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Gene information
General information
TC013726 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5261237..5262648
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013726
    iBeetle screen iB_10000

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 2
    previtellogenic egg chamber potentially number increased - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013726 RA
      MGSQPLEFI RQVLLLVFT PHCYDNYFI HLNFFDGPC 
      FSSTLSKCL GLGIIMGSL LVKLPQIIK IYKNKSGEG 
      ISLLSVTLD LTAITIYAS YSFLKQFPF SAWGDAAFL 
      AIQTVLVGV LVLHYGGSS SKALLYLVA YLLVNFILM 
      SGLTPISVL WTLQGFNIF IVVSGKLTQ AYSNYKNGT 
      TGQLSAATL IMLFMGSLA RIFTSIQET GDKMVILTY 
      IASTLANGV LVAQLLYYW NVTPQKEKA H
    CDS sequence
    • >TC013726 RA
      atgggctca caaccgctc gaattcatt cgacaagtc 
      cttttattg gtttttacg ccccactgt tacgataat 
      tatttcatt catttgaat tttttcgat gggccgtgt 
      ttttcttca actttgagc aaatgccta ggtctaggc 
      ataattatg ggctcactt ttggtaaaa ctgccccaa 
      atcatcaaa atttacaag aataaaagc ggggagggc 
      atcagtttg ctcagtgtt actttggac ttgactgca 
      ataacgatt tacgcatct tatagtttc ctcaaacag 
      ttcccgttc agtgcgtgg ggcgatgcc gcctttctt 
      gcgatacag acagtcctt gttggggtt ttggtgctc 
      cattatggg ggttctagc tcaaaagca cttctatat 
      ttagttgct tatttgtta gtcaatttc attctaatg 
      agtggcttg acccctatc agtgtctta tggacattg 
      caaggtttt aacattttc attgttgtt agcgggaag 
      ttgacacaa gcttatagt aattacaaa aatggaact 
      acaggtcaa ttgtcagcg gctactttg attatgtta 
      tttatgggg tctctagcc aggattttc acctcgatt 
      caggagacg ggggacaag atggtcatt ctgacctat 
      attgctagc actcttgcc aatggggtg ctggtagcg 
      cagttgctc tattactgg aacgtaaca ccacaaaaa 
      gaaaaggct cactag
    mRNA sequence
    • >TC013726 RA
      agccgattc tcgcggaaa ataaacatg atgttttag 
      gggtttcgg ctggggagc aggtgccgc ctggaagaa 
      aaaccttga ttcggaatc cgcatccca aaccttacg 
      tctgctttt tggcgaaat gtatatttc agtaactga 
      gtcttcgtt tgatggggg gacactcat cttgacgtt 
      tgaattttc gcgctactg tcaccaaac tatcaattc 
      ctacgttat gaattttgt gcgttatgg atttgtcat 
      taatttgaa attaagtca aagtcataa attaagtgg 
      ctttttatc aaacacagt agcaaacga tgagaacga 
      cgtttattt aatttaaat atctcagtt cttatcgcc 
      tcaaatcac agataataa cactagtgg cgtgacata 
      acctcacca cggtccttg acgtgtcat cattttaaa 
      gaagacaca aaaaatggg ctcacaacc gctcgaatt 
      cattcgaca agtcctttt attggtttt tacgcccca 
      ctgttacga taattattt cattcattt gaatttttt 
      cgatgggcc gtgtttttc ttcaacttt gagcaaatg 
      cctaggtct aggcataat tatgggctc acttttggt 
      aaaactgcc ccaaatcat caaaattta caagaataa 
      aagcgggga gggcatcag tttgctcag tgttacttt 
      ggacttgac tgcaataac gatttacgc atcttatag 
      tttcctcaa acagttccc gttcagtgc gtggggcga 
      tgccgcctt tcttgcgat acagacagt ccttgttgg 
      ggttttggt gctccatta tgggggttc tagctcaaa 
      agcacttct atatttagt tgcttattt gttagtcaa 
      tttcattct aatgagtgg cttgacccc tatcagtgt 
      cttatggac attgcaagg ttttaacat tttcattgt 
      tgttagcgg gaagttgac acaagctta tagtaatta 
      caaaaatgg aactacagg tcaattgtc agcggctac 
      tttgattat gttatttat ggggtctct agccaggat 
      tttcacctc gattcagga gacggggga caagatggt 
      cattctgac ctatattgc tagcactct tgccaatgg 
      ggtgctggt agcgcagtt gctctatta ctggaacgt 
      aacaccaca aaaagaaaa ggctcacta gggatttag 
      ggattaaaa aaatgtgca taagtttca atttttagt 
      gtaaaatat caccagttg atgagactg ttaaattcg 
      tgtacaaat gttgcagtt tatcattcc ctgttaacg 
      tgtaagtct ttgattatt aaacgactg tttgggaaa 
      aataaaaat ggtaattaa acagaagtg ggtagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY