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Gene information
General information
TC013947 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2760660..2761938
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013947
    iBeetle screen iB_09978

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013947 RA
      MGFPPVPPV IKSIAHVLK VADEHESRD IVVSYWARM 
      YACQSAMKL IPGKKPPEV SNLLIALMD WLETTKKSH 
      HDLEGITNE TVAQAMIEN YAMQLFTFA DAQDRAENF 
      NKNMIKAFY TAGILMDIL EQFGEQSEE IINKKKYAK 
      WKAAYIHNC LKSGDKPTS GGPDDHLKN VIDVSKGED 
      DASQNTLLI SPRPYRDFP THVGYNPPT GPAPASSPV 
      TPVVPPEPQ EFTPQPEPP TPPVATTNN DGGFAPGPE 
      QIQKAQKYC KYATSALNY DDVKTAIEN LTKALSLLQ 
      AGKE
    CDS sequence
    • >TC013947 RA
      atgggtttt ccgccagtt ccgcccgta attaagtcc 
      attgcccac gttttgaaa gtggcagat gagcatgaa 
      agccgtgac attgttgtg agctattgg gctcgcatg 
      tatgcttgc cagtcggct atgaagcta attcctggc 
      aaaaaacca ccagaagtc tcgaatcta ttaattgct 
      ttgatggac tggttagag acaacgaag aagtcccac 
      catgatctt gaagggatt actaatgag accgtagcc 
      caggctatg atcgagaat tacgccatg cagttattt 
      acgtttgct gacgctcaa gaccgggct gaaaatttc 
      aacaaaaac atgattaag gcgttctac accgctggc 
      atcctaatg gacattctg gagcagttc ggcgagcag 
      tccgaggaa ataatcaat aagaaaaaa tacgcgaaa 
      tggaaggct gcatacatc cataactgt ctcaaatcc 
      ggggataag cccacttct gggggcccc gacgaccac 
      ttgaagaac gtcattgac gtgtctaag ggtgaagac 
      gacgcttcc caaaacact ctccttatt tcgcccaga 
      ccttaccgc gacttcccc actcatgtg ggctacaac 
      ccccccaca ggaccagcc cctgcctcc tcgcccgtc 
      actccagtc gtgccacct gagccccaa gagttcacc 
      ccgcagcct gagcccccc acaccgcca gtcgccacc 
      accaacaac gacgggggt tttgcacca ggtccagaa 
      cagatacaa aaggcccag aagtattgt aaatacgca 
      acttcggcg cttaattac gacgacgtt aaaaccgca 
      attgagaac cttacaaag gcgttaagc ctgttgcag 
      gctggtaag gaatag
    mRNA sequence
    • >TC013947 RA
      cgagtaaac caaaaccaa aacattgaa tttgtgata 
      gtttaagcc tttgaggta aatttggga gtgaaaaat 
      gggttttcc gccagttcc gcccgtaat taagtccat 
      tgcccacgt tttgaaagt ggcagatga gcatgaaag 
      ccgtgacat tgttgtgag ctattgggc tcgcatgta 
      tgcttgcca gtcggctat gaagctaat tcctggcaa 
      aaaaccacc agaagtctc gaatctatt aattgcttt 
      gatggactg gttagagac aacgaagaa gtcccacca 
      tgatcttga agggattac taatgagac cgtagccca 
      ggctatgat cgagaatta cgccatgca gttatttac 
      gtttgctga cgctcaaga ccgggctga aaatttcaa 
      caaaaacat gattaaggc gttctacac cgctggcat 
      cctaatgga cattctgga gcagttcgg cgagcagtc 
      cgaggaaat aatcaataa gaaaaaata cgcgaaatg 
      gaaggctgc atacatcca taactgtct caaatccgg 
      ggataagcc cacttctgg gggccccga cgaccactt 
      gaagaacgt cattgacgt gtctaaggg tgaagacga 
      cgcttccca aaacactct ccttatttc gcccagacc 
      ttaccgcga cttccccac tcatgtggg ctacaaccc 
      ccccacagg accagcccc tgcctcctc gcccgtcac 
      tccagtcgt gccacctga gccccaaga gttcacccc 
      gcagcctga gccccccac accgccagt cgccaccac 
      caacaacga cgggggttt tgcaccagg tccagaaca 
      gatacaaaa ggcccagaa gtattgtaa atacgcaac 
      ttcggcgct taattacga cgacgttaa aaccgcaat 
      tgagaacct tacaaaggc gttaagcct gttgcaggc 
      tggtaagga atagactcc gtttttatt tcgcttgta 
      tttgtgtaa ttaacacta ataaatgta agaatattt 
      atctttttt gcactagtt ggaaaaaca gtagattcg 
      gctgtgaca aaataatca acttttgtc gagtttata 
      atagttgtt gaatagttt agattgttt ttgtcctaa 
      gccgcgatg gccttactg taagcattt ttcaagctt 
      tatttttga ataatacac gtttgaatt aatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY