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Gene information
General information
TC013871 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2628452..2633257
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013871
    iBeetle screen iB_09975

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    mature egg not present - Number of animals showing the phenotype: 2

    egg chamber mostlynot present - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013871 RA
      MEVKIEPSV FDINKICRA CLSEKGEMR SVFLTDEST 
      GHAMILAEM ITGISSVQI DNGDGLPSL VCLQCVHQL 
      ARAYSFKQL CEQSDTNLR QYLGLPPSM KQKKEEETT 
      HPTDFATTL LLDNFGLDS SSGDSDDDD YKIDYNLLQ 
      NSLSENAND EKAIAQRQL LKAAKAQKS KLSKKLLAK 
      AGGKAAVLA AKKLKRNTN QCNVCKKQF INMKSFRKH 
      LRTHIEDRP YKCKLCPRG FTEENYLNN HMRTHVPED 
      QKPHECKVC KKRFIHTTL LTKHMLKHS GEKPFVCKI 
      CNKGCYAEN SLLKHMKIH EKKEGDPAL LKHICDYCK 
      TEFPDAQSL SVHIKQHTG DRPFVCNIC GKCFPQRFN 
      LELHLRTHT GERPFQCEV CKNGYVSKA SLKIHMRTH 
      TNERPFVCD FCGKAFRQS GDLTSHKRL HGTEKPIEC 
      QVCQKRFTT VMKLKYHMR NHTGERPYV CTVCGRGFT 
      VNTILLRHM RVHSGERPY VCVTCGKAF SQSSTLNTH 
      MKVHAPSPK FTNQESQQR IPKFHTTNE QKFQNQQHP 
      PPQQAPDHS NRLMQTDNR MMQSEPQRM MPQDNTRMI 
      SNDGSRIIT DNSHLIADN KGLINDGTR LLVNMTEPP 
      RLLVHDNSR FVTNDNRLL TNDGRLLTN VNDNSRLLT 
      NDNARHLAV IESSRILTD KYLASYEPY HRGHL
    CDS sequence
    • >TC013871 RA
      atggaggtt aaaatagaa ccaagcgta ttcgatata 
      aataagata tgtagggcc tgtctctcg gaaaaaggc 
      gaaatgagg tccgtgttc ctgaccgat gaatctacc 
      ggacatgct atgatactt gcagaaatg atcacggga 
      atttcctcc gtacagatt gacaacggc gacgggttg 
      ccctccttg gtgtgcctc caatgcgtc caccagctg 
      gctagagcc tactccttc aagcagctt tgcgagcag 
      tccgacacc aatctgcgc caataccta ggcctgccg 
      ccaagtatg aagcagaag aaagaagag gagaccaca 
      catcccacc gactttgcc acaaccctc cttcttgat 
      aatttcggc ttggactcg tcatccggt gatagtgac 
      gatgatgat tacaaaatc gactacaat ttactccag 
      aattcgtta tccgagaac gccaacgac gaaaaagcc 
      atcgcccag agacaactc ctcaaggct gccaaggcg 
      cagaagagc aaactgtcg aagaagttg ctcgcaaag 
      gccggggga aaagcagct gttctggcg gcaaaaaaa 
      ctgaagcgc aacaccaac caatgcaac gtgtgcaag 
      aaacagttt attaatatg aagtcgttc cgcaagcac 
      ttgcggacg cacatcgag gaccgtccg tacaaatgc 
      aagctgtgt ccccgggga ttcaccgag gaaaactac 
      ctgaacaac cacatgcgc acccacgtg cctgaagac 
      cagaagccg catgagtgc aaagtgtgc aaaaaacgg 
      ttcattcac actacactc ctcaccaaa cacatgctg 
      aagcacagc ggggagaag ccgttcgtg tgcaagatt 
      tgcaacaag gggtgttat gccgaaaat agtcttctc 
      aagcacatg aaaatccac gaaaagaaa gagggggat 
      ccggcgttg cttaaacac atttgtgac tactgtaaa 
      actgaattt cccgatgct cagagtttg tcggttcat 
      ataaaacag catacgggg gatcggccc tttgtgtgc 
      aacatttgc ggcaagtgc ttccctcag aggttcaat 
      cttgagttg catctaagg acgcatacg ggagaacgg 
      ccgttccag tgtgaggtt tgcaaaaac ggttacgtt 
      tccaaagct agtttgaag attcacatg cggacgcat 
      acgaatgag cggccgttt gtgtgcgat ttttgcggg 
      aaagctttc agacaatcg ggcgatttg acgagtcat 
      aagcgcctc catgggacg gagaaaccg atagaatgc 
      caagtctgc cagaaaaga tttacgacc gtaatgaag 
      ctcaagtac cacatgagg aaccacacg ggggagcgg 
      ccctacgtt tgtaccgtg tgtggtcgg ggcttcacc 
      gtgaacacg attttattg cggcacatg agagtgcac 
      tcaggggag cgtccatac gtttgcgtg acgtgtggt 
      aaagctttt tcgcaatcg agcactctt aacacgcac 
      atgaaggtg catgcacca tctccgaaa ttcaccaac 
      caagagtcg cagcaaaga attccgaaa ttccacaca 
      accaacgaa caaaagttc caaaaccaa caacacccg 
      cctccacag caagccccc gaccatagc aatcgttta 
      atgcaaacg gataatcgc atgatgcag tcggaaccg 
      caacgtatg atgcctcaa gacaacact cggatgatc 
      tcgaatgat ggctccagg attataacg gataattca 
      caccttatc gccgataat aaaggtttg attaatgat 
      gggaccaga ttgttggtt aatatgacg gagccgccg 
      cggcttcta gttcacgat aattcgagg tttgtaacg 
      aatgataat cggttgttg acgaacgac ggacgcttg 
      ctgactaat gttaacgat aattcgagg cttttgacg 
      aatgacaat gcgaggcat ttagccgta atcgaaagt 
      tcgaggatt ttaaccgat aagtattta gctagttac 
      gagccatat catcgaggg catttgtaa
    mRNA sequence
    • >TC013871 RA
      acaatcgac cagtggaag aatctattt tcgccattt 
      cctttgcgt gtttgtgca tcttttgtt gtgtttata 
      tttgtgatg taatttaac ttttgcgat ggaggttaa 
      aatagaacc aagcgtatt cgatataaa taagatatg 
      tagggcctg tctctcgga aaaaggcga aatgaggtc 
      cgtgttcct gaccgatga atctaccgg acatgctat 
      gatacttgc agaaatgat cacgggaat ttcctccgt 
      acagattga caacggcga cgggttgcc ctccttggt 
      gtgcctcca atgcgtcca ccagctggc tagagccta 
      ctccttcaa gcagctttg cgagcagtc cgacaccaa 
      tctgcgcca atacctagg cctgccgcc aagtatgaa 
      gcagaagaa agaagagga gaccacaca tcccaccga 
      ctttgccac aaccctcct tcttgataa tttcggctt 
      ggactcgtc atccggtga tagtgacga tgatgatta 
      caaaatcga ctacaattt actccagaa ttcgttatc 
      cgagaacgc caacgacga aaaagccat cgcccagag 
      acaactcct caaggctgc caaggcgca gaagagcaa 
      actgtcgaa gaagttgct cgcaaaggc cgggggaaa 
      agcagctgt tctggcggc aaaaaaact gaagcgcaa 
      caccaacca atgcaacgt gtgcaagaa acagtttat 
      taatatgaa gtcgttccg caagcactt gcggacgca 
      catcgagga ccgtccgta caaatgcaa gctgtgtcc 
      ccggggatt caccgagga aaactacct gaacaacca 
      catgcgcac ccacgtgcc tgaagacca gaagccgca 
      tgagtgcaa agtgtgcaa aaaacggtt cattcacac 
      tacactcct caccaaaca catgctgaa gcacagcgg 
      ggagaagcc gttcgtgtg caagatttg caacaaggg 
      gtgttatgc cgaaaatag tcttctcaa gcacatgaa 
      aatccacga aaagaaaga gggggatcc ggcgttgct 
      taaacacat ttgtgacta ctgtaaaac tgaatttcc 
      cgatgctca gagtttgtc ggttcatat aaaacagca 
      tacggggga tcggccctt tgtgtgcaa catttgcgg 
      caagtgctt ccctcagag gttcaatct tgagttgca 
      tctaaggac gcatacggg agaacggcc gttccagtg 
      tgaggtttg caaaaacgg ttacgtttc caaagctag 
      tttgaagat tcacatgcg gacgcatac gaatgagcg 
      gccgtttgt gtgcgattt ttgcgggaa agctttcag 
      acaatcggg cgatttgac gagtcataa gcgcctcca 
      tgggacgga gaaaccgat agaatgcca agtctgcca 
      gaaaagatt tacgaccgt aatgaagct caagtacca 
      catgaggaa ccacacggg ggagcggcc ctacgtttg 
      taccgtgtg tggtcgggg cttcaccgt gaacacgat 
      tttattgcg gcacatgag agtgcactc aggggagcg 
      tccatacgt ttgcgtgac gtgtggtaa agctttttc 
      gcaatcgag cactcttaa cacgcacat gaaggtgca 
      tgcaccatc tccgaaatt caccaacca agagtcgca 
      gcaaagaat tccgaaatt ccacacaac caacgaaca 
      aaagttcca aaaccaaca acacccgcc tccacagca 
      agcccccga ccatagcaa tcgtttaat gcaaacgga 
      taatcgcat gatgcagtc ggaaccgca acgtatgat 
      gcctcaaga caacactcg gatgatctc gaatgatgg 
      ctccaggat tataacgga taattcaca ccttatcgc 
      cgataataa aggtttgat taatgatgg gaccagatt 
      gttggttaa tatgacgga gccgccgcg gcttctagt 
      tcacgataa ttcgaggtt tgtaacgaa tgataatcg 
      gttgttgac gaacgacgg acgcttgct gactaatgt 
      taacgataa ttcgaggct tttgacgaa tgacaatgc 
      gaggcattt agccgtaat cgaaagttc gaggatttt 
      aaccgataa gtatttagc tagttacga gccatatca 
      tcgagggca tttgtaaag tgtcttcgt gcttgcgtt 
      tgattacac aattattta tagtgtact ttagaggta 
      ataataatt tattggtcg atgtatttt tttaaggga 
      agtatcgta tttaataat tggttgatt aaatggtac 
      aaaaatttg aagaaatta agttcattt tctaatatt 
      ttgaggaaa taatagtta tttttgtaa
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