Status
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Gene information
General information
TC010945 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1645811..1650098
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010945
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09967

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010945 RA
      MDAETINVS KPFEIESQL DLKKDDRNT NSAVKHKKQ 
      SENEALYQT QYENTLDPD CILQINKRL IRVEYPGEV 
      KNVNKALET LGGIEHIET SVADHGRKL ELHFHPENK 
      YNKGCVADK DFKPGILIK VKKKFLQPE PPSDDISYD 
      VEGVSVINF SFNRLCDFQ YLPLVSKEH ETKSSKTEY 
      IYEKIVPHE LPTSEWLES EEAQNMEQF LLPVQFSPY 
      DSSQKKLNV ESDKNDPKL KLKGLTCAE SKVSSKEQK 
      NVCRIFGKN RQQTSIFVT FQDKEIPTG PLESALKLL 
      KHRNLEERI ERVKQLFEE RPIWTKAAI RYNTGLSDE 
      HSKIILPVV AYYFSNGPW RISWVKFGY DPRKDPKAR 
      IYQTLDYRI RTAESTKLK VTAKRNYAG KTSDFLGST 
      NVKKISLKH DMSAKPKME LDERYYVLR PLTIPPARQ 
      MFYQLCDLI LPQIQEKIS RLPKCSGGY CDSKNGWLP 
      DNFIRDCRK IVNDHVLEA VQRELLEDK KFLQEKTSK 
      EKERKKESG SNLDYYNQM LSNIKKGIY KNVGLQPAY 
      VPKDFDPGP SSELISLSS DEENELTVG EIIQSSIPE 
      KVDEGEEEE QSISESSDE AEIDMEAVQ EINEMISQF 
      NVKM
    CDS sequence
    • >TC010945 RA
      atggatgct gaaacaata aacgtttca aaaccattt 
      gaaatagag tcacaactc gacctgaaa aaggacgat 
      aggaacacc aactctgca gtcaagcac aagaaacag 
      tcggaaaat gaggctttg tatcaaaca cagtatgaa 
      aatactctt gatcctgat tgcattttg caaataaat 
      aaacgctta attcgagtt gagtatccc ggggaagtg 
      aaaaatgtc aataaggcg ttggagact ttgggagga 
      atagaacac attgaaacg agtgtggct gatcatgga 
      cgtaaacta gaattacac tttcacccc gaaaacaag 
      tacaataaa ggctgtgtt gcggataag gattttaaa 
      ccaggaatt ttaatcaaa gtgaaaaag aagttccta 
      caacctgaa ccaccatca gatgacatt agttacgat 
      gtggaaggg gtttctgtg ataaacttc agcttcaac 
      aggctttgt gacttccag tacttacct ctagtgtca 
      aaagaacat gaaaccaag tcgtctaaa acagagtac 
      atttatgag aaaattgtt ccacatgaa ttgccaact 
      tctgaatgg cttgaaagc gaagaggca caaaatatg 
      gaacagttt ttacttcca gttcagttt tcaccttac 
      gacagttct caaaaaaaa ctgaatgtc gaaagtgat 
      aaaaacgac cctaaattg aaactcaag ggccttact 
      tgtgccgaa tctaaagtc agttcaaag gagcagaaa 
      aatgtttgc aggatattc gggaaaaat aggcaacaa 
      acgtcaatt tttgttact tttcaagac aaggaaata 
      ccgacgggg cccttagaa agcgctttg aaacttctg 
      aagcacagg aatttggaa gaacgcatt gagagggtc 
      aaacaattg tttgaggag aggcccatc tggacgaag 
      gccgccatt cgatacaac acaggtctc tcagacgaa 
      cattccaaa ataattctc ccagtcgtt gcttattat 
      tttagcaac ggcccttgg cgaatttcc tgggtcaaa 
      ttcggttac gaccctcga aaggaccca aaagcgcga 
      atataccaa acactagac tacagaata cgaacagca 
      gaaagtaca aaattaaaa gttacggca aaaaggaat 
      tatgccgga aaaacgtcc gattttttg ggctctacc 
      aacgttaag aaaatatcc ctgaaacac gacatgtcg 
      gcaaagccc aaaatggaa ctagatgag agatactac 
      gttttaaga ccgttaaca atcccacct gcgagacaa 
      atgttctat caattgtgt gacttgatc ctaccgcaa 
      attcaagaa aaaatttcg aggttgccc aagtgttcg 
      ggtggttat tgcgattcc aaaaacggt tggttgcct 
      gataatttt atcagggat tgccgcaaa atcgtcaat 
      gatcacgtg ttggaagcc gtccagagg gagttactt 
      gaagataaa aagtttctc caggaaaaa acatcaaag 
      gaaaaggag cgcaaaaag gaaagtggg tcaaatctg 
      gactattac aaccaaatg ttgagcaat attaagaaa 
      gggatttac aaaaatgtg gggctccag ccggcttac 
      gtccccaaa gattttgat ccaggcccg tctagtgaa 
      ctgatcagt ttgtcaagt gacgaagaa aacgaacta 
      accgtcggc gaaataatc caaagttcg atccctgaa 
      aaagtggac gaaggcgaa gaggaggaa cagtccatc 
      agcgagtcg tcagacgaa gcggaaatt gacatggaa 
      gcggtgcag gaaattaac gaaatgatt tcgcaattc 
      aatgtaaaa atgtaa
    mRNA sequence
    • >TC010945 RA
      gataaaagt tttctcttt tatcaactg tgagacgtc 
      aaaaaaaca tcaaaacat aacctaaaa aatgtgcac 
      attttacaa gtaattgct tagttttct ttattcacg 
      tttatttac gcaagctaa atggatgct gaaacaata 
      aacgtttca aaaccattt gaaatagag tcacaactc 
      gacctgaaa aaggacgat aggaacacc aactctgca 
      gtcaagcac aagaaacag tcggaaaat gaggctttg 
      tatcaaaca cagtatgaa aatactctt gatcctgat 
      tgcattttg caaataaat aaacgctta attcgagtt 
      gagtatccc ggggaagtg aaaaatgtc aataaggcg 
      ttggagact ttgggagga atagaacac attgaaacg 
      agtgtggct gatcatgga cgtaaacta gaattacac 
      tttcacccc gaaaacaag tacaataaa ggctgtgtt 
      gcggataag gattttaaa ccaggaatt ttaatcaaa 
      gtgaaaaag aagttccta caacctgaa ccaccatca 
      gatgacatt agttacgat gtggaaggg gtttctgtg 
      ataaacttc agcttcaac aggctttgt gacttccag 
      tacttacct ctagtgtca aaagaacat gaaaccaag 
      tcgtctaaa acagagtac atttatgag aaaattgtt 
      ccacatgaa ttgccaact tctgaatgg cttgaaagc 
      gaagaggca caaaatatg gaacagttt ttacttcca 
      gttcagttt tcaccttac gacagttct caaaaaaaa 
      ctgaatgtc gaaagtgat aaaaacgac cctaaattg 
      aaactcaag ggccttact tgtgccgaa tctaaagtc 
      agttcaaag gagcagaaa aatgtttgc aggatattc 
      gggaaaaat aggcaacaa acgtcaatt tttgttact 
      tttcaagac aaggaaata ccgacgggg cccttagaa 
      agcgctttg aaacttctg aagcacagg aatttggaa 
      gaacgcatt gagagggtc aaacaattg tttgaggag 
      aggcccatc tggacgaag gccgccatt cgatacaac 
      acaggtctc tcagacgaa cattccaaa ataattctc 
      ccagtcgtt gcttattat tttagcaac ggcccttgg 
      cgaatttcc tgggtcaaa ttcggttac gaccctcga 
      aaggaccca aaagcgcga atataccaa acactagac 
      tacagaata cgaacagca gaaagtaca aaattaaaa 
      gttacggca aaaaggaat tatgccgga aaaacgtcc 
      gattttttg ggctctacc aacgttaag aaaatatcc 
      ctgaaacac gacatgtcg gcaaagccc aaaatggaa 
      ctagatgag agatactac gttttaaga ccgttaaca 
      atcccacct gcgagacaa atgttctat caattgtgt 
      gacttgatc ctaccgcaa attcaagaa aaaatttcg 
      aggttgccc aagtgttcg ggtggttat tgcgattcc 
      aaaaacggt tggttgcct gataatttt atcagggat 
      tgccgcaaa atcgtcaat gatcacgtg ttggaagcc 
      gtccagagg gagttactt gaagataaa aagtttctc 
      caggaaaaa acatcaaag gaaaaggag cgcaaaaag 
      gaaagtggg tcaaatctg gactattac aaccaaatg 
      ttgagcaat attaagaaa gggatttac aaaaatgtg 
      gggctccag ccggcttac gtccccaaa gattttgat 
      ccaggcccg tctagtgaa ctgatcagt ttgtcaagt 
      gacgaagaa aacgaacta accgtcggc gaaataatc 
      caaagttcg atccctgaa aaagtggac gaaggcgaa 
      gaggaggaa cagtccatc agcgagtcg tcagacgaa 
      gcggaaatt gacatggaa gcggtgcag gaaattaac 
      gaaatgatt tcgcaattc aatgtaaaa atgtaacac 
      aatttcgat tctttctgt atttcttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY