Status
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Gene information
General information
TC014025 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4426650..4428183
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014025
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09988

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014025 RA
      MLKTTVISL LLIGVTRAE TISFKNVTL EWKTSTDRN 
      TTTIPSSAN LRDNIPKLQ TEISVVLSG LVPVLYPKS 
      VSGVPTLKS LTVSNVGLR EIKPGAIEN LPLLAHLIL 
      NRNELKVIE RGTFTNLNL TSVNLGDNE ISKLELGAF 
      KNLKIHHLT LSNNKISEI RNGTFENVS LQSLNLYGN 
      GLRKIDGPI TNLTNLVLS NNNLTEIDL HFPDLKYLD 
      LSFNSIQVL KPGDLKNLP ALDTLDLTA NTLHHVPEG 
      VFSQSKLVT LKLNYNQIA KIEPKSFDD MSQLSVLHI 
      DHNNLTQWD DKWLSNSPK LAYIFAGFN RIGALPGQA 
      FRNYPQMYS IDLQGNLIK EVSRESFAG LEKVVNFSL 
      RDNLIVDLD PEWLANATV GSVDLSGNK LGCVEGNLE 
      EAFRRVDFV HLEGNPWRE DCVEKIKTF LSQWPKIKR 
      IVSSVSQQP I
    CDS sequence
    • >TC014025 RA
      atgttaaaa acaactgtg atttcttta cttttgatt 
      ggtgtaaca cgagctgaa accatttct ttcaaaaat 
      gtaacactg gaatggaaa acaagcaca gatcgtaac 
      accacaacc attccatca tcagccaac cttcgagac 
      aacatccca aagcttcag accgagatt tcggtggtt 
      ttgagtggt ttagtgcct gttttatac ccaaaatca 
      gtgagtggt gtaccaact ttaaaatca ctaacagta 
      tcaaacgtc ggcttgaga gaaataaaa ccaggagcg 
      attgaaaat ttaccactt ctggcccat ttgatttta 
      aatcgcaat gagctcaaa gttattgaa cgaggaact 
      ttcacaaac ttaaacctg acaagtgtg aatttaggt 
      gacaatgaa atttccaaa ctcgaactt ggggctttc 
      aaaaacctc aaaattcac catctcaca ctcagtaac 
      aacaaaatt tctgagatt cgaaacggg acttttgaa 
      aacgtctca ctccaaagc ttgaatttg tacggaaat 
      gggctccga aaaatcgac ggaccaatc acaaactta 
      acaaatctg gtcctgagc aacaacaat ttgaccgaa 
      atcgatttg catttccct gatttaaaa tatctcgac 
      ttgagtttc aattccatt caagttcta aagcccgga 
      gacttgaaa aatcttccc gcactggac acgttggac 
      ctcaccgct aataccctt caccacgtc cctgaaggt 
      gtcttcagt cagtcgaaa ctggtaact ttgaaacta 
      aattacaac caaatcgcg aaaatcgaa cccaagtca 
      ttcgacgac atgtctcag ctcagtgtt ttgcacatc 
      gaccacaac aacttaacc caatgggat gacaaatgg 
      ttgagtaat tcccccaag ttggcctac attttcgcc 
      ggttttaac cgaattgga gctttgccg ggccaagcc 
      ttccggaat tatccccaa atgtacagc attgatttg 
      caaggaaat ttgattaaa gaagtttca cgggaaagt 
      ttcgcgggg ttggagaaa gtggtgaat tttagtttg 
      agggataat ttgattgtg gatttggac ccagaatgg 
      ctggctaat gcgacggtg ggaagtgtg gatttgagt 
      ggaaataag ctggggtgt gtggaggga aatttagag 
      gaggctttc agaagggtg gactttgtg catctggag 
      gggaatccc tggcgggag gattgtgtg gaaaagatt 
      aaaacattt ctttcccag tggccaaaa ataaaacgc 
      attgttagt tctgtgtca caacaacca atttga
    mRNA sequence
    • >TC014025 RA
      cccatcatc gataaagac taccagttt tcaagctac 
      tgttaaaat gttaaaaac aactgtgat ttctttact 
      tttgattgg tgtaacacg agctgaaac catttcttt 
      caaaaatgt aacactgga atggaaaac aagcacaga 
      tcgtaacac cacaaccat tccatcatc agccaacct 
      tcgagacaa catcccaaa gcttcagac cgagatttc 
      ggtggtttt gagtggttt agtgcctgt tttataccc 
      aaaatcagt gagtggtgt accaacttt aaaatcact 
      aacagtatc aaacgtcgg cttgagaga aataaaacc 
      aggagcgat tgaaaattt accacttct ggcccattt 
      gattttaaa tcgcaatga gctcaaagt tattgaacg 
      aggaacttt cacaaactt aaacctgac aagtgtgaa 
      tttaggtga caatgaaat ttccaaact cgaacttgg 
      ggctttcaa aaacctcaa aattcacca tctcacact 
      cagtaacaa caaaatttc tgagattcg aaacgggac 
      ttttgaaaa cgtctcact ccaaagctt gaatttgta 
      cggaaatgg gctccgaaa aatcgacgg accaatcac 
      aaacttaac aaatctggt cctgagcaa caacaattt 
      gaccgaaat cgatttgca tttccctga tttaaaata 
      tctcgactt gagtttcaa ttccattca agttctaaa 
      gcccggaga cttgaaaaa tcttcccgc actggacac 
      gttggacct caccgctaa tacccttca ccacgtccc 
      tgaaggtgt cttcagtca gtcgaaact ggtaacttt 
      gaaactaaa ttacaacca aatcgcgaa aatcgaacc 
      caagtcatt cgacgacat gtctcagct cagtgtttt 
      gcacatcga ccacaacaa cttaaccca atgggatga 
      caaatggtt gagtaattc ccccaagtt ggcctacat 
      tttcgccgg ttttaaccg aattggagc tttgccggg 
      ccaagcctt ccggaatta tccccaaat gtacagcat 
      tgatttgca aggaaattt gattaaaga agtttcacg 
      ggaaagttt cgcggggtt ggagaaagt ggtgaattt 
      tagtttgag ggataattt gattgtgga tttggaccc 
      agaatggct ggctaatgc gacggtggg aagtgtgga 
      tttgagtgg aaataagct ggggtgtgt ggagggaaa 
      tttagagga ggctttcag aagggtgga ctttgtgca 
      tctggaggg gaatccctg gcgggagga ttgtgtgga 
      aaagattaa aacatttct ttcccagtg gccaaaaat 
      aaaacgcat tgttagttc tgtgtcaca acaaccaat 
      ttgatctaa tattgatta aaaactttg tttgtagtt 
      ttattgttg tcccagagc gaaattatt aaatttacc 
      gcctgtttg aatggcgtt ctacacgat tgcgtccta 
      tgtttagta ggatatttt ctgttttga ttaagaatt 
      taattaaag tattaacgt aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY