Status
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Gene information
General information
TC031481 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1305839..1312734
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031481
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09980

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva completeeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031481 RA
      MPTLPPMHP DNGLIFTPP DVLKQRIMV AEFVGRGQS 
      GSPVNGEIP QLNSNMPAS HGRELLSSS TSPDADSFP 
      PGYNMNSVK HKELFSQRK QREFTPDSK KDDSYWDRR 
      RRNNEAAKR SREKRRFND MVLEQRVVE LTKENAILK 
      AQLEAIKEE YGICGENVV CVEKVLANL PTNEQVLSL 
      TKRQKLTHS ANPLLFNPH SPIPTPVIH QPVLGXXXX 
      XXXXXXXXX XXXXTHTFG NPLLFNPHS PIPTPVIHQ 
      PVLGSPPPQ PLPHAHILH PPASHHEPP LQRPPRGLP 
      LSLPQLPPH SPLRLQRCP QSLRAVAVT RPESFRSL
    • >TC031481 RB
      MVAEFVGRG QSGSPVNGE IPQLNSNMP ASHGRELLS 
      SSTSPDADS FPPGYNMNS VKHKELFSQ RKQREFTPD 
      SKKDDSYWD RRRRNNEAA KRSREKRRF NDMVLEQRV 
      VELTKENAI LKAQLEAIK EEYGICGEN VVCVEKVLA 
      NLPTNEQVL SLTKRQKLT HSANPLLFN PHSPIPTPV 
      IHQPVLGXX XXXXXXXXX XXXXXXTHT FGNPLLFNP 
      HSPIPTPVI HQPVLGSPP PQPLPHAHI LHPPASHHE 
      PPLQRPPRG LPLSLPQLP PHSPLRLQR CPQSLRAVA 
      VTRPESFRS L
    CDS sequence
    • >TC031481 RA
      atgcccacc ctgccgccg atgcacccc gataacgga 
      ttaattttc acaccccca gatgtatta aagcagcgt 
      ataatggtt gccgaattc gtgggccga ggtcaaagt 
      ggatctcca gtaaatggt gaaatccca caactaaac 
      tcaaacatg cctgccagc cacggacgg gagctcctc 
      tcatcgtcg acttctccc gacgccgac tctttccct 
      cccggctac aacatgaac tccgtcaaa cacaaagag 
      ctcttctcc cagcgaaag cagcgcgag ttcaccccc 
      gacagcaag aaggacgac agctattgg gaccgccgg 
      cgccgaaat aacgaagcc gccaaacgc tcccgcgaa 
      aagcgccgc ttcaacgac atggtcctc gaacagcgc 
      gtcgtcgaa ctcactaaa gagaacgcc atcttgaag 
      gcccagtta gaggccatc aaggaagag tacgggatc 
      tgtggcgaa aacgtcgtt tgcgtcgag aaagtcctc 
      gcaaatctc cccacaaac gagcaagta cttagcctc 
      accaaacgt cagaaactc acacattcg gcaaatccg 
      ctcctcttc aacccccac agtccgatc ccgaccccg 
      gtgatccac cagccggtt ttggggnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnaactcac acattcggc aatccgctc ctcttcaac 
      ccccacagt ccgatcccg accccggtg atccaccag 
      ccggttttg gggtcgcct ccgccgcag cccctcccg 
      cacgcccac atcctccac ccgcccgcg tcccaccac 
      gagccgccc ttacagaga ccaccccga ggcctaccc 
      ctatcccta ccccaacta ccaccccac agtccacta 
      cgactccaa cgatgccct caatctctc cgcgcggtc 
      gcggtcacg aggccagag tcctttcga agtctctag
    • >TC031481 RB
      atggttgcc gaattcgtg ggccgaggt caaagtgga 
      tctccagta aatggtgaa atcccacaa ctaaactca 
      aacatgcct gccagccac ggacgggag ctcctctca 
      tcgtcgact tctcccgac gccgactct ttccctccc 
      ggctacaac atgaactcc gtcaaacac aaagagctc 
      ttctcccag cgaaagcag cgcgagttc acccccgac 
      agcaagaag gacgacagc tattgggac cgccggcgc 
      cgaaataac gaagccgcc aaacgctcc cgcgaaaag 
      cgccgcttc aacgacatg gtcctcgaa cagcgcgtc 
      gtcgaactc actaaagag aacgccatc ttgaaggcc 
      cagttagag gccatcaag gaagagtac gggatctgt 
      ggcgaaaac gtcgtttgc gtcgagaaa gtcctcgca 
      aatctcccc acaaacgag caagtactt agcctcacc 
      aaacgtcag aaactcaca cattcggca aatccgctc 
      ctcttcaac ccccacagt ccgatcccg accccggtg 
      atccaccag ccggttttg gggnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnna 
      actcacaca ttcggcaat ccgctcctc ttcaacccc 
      cacagtccg atcccgacc ccggtgatc caccagccg 
      gttttgggg tcgcctccg ccgcagccc ctcccgcac 
      gcccacatc ctccacccg cccgcgtcc caccacgag 
      ccgccctta cagagacca ccccgaggc ctaccccta 
      tccctaccc caactacca ccccacagt ccactacga 
      ctccaacga tgccctcaa tctctccgc gcggtcgcg 
      gtcacgagg ccagagtcc tttcgaagt ctctag
    mRNA sequence
    • >TC031481 RA
      tgtgtgctt ccaactcta tgtgtggtg ttgtgctgg 
      ttctggttc tgattccga ttagaaggc ctcatcatt 
      gcgtcactc cacagcgcc aggccggcc ggccctggt 
      cccttcatg atgcccacc ctgccgccg atgcacccc 
      gataacgga ttaattttc acaccccca gatgtatta 
      aagcagcgt ataatggtt gccgaattc gtgggccga 
      ggtcaaagt ggatctcca gtaaatggt gaaatccca 
      caactaaac tcaaacatg cctgccagc cacggacgg 
      gagctcctc tcatcgtcg acttctccc gacgccgac 
      tctttccct cccggctac aacatgaac tccgtcaaa 
      cacaaagag ctcttctcc cagcgaaag cagcgcgag 
      ttcaccccc gacagcaag aaggacgac agctattgg 
      gaccgccgg cgccgaaat aacgaagcc gccaaacgc 
      tcccgcgaa aagcgccgc ttcaacgac atggtcctc 
      gaacagcgc gtcgtcgaa ctcactaaa gagaacgcc 
      atcttgaag gcccagtta gaggccatc aaggaagag 
      tacgggatc tgtggcgaa aacgtcgtt tgcgtcgag 
      aaagtcctc gcaaatctc cccacaaac gagcaagta 
      cttagcctc accaaacgt cagaaactc acacattcg 
      gcaaatccg ctcctcttc aacccccac agtccgatc 
      ccgaccccg gtgatccac cagccggtt ttggggnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnaactcac acattcggc aatccgctc 
      ctcttcaac ccccacagt ccgatcccg accccggtg 
      atccaccag ccggttttg gggtcgcct ccgccgcag 
      cccctcccg cacgcccac atcctccac ccgcccgcg 
      tcccaccac gagccgccc ttacagaga ccaccccga 
      ggcctaccc ctatcccta ccccaacta ccaccccac 
      agtccacta cgactccaa cgatgccct caatctctc 
      cgcgcggtc gcggtcacg aggccagag tcctttcga 
      agtctctag taattcggg ggatgagag cgcccccgt 
      ggctcttac ctctgaagc caacaacag cctcccgtt 
      gaaattgcg ccacaagtc ccacctggg ggacaagga 
      cgccgccaa taccctcct gtcgctcca gaatattaa 
      acaggagcc gggaccgcg cgctagtcc tccttggga 
      cgccgaagg ctcgagcga cgagcgtga ctctgggat 
      ctctttggg ggcggaatg gtcgacgca ggccgcgca 
      agccgccgc cactttgca aactcttaa acagtccca 
      cctccaggc catgcagga ggccgctgg tggcgacgc 
      cgagaccaa gcatagaat ccactccga aattgtgag 
      gttatcgtc cgaggttga acatctcaa gtcgatgat 
      gatcggcaa ggagaagga accgagaca ttagtttga 
      tggggtcgg accctgaaa gggtgcccc agtgctcgt 
      cgtgcgcgt acttgtgat ggatcggac atatcaacg 
      ctccgccgg gagctattt attctagtg ttaagtatt 
      tattcattt ttgtaagtt tggagatct ctccgcttg 
      cacaactct gtatcttag tggacttgc tttcatttt 
      ttgccaata tattcctac gagtttatt taacataat 
      cttattgta acgcattgt tggcgcttt atcgctctg 
      tactatatt ctaccaatg gttttcatt ttcctcatc 
      tcataggga tttattgta cctttttat tttttaact 
      ggaaatgtg tgatttttt tgtagacta ttactatta 
      agaaaatac gattattta tgtataaat atgtatgta 
      tttttgtta catcatcgg ccaaaaccg aaatttacg 
      ttatgttat ctcattatt gtatattta tcaataaga 
      cttcagccg gaccttacc tcaactgat gcaaaaaac 
      tgatctgta ataaccgat ttcgtccgg cacactaca 
      atatcacct taataaaga aaagtaacg atatatttt 
      catattacc aagcatgta tcctcttaa ctatgtatt 
      aaataaata tttgcatcg ttt
    • >TC031481 RB
      gcggccact gtcaaagtg tagtcagtt tcggaacgc 
      tgactgttc agttctcgc cagttccac tcagagtac 
      agacctagt tgagtgtcg ctactgttt gtgtattgt 
      tattgtttg atagtgatt ttttaacaa cgaaactaa 
      acaaatccc gggacttta cacgtcaac aattgatag 
      ttgatgtat taaagcagc gtataatgg ttgccgaat 
      tcgtgggcc gaggtcaaa gtggatctc cagtaaatg 
      gtgaaatcc cacaactaa actcaaaca tgcctgcca 
      gccacggac gggagctcc tctcatcgt cgacttctc 
      ccgacgccg actctttcc ctcccggct acaacatga 
      actccgtca aacacaaag agctcttct cccagcgaa 
      agcagcgcg agttcaccc ccgacagca agaaggacg 
      acagctatt gggaccgcc ggcgccgaa ataacgaag 
      ccgccaaac gctcccgcg aaaagcgcc gcttcaacg 
      acatggtcc tcgaacagc gcgtcgtcg aactcacta 
      aagagaacg ccatcttga aggcccagt tagaggcca 
      tcaaggaag agtacggga tctgtggcg aaaacgtcg 
      tttgcgtcg agaaagtcc tcgcaaatc tccccacaa 
      acgagcaag tacttagcc tcaccaaac gtcagaaac 
      tcacacatt cggcaaatc cgctcctct tcaaccccc 
      acagtccga tcccgaccc cggtgatcc accagccgg 
      ttttggggn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnaactc acacattcg 
      gcaatccgc tcctcttca acccccaca gtccgatcc 
      cgaccccgg tgatccacc agccggttt tggggtcgc 
      ctccgccgc agcccctcc cgcacgccc acatcctcc 
      acccgcccg cgtcccacc acgagccgc ccttacaga 
      gaccacccc gaggcctac ccctatccc taccccaac 
      taccacccc acagtccac tacgactcc aacgatgcc 
      ctcaatctc tccgcgcgg tcgcggtca cgaggccag 
      agtcctttc gaagtctct agtaattcg ggggatgag 
      agcgccccc gtggctctt acctctgaa gccaacaac 
      agcctcccg ttgaaattg cgccacaag tcccacctg 
      ggggacaag gacgccgcc aataccctc ctgtcgctc 
      cagaatatt aaacaggag ccgggaccg cgcgctagt 
      cctccttgg gacgccgaa ggctcgagc gacgagcgt 
      gactctggg atctctttg ggggcggaa tggtcgacg 
      caggccgcg caagccgcc gccactttg caaactctt 
      aaacagtcc cacctccag gccatgcag gaggccgct 
      ggtggcgac gccgagacc aagcataga atccactcc 
      gaaattgtg aggttatcg tccgaggtt gaacatctc 
      aagtcgatg atgatcggc aaggagaag gaaccgaga 
      cattagttt gatggggtc ggaccctga aagggtgcc 
      ccagtgctc gtcgtgcgc gtacttgtg atggatcgg 
      acatatcaa cgctccgcc gggagctat ttattctag 
      tgttaagta tttattcat ttttgtaag tttggagat 
      ctctccgct tgcacaact ctgtatctt agtggactt 
      gctttcatt ttttgccaa tatattcct acgagttta 
      tttaacata atcttattg taacgcatt gttggcgct 
      ttatcgctc tgtactata ttctaccaa tggttttca 
      ttttcctca tctcatagg gatttattg taccttttt 
      attttttaa ctggaaatg tgtgatttt tttgtagac 
      tattactat taagaaaat acgattatt tatgtataa 
      atatgtatg tatttttgt tacatcatc ggccaaaac 
      cgaaattta cgttatgtt atctcatta ttgtatatt 
      tatcaataa gacttcagc cggacctta cctcaactg 
      atgcaaaaa actgatctg taataaccg atttcgtcc 
      ggcacacta caatatcac cttaataaa gaaaagtaa 
      cgatatatt ttcatatta ccaagcatg tatcctctt 
      aactatgta ttaaataaa tatttgcat cgttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY