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Gene information
General information
TC014040 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4673932..4679121
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014040
    iBeetle screen iB_09992

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    yolk deposition irregular - Number of animals showing the phenotype: 4

    vitellogenic egg chamber potentiallynumber increased - Number of animals showing the phenotype: 2

    vitellogenic egg chamber late number decreased - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014040 RA
      MFARLVQFG VRSHNLSHF NYLKPTNCL KLAPITHCD 
      PTPVQQKRF YKNFGHKPK KTPTVTKLW YGLIFGLMF 
      LATVDYRWV RRTLFPPVK AQSETIGES SDVEQSESE 
      DESKKKKHR KEKIGFRDR KIIEYENRM RAYSTPDKI 
      FRYFATVRL ITPEGSEVY MTPDDFLRA MTPGMRQPD 
      GLGLDQYRR YDPKTVQQK LELTLDEDS IFYKLGSAG 
      LITFSDYIF LLTVLSTSR RHFEIAFRM FDLNGDGDV 
      DCEEFEKVA TLIRQQTSI GSRHRDHAN TGNTFKGVN 
      SALTTYFFG PNLKGKLTI EKFLDFQEK LQKEILSLE 
      FLRKNPDEN GNISEADFT ELLLAYAGY PQKKKSRML 
      KRVKKTFRD HGQGISKQD YLNFFHFLN NINDVDTAL 
      TFYHIAGAS IDQATLKHV AKTVAHVDL SDHVIHVVF 
      TIFDENLDG QLSNKEFIA VMKNRLLRG LEKPKDTGF 
      IKFMQSVLK CAKETRPAL LDI
    CDS sequence
    • >TC014040 RA
      atgtttgcg cgacttgtc caattcggt gtgaggtcg 
      cataacctc tcccatttc aactattta aaacccaca 
      aactgttta aaactagct cctataact cactgtgac 
      ccgactccg gtgcaacag aaacgtttc tataagaat 
      ttcggtcac aagcccaag aaaacccca accgtaaca 
      aaattgtgg tatggctta atttttgga cttatgttc 
      cttgcaaca gtcgattac agatgggtc cgccgcact 
      ttatttccc ccagtgaag gcacaaagc gaaacaatc 
      ggcgaaagc tccgacgtt gagcaatcc gaatctgaa 
      gatgagtcg aaaaagaag aaacaccgc aaggagaag 
      atcggtttc cgcgaccgc aaaataata gaatacgaa 
      aatcgtatg cgagcttat tccacacca gacaaaatt 
      ttccggtat ttcgctaca gtccggttg ataaccccc 
      gaaggcagc gaggtttac atgactccg gatgatttt 
      ctccgcgcg atgacgccc ggaatgaga cagcctgat 
      ggtctgggc ctcgatcag taccgacgc tacgaccct 
      aaaactgtc caacaaaaa ttggagctc actttggat 
      gaggacagc attttctac aagctaggg agtgcggga 
      cttatcacg ttctctgat tatatcttc cttttgacc 
      gtcttgtcg acttcgcga cgtcacttc gagattgcg 
      tttcgaatg tttgatctg aacggcgat ggtgacgta 
      gactgtgaa gagttcgaa aaagtggcc actttgatc 
      agacaacag acgagtata ggatcaaga catcgcgac 
      cacgccaac acaggaaac acattcaaa ggcgtgaat 
      tcggccctt acgacgtac ttcttcggc ccgaacctc 
      aagggcaaa ctcaccatc gagaagttc ctcgacttc 
      caggagaaa ctccagaag gaaattttg agtttggag 
      tttttgcgc aagaacccg gacgaaaac gggaacatc 
      agcgaagct gacttcacg gaactcctc ctcgcgtac 
      gccggctac ccccaaaag aagaaatcc cgcatgttg 
      aagcgagtc aaaaaaact tttagggat cacggccaa 
      ggcatttcc aagcaagac tatttgaat ttcttccat 
      tttttgaat aatattaac gatgttgac acagcttta 
      actttctac catatcgcg ggggcctcc atcgaccaa 
      gcaacgttg aagcacgtg gccaagacg gtggcccat 
      gtggatctc agtgaccat gtgattcat gtcgttttt 
      accattttc gatgagaat ttggatggg cagttgagc 
      aataaggag tttatcgcc gttatgaag aatcgctta 
      cttagaggg ctcgaaaaa ccaaaagat acgggtttt 
      atcaagttt atgcagtcg gtcttgaag tgtgccaaa 
      gagacaaga cccgccttg ctagatatt taa
    mRNA sequence
    • >TC014040 RA
      aaaaaaaag tcatcgaaa cgtcatatt attaaaaaa 
      taaaaataa taaaagttt tagccgatt ttgatgtga 
      aaatcgaca ttttctaaa taattgagc atgtaaatg 
      ttgtgtagt ggattggcg aaatgtttg cgcgacttg 
      tccaattcg gtgtgaggt cgcataacc tctcccatt 
      tcaactatt taaaaccca caaactgtt taaaactag 
      ctcctataa ctcactgtg acccgactc cggtgcaac 
      agaaacgtt tctataaga atttcggtc acaagccca 
      agaaaaccc caaccgtaa caaaattgt ggtatggct 
      taatttttg gacttatgt tccttgcaa cagtcgatt 
      acagatggg tccgccgca ctttatttc ccccagtga 
      aggcacaaa gcgaaacaa tcggcgaaa gctccgacg 
      ttgagcaat ccgaatctg aagatgagt cgaaaaaga 
      agaaacacc gcaaggaga agatcggtt tccgcgacc 
      gcaaaataa tagaatacg aaaatcgta tgcgagctt 
      attccacac cagacaaaa ttttccggt atttcgcta 
      cagtccggt tgataaccc ccgaaggca gcgaggttt 
      acatgactc cggatgatt ttctccgcg cgatgacgc 
      ccggaatga gacagcctg atggtctgg gcctcgatc 
      agtaccgac gctacgacc ctaaaactg tccaacaaa 
      aattggagc tcactttgg atgaggaca gcattttct 
      acaagctag ggagtgcgg gacttatca cgttctctg 
      attatatct tccttttga ccgtcttgt cgacttcgc 
      gacgtcact tcgagattg cgtttcgaa tgtttgatc 
      tgaacggcg atggtgacg tagactgtg aagagttcg 
      aaaaagtgg ccactttga tcagacaac agacgagta 
      taggatcaa gacatcgcg accacgcca acacaggaa 
      acacattca aaggcgtga attcggccc ttacgacgt 
      acttcttcg gcccgaacc tcaagggca aactcacca 
      tcgagaagt tcctcgact tccaggaga aactccaga 
      aggaaattt tgagtttgg agtttttgc gcaagaacc 
      cggacgaaa acgggaaca tcagcgaag ctgacttca 
      cggaactcc tcctcgcgt acgccggct acccccaaa 
      agaagaaat cccgcatgt tgaagcgag tcaaaaaaa 
      cttttaggg atcacggcc aaggcattt ccaagcaag 
      actatttga atttcttcc attttttga ataatatta 
      acgatgttg acacagctt taactttct accatatcg 
      cgggggcct ccatcgacc aagcaacgt tgaagcacg 
      tggccaaga cggtggccc atgtggatc tcagtgacc 
      atgtgattc atgtcgttt ttaccattt tcgatgaga 
      atttggatg ggcagttga gcaataagg agtttatcg 
      ccgttatga agaatcgct tacttagag ggctcgaaa 
      aaccaaaag atacgggtt ttatcaagt ttatgcagt 
      cggtcttga agtgtgcca aagagacaa gacccgcct 
      tgctagata tttaagccg aagcagact tattttacc 
      aaggattgt ttttaagac tgttagtgt tcatgcgaa 
      tagcaaata aagtatctc gaagttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY