Status
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Gene information
General information
TC008523 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 12172889..12179225
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008523
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09964

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008523 RA
      MGARFSFDL KCVLLYISV LLLLVFLLK YVWNKRFLY 
      YYGSKISGP FALPLIGSA YYFTGGAKV LYDKLWQIC 
      EKYQPLAKI WLGPHLMVI PSHPKDVEY LLHNFVSKG 
      QLYEYITPL FGHGLLTGT VEKWKDHRK LINASFNSK 
      ILNSFIDIF VTHAKSSLD VFKEFCGKG QVDILPVFL 
      RINIDGICE TAMGVKPGT LSDRYDYLD WMMRAEHLI 
      MVRMFRPWL YPDFIWKNT NMGKEQKQL CKKSWKFIA 
      NIIKQKRGE KINTLHNDL KKLSFLEYF IDLTDEQQK 
      WCEKELIEE AQTMVFTGS GSLAITECY VLLMLAIHP 
      EIQEKIYEE LLTIFGNSD RDLCFKDLS KLCYLDRVI 
      KETLRLFPI APYIARLLD SDIHMEKYV IPKGTFALI 
      PITYLQRNS NLWSEPLRF DPDRFLPEQ VATRERCVY 
      LPFSYGSRN CIGLKYAEI SLKATLATI LRKYKVTSC 
      IYKSVEEIE FEFTMFIKP TRGSFVQIE ERIR
    CDS sequence
    • >TC008523 RA
      atgggtgct cgattttcc tttgattta aaatgtgta 
      ctattgtac atatctgtg cttttgttg ttagtcttt 
      cttttaaaa tacgtgtgg aataaacga tttttgtat 
      tactatggt tcaaaaatt agtggtcct tttgcctta 
      cctttgatc ggaagtgcg tactatttt acaggagga 
      gcaaaagtt ttgtacgat aaactctgg caaatatgt 
      gaaaaatat caaccctta gctaaaatt tggttaggc 
      cctcatttg atggtaatt ccatcacat ccaaaagat 
      gttgaatac ttgttacac aattttgta agtaaagga 
      caattatac gaatatata actccatta tttggacat 
      ggattatta acgggaact gttgaaaaa tggaaggat 
      cacagaaaa ctaatcaat gctagtttt aattcaaaa 
      attttaaat tcttttatc gatattttc gtcacacat 
      gcaaagagt tcgttagat gtgtttaag gagttttgt 
      gggaaagga caagtcgat atattacca gttttcttg 
      cgaattaat attgatgga atttgtgaa actgcaatg 
      ggagtaaaa cctggcact ttgagcgat agatatgat 
      tatttggat tggatgatg agggctgaa catctaata 
      atggttcga atgtttcga ccatggctt tatcccgat 
      ttcatttgg aaaaatact aatatgggc aaagaacaa 
      aaacaactt tgcaaaaag tcttggaaa tttattgca 
      aacatcatt aaacaaaag aggggcgaa aagataaac 
      acacttcat aacgattta aagaaatta tcttttctg 
      gaatatttc attgatttg accgacgaa cagcaaaaa 
      tggtgtgaa aaagagttg atagaagaa gcccaaaca 
      atggtattt acaggaagt ggctctttg gctataaca 
      gagtgttat gtactcctt atgttagcc atacatcca 
      gaaattcag gaaaaaata tacgaggag cttcttaca 
      atttttgga aactctgat cgagattta tgtttcaaa 
      gatttaagc aaactttgt tatcttgat cgtgtgata 
      aaagaaacg ttgagacta tttcccatt gcgccctac 
      attgctcgt cttcttgat tcagatata cacatggaa 
      aagtatgtg atacctaaa ggcacattt gctctaatt 
      ccaatcact tatctgcaa aggaattcg aatttatgg 
      tcggaacct ttaaggttt gatcctgat aggtttctt 
      cccgagcag gttgcgaca agggagcgg tgtgtttat 
      ttaccattc agttatggt tcaagaaat tgtataggt 
      ctaaaatat gctgaaatt tctttaaag gctactctt 
      gctaccata ctgagaaag tacaaagta acgtcttgt 
      atttataaa agtgtggaa gaaattgaa ttcgaattt 
      acaatgttt attaaaccc acaagaggt agttttgta 
      caaatagaa gaaagaata agatga
    mRNA sequence
    • >TC008523 RA
      tttgtgaaa aacagttta gctaataaa taatgggtg 
      ctcgatttt cctttgatt taaaatgtg tactattgt 
      acatatctg tgcttttgt tgttagtct ttcttttaa 
      aatacgtgt ggaataaac gatttttgt attactatg 
      gttcaaaaa ttagtggtc cttttgcct tacctttga 
      tcggaagtg cgtactatt ttacaggag gagcaaaag 
      ttttgtacg ataaactct ggcaaatat gtgaaaaat 
      atcaaccct tagctaaaa tttggttag gccctcatt 
      tgatggtaa ttccatcac atccaaaag atgttgaat 
      acttgttac acaattttg taagtaaag gacaattat 
      acgaatata taactccat tatttggac atggattat 
      taacgggaa ctgttgaaa aatggaagg atcacagaa 
      aactaatca atgctagtt ttaattcaa aaattttaa 
      attctttta tcgatattt tcgtcacac atgcaaaga 
      gttcgttag atgtgttta aggagtttt gtgggaaag 
      gacaagtcg atatattac cagttttct tgcgaatta 
      atattgatg gaatttgtg aaactgcaa tgggagtaa 
      aacctggca ctttgagcg atagatatg attatttgg 
      attggatga tgagggctg aacatctaa taatggttc 
      gaatgtttc gaccatggc tttatcccg atttcattt 
      ggaaaaata ctaatatgg gcaaagaac aaaaacaac 
      tttgcaaaa agtcttgga aatttattg caaacatca 
      ttaaacaaa agaggggcg aaaagataa acacacttc 
      ataacgatt taaagaaat tatcttttc tggaatatt 
      tcattgatt tgaccgacg aacagcaaa aatggtgtg 
      aaaaagagt tgatagaag aagcccaaa caatggtat 
      ttacaggaa gtggctctt tggctataa cagagtgtt 
      atgtactcc ttatgttag ccatacatc cagaaattc 
      aggaaaaaa tatacgagg agcttctta caatttttg 
      gaaactctg atcgagatt tatgtttca aagatttaa 
      gcaaacttt gttatcttg atcgtgtga taaaagaaa 
      cgttgagac tatttccca ttgcgccct acattgctc 
      gtcttcttg attcagata tacacatgg aaaagtatg 
      tgataccta aaggcacat ttgctctaa ttccaatca 
      cttatctgc aaaggaatt cgaatttat ggtcggaac 
      ctttaaggt ttgatcctg ataggtttc ttcccgagc 
      aggttgcga caagggagc ggtgtgttt atttaccat 
      tcagttatg gttcaagaa attgtatag gtctaaaat 
      atgctgaaa tttctttaa aggctactc ttgctacca 
      tactgagaa agtacaaag taacgtctt gtatttata 
      aaagtgtgg aagaaattg aattcgaat ttacaatgt 
      ttattaaac ccacaagag gtagttttg tacaaatag 
      aagaaagaa taagatgat taggttttt gttattatt 
      tttattaat gttgtagtt atagtaact aaataatag 
      tagtttatt atacaagta aagaaaagt ttttcaacg 
      aaacattgt tgaaagtac aacaatgtt tcgttcaaa 
      aactgacta aatggactg aaacaagtt gtcaagcta 
      atttagttt tttttatta agttttcga aaagatgtt 
      tgaaaaggc tttgatttt agtttaaaa acgtgctaa 
      aaaatcgaa aagacaaca attttttag tttaagaga 
      actgtcgga gaaaacttg aaattgtgg ttataaaga 
      ctttgatgg tgaattaat attttttta tttttaggc 
      ttcttttga tcgcactga atattattt taaaaacta 
      attaaattc aatcaactg tgtcttcta cactcaaat 
      aaatttatt cgctctaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY