Status
Please wait ...
Gene information
General information
TC013871 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2628452..2633257
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013871
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09975

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    mature egg not present - Number of animals showing the phenotype: 2

    egg chamber mostlynot present - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013871 RA
      MEVKIEPSV FDINKICRA CLSEKGEMR SVFLTDEST 
GHAMILAEM ITGISSVQI DNGDGLPSL VCLQCVHQL 
ARAYSFKQL CEQSDTNLR QYLGLPPSM KQKKEEETT 
HPTDFATTL LLDNFGLDS SSGDSDDDD YKIDYNLLQ 
NSLSENAND EKAIAQRQL LKAAKAQKS KLSKKLLAK 
AGGKAAVLA AKKLKRNTN QCNVCKKQF INMKSFRKH 
LRTHIEDRP YKCKLCPRG FTEENYLNN HMRTHVPED 
QKPHECKVC KKRFIHTTL LTKHMLKHS GEKPFVCKI 
CNKGCYAEN SLLKHMKIH EKKEGDPAL LKHICDYCK 
TEFPDAQSL SVHIKQHTG DRPFVCNIC GKCFPQRFN 
LELHLRTHT GERPFQCEV CKNGYVSKA SLKIHMRTH 
TNERPFVCD FCGKAFRQS GDLTSHKRL HGTEKPIEC 
QVCQKRFTT VMKLKYHMR NHTGERPYV CTVCGRGFT 
VNTILLRHM RVHSGERPY VCVTCGKAF SQSSTLNTH 
MKVHAPSPK FTNQESQQR IPKFHTTNE QKFQNQQHP 
PPQQAPDHS NRLMQTDNR MMQSEPQRM MPQDNTRMI 
SNDGSRIIT DNSHLIADN KGLINDGTR LLVNMTEPP 
RLLVHDNSR FVTNDNRLL TNDGRLLTN VNDNSRLLT 
NDNARHLAV IESSRILTD KYLASYEPY HRGHL
    CDS sequence
    • >TC013871 RA
      atggaggtt aaaatagaa ccaagcgta ttcgatata 
aataagata tgtagggcc tgtctctcg gaaaaaggc 
gaaatgagg tccgtgttc ctgaccgat gaatctacc 
ggacatgct atgatactt gcagaaatg atcacggga 
atttcctcc gtacagatt gacaacggc gacgggttg 
ccctccttg gtgtgcctc caatgcgtc caccagctg 
gctagagcc tactccttc aagcagctt tgcgagcag 
tccgacacc aatctgcgc caataccta ggcctgccg 
ccaagtatg aagcagaag aaagaagag gagaccaca 
catcccacc gactttgcc acaaccctc cttcttgat 
aatttcggc ttggactcg tcatccggt gatagtgac 
gatgatgat tacaaaatc gactacaat ttactccag 
aattcgtta tccgagaac gccaacgac gaaaaagcc 
atcgcccag agacaactc ctcaaggct gccaaggcg 
cagaagagc aaactgtcg aagaagttg ctcgcaaag 
gccggggga aaagcagct gttctggcg gcaaaaaaa 
ctgaagcgc aacaccaac caatgcaac gtgtgcaag 
aaacagttt attaatatg aagtcgttc cgcaagcac 
ttgcggacg cacatcgag gaccgtccg tacaaatgc 
aagctgtgt ccccgggga ttcaccgag gaaaactac 
ctgaacaac cacatgcgc acccacgtg cctgaagac 
cagaagccg catgagtgc aaagtgtgc aaaaaacgg 
ttcattcac actacactc ctcaccaaa cacatgctg 
aagcacagc ggggagaag ccgttcgtg tgcaagatt 
tgcaacaag gggtgttat gccgaaaat agtcttctc 
aagcacatg aaaatccac gaaaagaaa gagggggat 
ccggcgttg cttaaacac atttgtgac tactgtaaa 
actgaattt cccgatgct cagagtttg tcggttcat 
ataaaacag catacgggg gatcggccc tttgtgtgc 
aacatttgc ggcaagtgc ttccctcag aggttcaat 
cttgagttg catctaagg acgcatacg ggagaacgg 
ccgttccag tgtgaggtt tgcaaaaac ggttacgtt 
tccaaagct agtttgaag attcacatg cggacgcat 
acgaatgag cggccgttt gtgtgcgat ttttgcggg 
aaagctttc agacaatcg ggcgatttg acgagtcat 
aagcgcctc catgggacg gagaaaccg atagaatgc 
caagtctgc cagaaaaga tttacgacc gtaatgaag 
ctcaagtac cacatgagg aaccacacg ggggagcgg 
ccctacgtt tgtaccgtg tgtggtcgg ggcttcacc 
gtgaacacg attttattg cggcacatg agagtgcac 
tcaggggag cgtccatac gtttgcgtg acgtgtggt 
aaagctttt tcgcaatcg agcactctt aacacgcac 
atgaaggtg catgcacca tctccgaaa ttcaccaac 
caagagtcg cagcaaaga attccgaaa ttccacaca 
accaacgaa caaaagttc caaaaccaa caacacccg 
cctccacag caagccccc gaccatagc aatcgttta 
atgcaaacg gataatcgc atgatgcag tcggaaccg 
caacgtatg atgcctcaa gacaacact cggatgatc 
tcgaatgat ggctccagg attataacg gataattca 
caccttatc gccgataat aaaggtttg attaatgat 
gggaccaga ttgttggtt aatatgacg gagccgccg 
cggcttcta gttcacgat aattcgagg tttgtaacg 
aatgataat cggttgttg acgaacgac ggacgcttg 
ctgactaat gttaacgat aattcgagg cttttgacg 
aatgacaat gcgaggcat ttagccgta atcgaaagt 
tcgaggatt ttaaccgat aagtattta gctagttac 
gagccatat catcgaggg catttgtaa
    mRNA sequence
    • >TC013871 RA
      acaatcgac cagtggaag aatctattt tcgccattt 
cctttgcgt gtttgtgca tcttttgtt gtgtttata 
tttgtgatg taatttaac ttttgcgat ggaggttaa 
aatagaacc aagcgtatt cgatataaa taagatatg 
tagggcctg tctctcgga aaaaggcga aatgaggtc 
cgtgttcct gaccgatga atctaccgg acatgctat 
gatacttgc agaaatgat cacgggaat ttcctccgt 
acagattga caacggcga cgggttgcc ctccttggt 
gtgcctcca atgcgtcca ccagctggc tagagccta 
ctccttcaa gcagctttg cgagcagtc cgacaccaa 
tctgcgcca atacctagg cctgccgcc aagtatgaa 
gcagaagaa agaagagga gaccacaca tcccaccga 
ctttgccac aaccctcct tcttgataa tttcggctt 
ggactcgtc atccggtga tagtgacga tgatgatta 
caaaatcga ctacaattt actccagaa ttcgttatc 
cgagaacgc caacgacga aaaagccat cgcccagag 
acaactcct caaggctgc caaggcgca gaagagcaa 
actgtcgaa gaagttgct cgcaaaggc cgggggaaa 
agcagctgt tctggcggc aaaaaaact gaagcgcaa 
caccaacca atgcaacgt gtgcaagaa acagtttat 
taatatgaa gtcgttccg caagcactt gcggacgca 
catcgagga ccgtccgta caaatgcaa gctgtgtcc 
ccggggatt caccgagga aaactacct gaacaacca 
catgcgcac ccacgtgcc tgaagacca gaagccgca 
tgagtgcaa agtgtgcaa aaaacggtt cattcacac 
tacactcct caccaaaca catgctgaa gcacagcgg 
ggagaagcc gttcgtgtg caagatttg caacaaggg 
gtgttatgc cgaaaatag tcttctcaa gcacatgaa 
aatccacga aaagaaaga gggggatcc ggcgttgct 
taaacacat ttgtgacta ctgtaaaac tgaatttcc 
cgatgctca gagtttgtc ggttcatat aaaacagca 
tacggggga tcggccctt tgtgtgcaa catttgcgg 
caagtgctt ccctcagag gttcaatct tgagttgca 
tctaaggac gcatacggg agaacggcc gttccagtg 
tgaggtttg caaaaacgg ttacgtttc caaagctag 
tttgaagat tcacatgcg gacgcatac gaatgagcg 
gccgtttgt gtgcgattt ttgcgggaa agctttcag 
acaatcggg cgatttgac gagtcataa gcgcctcca 
tgggacgga gaaaccgat agaatgcca agtctgcca 
gaaaagatt tacgaccgt aatgaagct caagtacca 
catgaggaa ccacacggg ggagcggcc ctacgtttg 
taccgtgtg tggtcgggg cttcaccgt gaacacgat 
tttattgcg gcacatgag agtgcactc aggggagcg 
tccatacgt ttgcgtgac gtgtggtaa agctttttc 
gcaatcgag cactcttaa cacgcacat gaaggtgca 
tgcaccatc tccgaaatt caccaacca agagtcgca 
gcaaagaat tccgaaatt ccacacaac caacgaaca 
aaagttcca aaaccaaca acacccgcc tccacagca 
agcccccga ccatagcaa tcgtttaat gcaaacgga 
taatcgcat gatgcagtc ggaaccgca acgtatgat 
gcctcaaga caacactcg gatgatctc gaatgatgg 
ctccaggat tataacgga taattcaca ccttatcgc 
cgataataa aggtttgat taatgatgg gaccagatt 
gttggttaa tatgacgga gccgccgcg gcttctagt 
tcacgataa ttcgaggtt tgtaacgaa tgataatcg 
gttgttgac gaacgacgg acgcttgct gactaatgt 
taacgataa ttcgaggct tttgacgaa tgacaatgc 
gaggcattt agccgtaat cgaaagttc gaggatttt 
aaccgataa gtatttagc tagttacga gccatatca 
tcgagggca tttgtaaag tgtcttcgt gcttgcgtt 
tgattacac aattattta tagtgtact ttagaggta 
ataataatt tattggtcg atgtatttt tttaaggga 
agtatcgta tttaataat tggttgatt aaatggtac 
aaaaatttg aagaaatta agttcattt tctaatatt 
ttgaggaaa taatagtta tttttgtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY