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Gene information
General information
TC014048 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4889478..4894542
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014048
    iBeetle screen iB_09997

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014048 RA
      MHKRGQVNK KSLEEEQQA IGAIYKQYI DTFQEHSDI 
      PRKAFVKSD TLYAEGKEN SEKGQLYNP KPIIKLKKE 
      PTNAHQQIS FNINKRAKK KSNLKALGD ELKMLQHER 
      QVKEIKLVV PKLEPPPLV THESEYTNS TNLFVSNLN 
      PQVTENHLI QLFGTYGPL ASVKIMWPR NETSSRSAN 
      CGFVAFMSR SDAERAMNG LKNHEEMRI NWGKSVKIP 
      SYPVHIPPE LYKLYSPPP PSGLPFNAQ TNVAKSELK 
      DLTQTVVKV TIPFDRNLL MIIHRMIEF VIREGPEFE 
      ALIMDMESG NPEFKFLSD FQDPAHTYY RWKLYSMLN 
      GDPKNSWSM KPFRMYENG SVWIPPTAP NYREGMPPE 
      LIKNPKTEA NLSESQTAR LVSLIKHLT TSKSSVSEA 
      MVFCVSHSN ALGDVLQVL VDSFKNVAT NPVKKLARL 
      YLLTDVLYN CKVRRIRYC EEFTPEVCT EIFNQLHST 
      YKELHHAQD KVCFKIKVF VVLRAWLFH RFYDTKFVT 
      KLENVLLTG NENGEDDSF HDEPLDGAN LLKRSYHTA 
      TTDCLIVEK KKQFVDMFN
    CDS sequence
    • >TC014048 RA
      atgcataaa cgcggccaa gtgaacaaa aaatcgctc 
      gaagaggag cagcaagcg attggggcg atctacaag 
      cagtatatt gacactttc caggagcat tcagacatc 
      ccgaggaaa gccttcgtc aagtcggat actctgtac 
      gccgaggga aaagaaaat tcggaaaag ggccaattg 
      tacaacccc aaaccaatc attaaactg aagaaggaa 
      ccaacgaat gctcatcag caaatttcg tttaatatc 
      aataaacgc gccaagaaa aaatcaaat ctgaaagcc 
      ctcggagac gaactcaag atgttgcag cacgaacgc 
      caagtcaaa gagattaaa ttggtggtg cctaagttg 
      gaacctcca ccgttggtg actcatgaa agtgaatac 
      acgaattcg accaatctt tttgtgtcg aatttgaat 
      ccgcaagtg actgagaat cacctcatt cagctcttt 
      gggacctac gggcccttg gccagtgtt aagattatg 
      tggcctcgt aacgagacg agttcgaga agtgcgaac 
      tgcggtttt gtggctttt atgagccgt tcggacgcc 
      gaacgcgca atgaacgga ttaaaaaac cacgaagaa 
      atgagaatc aattggggc aaatcagtc aaaatcccc 
      tcgtatccc gtccacatc ccccctgaa ctgtacaaa 
      ctgtattca cctccacct ccttcggga ttacccttt 
      aacgcgcaa acgaacgtc gcgaagagt gaattgaaa 
      gacttgacg caaaccgtg gtcaaagtc acgataccc 
      ttcgaccga aaccttcta atgatcatc caccgaatg 
      atcgagttt gtgataagg gaagggccg gagtttgaa 
      gccttgata atggacatg gagtcgggg aatcccgaa 
      tttaaattc ctgtcagat tttcaagac ccagcccac 
      acctactac cgctggaag ttgtattcg atgttgaac 
      ggtgacccg aaaaattcc tggtcgatg aaaccgttc 
      cgaatgtat gagaacgga tcagtttgg atccctccg 
      actgcgccg aattatcgc gaaggcatg cctccagaa 
      ttgatcaaa aaccccaaa actgaagcg aatttgtcc 
      gaatcgcaa acggcgcgt ttggtttct ttgatcaaa 
      catttgacg acgagtaaa agctcggtt tccgaagcc 
      atggtgttc tgtgtgagc cactcgaac gctttaggt 
      gatgtcttg caagttttg gtcgactcg ttcaaaaat 
      gtggccacg aatccggtg aaaaaactc gcccgactt 
      tacttactg accgatgtt ttgtataat tgcaaagtg 
      aggcggatt cgttattgc gaggaattc actccggaa 
      gtttgcacg gaaattttt aaccagctc cacagcact 
      tacaaggaa ttacatcac gcacaggat aaagtctgt 
      tttaaaatt aaagttttt gtcgtgttg agggcttgg 
      ctgtttcat cgcttttac gacaccaag tttgtcaca 
      aaactggaa aatgtgctc ttgaccgga aacgaaaac 
      ggcgaagat gattcgttt catgatgaa ccactggat 
      ggtgcgaat ttgttgaaa aggtcgtac catacggcc 
      acgactgat tgtttgatt gttgagaaa aagaaacag 
      tttgtcgat atgtttaat taa
    mRNA sequence
    • >TC014048 RA
      tttcgaacc aaataatta ataattaac ttttcgttc 
      caaaaagtg tgtaaaaat gcataaacg cggccaagt 
      gaacaaaaa atcgctcga agaggagca gcaagcgat 
      tggggcgat ctacaagca gtatattga cactttcca 
      ggagcattc agacatccc gaggaaagc cttcgtcaa 
      gtcggatac tctgtacgc cgagggaaa agaaaattc 
      ggaaaaggg ccaattgta caaccccaa accaatcat 
      taaactgaa gaaggaacc aacgaatgc tcatcagca 
      aatttcgtt taatatcaa taaacgcgc caagaaaaa 
      atcaaatct gaaagccct cggagacga actcaagat 
      gttgcagca cgaacgcca agtcaaaga gattaaatt 
      ggtggtgcc taagttgga acctccacc gttggtgac 
      tcatgaaag tgaatacac gaattcgac caatctttt 
      tgtgtcgaa tttgaatcc gcaagtgac tgagaatca 
      cctcattca gctctttgg gacctacgg gcccttggc 
      cagtgttaa gattatgtg gcctcgtaa cgagacgag 
      ttcgagaag tgcgaactg cggttttgt ggcttttat 
      gagccgttc ggacgccga acgcgcaat gaacggatt 
      aaaaaacca cgaagaaat gagaatcaa ttggggcaa 
      atcagtcaa aatcccctc gtatcccgt ccacatccc 
      ccctgaact gtacaaact gtattcacc tccacctcc 
      ttcgggatt accctttaa cgcgcaaac gaacgtcgc 
      gaagagtga attgaaaga cttgacgca aaccgtggt 
      caaagtcac gataccctt cgaccgaaa ccttctaat 
      gatcatcca ccgaatgat cgagtttgt gataaggga 
      agggccgga gtttgaagc cttgataat ggacatgga 
      gtcggggaa tcccgaatt taaattcct gtcagattt 
      tcaagaccc agcccacac ctactaccg ctggaagtt 
      gtattcgat gttgaacgg tgacccgaa aaattcctg 
      gtcgatgaa accgttccg aatgtatga gaacggatc 
      agtttggat ccctccgac tgcgccgaa ttatcgcga 
      aggcatgcc tccagaatt gatcaaaaa ccccaaaac 
      tgaagcgaa tttgtccga atcgcaaac ggcgcgttt 
      ggtttcttt gatcaaaca tttgacgac gagtaaaag 
      ctcggtttc cgaagccat ggtgttctg tgtgagcca 
      ctcgaacgc tttaggtga tgtcttgca agttttggt 
      cgactcgtt caaaaatgt ggccacgaa tccggtgaa 
      aaaactcgc ccgacttta cttactgac cgatgtttt 
      gtataattg caaagtgag gcggattcg ttattgcga 
      ggaattcac tccggaagt ttgcacgga aatttttaa 
      ccagctcca cagcactta caaggaatt acatcacgc 
      acaggataa agtctgttt taaaattaa agtttttgt 
      cgtgttgag ggcttggct gtttcatcg cttttacga 
      caccaagtt tgtcacaaa actggaaaa tgtgctctt 
      gaccggaaa cgaaaacgg cgaagatga ttcgtttca 
      tgatgaacc actggatgg tgcgaattt gttgaaaag 
      gtcgtacca tacggccac gactgattg tttgattgt 
      tgagaaaaa gaaacagtt tgtcgatat gtttaatta 
      aggaaaact tgtttgttg gttgattaa atgtgttct 
      tacctggcg atgagtgac atcgatatg ggaaatatt 
      cccatcgtt tttcctccc atgagatat tcggaagtg 
      gtgtcttgt ttgacttta gcaaagaag gcgaaataa 
      acaccaatc agcgacgat attagtaac attgcgccg 
      aaaacgatg taatcgaag atatcgaaa tatgtgaaa 
      atttcttcc gaactcatt ttggtttcg cacttgtaa 
      atcgaagaa catcactaa gctaaagtg acccattat 
      cctactacg taaatttag taagtataa tttagctac 
      aattggaaa attatgcgc cgtactata tacaggcta 
      ttctaaatt taaccgtca gccaaagac ggcacgaat 
      tacccgata a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY