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Gene information
General information
TC013979 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3417386..3466788
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013979
    iBeetle screen iB_09983

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013979 RA
      MPSPPSDDP ISVECASSS PLSSSCNRT ATFFFSCTD 
      SSTTFVDFY CEFDANTTL CTACDLNST LDLVTNFTA 
      DFSVSDFIL RVVSNYSEC SLVNRSGGV FWTCENVAG 
      DKGAEWGAN YDWSFLFVV VFILAGGLG NILVCLAVI 
      LDRRLQNVT NYFLLSLAI ADLLVSLFV MPLGAIPGF 
      LGKWPFGVA WCTVYVTCD VLACSASIM HMCFISLGR 
      YLGIRNPLK TRHSTTTRT VVMRICLAW LLSMLVSSS 
      ITVLGIIDA RNIMPAPDS CVINNRAFW VFGSLVTFY 
      VPMVIMVVS YALTVQLLR KKARFAADH PENEQFRRL 
      GGRFGPKDR TPAMWRTAG SGERSGNNI RISSSHQQL 
      AYANGGSGT GAAPGARKD QSTQTPENI ARETRNCKL 
      RSLKLQLNN VPSNLSNFR LLAGRVKRK TMAANSVAT 
      EQKASKVLG LVFFTFVLC WAPFFILNI LFAACPDCD 
      VPEHVVVVC LWLGYVSST INPIIYTVF NKTFRAAFI 
      RLLLCRCHR LSRPVRYRS VNENRGAAS LCTPSALPL 
      AISLQGTPL LTPASTESS YVRTPSTNF RERDDSYDD 
      HDC
    CDS sequence
    • >TC013979 RA
      atgccgtcg cctccgtcg gacgacccc atctccgtc 
      gaatgcgcc tccagctcg cccctgtca tcctcatgc 
      aaccgcacc gccaccttc ttcttctcc tgcaccgac 
      tccagtacg accttcgtc gacttctac tgcgagttc 
      gacgccaac accaccctc tgcaccgcc tgcgacctg 
      aactcgacc ctggaccta gtgacaaac ttcacggct 
      gatttcagt gtttccgat tttattttg cgtgtcgtg 
      tccaattac tccgagtgc agtttggtt aatcgcagt 
      gggggtgtg ttctggacg tgcgaaaat gtcgcagga 
      gataagggg gcggaatgg ggcgcgaat tacgactgg 
      agttttctg ttcgtagtg gtgtttatt ttggccgga 
      ggactggga aatatcctg gtgtgtctg gcggttatt 
      ttggacaga aggctgcag aatgtgacc aattacttc 
      ctgctgtcg ctggccatc gccgacctt ttggtcagt 
      ctcttcgtc atgccgttg ggggcgatc ccaggattc 
      ctcggtaag tggcccttt ggcgtcgcc tggtgtacc 
      gtatatgtc acgtgcgac gtcctcgcc tgttccgcg 
      tccatcatg cacatgtgc ttcatcagc ctgggtcgt 
      tatctcggc ataagaaac cctctcaag acgcgccac 
      agcaccacc actcggacg gtggtaatg aggatatgt 
      ctcgcctgg ctgctgtcg atgctagtc tcgagctcg 
      atcacagtc ctaggcata atcgacgcc cgcaacata 
      atgcccgcc cccgactcg tgcgtgatc aacaaccgg 
      gcgttctgg gtgttcggc tcgctggtc actttctac 
      gtcccgatg gtgatcatg gtcgtatcg tacgccctc 
      accgtccaa ctcctccgc aagaaggcg cgcttcgcc 
      gccgaccac ccggaaaac gagcaattc cggcgactc 
      gggggccgc ttcgggccg aaggaccgc actccggcc 
      atgtggagg actgccggt tccggggag aggtccggg 
      aataacatc aggattagt tcgtcgcat caacagctc 
      gcctatgcc aacggcggg agtggcact ggggccgct 
      ccgggcgcc cggaaagac caaagcaca caaacgccg 
      gaaaatatc gccagagag acgaggaat tgcaagttg 
      aggtcgctg aagctgcag ctcaataat gtgccgtcg 
      aatttgtcc aatttcagg ctcttggcc ggtcgtgtc 
      aagcgcaaa actatggca gctaatagt gtcgccacc 
      gagcagaag gcctccaaa gtactcggt ttggtcttc 
      ttcacgttc gtcctctgt tgggcacct tttttcatc 
      ctcaacatc ctttttgcc gcttgtcct gactgcgac 
      gtcccggaa cacgtcgtc gtcgtgtgt ctctggctg 
      ggatacgtc agctccacc atcaatccc atcatttat 
      actgtgttt aataagacg tttcgagcg gctttcatc 
      cgcctttta ctatgcaga tgtcacaga ctttccaga 
      ccggtgagg tacagatcg gtcaacgaa aaccggggg 
      gctgccagt ctgtgcact ccttcggcc cttccattg 
      gctataagt cttcaaggg actcctttg ctaactcca 
      gcttcgacg gaatcttcc tacgtcagg accccgtcg 
      acgaatttc cgagaaagg gacgattca tacgacgat 
      catgactgt tga
    mRNA sequence
    • >TC013979 RA
      tgctaggtt tggacgcgg tttcgctcc ggaggaaaa 
      acacgcgtc gtgaacgga aaatcaaaa atgtgacac 
      ttttcctat gaaaaataa ttcgtgacc gagcaactt 
      taccaatta ccaagtgct gaaagtccg tgcttgatg 
      gctccgcac tagaacaat gccgtcgcc tccgtcgga 
      cgaccccat ctccgtcga atgcgcctc cagctcgcc 
      cctgtcatc ctcatgcaa ccgcaccgc caccttctt 
      cttctcctg caccgactc cagtacgac cttcgtcga 
      cttctactg cgagttcga cgccaacac caccctctg 
      caccgcctg cgacctgaa ctcgaccct ggacctagt 
      gacaaactt cacggctga tttcagtgt ttccgattt 
      tattttgcg tgtcgtgtc caattactc cgagtgcag 
      tttggttaa tcgcagtgg gggtgtgtt ctggacgtg 
      cgaaaatgt cgcaggaga taagggggc ggaatgggg 
      cgcgaatta cgactggag ttttctgtt cgtagtggt 
      gtttatttt ggccggagg actgggaaa tatcctggt 
      gtgtctggc ggttatttt ggacagaag gctgcagaa 
      tgtgaccaa ttacttcct gctgtcgct ggccatcgc 
      cgacctttt ggtcagtct cttcgtcat gccgttggg 
      ggcgatccc aggattcct cggtaagtg gccctttgg 
      cgtcgcctg gtgtaccgt atatgtcac gtgcgacgt 
      cctcgcctg ttccgcgtc catcatgca catgtgctt 
      catcagcct gggtcgtta tctcggcat aagaaaccc 
      tctcaagac gcgccacag caccaccac tcggacggt 
      ggtaatgag gatatgtct cgcctggct gctgtcgat 
      gctagtctc gagctcgat cacagtcct aggcataat 
      cgacgcccg caacataat gcccgcccc cgactcgtg 
      cgtgatcaa caaccgggc gttctgggt gttcggctc 
      gctggtcac tttctacgt cccgatggt gatcatggt 
      cgtatcgta cgccctcac cgtccaact cctccgcaa 
      gaaggcgcg cttcgccgc cgaccaccc ggaaaacga 
      gcaattccg gcgactcgg gggccgctt cgggccgaa 
      ggaccgcac tccggccat gtggaggac tgccggttc 
      cggggagag gtccgggaa taacatcag gattagttc 
      gtcgcatca acagctcgc ctatgccaa cggcgggag 
      tggcactgg ggccgctcc gggcgcccg gaaagacca 
      aagcacaca aacgccgga aaatatcgc cagagagac 
      gaggaattg caagttgag gtcgctgaa gctgcagct 
      caataatgt gccgtcgaa tttgtccaa tttcaggct 
      cttggccgg tcgtgtcaa gcgcaaaac tatggcagc 
      taatagtgt cgccaccga gcagaaggc ctccaaagt 
      actcggttt ggtcttctt cacgttcgt cctctgttg 
      ggcaccttt tttcatcct caacatcct ttttgccgc 
      ttgtcctga ctgcgacgt cccggaaca cgtcgtcgt 
      cgtgtgtct ctggctggg atacgtcag ctccaccat 
      caatcccat catttatac tgtgtttaa taagacgtt 
      tcgagcggc tttcatccg ccttttact atgcagatg 
      tcacagact ttccagacc ggtgaggta cagatcggt 
      caacgaaaa ccggggggc tgccagtct gtgcactcc 
      ttcggccct tccattggc tataagtct tcaagggac 
      tcctttgct aactccagc ttcgacgga atcttccta 
      cgtcaggac cccgtcgac gaatttccg agaaaggga 
      cgattcata cgacgatca tgactgttg aacttgtgt 
      ttcactgtt acctgaatc tctcttcaa gtagtcgaa 
      tttaatgta atttctcaa cggtcaaga gatgactta 
      attaaccaa agatgtgtt tcgatgttg ttccaatcc 
      agtagaaag aaaggtacc aaatgacaa tctttgata 
      cttgcgaca cattcctct acgaacgtt ttttgcgag 
      gatctgtct ctttcaaag acaaatctt gtgatgtta 
      gttcgtaat aacaaatcg gccagttgc aatcagcaa 
      taaataggt tataaaccg aaactaatt tttgtaaaa 
      caaacattt ctcgatgtg aaaaataaa tgtgtgaaa 
      aaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY