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Gene information
General information
TC008405 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9849498..9851845
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008405
    iBeetle screen iB_09959

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008405 RA
      MLEPIDISP NQDKGVLKE ILREGTSDE CPPPGSKVR 
      VHYTGTLTD GTKFDSSRD RNEPFEFDL GKGSVIKAW 
      DIGVATMKK GEQAMLTCA PEYAYGKSG SPPTIPPDA 
      TLKFDVEVI SWKCEDLSP KKDGGIERA QIKAGEGYT 
      YPNDGATVE VHLIGKYEN KEFDVRDVT FTVGEASEQ 
      NVISGIDIA IEKFKKGET SKLIIKPQY AFGTEGCSE 
      FNIPPNATV EYTVTLKNF ERVKESWAL DTKERVEQS 
      KIFKEKGTN YFKANKFQL AIKMYKKII EYLESQKDP 
      ELDKEIESL NLAAHLNLS LCYLKIDDN FEAKASATA 
      ALKMDPDNV KALFRRGQA LLKLGEPKL ASEDFQQCL 
      KLDPGNTAA QSQKALCVK TLKEQLEKE KKVFANMFD 
      RFAKVDAQR EEKEKKKEP NVMSSVGEW GKEDREREP 
      SEFEKENPN ILLLSGSGE FKDM
    CDS sequence
    • >TC008405 RA
      atgcttgaa cctattgat atttcccca aatcaagac 
      aagggagtc ctcaaggaa atccttcgg gagggtaca 
      agtgacgag tgccctcca cctggatcc aaagtcaga 
      gttcactac actggaact ctaactgat ggcacaaaa 
      ttcgactct agtagagac cggaacgag cctttcgag 
      tttgatttg ggcaaaggg agtgtgatt aaagcatgg 
      gacatcggt gtggccact atgaaaaag ggggagcaa 
      gctatgctc acatgtgca cccgaatac gcctacggc 
      aagtctggc agtcctcca actattcca ccagacgcc 
      acgctgaaa tttgatgtt gaagtaatc agttggaag 
      tgcgaggat ttgagccct aagaaagac gggggtatt 
      gaaagggcc caaattaag gcgggtgaa gggtacacg 
      tatcccaac gatggggct accgttgaa gtgcatttg 
      attggtaaa tacgaaaac aaggaattt gatgttcgt 
      gatgtcacg tttacggtt ggggaagcc tcagaacag 
      aatgtcatt tccggtatt gacattgcc attgaaaaa 
      ttcaaaaag ggggaaact tcaaaactt ataatcaag 
      ccacaatat gcatttgga actgagggt tgttcagaa 
      ttcaacatt ccaccgaac gcaacggtt gaatacacc 
      gtcacgttg aagaacttt gaaagagtt aaagaaagt 
      tgggctctt gacaccaaa gaacgtgtg gaacaatca 
      aaaatcttc aaggagaaa ggaacgaat tattttaaa 
      gctaacaaa tttcaacta gccataaaa atgtacaaa 
      aaaattatt gagtatttg gaatctcag aaagatccg 
      gaactggat aaagaaata gagtcgtta aatttagct 
      gcgcatctg aatttgtcg ttatgttac ttgaaaatt 
      gacgacaat ttcgaagct aaagcctcg gctaccgcc 
      gctttgaaa atggatcca gacaatgtt aaagctttg 
      tttagaaga ggtcaggct ttactaaaa ctaggagag 
      cccaagctt gcttccgaa gatttccag cagtgtttg 
      aagctggat ccaggaaat acagctgca cagagtcag 
      aaagccctt tgtgtgaaa acactcaaa gaacagtta 
      gagaaggag aagaaggtt tttgctaat atgtttgac 
      agatttgcg aaagtggat gctcagagg gaagaaaaa 
      gagaaaaag aaagagccg aatgtgatg tcgtcagtg 
      ggtgaatgg ggtaaggaa gatcgtgag cgagagccg 
      agtgagttt gagaaggaa aatccgaat attttgttg 
      cttagtggc agtggagaa tttaaggac atgtag
    mRNA sequence
    • >TC008405 RA
      cagttcgta ccattaatt tgaaaggtg tgtttgtgc 
      tttttccga ttaattttt gaataaaaa cccaacttg 
      caaaaaagt acattttgt ttaaaagcc ggctctaga 
      atattccac cataaccat gcttgaacc tattgatat 
      ttccccaaa tcaagacaa gggagtcct caaggaaat 
      ccttcggga gggtacaag tgacgagtg ccctccacc 
      tggatccaa agtcagagt tcactacac tggaactct 
      aactgatgg cacaaaatt cgactctag tagagaccg 
      gaacgagcc tttcgagtt tgatttggg caaagggag 
      tgtgattaa agcatggga catcggtgt ggccactat 
      gaaaaaggg ggagcaagc tatgctcac atgtgcacc 
      cgaatacgc ctacggcaa gtctggcag tcctccaac 
      tattccacc agacgccac gctgaaatt tgatgttga 
      agtaatcag ttggaagtg cgaggattt gagccctaa 
      gaaagacgg gggtattga aagggccca aattaaggc 
      gggtgaagg gtacacgta tcccaacga tggggctac 
      cgttgaagt gcatttgat tggtaaata cgaaaacaa 
      ggaatttga tgttcgtga tgtcacgtt tacggttgg 
      ggaagcctc agaacagaa tgtcatttc cggtattga 
      cattgccat tgaaaaatt caaaaaggg ggaaacttc 
      aaaacttat aatcaagcc acaatatgc atttggaac 
      tgagggttg ttcagaatt caacattcc accgaacgc 
      aacggttga atacaccgt cacgttgaa gaactttga 
      aagagttaa agaaagttg ggctcttga caccaaaga 
      acgtgtgga acaatcaaa aatcttcaa ggagaaagg 
      aacgaatta ttttaaagc taacaaatt tcaactagc 
      cataaaaat gtacaaaaa aattattga gtatttgga 
      atctcagaa agatccgga actggataa agaaataga 
      gtcgttaaa tttagctgc gcatctgaa tttgtcgtt 
      atgttactt gaaaattga cgacaattt cgaagctaa 
      agcctcggc taccgccgc tttgaaaat ggatccaga 
      caatgttaa agctttgtt tagaagagg tcaggcttt 
      actaaaact aggagagcc caagcttgc ttccgaaga 
      tttccagca gtgtttgaa gctggatcc aggaaatac 
      agctgcaca gagtcagaa agccctttg tgtgaaaac 
      actcaaaga acagttaga gaaggagaa gaaggtttt 
      tgctaatat gtttgacag atttgcgaa agtggatgc 
      tcagaggga agaaaaaga gaaaaagaa agagccgaa 
      tgtgatgtc gtcagtggg tgaatgggg taaggaaga 
      tcgtgagcg agagccgag tgagtttga gaaggaaaa 
      tccgaatat tttgttgct tagtggcag tggagaatt 
      taaggacat gtagttttg ttgcccaat tgaataaaa 
      tcgtttcaa ttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY