaatggcag tttgacata 
      ttttcatac gatgtccga ttggccaac cacatggaa 
      tccggcggg attccgggc cgtgtccac atatccgaa 
      gcgacttat caatgcctg aacggggct tacgaagtg 
      gagcctggg aacggccaa gagcgcgac tgttacctc 
      cgcgaacac gacgtcaca acctattta ataaaacaa 
      gtagagccg atgaaacct cgccggaga cttgcttcc 
      cgccctagc attttctct aacaaattg tggcccgac 
      gacgaaaac ggcgggagt gcaatgagc tctccgaag 
      acgccgtcc actccagca tcgcccccg ccccaaact 
      tccatcgag cggagcgac tccgggatc cagcaaagc 
      acagtggag gaggagaac acgacggac tggaccccg 
      gaaatccca tttgaaaat ttgcatcat gccctttcc 
      ggcgacttg gacgaggat atcgactcg attaattat 
      ccttacaat aagcaaaag tacgatttg gcggagtcc 
      aggcgcaaa accggcact ttccgctcg actatcgag 
      caagtggac gatatcatc gaccacagc atcgagatc 
      gagagcaac aaacgcatg cgaagcgcg aatgtgaac 
      gcctggact ctcaggttc atcgacaag gacatggag 
      taccaattc agccaactc cgggaggac atgttcaag 
      tcaaacatg ctgtgttgt ttcataatt tggctgttt 
      atcgttgtg tgccaagtc gttgctttc aagcatttg 
      gacgatgtt gttatcggt ttgttaacc gtcatcact 
      gttatttta acatcgacg agtgtgcta gtgatggca 
      gaggagttc gagcaactg cctgagtac ctccagaaa 
      atctcgtac actttggtg cacaacaga cgcagcaga 
      acggcgttc atctgcggt gttataatt ataatggca 
      atgacgagc tcggcgagt ttgatttta agagagcaa 
      gacaacgat aactgccat ccaccttca acctcttcc 
      ctaaatagt ttatccacc ccaaaccca ccaatggca 
      aacacgtcg gataaaacc gtcgaactg cttttgaat 
      ttgttaacc agtggacga aatgtaacc atcatgact 
      ttagattcg ccgagacaa aactgttcc gataacaat 
      tgtttgaga aatttactc aaatcgatc gaaatcgac 
      gaactggtc aaagaagcc gaaaatatc tcacttctc 
      aacagcgaa atgacggcc gttatcaac agcatcaat 
      caaaaactc aaagttctg aaccaaaca ctgctaagt 
      attaagcac gaaacggtt cgaaaaaaa cgctcactt 
      ggtgaggat tcgaaccga gattctaat agtacaaat 
      gatgccgaa acggactca aaaccgagg tgtccacac 
      ccagaatac atcgttttc acttggatt ctgtgtctc 
      atagcgctt gccacagcg ctgaaactc tactatttg 
      atcaaatta tttctagca atagtcatg ttgacggtt 
      tacatcgta ctcatcctg gaaccctac gaagaagtc 
      ttcaaaaac gcctgcagc ggcaacacg attccgctg 
      cccttccaa atgacaatc ctcctctgt gtttttttc 
      ttgatggtg gcttaccac gcaagattg gtcgaagtg 
      acttcgcgt ttggacttc ttgtggaag cagcaagct 
      gaacgtgaa ctgaacgat atggaggag acgcgacac 
      acaaacacc cagttactg aagaacatt cttcctgac 
      cacgtcgct cagcatttt ttgagcaac gaacgcaac 
      agcgaagag ttgtattcg caatcgagg gaggaagtt 
      ggtgtcctt ttcgcgagc attcccaac tttaccgag 
      ttctattcg gaggatatc aacaaaggg ctggagtgt 
      ataaggctg ctgaatgag attatcgcc gattttgac 
      gagttgttg gacgaggag cggtttagt atgattgag 
      aaaattaaa acagtgagc gcgacggcc acttacatg 
      gccgcgtct gggctcaat cccacccat aaagatgcc 
      ctgaacacg gacagtcct gagcattta tgtgccctt 
      atcgacttt gccatcgca atgcgtcag aagctggac 
      gagattaac aaagaatcg tttaataat tttggactt 
      cgggtgggg gtttcatgc gggagtctt gtgtgtggc 
      gtcattggg gcaagaaag cctgtgttt gatatttgg 
      ggtaataca gtcaatgag gcgtctcgg atggactcg 
      acaggggtc ataggccaa atccaagtc cccaaatac 
      acagcacag ctacttggg gtccgaggc tacgaggtg 
      aaaaagcga gggataatc gaagttaag ggcaaaggt 
      cagatggag acctacttc gtgatgggg cggcaacaa 
      gggcgcccc cccagcttc cagcgccaa ccgagccat 
      tacagcacc ttggcggct gtggtctac gccatggcc 
      cagactcgg cgcaaacac actggacac actcccgga 
      agttccgtg ctggggcgc gcccgcacc cagcaaaaa 
      atcgacgtg ggcaagaag ataatcaac tacagttcg 
      atgcggata ccccatcgg accccagcg taccccatc 
      cgccgcaac acgacgcgg gccaaccag agaaacatg 
      cacgcgcgg tcccaaccc aacatgcgc cagctgggc 
      tccgggagc cacctggag aacatcaaa tcgtacaag 
      tcgatcgat aaagacatc accgtgaac aacatcagc 
      aaaatgatg gtgtcccaa tccgccccc cacaccccc 
      gtgtccgcc cccaaccac accgaaggc cccatcttc 
      aagtcagtc cccagactc ctgtccgag cccatggtg 
      ggctaccgg tcgaagtcg tcatcgccg gcgaccaag 
      acgagcaaa ctggagcgg aactcgccg cagttgggg 
      gacagcttc aaggtgcgc tcgctgggc ccccacgtg 
      ccggtgcac acctcgccg aagcatctg aagaaggac 
      aagtttttc cttaaatcg cctctgatt aaaagggac 
      acgaaaatt cagcttgag agagggggc agtaaggag 
      aaacctgaa aggatcaac tcgttggat ggcactcac 
      gtttaa
    mRNA sequence
    • >TC013721 RA
      taaacgaaa aaaactcca aaattttct accacgtct 
      agattttaa gtttaaaga tgatttatt tcaaaattt 
      atgttttaa ggttgactt aaatatgag gataggaat 
      tcactccgg atcggtcct ttgtggggt ccttggact 
      gcgcaaatg gcagtttga catattttc atacgatgt 
      ccgattggc caaccacat ggaatccgg cgggattcc 
      gggccgtgt ccacatatc cgaagcgac ttatcaatg 
      cctgaacgg ggcttacga agtggagcc tgggaacgg 
      ccaagagcg cgactgtta cctccgcga acacgacgt 
      cacaaccta tttaataaa acaagtaga gccgatgaa 
      acctcgccg gagacttgc ttcccgccc tagcatttt 
      ctctaacaa attgtggcc cgacgacga aaacggcgg 
      gagtgcaat gagctctcc gaagacgcc gtccactcc 
      agcatcgcc cccgcccca aacttccat cgagcggag 
      cgactccgg gatccagca aagcacagt ggaggagga 
      gaacacgac ggactggac cccggaaat cccatttga 
      aaatttgca tcatgccct ttccggcga cttggacga 
      ggatatcga ctcgattaa ttatcctta caataagca 
      aaagtacga tttggcgga gtccaggcg caaaaccgg 
      cactttccg ctcgactat cgagcaagt ggacgatat 
      catcgacca cagcatcga gatcgagag caacaaacg 
      catgcgaag cgcgaatgt gaacgcctg gactctcag 
      gttcatcga caaggacat ggagtacca attcagcca 
      actccggga ggacatgtt caagtcaaa catgctgtg 
      ttgtttcat aatttggct gtttatcgt tgtgtgcca 
      agtcgttgc tttcaagca tttggacga tgttgttat 
      cggtttgtt aaccgtcat cactgttat tttaacatc 
      gacgagtgt gctagtgat ggcagagga gttcgagca 
      actgcctga gtacctcca gaaaatctc gtacacttt 
      ggtgcacaa cagacgcag cagaacggc gttcatctg 
      cggtgttat aattataat ggcaatgac gagctcggc 
      gagtttgat tttaagaga gcaagacaa cgataactg 
      ccatccacc ttcaacctc ttccctaaa tagtttatc 
      caccccaaa cccaccaat ggcaaacac gtcggataa 
      aaccgtcga actgctttt gaatttgtt aaccagtgg 
      acgaaatgt aaccatcat gactttaga ttcgccgag 
      acaaaactg ttccgataa caattgttt gagaaattt 
      actcaaatc gatcgaaat cgacgaact ggtcaaaga 
      agccgaaaa tatctcact tctcaacag cgaaatgac 
      ggccgttat caacagcat caatcaaaa actcaaagt 
      tctgaacca aacactgct aagtattaa gcacgaaac 
      ggttcgaaa aaaacgctc acttggtga ggattcgaa 
      ccgagattc taatagtac aaatgatgc cgaaacgga 
      ctcaaaacc gaggtgtcc acacccaga atacatcgt 
      tttcacttg gattctgtg tctcatagc gcttgccac 
      agcgctgaa actctacta tttgatcaa attatttct 
      agcaatagt catgttgac ggtttacat cgtactcat 
      cctggaacc ctacgaaga agtcttcaa aaacgcctg 
      cagcggcaa cacgattcc gctgccctt ccaaatgac 
      aatcctcct ctgtgtttt tttcttgat ggtggctta 
      ccacgcaag attggtcga agtgacttc gcgtttgga 
      cttcttgtg gaagcagca agctgaacg tgaactgaa 
      cgatatgga ggagacgcg acacacaaa cacccagtt 
      actgaagaa cattcttcc tgaccacgt cgctcagca 
      ttttttgag caacgaacg caacagcga agagttgta 
      ttcgcaatc gagggagga agttggtgt ccttttcgc 
      gagcattcc caactttac cgagttcta ttcggagga 
      tatcaacaa agggctgga gtgtataag gctgctgaa 
      tgagattat cgccgattt tgacgagtt gttggacga 
      ggagcggtt tagtatgat tgagaaaat taaaacagt 
      gagcgcgac ggccactta catggccgc gtctgggct 
      caatcccac ccataaaga tgccctgaa cacggacag 
      tcctgagca tttatgtgc ccttatcga ctttgccat 
      cgcaatgcg tcagaagct ggacgagat taacaaaga 
      atcgtttaa taattttgg acttcgggt gggggtttc 
      atgcgggag tcttgtgtg tggcgtcat tggggcaag 
      aaagcctgt gtttgatat ttggggtaa tacagtcaa 
      tgaggcgtc tcggatgga ctcgacagg ggtcatagg 
      ccaaatcca agtccccaa atacacagc acagctact 
      tggggtccg aggctacga ggtgaaaaa gcgagggat 
      aatcgaagt taagggcaa aggtcagat ggagaccta 
      cttcgtgat ggggcggca acaagggcg cccccccag 
      cttccagcg ccaaccgag ccattacag caccttggc 
      ggctgtggt ctacgccat ggcccagac tcggcgcaa 
      acacactgg acacactcc cggaagttc cgtgctggg 
      gcgcgcccg cacccagca aaaaatcga cgtgggcaa 
      gaagataat caactacag ttcgatgcg gatacccca 
      tcggacccc agcgtaccc catccgccg caacacgac 
      gcgggccaa ccagagaaa catgcacgc gcggtccca 
      acccaacat gcgccagct gggctccgg gagccacct 
      ggagaacat caaatcgta caagtcgat cgataaaga 
      catcaccgt gaacaacat cagcaaaat gatggtgtc 
      ccaatccgc cccccacac ccccgtgtc cgcccccaa 
      ccacaccga aggccccat cttcaagtc agtccccag 
      actcctgtc cgagcccat ggtgggcta ccggtcgaa 
      gtcgtcatc gccggcgac caagacgag caaactgga 
      gcggaactc gccgcagtt gggggacag cttcaaggt 
      gcgctcgct gggccccca cgtgccggt gcacacctc 
      gccgaagca tctgaagaa ggacaagtt tttccttaa 
      atcgcctct gattaaaag ggacacgaa aattcagct 
      tgagagagg gggcagtaa ggagaaacc tgaaaggat 
      caactcgtt ggatggcac tcacgttta atcgatgtt 
      tttttggtg tcgtttcat tgtgatagg ggggtttga 
      gtaaatcta gtcattttt ttattttcg ggaccaatt 
      tatctctgg agggtctgc cgcttttat gttgtaaat 
      aaatgttgt tggaacgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY