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Gene information
General information
TC010940 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1531166..1532316
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010940
    iBeetle screen iB_09968

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010940 RA
      MKISAAISL GLLLAVLAP STQKHIAPR IIGGDEAKA 
      GQFPYAAAI YIKKELSTT FCGGALLSN LWVLTAGHC 
      VDGAVSTIV YLGSTTLQD HDPNRIMLT TDNHVIHPL 
      YNRTTLEHD IGLIMFRMA ITFSDYIKP TSFFPIADL 
      PGGAPVTTF GWGQIDDEH AGVTNHLRY VQLSALTND 
      ECKLVFGSR ITENMVCVS GNYNEGICR GDSGSPLIQ 
      NVGGGHTLI VGVGSFLSE NGCESTDPS GFTRVFPYR 
      DWIRNVTNI V
    CDS sequence
    • >TC010940 RA
      atgaagatt tcggcggca atttctttg ggactcctc 
      ttggcggtg ttggcccca tccacccaa aaacacatc 
      gcacctcgt atcattgga ggagacgaa gcaaaagct 
      ggtcaattt ccatacgct gctgcaatt tatatcaaa 
      aaagaactt agtaccact ttctgtggc ggagctctt 
      cttagcaat ttgtgggtt ttgactgcc ggtcattgc 
      gtcgatggg gccgtttct accattgtt tatttgggt 
      tccactact ttgcaagac catgatcct aacagaatc 
      atgttgacc acagataat cacgtaatt cacccgttg 
      tacaacagg actaccctg gaacacgac attgggctt 
      atcatgttc agaatggcc ataacattc tctgactac 
      attaagccc accagcttc ttccccatc gcagacttg 
      cctggagga gctcctgtt acaaccttt gggtgggga 
      cagatcgat gatgaacat gctggtgtc acaaatcac 
      ttgcgttat gtccagtta agtgcattg acaaatgat 
      gaatgtaaa ctggtcttt ggttctaga attacagaa 
      aacatggtt tgcgtttct ggaaattac aatgaaggg 
      atttgcagg ggtgacagt ggaagtcct ctaatccaa 
      aacgttggt ggtggtcat accctcatt gtaggtgtg 
      ggaagcttt ttaagcgaa aatggatgt gaatctact 
      gatccctct ggatttaca agagttttc ccatacagg 
      gactggata agaaatgtt accaacatt gtgtaa
    mRNA sequence
    • >TC010940 RA
      acattaggt caacatgaa gatttcggc ggcaatttc 
      tttgggact cctcttggc ggtgttggc cccatccac 
      ccaaaaaca catcgcacc tcgtatcat tggaggaga 
      cgaagcaaa agctggtca atttccata cgctgctgc 
      aatttatat caaaaaaga acttagtac cactttctg 
      tggcggagc tcttcttag caatttgtg ggttttgac 
      tgccggtca ttgcgtcga tggggccgt ttctaccat 
      tgtttattt gggttccac tactttgca agaccatga 
      tcctaacag aatcatgtt gaccacaga taatcacgt 
      aattcaccc gttgtacaa caggactac cctggaaca 
      cgacattgg gcttatcat gttcagaat ggccataac 
      attctctga ctacattaa gcccaccag cttcttccc 
      catcgcaga cttgcctgg aggagctcc tgttacaac 
      ctttgggtg gggacagat cgatgatga acatgctgg 
      tgtcacaaa tcacttgcg ttatgtcca gttaagtgc 
      attgacaaa tgatgaatg taaactggt ctttggttc 
      tagaattac agaaaacat ggtttgcgt ttctggaaa 
      ttacaatga agggatttg caggggtga cagtggaag 
      tcctctaat ccaaaacgt tggtggtgg tcataccct 
      cattgtagg tgtgggaag ctttttaag cgaaaatgg 
      atgtgaatc tactgatcc ctctggatt tacaagagt 
      tttcccata cagggactg gataagaaa tgttaccaa 
      cattgtgta aatttttaa gatctaaaa ctgagatta 
      ataaagtta tggaaacat g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY