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Gene information
General information
TC001633 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1238092..1240327
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001633
    iBeetle screen iB_09987

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva size thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: Only few larvae showed this phenotype; maybe background.
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Sometimes 1st thorax segment is present but 2nd and 3rd segments are absent.
    thorax constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Constriction occurs between thorax and abdomen segment
    thorax fragmented - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Fragmentation often separate thorax from abdomen.
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Sometimes 1st thorax segment is present but 2nd and 3rd segments are absent.
    abdomen constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Constriction occurs between thorax and abdomen segment
    abdomen fragmented - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Fragmentation often separate thorax from abdomen.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001633 RA
      METNVLSKW CRICSSEFN TGIFLFGEE ARRLFLHAK 
      IRRYLALTI SVEDKLPKM ICEDCYKKL DGFHRFATM 
      ALRNQDKMT KFVCTTTNN NHEQKNLEN KSLLHTYLT 
      KGIKEKETQ PESEITEME VRVDPMIIL QCSLETEDD 
      EEDNQSEEV SDGESHVQR ENLSESENC DKYRKPYLC 
      PSCPRSFTS ELALQNHTW NHSPPIKRI LNSHDNWLE 
      TEEKETEEN DASTNNDDE DNMEEFAMS RFSCPICGK 
      EISTKGNLK VHLETHRPK GKYGCDICG RIFKTQCNL 
      YRHKEYHGG IQYPCGVCG RVYPTNSTL RAHSITHSD 
      LRPHKCPLC DKTFKRNQD LKFHINQHT GARPYQCPY 
      CPKAFASSG NCFSHRKRM HPIEVERDR ERAAKIMR
    CDS sequence
    • >TC001633 RA
      atggaaaca aatgtactt tcaaaatgg tgtcggatt 
      tgttcttct gaatttaat accgggatt ttcctcttc 
      ggggaagag gctagaagg ctgtttctc catgctaaa 
      attagaaga tatctagca ctcacgata tcagttgag 
      gacaaacta ccaaaaatg atttgcgaa gactgctac 
      aagaagctc gacggcttt cacagattc gccacgatg 
      gcacttaga aaccaagac aaaatgacc aaattcgtc 
      tgcacaacc accaataac aaccacgag caaaaaaat 
      ttagaaaac aagtcactc ctccatacc tatctcact 
      aaagggatc aaagagaaa gaaacccag ccagaatca 
      gaaataacc gagatggag gtccgcgtg gaccccatg 
      atcatcctc cagtgctcc ctcgagacc gaagacgac 
      gaggaagac aaccagagc gaggaagtc tcagacggc 
      gaaagccac gtccagaga gagaacctc tcagagagc 
      gaaaactgt gacaaatat cgtaaacca tatctttgc 
      ccctcgtgt ccgaggagt ttcacgtcg gagctagcc 
      ctccagaac cacacctgg aaccactcc ccgcccatc 
      aaacgtatc ctcaacagc cacgacaac tggctggaa 
      accgaagaa aaagagact gaagaaaac gatgcttcc 
      acaaacaac gacgacgag gacaacatg gaggagttc 
      gccatgtcg cgcttctcg tgccccatc tgcggcaag 
      gagatttcg accaaaggc aacctcaag gttcacctc 
      gagacgcac agacccaag gggaaatac ggctgcgat 
      atttgcgga cgaatattc aagacccag tgcaacttg 
      taccgacac aaggagtac cacggcggg attcagtac 
      ccctgcggg gtatgcggc cgggtgtac cccacaaat 
      agcacttta agggcccat tctatcact cacagcgac 
      ctcaggccc cacaaatgc cccttgtgc gacaagacg 
      ttcaaaagg aaccaggat ttgaagttt catataaac 
      caacatact ggtgctagg ccgtatcaa tgtccctac 
      tgtcccaag gctttcgct agttctggc aactgcttc 
      tcgcaccgg aaacggatg catccgatt gaggttgag 
      cgggatagg gaacgggca gcgaaaatt atgcgataa
    mRNA sequence
    • >TC001633 RA
      ggctgtaag tgacgattc tggacggtt ttgagtgat 
      gtaaagccg ttttttacc actggcttg ggcgggatg 
      catgcatta ggtttttta aaaagcacg caacaggaa 
      ttgtatgaa ttgtacctt tagagtgtg tgcgcaatg 
      agccctgtt taagcacag tactttttt acgagacgc 
      tatgtattg ccgatctta cgtgcaaga aatatttaa 
      ttgatcggg tggcatttt tctcgcgaa tcttatcca 
      atgtgtcaa aatggaaac aaatgtact ttcaaaatg 
      gtgtcggat ttgttcttc tgaatttaa taccgggat 
      tttcctctt cggggaaga ggctagaag gctgtttct 
      ccatgctaa aattagaag atatctagc actcacgat 
      atcagttga ggacaaact accaaaaat gatttgcga 
      agactgcta caagaagct cgacggctt tcacagatt 
      cgccacgat ggcacttag aaaccaaga caaaatgac 
      caaattcgt ctgcacaac caccaataa caaccacga 
      gcaaaaaaa tttagaaaa caagtcact cctccatac 
      ctatctcac taaagggat caaagagaa agaaaccca 
      gccagaatc agaaataac cgagatgga ggtccgcgt 
      ggaccccat gatcatcct ccagtgctc cctcgagac 
      cgaagacga cgaggaaga caaccagag cgaggaagt 
      ctcagacgg cgaaagcca cgtccagag agagaacct 
      ctcagagag cgaaaactg tgacaaata tcgtaaacc 
      atatctttg cccctcgtg tccgaggag tttcacgtc 
      ggagctagc cctccagaa ccacacctg gaaccactc 
      cccgcccat caaacgtat cctcaacag ccacgacaa 
      ctggctgga aaccgaaga aaaagagac tgaagaaaa 
      cgatgcttc cacaaacaa cgacgacga ggacaacat 
      ggaggagtt cgccatgtc gcgcttctc gtgccccat 
      ctgcggcaa ggagatttc gaccaaagg caacctcaa 
      ggttcacct cgagacgca cagacccaa ggggaaata 
      cggctgcga tatttgcgg acgaatatt caagaccca 
      gtgcaactt gtaccgaca caaggagta ccacggcgg 
      gattcagta cccctgcgg ggtatgcgg ccgggtgta 
      ccccacaaa tagcacttt aagggccca ttctatcac 
      tcacagcga cctcaggcc ccacaaatg ccccttgtg 
      cgacaagac gttcaaaag gaaccagga tttgaagtt 
      tcatataaa ccaacatac tggtgctag gccgtatca 
      atgtcccta ctgtcccaa ggctttcgc tagttctgg 
      caactgctt ctcgcaccg gaaacggat gcatccgat 
      tgaggttga gcgggatag ggaacgggc agcgaaaat 
      tatgcgata atttcggtt gattttctt tagaaccaa 
      catgtccgc ctgatgagg tcaacaagt gaggttatg 
      ttctatgtt ctggtgtaa ttatatatg tgtgtatag 
      ataaccggg tgtcatatt caaaattat agagctcta 
      ctttcgtgt cgactcatt tcgagttta gttttggtt 
      gcaaaagtg tcccactct atatggacg ctccctggt 
      gacctgacc tgaccgggg gtgaccgta tggaagtgg 
      gacgactct aaagccttt tacaatttt gtgtaggtc 
      taggtataa gtatcacgg aaaatagta ttattttgg 
      atataaccc cacttggta cgtttttac gtttcgtat 
      tatagtaga tcaactgtg atgaatttt gtatacgat 
      ttttgattt ttactgaaa attatatct agacaaaat 
      atggatgtc tcaaggtgt atggtagaa aattaatgt 
      cattattta gagcaataa ttatttctt ttgggagtc 
      acagtactg acaaaataa agtgacaag gaacaattt 
      tagttatag taaccaatg gctgatact acagtatta 
      tattaatat tagataaac gtgtaaata aagttgttg 
      aataaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY