Status
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Gene information
General information
TC013883 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2160080..2168404
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013883
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09971

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013883 RA
      MGEMAVGDR PPSPARLSH TSDKHPKNT LSELNVLRR 
      HRELCDVVL NVGTRKIFA HRVILSACS PYFRAMFTG 
      ELAESRQTE VTIRDIDEI AMDLLIDFC YTSHIIVEE 
      SNVQMLLPA ACLLQLTEI QDICCEFLK RQLDPTNCL 
      GIRAFADTH SCRELLRIA DKFTQHNFQ EVMESEEFL 
      LLPVGQLVD IISSDELNV RTEEQVFNA VMSWVKYNV 
      TERRQHLHQ VLQHVRLPL LSPKFLVGT VGSDLLVRS 
      DESCRDLVD EAKNYLLLP QERPLMQGP RTRPRKPTR 
      RGEVLFAVG GWCSGDAIA SVEKFDPQT MEWKMVAPM 
      SKRRCGVGV AVLNDLLYA VGGHDGQSY LNSIERYDP 
      QTDQWSCDV APTTSCRTS VGVAVLDNL LYAVGGQDG 
      VQCLNHVER YDPKENKWT KVAPMTTRR LGVAVAVLG 
      GYLYAIGGS DGQSPLNTV ERYDPRHNK WALVSPMST 
      RRKHLGCAV FNNLIYAVG GRDDCMELS SAERYNPHT 
      NTWSPIVAM TSRRSGVGL AVVNGQLYA VGGFDGTAY 
      LKTIEFYDT EQNQWRLCG SMNYRRLGG GVGVMRAPQ 
      TENRIW
    CDS sequence
    • >TC013883 RA
      atgggggag atggcagtt ggagaccgc ccaccttct 
      ccagcaagg ctgtctcac acgtctgac aagcacccc 
      aaaaacact ctttccgag ctcaatgtc ctgcggagg 
      caccgggag ctgtgcgac gtggtgctc aacgtgggc 
      acgcgcaag attttcgcc catcgggtc attctgagc 
      gcttgcagc ccctacttc cgggccatg ttcaccggg 
      gagttggcg gagtcccgc cagactgaa gtcacgatt 
      cgggacatt gacgagatt gcgatggat ttgttgatt 
      gatttttgc tacacctcg cacatcatc gtcgaggag 
      tccaacgtg caaatgctg ctcccggca gcttgtctc 
      ctgcagctg actgaaatc caggacatt tgctgcgag 
      tttctgaaa cgtcagttg gacccaact aactgtctt 
      gggattcga gcctttgcc gacacgcac tcctgtcgc 
      gaattgctc cgaatcgcc gataaattc acccaacac 
      aacttccaa gaagtgatg gaaagtgag gagtttttg 
      cttctgccg gttggacaa ttagttgat attatctca 
      agtgatgag ttgaacgtg cgcactgaa gagcaagtc 
      ttcaacgct gttatgtcc tgggtcaag tacaatgtg 
      actgaaagg cggcaacac ctccatcag gtgttacaa 
      cacgttaga ttgcctttg ctcagtcct aagtttcta 
      gtaggcact gtggggtcg gatttgctg gttaggtcc 
      gatgaatcg tgtcgggat ctcgttgat gaggctaaa 
      aattatctt ttactgcca caagaacgg ccgttgatg 
      cagggcccc agaaccagg ccgaggaaa ccaacgaga 
      agaggagag gttttattt gcagtcggg ggatggtgt 
      agcggggat gctatcgcc tccgtggag aagtttgac 
      ccgcagacg atggaatgg aagatggtg gcgccgatg 
      agcaagagg cggtgtgga gtgggagtc gctgttttg 
      aatgatttg ttgtatgcg gttggcgga catgatggg 
      cagagctat ctcaacagt attgaaagg tacgacccc 
      caaaccgac cagtggtca tgtgacgtg gcccctacg 
      acctcctgt cgaacaagt gtgggcgta gccgttctc 
      gacaacctt ctctacgct gtcgggggt caggacggg 
      gtccagtgt ttgaatcac gtcgaaaga tacgatcct 
      aaagaaaac aaatggacc aaagtagcc cccatgacg 
      acgagacgt ttaggcgtc gccgttgcc gtcttaggt 
      ggttatttg tacgcaata gggggctcc gatggacaa 
      tcgccacta aatacagta gaacgatac gatccaaga 
      cacaacaaa tgggctcta gtgagccct atgtcaaca 
      cgaagaaaa catttaggc tgtgctgtc ttcaataat 
      ttaatttat gcggtgggc ggccgcgac gactgcatg 
      gaattgtca agtgccgaa cggtataat cctcacacg 
      aacacttgg agtcccatt gtggcgatg acgtccaga 
      aggagtggg gtcggttta gcggtggtc aacggccag 
      ttgtacgca gtaggaggc ttcgatggt acggcctat 
      ttaaaaaca attgaattc tacgatact gaacaaaac 
      caatggaga ttatgcggt tctatgaat tatagaagg 
      ttaggaggc ggagttggc gttatgaga gctccacaa 
      actgaaaat cggatatgg tag
    mRNA sequence
    • >TC013883 RA
      tcaagtcaa acgtcaata cggtttgtg tattttgcg 
      aaaatcaga tgggttttc ttggtgaaa taatgtgct 
      aaaagcgcg tgattgtgc cactcgact ggcagccga 
      gtttagctt tcgccccac gtgccgcaa aaaggaccg 
      aagatagac tccgtgtgg ggttgggcg tctccgccc 
      ttgcgcctc caactggcc acccacatt cgaggcact 
      tgcagaatg ggggagatg gcagttgga gaccgccca 
      ccttctcca gcaaggctg tctcacacg tctgacaag 
      caccccaaa aacactctt tccgagctc aatgtcctg 
      cggaggcac cgggagctg tgcgacgtg gtgctcaac 
      gtgggcacg cgcaagatt ttcgcccat cgggtcatt 
      ctgagcgct tgcagcccc tacttccgg gccatgttc 
      accggggag ttggcggag tcccgccag actgaagtc 
      acgattcgg gacattgac gagattgcg atggatttg 
      ttgattgat ttttgctac acctcgcac atcatcgtc 
      gaggagtcc aacgtgcaa atgctgctc ccggcagct 
      tgtctcctg cagctgact gaaatccag gacatttgc 
      tgcgagttt ctgaaacgt cagttggac ccaactaac 
      tgtcttggg attcgagcc tttgccgac acgcactcc 
      tgtcgcgaa ttgctccga atcgccgat aaattcacc 
      caacacaac ttccaagaa gtgatggaa agtgaggag 
      tttttgctt ctgccggtt ggacaatta gttgatatt 
      atctcaagt gatgagttg aacgtgcgc actgaagag 
      caagtcttc aacgctgtt atgtcctgg gtcaagtac 
      aatgtgact gaaaggcgg caacacctc catcaggtg 
      ttacaacac gttagattg cctttgctc agtcctaag 
      tttctagta ggcactgtg gggtcggat ttgctggtt 
      aggtccgat gaatcgtgt cgggatctc gttgatgag 
      gctaaaaat tatctttta ctgccacaa gaacggccg 
      ttgatgcag ggccccaga accaggccg aggaaacca 
      acgagaaga ggagaggtt ttatttgca gtcggggga 
      tggtgtagc ggggatgct atcgcctcc gtggagaag 
      tttgacccg cagacgatg gaatggaag atggtggcg 
      ccgatgagc aagaggcgg tgtggagtg ggagtcgct 
      gttttgaat gatttgttg tatgcggtt ggcggacat 
      gatgggcag agctatctc aacagtatt gaaaggtac 
      gacccccaa accgaccag tggtcatgt gacgtggcc 
      cctacgacc tcctgtcga acaagtgtg ggcgtagcc 
      gttctcgac aaccttctc tacgctgtc gggggtcag 
      gacggggtc cagtgtttg aatcacgtc gaaagatac 
      gatcctaaa gaaaacaaa tggaccaaa gtagccccc 
      atgacgacg agacgttta ggcgtcgcc gttgccgtc 
      ttaggtggt tatttgtac gcaataggg ggctccgat 
      ggacaatcg ccactaaat acagtagaa cgatacgat 
      ccaagacac aacaaatgg gctctagtg agccctatg 
      tcaacacga agaaaacat ttaggctgt gctgtcttc 
      aataattta atttatgcg gtgggcggc cgcgacgac 
      tgcatggaa ttgtcaagt gccgaacgg tataatcct 
      cacacgaac acttggagt cccattgtg gcgatgacg 
      tccagaagg agtggggtc ggtttagcg gtggtcaac 
      ggccagttg tacgcagta ggaggcttc gatggtacg 
      gcctattta aaaacaatt gaattctac gatactgaa 
      caaaaccaa tggagatta tgcggttct atgaattat 
      agaaggtta ggaggcgga gttggcgtt atgagagct 
      ccacaaact gaaaatcgg atatggtag ttaaggcct 
      aaggaaaaa gaaacgtat tttgtgtgg ttgcctttt 
      gctcacaat tttgtataa tcgaagcat ttttaaaac 
      aattaatac aaaattggg aggtaaaga tttgtgatt 
      tgtattata tttagcgtt tcttttcgc gatctctgt 
      cttctagga aagttgcaa tttctttaa ccgcatttt 
      gttcaggtt agcagcgta acaaacaga aatgctcga 
      atattttct gttttaaac ctttgcata agctgccag 
      ttttcataa ttttattca gaaagtcta agtttgtga 
      ccaattgat ccaaaaatg cgctgtggc cattacctt 
      taaagtctt aaacggact gtacagtgt cattagaaa 
      atgtcaaac tctcttatg gtgacaaaa gacgttctg 
      ttatcaatc gttcagtat cagttgttg ctttttttt 
      cctatgatg tcgaatttg gaagttttt taaataaaa 
      tgtgacgtt ggcgttttt atagttttg aaacaacta 
      atcataaga tattaattg gtgtttttt ttcaacaag 
      aatctgttt aatgtagca agagttgtt ttttccgtt 
      aactaaaga caaaaaata tgatttgaa acagcaaaa 
      acaacttaa gaatttaac tttgaaaag ctatcccgt 
      gaagtttta atttaattt gatgttctg tagagagat 
      tgtcggaca cttttgaat caacttgta tattctgtt 
      tccagcctt tatagcaaa aacaatttt ttaaattac 
      ttttcctgc ttaattaat gcattcttt tgacaataa 
      atttgtcac tagtctagt gcaacacaa ttcatttgg 
      ctattttat aatagaact tctaatttg ttcaaaatt 
      ggttacatt tagacctga cccagttgt acatagtta 
      agcctctaa ttagttaaa ttatttatt ttatactta 
      agtttaatg aaatatact catttaaaa attccacac 
      ctgcaggta ccatggatt tctgagcaa aatgtcact 
      gtaattaag ttggcacaa ttaaactac aaaagtctg 
      acgtttgga tttagtgtt gccaactta atttcaaag 
      tcagaggct tggtataca attagtttt agaaagctt 
      ctcaataga aagtactgc aattgcagt cactgataa 
      aattcatgt taaatgtta tacttctaa gtacctaat 
      tgttgattc ttaaacttt tattcttta ttttcccaa 
      atcaaaact tgactcatt gtttacaaa aggtaaatg 
      taatgtttt gttgactgc tttttcttt tatatttta 
      cgaaacaat aaactgtta taaaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY