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Gene information
General information
TC013906 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 481446..486072
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013906
    iBeetle screen iB_09965

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013906 RA
      MEQSVCPLL KDPKAYNPD TIDLINDIK QRNYWLPCL 
      ENMVKKFVT KASFLNPDN PAATEKAEV CFQKFHELV 
      VKALEDPSI LAPLSIRTL LEFNEDNLR SSNFKDAWY 
      KQKETETNA ALREFKERL DFIDSIADF ESKWLEIVT 
      GVLAGNVFD WGSTVVADI LETSTNFGL SQAMSTIEK 
      RPWFKDNLD NWIERIKKE PYKEAVVFV DNAGVDFVL 
      GILPLIREF LKHNTKVIV TANSSPALN DVTWHELKQ 
      YSQQAASQC PILEKSLRT GQLVTVENG QKGPCLDLS 
      NLSPELCRL MSGADLVVL EGMGRAVHT NLYAKFNVD 
      SVKLAVLKN DWLAKSLGA QQFSVIFNY EAAT
    CDS sequence
    • >TC013906 RA
      atggaacag tccgtgtgc cccttactc aaagacccc 
      aaagcctac aatcctgac acgattgat ctaatcaat 
      gacatcaaa caacgcaat tactggttg ccgtgtctg 
      gaaaacatg gtcaagaaa ttcgtcacg aaggccagt 
      tttttgaac ccagacaat ccagctgcc accgaaaag 
      gccgaggtt tgtttccag aagttccac gagttggtc 
      gtcaaagcg ctggaagat cccagcatt ttagcgcct 
      ctgagcatc cgcaccttg ctcgaattt aacgaagat 
      aatttgagg agtagcaac tttaaagac gcttggtac 
      aaacagaaa gaaaccgaa acaaatgcg gctcttaga 
      gaattcaaa gagagatta gacttcatt gattcaatt 
      gctgatttc gagtcaaaa tggctggaa attgttact 
      ggcgttttg gcaggaaat gttttcgat tggggatca 
      actgttgta gctgatatt ttagaaact tcaaccaat 
      ttcgggctg tcacaagcc atgagtaca attgaaaag 
      agaccttgg tttaaagat aacttggac aattggatt 
      gaacgaatt aagaaagag ccttataag gaagctgtt 
      gttttcgtt gataacgct ggagtggac tttgtgcta 
      gggattttg cctcttatt cgagaattt ttgaaacac 
      aatactaaa gtcattgtg actgcaaat tcatctcca 
      gcactgaac gatgtcacg tggcatgaa ttaaaacaa 
      tactcgcaa caagcagcc agccagtgc cccatttta 
      gaaaagtcg ctaaggact ggacaactt gtaaccgtt 
      gaaaatgga caaaagggg ccctgttta gatctgtca 
      aacttatca cctgaacta tgtcgactt atgtcagga 
      gcggatttg gtcgttctg gaaggaatg ggacgagcg 
      gtgcataca aacctctac gcgaaattt aatgtggac 
      agtgtgaag ttagccgtt ttaaaaaat gattggttg 
      gcgaagagt ttaggagca caacaattt tcggttatt 
      ttcaattac gaggcagca acttaa
    mRNA sequence
    • >TC013906 RA
      tgtcatttt tgacattga catgattaa aacgtcaac 
      gtcagataa ttacaaatc caatattta aaaaaatgc 
      atcaatatt acaaaactt gcaaaatgc gaatgtgac 
      gcagtcgtg tggggagag caaatctcg ataaaattg 
      tttaaatta gaaatccag tctgtgatt atcagacac 
      gaggatgac ttgtttgtt gacaaaata aaagtgtga 
      caataactt tggacttaa ctcgaataa atcattttg 
      ataccacct tgaacataa agtgcttag acgaatcaa 
      agaagaaca atacaaatg aagtgaggt taggatgga 
      acagtccgt gtgcccctt actcaaaga ccccaaagc 
      ctacaatcc tgacacgat tgatctaat caatgacat 
      caaacaacg caattactg gttgccgtg tctggaaaa 
      catggtcaa gaaattcgt cacgaaggc cagtttttt 
      gaacccaga caatccagc tgccaccga aaaggccga 
      ggtttgttt ccagaagtt ccacgagtt ggtcgtcaa 
      agcgctgga agatcccag cattttagc gcctctgag 
      catccgcac cttgctcga atttaacga agataattt 
      gaggagtag caactttaa agacgcttg gtacaaaca 
      gaaagaaac cgaaacaaa tgcggctct tagagaatt 
      caaagagag attagactt cattgattc aattgctga 
      tttcgagtc aaaatggct ggaaattgt tactggcgt 
      tttggcagg aaatgtttt cgattgggg atcaactgt 
      tgtagctga tattttaga aacttcaac caatttcgg 
      gctgtcaca agccatgag tacaattga aaagagacc 
      ttggtttaa agataactt ggacaattg gattgaacg 
      aattaagaa agagcctta taaggaagc tgttgtttt 
      cgttgataa cgctggagt ggactttgt gctagggat 
      tttgcctct tattcgaga atttttgaa acacaatac 
      taaagtcat tgtgactgc aaattcatc tccagcact 
      gaacgatgt cacgtggca tgaattaaa acaatactc 
      gcaacaagc agccagcca gtgccccat tttagaaaa 
      gtcgctaag gactggaca acttgtaac cgttgaaaa 
      tggacaaaa ggggccctg tttagatct gtcaaactt 
      atcacctga actatgtcg acttatgtc aggagcgga 
      tttggtcgt tctggaagg aatgggacg agcggtgca 
      tacaaacct ctacgcgaa atttaatgt ggacagtgt 
      gaagttagc cgttttaaa aaatgattg gttggcgaa 
      gagtttagg agcacaaca attttcggt tattttcaa 
      ttacgaggc agcaactta aactcaagt gacctgtga 
      tcaattagt attaagaaa ctttttcac tgatagtga 
      tcaaaaggg ttgttacat ccaatagga aatctttat 
      gtatatacc tactgccac ccaaatgtc tagtgaaga 
      aaataaagt tgtgtcaat ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY