General information | |||
TC013871 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 2628452..2633257 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC013871 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_09975 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
mature egg not present - Number of animals showing the phenotype: 2
egg chamber mostlynot present - Number of animals showing the phenotype: 2
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC013871 RA
MEVKIEPSV FDINKICRA CLSEKGEMR SVFLTDEST GHAMILAEM ITGISSVQI DNGDGLPSL VCLQCVHQL ARAYSFKQL CEQSDTNLR QYLGLPPSM KQKKEEETT HPTDFATTL LLDNFGLDS SSGDSDDDD YKIDYNLLQ NSLSENAND EKAIAQRQL LKAAKAQKS KLSKKLLAK AGGKAAVLA AKKLKRNTN QCNVCKKQF INMKSFRKH LRTHIEDRP YKCKLCPRG FTEENYLNN HMRTHVPED QKPHECKVC KKRFIHTTL LTKHMLKHS GEKPFVCKI CNKGCYAEN SLLKHMKIH EKKEGDPAL LKHICDYCK TEFPDAQSL SVHIKQHTG DRPFVCNIC GKCFPQRFN LELHLRTHT GERPFQCEV CKNGYVSKA SLKIHMRTH TNERPFVCD FCGKAFRQS GDLTSHKRL HGTEKPIEC QVCQKRFTT VMKLKYHMR NHTGERPYV CTVCGRGFT VNTILLRHM RVHSGERPY VCVTCGKAF SQSSTLNTH MKVHAPSPK FTNQESQQR IPKFHTTNE QKFQNQQHP PPQQAPDHS NRLMQTDNR MMQSEPQRM MPQDNTRMI SNDGSRIIT DNSHLIADN KGLINDGTR LLVNMTEPP RLLVHDNSR FVTNDNRLL TNDGRLLTN VNDNSRLLT NDNARHLAV IESSRILTD KYLASYEPY HRGHL
- >TC013871 RA
atggaggtt aaaatagaa ccaagcgta ttcgatata aataagata tgtagggcc tgtctctcg gaaaaaggc gaaatgagg tccgtgttc ctgaccgat gaatctacc ggacatgct atgatactt gcagaaatg atcacggga atttcctcc gtacagatt gacaacggc gacgggttg ccctccttg gtgtgcctc caatgcgtc caccagctg gctagagcc tactccttc aagcagctt tgcgagcag tccgacacc aatctgcgc caataccta ggcctgccg ccaagtatg aagcagaag aaagaagag gagaccaca catcccacc gactttgcc acaaccctc cttcttgat aatttcggc ttggactcg tcatccggt gatagtgac gatgatgat tacaaaatc gactacaat ttactccag aattcgtta tccgagaac gccaacgac gaaaaagcc atcgcccag agacaactc ctcaaggct gccaaggcg cagaagagc aaactgtcg aagaagttg ctcgcaaag gccggggga aaagcagct gttctggcg gcaaaaaaa ctgaagcgc aacaccaac caatgcaac gtgtgcaag aaacagttt attaatatg aagtcgttc cgcaagcac ttgcggacg cacatcgag gaccgtccg tacaaatgc aagctgtgt ccccgggga ttcaccgag gaaaactac ctgaacaac cacatgcgc acccacgtg cctgaagac cagaagccg catgagtgc aaagtgtgc aaaaaacgg ttcattcac actacactc ctcaccaaa cacatgctg aagcacagc ggggagaag ccgttcgtg tgcaagatt tgcaacaag gggtgttat gccgaaaat agtcttctc aagcacatg aaaatccac gaaaagaaa gagggggat ccggcgttg cttaaacac atttgtgac tactgtaaa actgaattt cccgatgct cagagtttg tcggttcat ataaaacag catacgggg gatcggccc tttgtgtgc aacatttgc ggcaagtgc ttccctcag aggttcaat cttgagttg catctaagg acgcatacg ggagaacgg ccgttccag tgtgaggtt tgcaaaaac ggttacgtt tccaaagct agtttgaag attcacatg cggacgcat acgaatgag cggccgttt gtgtgcgat ttttgcggg aaagctttc agacaatcg ggcgatttg acgagtcat aagcgcctc catgggacg gagaaaccg atagaatgc caagtctgc cagaaaaga tttacgacc gtaatgaag ctcaagtac cacatgagg aaccacacg ggggagcgg ccctacgtt tgtaccgtg tgtggtcgg ggcttcacc gtgaacacg attttattg cggcacatg agagtgcac tcaggggag cgtccatac gtttgcgtg acgtgtggt aaagctttt tcgcaatcg agcactctt aacacgcac atgaaggtg catgcacca tctccgaaa ttcaccaac caagagtcg cagcaaaga attccgaaa ttccacaca accaacgaa caaaagttc caaaaccaa caacacccg cctccacag caagccccc gaccatagc aatcgttta atgcaaacg gataatcgc atgatgcag tcggaaccg caacgtatg atgcctcaa gacaacact cggatgatc tcgaatgat ggctccagg attataacg gataattca caccttatc gccgataat aaaggtttg attaatgat gggaccaga ttgttggtt aatatgacg gagccgccg cggcttcta gttcacgat aattcgagg tttgtaacg aatgataat cggttgttg acgaacgac ggacgcttg ctgactaat gttaacgat aattcgagg cttttgacg aatgacaat gcgaggcat ttagccgta atcgaaagt tcgaggatt ttaaccgat aagtattta gctagttac gagccatat catcgaggg catttgtaa
- >TC013871 RA
acaatcgac cagtggaag aatctattt tcgccattt cctttgcgt gtttgtgca tcttttgtt gtgtttata tttgtgatg taatttaac ttttgcgat ggaggttaa aatagaacc aagcgtatt cgatataaa taagatatg tagggcctg tctctcgga aaaaggcga aatgaggtc cgtgttcct gaccgatga atctaccgg acatgctat gatacttgc agaaatgat cacgggaat ttcctccgt acagattga caacggcga cgggttgcc ctccttggt gtgcctcca atgcgtcca ccagctggc tagagccta ctccttcaa gcagctttg cgagcagtc cgacaccaa tctgcgcca atacctagg cctgccgcc aagtatgaa gcagaagaa agaagagga gaccacaca tcccaccga ctttgccac aaccctcct tcttgataa tttcggctt ggactcgtc atccggtga tagtgacga tgatgatta caaaatcga ctacaattt actccagaa ttcgttatc cgagaacgc caacgacga aaaagccat cgcccagag acaactcct caaggctgc caaggcgca gaagagcaa actgtcgaa gaagttgct cgcaaaggc cgggggaaa agcagctgt tctggcggc aaaaaaact gaagcgcaa caccaacca atgcaacgt gtgcaagaa acagtttat taatatgaa gtcgttccg caagcactt gcggacgca catcgagga ccgtccgta caaatgcaa gctgtgtcc ccggggatt caccgagga aaactacct gaacaacca catgcgcac ccacgtgcc tgaagacca gaagccgca tgagtgcaa agtgtgcaa aaaacggtt cattcacac tacactcct caccaaaca catgctgaa gcacagcgg ggagaagcc gttcgtgtg caagatttg caacaaggg gtgttatgc cgaaaatag tcttctcaa gcacatgaa aatccacga aaagaaaga gggggatcc ggcgttgct taaacacat ttgtgacta ctgtaaaac tgaatttcc cgatgctca gagtttgtc ggttcatat aaaacagca tacggggga tcggccctt tgtgtgcaa catttgcgg caagtgctt ccctcagag gttcaatct tgagttgca tctaaggac gcatacggg agaacggcc gttccagtg tgaggtttg caaaaacgg ttacgtttc caaagctag tttgaagat tcacatgcg gacgcatac gaatgagcg gccgtttgt gtgcgattt ttgcgggaa agctttcag acaatcggg cgatttgac gagtcataa gcgcctcca tgggacgga gaaaccgat agaatgcca agtctgcca gaaaagatt tacgaccgt aatgaagct caagtacca catgaggaa ccacacggg ggagcggcc ctacgtttg taccgtgtg tggtcgggg cttcaccgt gaacacgat tttattgcg gcacatgag agtgcactc aggggagcg tccatacgt ttgcgtgac gtgtggtaa agctttttc gcaatcgag cactcttaa cacgcacat gaaggtgca tgcaccatc tccgaaatt caccaacca agagtcgca gcaaagaat tccgaaatt ccacacaac caacgaaca aaagttcca aaaccaaca acacccgcc tccacagca agcccccga ccatagcaa tcgtttaat gcaaacgga taatcgcat gatgcagtc ggaaccgca acgtatgat gcctcaaga caacactcg gatgatctc gaatgatgg ctccaggat tataacgga taattcaca ccttatcgc cgataataa aggtttgat taatgatgg gaccagatt gttggttaa tatgacgga gccgccgcg gcttctagt tcacgataa ttcgaggtt tgtaacgaa tgataatcg gttgttgac gaacgacgg acgcttgct gactaatgt taacgataa ttcgaggct tttgacgaa tgacaatgc gaggcattt agccgtaat cgaaagttc gaggatttt aaccgataa gtatttagc tagttacga gccatatca tcgagggca tttgtaaag tgtcttcgt gcttgcgtt tgattacac aattattta tagtgtact ttagaggta ataataatt tattggtcg atgtatttt tttaaggga agtatcgta tttaataat tggttgatt aaatggtac aaaaatttg aagaaatta agttcattt tctaatatt ttgaggaaa taatagtta tttttgtaa
