Status
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Gene information
General information
TC013938 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2612279..2615212
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013938
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09974

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013938 RA
      MPARYFFIQ AVNSENQSI SSDLGAIFD IHFEGVTKT 
      QKPCRIWAN SLNRKDGSY IIRYKLYEP CFSLKINIL 
      LNKEHVDKS PYEIPEPVY PDDCDCPVG DIAQFLEKW 
      ECGNTPRQI LADLKPFQT VNWDKLRDK VIKKFDQPH 
      SISLCRYVI KNNEIYRTC YGKYVGFKM FMDAILLSL 
      SRKVNLPDL EFFINLGDW PLVTEKIET FPIFSWCGS 
      TTSLDIVMP TYDITESTL ENMGRVMLD MLSVQGNVK 
      ESWENRTGQ AFWRGRDSN QHRLDLIDI ARKHPDLFN 
      VSLTNFFFF RDKQDVYGP KSEHVSFFS FFDYKYQLA 
      LDGTVAAYR FPYLLAGGS LVIKQESQY YEHFYNDLI 
      PNTHYILMK RDLSDLVAK LQWSIQNDK EAQIIASNG 
      QKFANENLL PQHIFCYHA HLLHQFGTR IESEVNILN 
      NMEKVEQIK QQSYCDCKS KVKDEL
    • >TC013938 RB
      MPARYFFIQ AVNSENQSI SSDLGAIFD IHFEGVTKT 
      QKPCRIWAN SLNRKDGSY IIRYKLYEP CFSLKINIL 
      LNKEHVDKS PYEIPEPVY PDDCDCPVG DIAQFLEKW 
      ECGNTPRQI LADLKPFQT VNWDKLRDK VIKKFDQPH 
      SISLCRYVI KNNEIYRTC YGKYVGFKM FMDAILLSL 
      SRKVNLPDL EFFINLGDW PLVTEKIET FPIFSWCGS 
      TTSLDIVMP TYDITESTL ENMGRVMLD MLSVQGNVK 
      ESWENRTGQ AFWRGRDSN QHRLDLIDI ARKHPDLFN 
      VSLTNFFFF RDKQDVYGP KSEHVSFFS FFDYKYQLA 
      LDGTVAAYR FPYLLAGGS LVIKQESQY YEHFYNDLI 
      PNTHYILMK RDLSDLVAK LQWSIQNDK EAQIIASNG 
      QKFANENLL PQHIFCYHA HLLHQFGKR
    CDS sequence
    • >TC013938 RA
      atgccggcg aggtatttt tttattcaa gccgtaaat 
      tcagaaaat caaagtatt tcctcagac ttgggggca 
      atcttcgac atacacttt gaaggtgta accaagaca 
      cagaagcct tgccgcatt tgggcaaac agtctcaat 
      aggaaagac ggttcctac attattaga tataagttg 
      tatgagccg tgcttcagc ttaaaaatt aatatactc 
      ctcaataag gaacatgtg gacaagtct ccttatgaa 
      attccagaa cctgtttat ccagacgat tgtgattgt 
      ccagttggt gatattgcc caatttttg gaaaaatgg 
      gaatgtggc aacacccct cggcaaatt ctcgccgac 
      ctgaagccc ttccaaaca gttaattgg gataaactt 
      agggataaa gtaattaaa aaattcgac cagccgcac 
      tcaatcagt ttgtgccgc tacgtcata aaaaataat 
      gaaatttac cgtacttgt tacggaaaa tacgtaggt 
      tttaagatg ttcatggac gcgattttg ttatccttg 
      agccgaaaa gtgaactta cccgacctg gaatttttc 
      attaatttg ggcgactgg cctttggtg actgaaaaa 
      atcgaaaca tttccaatt ttttcgtgg tgcggaagc 
      accacaagt ctggacatt gttatgcca acgtatgat 
      attaccgaa tcaacacta gaaaatatg ggtcgagtg 
      atgttagat atgctctct gtccaagga aatgtgaaa 
      gaatcgtgg gaaaataga acagggcaa gcgttttgg 
      cgaggaaga gactcaaat cagcacaga cttgatctg 
      attgacatt gcgaggaaa catcccgat ttgttcaac 
      gtttcgttg acgaatttc ttcttcttc agggacaaa 
      caagacgtc tatggcccc aaatcggaa cacgtctca 
      ttttttagt ttctttgat tataaatat caactagct 
      ctagatggt acagtcgcc gcttaccgt tttccgtat 
      ctcttagct ggaggctct ctagtcata aaacaagaa 
      tctcagtat tatgagcat ttttacaac gacttaatc 
      ccgaatact cattatatt ttaatgaaa cgagactta 
      tcagacttg gttgcgaag ctccagtgg tccattcag 
      aacgacaaa gaagcacaa ataatcgcc tctaatggt 
      caaaaattc gccaatgaa aatctatta ccacaacac 
      attttttgc tatcacgcc catctattg catcagttt 
      ggtacaaga atcgaaagt gaagtcaac attttaaac 
      aatatggaa aaagttgaa caaataaaa caacaaagt 
      tactgtgat tgtaaatcg aaggtaaaa gatgaatta 
      taa
    • >TC013938 RB
      atgccggcg aggtatttt tttattcaa gccgtaaat 
      tcagaaaat caaagtatt tcctcagac ttgggggca 
      atcttcgac atacacttt gaaggtgta accaagaca 
      cagaagcct tgccgcatt tgggcaaac agtctcaat 
      aggaaagac ggttcctac attattaga tataagttg 
      tatgagccg tgcttcagc ttaaaaatt aatatactc 
      ctcaataag gaacatgtg gacaagtct ccttatgaa 
      attccagaa cctgtttat ccagacgat tgtgattgt 
      ccagttggt gatattgcc caatttttg gaaaaatgg 
      gaatgtggc aacacccct cggcaaatt ctcgccgac 
      ctgaagccc ttccaaaca gttaattgg gataaactt 
      agggataaa gtaattaaa aaattcgac cagccgcac 
      tcaatcagt ttgtgccgc tacgtcata aaaaataat 
      gaaatttac cgtacttgt tacggaaaa tacgtaggt 
      tttaagatg ttcatggac gcgattttg ttatccttg 
      agccgaaaa gtgaactta cccgacctg gaatttttc 
      attaatttg ggcgactgg cctttggtg actgaaaaa 
      atcgaaaca tttccaatt ttttcgtgg tgcggaagc 
      accacaagt ctggacatt gttatgcca acgtatgat 
      attaccgaa tcaacacta gaaaatatg ggtcgagtg 
      atgttagat atgctctct gtccaagga aatgtgaaa 
      gaatcgtgg gaaaataga acagggcaa gcgttttgg 
      cgaggaaga gactcaaat cagcacaga cttgatctg 
      attgacatt gcgaggaaa catcccgat ttgttcaac 
      gtttcgttg acgaatttc ttcttcttc agggacaaa 
      caagacgtc tatggcccc aaatcggaa cacgtctca 
      ttttttagt ttctttgat tataaatat caactagct 
      ctagatggt acagtcgcc gcttaccgt tttccgtat 
      ctcttagct ggaggctct ctagtcata aaacaagaa 
      tctcagtat tatgagcat ttttacaac gacttaatc 
      ccgaatact cattatatt ttaatgaaa cgagactta 
      tcagacttg gttgcgaag ctccagtgg tccattcag 
      aacgacaaa gaagcacaa ataatcgcc tctaatggt 
      caaaaattc gccaatgaa aatctatta ccacaacac 
      attttttgc tatcacgcc catctattg catcagttt 
      ggtaaaaga tga
    mRNA sequence
    • >TC013938 RA
      ttgttaacc tcaaaaatg tgcaaattt atttatttt 
      tattttcca tttatcgta ctgttttct cacgtaagg 
      agcgaatta attgttagt gccaaatta tcaaaagta 
      tgggggcct ggactcaaa cctcacatt tttacaatg 
      ccggcgagg tattttttt attcaagcc gtaaattca 
      gaaaatcaa agtatttcc tcagacttg ggggcaatc 
      ttcgacata cactttgaa ggtgtaacc aagacacag 
      aagccttgc cgcatttgg gcaaacagt ctcaatagg 
      aaagacggt tcctacatt attagatat aagttgtat 
      gagccgtgc ttcagctta aaaattaat atactcctc 
      aataaggaa catgtggac aagtctcct tatgaaatt 
      ccagaacct gtttatcca gacgattgt gattgtcca 
      gttggtgat attgcccaa tttttggaa aaatgggaa 
      tgtggcaac acccctcgg caaattctc gccgacctg 
      aagcccttc caaacagtt aattgggat aaacttagg 
      gataaagta attaaaaaa ttcgaccag ccgcactca 
      atcagtttg tgccgctac gtcataaaa aataatgaa 
      atttaccgt acttgttac ggaaaatac gtaggtttt 
      aagatgttc atggacgcg attttgtta tccttgagc 
      cgaaaagtg aacttaccc gacctggaa tttttcatt 
      aatttgggc gactggcct ttggtgact gaaaaaatc 
      gaaacattt ccaattttt tcgtggtgc ggaagcacc 
      acaagtctg gacattgtt atgccaacg tatgatatt 
      accgaatca acactagaa aatatgggt cgagtgatg 
      ttagatatg ctctctgtc caaggaaat gtgaaagaa 
      tcgtgggaa aatagaaca gggcaagcg ttttggcga 
      ggaagagac tcaaatcag cacagactt gatctgatt 
      gacattgcg aggaaacat cccgatttg ttcaacgtt 
      tcgttgacg aatttcttc ttcttcagg gacaaacaa 
      gacgtctat ggccccaaa tcggaacac gtctcattt 
      tttagtttc tttgattat aaatatcaa ctagctcta 
      gatggtaca gtcgccgct taccgtttt ccgtatctc 
      ttagctgga ggctctcta gtcataaaa caagaatct 
      cagtattat gagcatttt tacaacgac ttaatcccg 
      aatactcat tatatttta atgaaacga gacttatca 
      gacttggtt gcgaagctc cagtggtcc attcagaac 
      gacaaagaa gcacaaata atcgcctct aatggtcaa 
      aaattcgcc aatgaaaat ctattacca caacacatt 
      ttttgctat cacgcccat ctattgcat cagtttggt 
      acaagaatc gaaagtgaa gtcaacatt ttaaacaat 
      atggaaaaa gttgaacaa ataaaacaa caaagttac 
      tgtgattgt aaatcgaag gtaaaagat gaattataa 
      ttaatgttg caatacaga accacaatg aattgttac 
      aataaataa attaaaagc tcttactat atccaaact 
      tgtctacat ttattaaaa ttgattcta gctcatgta 
      gtagctccc ttttgactg aatcaggca ccatactcc 
      tagcttttg gcgttactg tagctatga gctcgtcta 
      cagtgggta tgtgtttct cattttccg tgatggcct 
      tcctgcaag ctagcctga cttggtcgc gccatccac 
      actcgatta agcgtgtct gaaccaatt cagttaccc 
      tgaaataaa tcgatgttt cctcccttt aaatttaaa 
      tgccacaca cctgtccaa accgtgatt aatatccaa 
      gtgcaaatt aattttcgc atcgtattc ttgtgacat 
      tttcacacc aaaactctt caatatcct tcctgtcaa 
      ttatttttt agaaaaaca tgcaaaatg tgtttgttt 
      ttatcttgg atatttccg cagatgaag acattgaac 
      tgtcacctc accttctgt cacctgtca aattttgta 
      attaattaa aaccatatt ttcaaa
    • >TC013938 RB
      ttgttaacc tcaaaaatg tgcaaattt atttatttt 
      tattttcca tttatcgta ctgttttct cacgtaagg 
      agcgaatta attgttagt gccaaatta tcaaaagta 
      tgggggcct ggactcaaa cctcacatt tttacaatg 
      ccggcgagg tattttttt attcaagcc gtaaattca 
      gaaaatcaa agtatttcc tcagacttg ggggcaatc 
      ttcgacata cactttgaa ggtgtaacc aagacacag 
      aagccttgc cgcatttgg gcaaacagt ctcaatagg 
      aaagacggt tcctacatt attagatat aagttgtat 
      gagccgtgc ttcagctta aaaattaat atactcctc 
      aataaggaa catgtggac aagtctcct tatgaaatt 
      ccagaacct gtttatcca gacgattgt gattgtcca 
      gttggtgat attgcccaa tttttggaa aaatgggaa 
      tgtggcaac acccctcgg caaattctc gccgacctg 
      aagcccttc caaacagtt aattgggat aaacttagg 
      gataaagta attaaaaaa ttcgaccag ccgcactca 
      atcagtttg tgccgctac gtcataaaa aataatgaa 
      atttaccgt acttgttac ggaaaatac gtaggtttt 
      aagatgttc atggacgcg attttgtta tccttgagc 
      cgaaaagtg aacttaccc gacctggaa tttttcatt 
      aatttgggc gactggcct ttggtgact gaaaaaatc 
      gaaacattt ccaattttt tcgtggtgc ggaagcacc 
      acaagtctg gacattgtt atgccaacg tatgatatt 
      accgaatca acactagaa aatatgggt cgagtgatg 
      ttagatatg ctctctgtc caaggaaat gtgaaagaa 
      tcgtgggaa aatagaaca gggcaagcg ttttggcga 
      ggaagagac tcaaatcag cacagactt gatctgatt 
      gacattgcg aggaaacat cccgatttg ttcaacgtt 
      tcgttgacg aatttcttc ttcttcagg gacaaacaa 
      gacgtctat ggccccaaa tcggaacac gtctcattt 
      tttagtttc tttgattat aaatatcaa ctagctcta 
      gatggtaca gtcgccgct taccgtttt ccgtatctc 
      ttagctgga ggctctcta gtcataaaa caagaatct 
      cagtattat gagcatttt tacaacgac ttaatcccg 
      aatactcat tatatttta atgaaacga gacttatca 
      gacttggtt gcgaagctc cagtggtcc attcagaac 
      gacaaagaa gcacaaata atcgcctct aatggtcaa 
      aaattcgcc aatgaaaat ctattacca caacacatt 
      ttttgctat cacgcccat ctattgcat cagtttggt 
      aaaagatga attataatt aatgttgca atacagaac 
      cacaatgaa ttgttacaa taaataaat taaaagctc 
      ttactatat ccaaacttg tctacattt attaaaatt 
      gattctagc tcatgtagt agctccctt ttgactgaa 
      tcaggcacc atactccta gcttttggc gttactgta 
      gctatgagc tcgtctaca gtgggtatg tgtttctca 
      ttttccgtg atggccttc ctgcaagct agcctgact 
      tggtcgcgc catccacac tcgattaag cgtgtctga 
      accaattca gttaccctg aaataaatc gatgtttcc 
      tccctttaa atttaaatg ccacacacc tgtccaaac 
      cgtgattaa tatccaagt gcaaattaa ttttcgcat 
      cgtattctt gtgacattt tcacaccaa aactcttca 
      atatccttc ctgtcaatt attttttag aaaaacatg 
      caaaatgtg tttgttttt atcttggat atttccgca 
      gatgaagac attgaactg tcacctcac cttctgtca 
      cctgtcaaa ttttgtaat taattaaaa ccatatttt 
      caaaattta aatttaatt aaaatgtac tggtattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY