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Gene information
General information
TC013753 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4807812..4809126
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013753
    iBeetle screen iB_09994

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013753 RA
      MTFIAIIYQ IQIVRLQEK IFHLENNAN QNDRKQQSR 
      EELENDDLV VVYNRVPKT GSTSFIGVA YDLCKKNKF 
      HVLHVNITA NNHVLSLNN QHEFVHNVT TWKAMKPGI 
      YHGHFAFID FTKFGGPKP LFINILRKP LERFISYYY 
      FVRYGDNYR PYLVRRKHG NTMSFDECV EKNLPDCDP 
      NHMWLQIPF LCGHAANCW KPGNKWALT EAKKNLVNN 
      YLLVGVTDE INDFVAVLE QTLPRIFKG AFNHYLTSN 
      KSHLRQTVQ KDAPSPTTV KKIQESTVW QMENELYEF 
      ALDQFHFIK KHTLKDKLQ NVMYEKIRP KIN
    CDS sequence
    • >TC013753 RA
      atgaccttt attgcaata atttaccaa attcaaatc 
      gttcgactt caagaaaaa atcttccac cttgaaaac 
      aatgcaaat caaaacgac cgtaaacaa cagagtcga 
      gaagagtta gagaacgac gatttagta gttgtatac 
      aatcgagta ccaaagact ggatccaca agcttcata 
      ggcgtcgct tacgacttg tgtaaaaag aataaattc 
      catgtttta cacgtgaac ataactgcc aataatcac 
      gttttatcg ctaaacaat caacacgaa ttcgttcat 
      aacgtcacc acctggaag gcgatgaaa ccggggatc 
      taccacggc cactttgcc ttcatcgac ttcacgaaa 
      ttcgggggc cctaagccc ttgttcatc aacattctc 
      cgaaagccc ctagagcgg ttcatttcc tactactac 
      tttgtcaga tacggggac aactaccgg ccctatctg 
      gtgcgccgc aagcacggc aacaccatg tctttcgac 
      gaatgcgtt gagaaaaac ctccctgac tgcgacccc 
      aaccacatg tggctccag atccccttc ttgtgcggg 
      cacgccgcc aactgctgg aagcccggc aataagtgg 
      gccctaacc gaggcaaag aagaactta gtcaacaat 
      tacctcctc gtgggagta acggacgag atcaacgac 
      tttgttgcc gttttagag caaaccctt ccacggata 
      ttcaaaggc gcgttcaac cactacctc accagcaac 
      aagtcgcac ttgaggcag accgttcag aaggacgcg 
      ccttctccg accaccgtc aagaaaatc caagagagc 
      acagtgtgg cagatggaa aacgagctc tacgagttc 
      gccttggac caattccac tttattaaa aaacacact 
      cttaaagac aaactacag aacgtcatg tacgagaag 
      atacggccc aaaatcaac taa
    mRNA sequence
    • >TC013753 RA
      tcaggtgtt tgaagaaac cattcagtt caagcaatg 
      attaataaa acaaatagg atttgtttg tttcctgaa 
      taaaatact cgaaaccat gcatattat ttttcgata 
      aaaaaggtc aacaaaata cccaccggt gatgaatag 
      cgtaaggtt agggtacct tttaacaat aactgttat 
      gacctttat tgcaataat ttaccaaat tcaaatcgt 
      tcgacttca agaaaaaat cttccacct tgaaaacaa 
      tgcaaatca aaacgaccg taaacaaca gagtcgaga 
      agagttaga gaacgacga tttagtagt tgtatacaa 
      tcgagtacc aaagactgg atccacaag cttcatagg 
      cgtcgctta cgacttgtg taaaaagaa taaattcca 
      tgttttaca cgtgaacat aactgccaa taatcacgt 
      tttatcgct aaacaatca acacgaatt cgttcataa 
      cgtcaccac ctggaaggc gatgaaacc ggggatcta 
      ccacggcca ctttgcctt catcgactt cacgaaatt 
      cgggggccc taagccctt gttcatcaa cattctccg 
      aaagcccct agagcggtt catttccta ctactactt 
      tgtcagata cggggacaa ctaccggcc ctatctggt 
      gcgccgcaa gcacggcaa caccatgtc tttcgacga 
      atgcgttga gaaaaacct ccctgactg cgaccccaa 
      ccacatgtg gctccagat ccccttctt gtgcgggca 
      cgccgccaa ctgctggaa gcccggcaa taagtgggc 
      cctaaccga ggcaaagaa gaacttagt caacaatta 
      cctcctcgt gggagtaac ggacgagat caacgactt 
      tgttgccgt tttagagca aacccttcc acggatatt 
      caaaggcgc gttcaacca ctacctcac cagcaacaa 
      gtcgcactt gaggcagac cgttcagaa ggacgcgcc 
      ttctccgac caccgtcaa gaaaatcca agagagcac 
      agtgtggca gatggaaaa cgagctcta cgagttcgc 
      cttggacca attccactt tattaaaaa acacactct 
      taaagacaa actacagaa cgtcatgta cgagaagat 
      acggcccaa aatcaacta actcacgcc gcgagatat 
      tatttttct atgtgcacg acttaaacc gctgtctaa 
      tgtacttat ttataataa attgagatt atttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY