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Gene information
General information
TC014045 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4865669..4869694
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014045
    iBeetle screen iB_09996

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Comment: no fat body present, but ovaries look OK
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014045 RA
      MSENFPDEC KSKLSKLPD STSYLAQSV DKSDLNFAY 
      DHMYASNTE NGEINTTQD ANCQSAESE VKFWKDWLI 
      LHLDLIQQQ SDEILNKER TILILQQEN EMLKERISC 
      MERGAPFHP NKFYNNHST SFLDEGLDM TQDSSPCDE 
      DNKNCLDLL DLNCENGTC PPLKLAKFE QNSDGESQT 
      GNPSIDNKF DSQVNYHKI SDKLKSESE IVTDCNSAS 
      AGFDFTVNS GEFDPMRNL RMSIRRKRL SNSSAVSNN 
      DSVLDDKRS FRKFKKRHK RILKDSQIL TTTDQYITE 
      AGRTNLRLS NVTDMQEIP NNTNLEVPR WRVKVYASC 
      YAMEGTENL DDEVYNKRH SRLENDERR RKRWDVQRI 
      REQRVIEKL KQRQERMGS GSRTDEQIE SVLSLWPTL 
      DNIKYLEVS DQLPVAAFG SPIPKIAPS EFSLPWLAN 
      PALITNKRG STKRTTVRK KNSKR
    CDS sequence
    • >TC014045 RA
      atgtccgaa aattttcca gacgaatgc aagtcgaaa 
      ttgtcaaaa ttaccggat tctacgagt tatttagcg 
      caaagtgtc gacaaatcc gacttgaat ttcgcctat 
      gatcatatg tacgcgtca aacacggaa aatggcgaa 
      ataaacaca acccaagat gcgaactgt caaagcgcc 
      gaaagtgag gttaagttt tggaaagac tggctgatt 
      ctccatctc gacctcatc cagcaacaa agcgatgaa 
      attctaaac aaggagaga acaatcctc attctgcaa 
      caagagaat gaaatgctt aaggagcgc ataagttgt 
      atggaacgc ggggccccg ttccacccc aacaagttc 
      tacaataac cactccacg tcgtttctg gacgaaggg 
      ctagatatg actcaggat tcgagtccc tgcgacgaa 
      gacaacaaa aattgtttg gaccttttg gacttgaac 
      tgtgagaac ggcacctgt ccgccgctc aaacttgcc 
      aaattcgaa caaaacagc gacggcgaa tctcaaacc 
      ggaaatcct tccatagac aataagttt gattctcag 
      gttaactat cataaaatt agtgacaaa ttgaaaagt 
      gagagtgaa atcgtaaca gattgtaac agtgctagt 
      gccggcttt gactttacg gttaattcg ggggaattc 
      gacccaatg agaaattta agaatgagt attaggagg 
      aaacggtta agtaattcg tcagcggtc tcaaataac 
      gactcagtg ttagacgac aaaaggtcc tttcggaaa 
      ttcaaaaaa cggcacaaa cgtattctg aaagattct 
      caaattttg accacaacc gatcagtac attactgaa 
      gccggtcga acaaatttg aggctgtcg aacgttaca 
      gacatgcaa gaaattcca aacaatacg aatttggag 
      gtgccccgc tggagggtt aaagtgtac gctagttgt 
      tacgcaatg gaagggact gaaaattta gacgatgaa 
      gtctacaac aaaagacac agtcggttg gaaaacgac 
      gaacgtagg agaaagcga tgggacgtt caaaggata 
      agagaacag cgggtaata gaaaaattg aagcagaga 
      caggaaaga atgggttcg gggtctcgg acggatgaa 
      caaatcgag tccgtgctg tctctttgg cccacttta 
      gacaacatt aaatatctg gaagtctct gatcagctg 
      ccagtggcg gcctttggc tcacctatt cccaaaata 
      gcccccagt gaatttagt ttgccatgg ttggctaac 
      cctgcgcta atcacgaat aagagaggg tcaacaaaa 
      cgaacaaca gtgagaaag aaaaactca aagagataa
    mRNA sequence
    • >TC014045 RA
      ggtgaaaaa accataaac gcgtctttg tttacttgt 
      ttaccaaca atttattgg cgaatttgg ttaaatatt 
      gcccaattt cgaaactga ttcgccaat tttgaagca 
      ttacggtgt taattaacg aaggaggaa aaaacgtag 
      tgagtcatt ggcctctga tgcgttttt gtttcgagt 
      gacaaacaa cctaacctt aaaatgtga tttcaatcc 
      aaagtgttg cattattta tgtgcaatt tttgaaaaa 
      tagtgactg ttgtgtaac caagtggag ggacttgtg 
      taattgtgt tacaattaa cgcttgtaa ttgtgacaa 
      aaacgatgc aaaagtgtg acttgatgt tcggtttgt 
      aattgataa ttatgcaaa taacgccac agagtctcg 
      cgtaatttc gataatgtt tatgtaatt aattatgcc 
      aataagtcg caacaatga ccaaaacat aaaacgact 
      ttgcgcgaa gatgtccga aaattttcc agacgaatg 
      caagtcgaa attgtcaaa attaccgga ttctacgag 
      ttatttagc gcaaagtgt cgacaaatc cgacttgaa 
      tttcgccta tgatcatat gtacgcgtc aaacacgga 
      aaatggcga aataaacac aacccaaga tgcgaactg 
      tcaaagcgc cgaaagtga ggttaagtt ttggaaaga 
      ctggctgat tctccatct cgacctcat ccagcaaca 
      aagcgatga aattctaaa caaggagag aacaatcct 
      cattctgca acaagagaa tgaaatgct taaggagcg 
      cataagttg tatggaacg cggggcccc gttccaccc 
      caacaagtt ctacaataa ccactccac gtcgtttct 
      ggacgaagg gctagatat gactcagga ttcgagtcc 
      ctgcgacga agacaacaa aaattgttt ggacctttt 
      ggacttgaa ctgtgagaa cggcacctg tccgccgct 
      caaacttgc caaattcga acaaaacag cgacggcga 
      atctcaaac cggaaatcc ttccataga caataagtt 
      tgattctca ggttaacta tcataaaat tagtgacaa 
      attgaaaag tgagagtga aatcgtaac agattgtaa 
      cagtgctag tgccggctt tgactttac ggttaattc 
      gggggaatt cgacccaat gagaaattt aagaatgag 
      tattaggag gaaacggtt aagtaattc gtcagcggt 
      ctcaaataa cgactcagt gttagacga caaaaggtc 
      ctttcggaa attcaaaaa acggcacaa acgtattct 
      gaaagattc tcaaatttt gaccacaac cgatcagta 
      cattactga agccggtcg aacaaattt gaggctgtc 
      gaacgttac agacatgca agaaattcc aaacaatac 
      gaatttgga ggtgccccg ctggagggt taaagtgta 
      cgctagttg ttacgcaat ggaagggac tgaaaattt 
      agacgatga agtctacaa caaaagaca cagtcggtt 
      ggaaaacga cgaacgtag gagaaagcg atgggacgt 
      tcaaaggat aagagaaca gcgggtaat agaaaaatt 
      gaagcagag acaggaaag aatgggttc ggggtctcg 
      gacggatga acaaatcga gtccgtgct gtctctttg 
      gcccacttt agacaacat taaatatct ggaagtctc 
      tgatcagct gccagtggc ggcctttgg ctcacctat 
      tcccaaaat agcccccag tgaatttag tttgccatg 
      gttggctaa ccctgcgct aatcacgaa taagagagg 
      gtcaacaaa acgaacaac agtgagaaa gaaaaactc 
      aaagagata agtattatg tcaacaacc gaaataaaa 
      ttttagtca agcaccact gcctgatgt ttttgtaaa 
      taaaaataa attatgaag tggtggaat gaacgaaaa 
      tgtgatttt atgaataca tacaggtta tttgtaaat 
      tgaaacaat ctcattaca tgacagttt gtaaagtat 
      ttatttagg ttgtaagct tacttttgg agtagatta 
      ctaggcgcc gtaatacca atttaggac tccatatta 
      gggttttag gttgcgaat tttaatttt taagtaatg 
      taatattgt gatatcgga attgttatg tccatagat 
      ttcctgata atgatatta atttacaaa taatgaatc 
      atatatttt tagttgaag cgcgttaaa taaataaat 
      gtattagtt gtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY