Status
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Gene information
General information
TC004658 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1028687..1031504
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004658
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09962

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004658 RA
      MPKKFSTEN TKAVAARER KKAAKDAEV 
      AKKQQQLEE AYWQDDDKL IKKKQQRKE 
      EQERKREQQ LAKKQEAKQ LLEQEMSSI 
      KKAGKPAPP PKVTRSQIS DTIAKKEPE 
      KKEKVVTHL DEPLQENIN RLKIEGEEA 
      RTVDEAIGI LSSKPDDID KHPERRMKS 
      AFTAYETRR LEELKAENP TLRLSQLKQ 
      MIFKEWQKS PENPLNKQ
    CDS sequence
    • >TC004658 RA
      atgccaaaa aaattctcc accgaaaat accaaagct 
      gtggcagca cgcgaacgt aaaaaagct gcaaaagac 
      gccgaagtg gccaaaaaa cagcaacaa ttagaagaa 
      gcctactgg caagatgac gacaaatta atcaaaaag 
      aagcaacag agaaaggag gagcaagaa cgtaaaagg 
      gagcaacaa ttagccaaa aaacaggaa gccaagcag 
      ctcctggaa caggaaatg agctctatt aaaaaagcc 
      ggaaagcct gcgccgcca cccaaagtg acacgatcc 
      caaatttcg gacaccata gccaaaaaa gagccagaa 
      aagaaggaa aaagttgta acgcatctg gacgaaccc 
      ttgcaggaa aacatcaac aggctcaag attgagggg 
      gaggaggcc aggactgtg gacgaggcc attggaata 
      ttgagctcg aaaccggat gatatcgac aagcacccc 
      gagcgtcgg atgaagtct gcatttaca gcgtacgag 
      acgagacgt ctggaggaa ctgaaggct gaaaacccc 
      acacttcgc ctgtcccag ttgaagcaa atgatattc 
      aaagagtgg caaaaatcg ccggagaat cctctgaat 
      aaacaataa
    mRNA sequence
    • >TC004658 RA
      ggttgaggt tacatattt atttacaaa agtattact 
      ttacattta cattgaatg acaaaaaaa tgccaaaaa 
      aattctcca ccgaaaata ccaaagctg tggcagcac 
      gcgaacgta aaaaagctg caaaagacg ccgaagtgg 
      ccaaaaaac agcaacaat tagaagaag cctactggc 
      aagatgacg acaaattaa tcaaaaaga agcaacaga 
      gaaaggagg agcaagaac gtaaaaggg agcaacaat 
      tagccaaaa aacaggaag ccaagcagc tcctggaac 
      aggaaatga gctctatta aaaaagccg gaaagcctg 
      cgccgccac ccaaagtga cacgatccc aaatttcgg 
      acaccatag ccaaaaaag agccagaaa agaaggaaa 
      aagttgtaa cgcatctgg acgaaccct tgcaggaaa 
      acatcaaca ggctcaaga ttgaggggg aggaggcca 
      ggactgtgg acgaggcca ttggaatat tgagctcga 
      aaccggatg atatcgaca agcaccccg agcgtcgga 
      tgaagtctg catttacag cgtacgaga cgagacgtc 
      tggaggaac tgaaggctg aaaacccca cacttcgcc 
      tgtcccagt tgaagcaaa tgatattca aagagtggc 
      aaaaatcgc cggagaatc ctctgaata aacaataat 
      gtcgcaacc aagtgatca tcatcgtga atatgccga 
      aataatttc ttacgcact aacctactt atacttgac 
      gctttattg ttttaccgg ttttattag acgaattgt 
      tatgaagtc agtgtattt tgttggtac cgttaatag 
      ataatttat gacacacaa atatttcat ttcattgca 
      taaccctat aaatcatgt cgcgtttcg tgtgggtaa 
      ggctggagg agggacgtc gggaatgtt tgggtttct 
      cttcaggtt tgcgatctc gtgtttagg atcatcggc 
      tttttggta aatttcttc gggtagtgt ttttggctt 
      ttcgggctg agtttcctg cgcgtagta gcccggagg 
      ggcgtaggc gtatggtac gaaccgctc cacaggtag 
      tggttgtag cgcactttg taactcctc attttctgg 
      tttgcctac tttcggtgg tgtcgcaac cttaaaata 
      acaactcgt gctgttctc ctggcagag tctcactta 
      cgaaggtgg cgatcggcg attgataga aaaagggcg 
      gtagtgcta ttccgaggg atgttgtcc tcaacaccg 
      gctcgtaga cggggaggg catgactag gggataaat 
      tcttcttcg tgcttatca aaacgttgt atatctttg 
      gggttctcg gatcagcac gtaccagga ataacagtg 
      ctgacaatg aaaacaatc tgtacatag tatctgcaa 
      aaagacatt gaattaaag gtcaccgcc ttcatcaag 
      cagatctcc gaatttttt aggtaaacc tgtttgagt 
      aatgatgca gtgagacta atctctagt tacggttgg 
      ctacgctct tcagtaacg tggattcaa tgattttcg 
      caggaactg agtaatgct gaatttaat ttttgccaa 
      accgcacgt cccgattat aattgcaaa tcggagact 
      gtaattgat ccagttttt cggctgcgt gttaattac 
      accaagggt gattaattg ttaatttac acaggccac 
      tgaatggac gatttatga aaaggtcgc cggtggact 
      tagcgcact tggaaaaat tgaatgaac actagcaca 
      tgttattgt ttaccttgt ataaattgt ttcacgtca 
      caactgcaa ataattgcc ttgatagaa cagagaggt 
      cttatatag ttcttgagg ttgttcttt gtgccgctc 
      aactattcg tgttcactt gtgaagtac atttttcag 
      ataaatccc ttcactggg agccttgca tacgaccaa 
      ggtatttgc aattaatta cgttgttgt gatcactcc 
      gagtaaatt ttcccacgg atgaatgaa aattttttt 
      gatggacct gacttaggc gtgggcctg atgaaatat 
      tccaaggaa aaaggcatt agccaactg gaaggagtg 
      tcggaaatc atccgagcc aggtgcaca ttgtctcag 
      taccaagcc tcttaccaa ttca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY