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Gene information
General information
TC008408 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9866318..9877038
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008408
    iBeetle screen iB_09958

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber mostlynot present - Number of animals showing the phenotype: 4

    egg chamber potentiallydegenerated - Number of animals showing the phenotype: 4

    tropharium structure irregular - Number of animals showing the phenotype: 4

    vitellarium potentiallysize decreased - Number of animals showing the phenotype: 3

    ovariole potentiallysize decreased - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008408 RA
      MINSNFVEG RKSIGGLGG FGACSIKVK NKVNQKCFF 
      NCWRFLRGV WSVSSQTTS YGQQRRPYC LSDFTWCEA 
      RVFEARPIT TTTESPRQT GPQARNSGK DAVPPVKRR 
      WVPPSTLRR QDALTPESR NDLVFRKVR GILNKLTPE 
      KFQKLSDDL LRIELSSSV ILKGVILLI FDKALDEPK 
      YSSMYAQLC KRLSEEAPN FESDGSTCT FRVLLLNKC 
      KLEFDNRAA ALESISSGL NDEDEERRQ VAKRKMLGN 
      IKFIGELGK LEILSEGIL HRCIQELLV RRGDDPSED 
      LECLCQIMR TCGRILDTD KGQKLMEQY FDRMKLLAA 
      NTDLQPRIR FMLKDTIDL RHNGWVPRK ATVVEGPMP 
      INQIRPADD DRPGFRRDR NNHDRDSDR SNLSELFRH 
      PMKTRSGID DMIMSMNLT TSSPNLIPT NTFGTNGFG 
      NQRDGGFRG HNNQRSGGY NNYNTNQRG QYKHNQNNA 
      NSQYNNQSN KEIAPRFKK NFIMPKEEI EDVELRPTT 
      MLFNKASVK TNNMMNSRS IEPSYTQSM KQPPATLHK 
      EPLPIKQVS SEKPKQSKK DKGPTKDEV LKRFGSLLD 
      EYWKGDIDL KQAVNLYKE HKVPDKFAK DVMLHGLSI 
      AVDKSDVEQ EKLIKLLSN LKEENAVNT NTLQDTFKS 
      LCNILDERE NEIEKTTNA IAYLFSVAI SEHLTLLSE 
      VASLTDNGA HYPLFLVVL QHLHQKRGK PELTELFNK 
      SKVNLLTQL PESDKTKER LSEILEERD LTFLYPLLR 
      IQAELARQL NVDPNPQSY YKWIKENLD PANFNDPGF 
      INALMTVLL KYITQEAAA SGDDKAVAE KEKSMLERY 
      QPILHAFLR EKLHLQLVA VYSLQVHLY SLNFPKGQL 
      LRWFMALYD LEIVDEEAF LNWKEDVTD AYPGKGQAL 
      FQVNQWLTW LQEAESEEE EGED
    CDS sequence
    • >TC008408 RA
      atgattaat tcgaatttc gtggaagga aggaaatcg 
      attgggggt ctagggggt ttcggcgcg tgtagcatt 
      aaagtaaag aataaagtg aaccaaaag tgtttcttt 
      aattgttgg cgattttta cgtggtgtg tggtcagtg 
      tcctcacaa acaacaagc tacggccag cagcggagg 
      ccgtattgc cttagtgat ttcacctgg tgcgaagcg 
      agagttttc gaggcgcgc ccgatcacc accacgacg 
      gagagcccc aggcagacg gggccacaa gctcgtaat 
      tcggggaag gacgctgtt ccccccgtg aagcgacgc 
      tgggtccct ccatcgaca ttgcgacgt caagatgcg 
      ctcaccccc gagtcccgt aacgacctc gtcttccgc 
      aaagtgcgc ggcatcctc aacaagctc actccggag 
      aagttccag aagctgagt gatgacctc ctccgcatc 
      gagctctcc tcgagcgtc atcctaaag ggcgtcatt 
      ctgctcatc tttgacaag gccctcgac gagccgaag 
      tactcgtct atgtacgcg cagctgtgc aagcgcctg 
      tcagaggaa gctccgaat ttcgagtcc gacggtagc 
      acgtgcacc ttccgcgtc cttctgctc aacaagtgc 
      aagttagag ttcgacaac cgcgcggcg gctttggaa 
      tcaatcagt tccggctta aacgacgaa gatgaggag 
      cgtcgtcag gtggccaag cgcaaaatg cttggcaat 
      atcaaattc atcggcgaa ctcggcaaa ttggaaata 
      ctgtccgag ggtatcctc caccggtgc atccaggag 
      ttactggtg cgccgcggc gacgatcct tccgaagac 
      ttggagtgt ttgtgtcaa atcatgcgc acatgcggg 
      cgcatcttg gacacggac aagggacag aaactcatg 
      gagcagtac ttcgatcgt atgaaacta ctagcggca 
      aacacggat ttgcagccc aggattcgt ttcatgttg 
      aaagacacg atcgacttg agacacaat gggtgggtg 
      cccagaaag gcaacggtt gtggaaggc cccatgccc 
      atcaatcag atcagaccg gccgacgac gatcgaccg 
      ggattcaga agggatcga aacaatcat gatagagac 
      tctgataga tcgaacctc tcggaattg ttccgacat 
      ccgatgaag accagaagt ggcatcgac gatatgatc 
      atgagcatg aatttaacc acttcatcg ccgaactta 
      ataccaacc aacacgttc ggcaccaac ggattcgga 
      aatcaaaga gacggagga ttccgagga cacaacaac 
      caacgcagc ggcggttac aacaactac aacaccaac 
      caacgtgga cagtataag cacaaccag aacaatgcc 
      aattcgcaa tataacaac caaagcaac aaagaaatc 
      gccccacgt ttcaagaag aacttcatt atgccaaaa 
      gaagaaatc gaagacgtg gaactcaga cctacgacc 
      atgcttttc aataaagcg tctgttaaa acaaacaac 
      atgatgaat agcagaagt atagagccg tcgtatact 
      cagagtatg aaacaaccg ccggctact ctacacaaa 
      gaaccattg cccatcaaa caagtaagc tctgagaag 
      ccaaaacag tcaaagaag gataagggt ccaacaaag 
      gatgaagtt ttgaagagg tttggctcg cttttggac 
      gagtattgg aaaggtgac atcgacctc aaacaagcc 
      gtcaatttg tataaagaa cataaagtc cccgataag 
      ttcgccaag gatgtaatg ttacacggt ttgtcgatc 
      gctgtagat aagtcggat gtggaacaa gagaagctg 
      atcaaatta ctgagtaat ttgaaagaa gagaacgcc 
      gtcaatact aacaccctt caagacacc ttcaagtca 
      ttatgtaac attctagac gaacgagaa aacgaaatc 
      gagaaaacc acaaacgct atagcttat ctcttctca 
      gttgcgatt tctgaacat ctgactttg ctatcggag 
      gtggcatcg ttgactgat aatggtgct cattatccg 
      cttttcctc gtcgtattg caacattta caccagaaa 
      cgtggcaaa cccgaactc accgaattg tttaataag 
      agcaaagtt aacctcttg actcagttg cccgaaagc 
      gacaagact aaagaacgt ctttcggag attttggaa 
      gagcgcgat ttgacattt ttatatcca ttgttgcgc 
      atccaagct gaattagcc agacagcta aacgtcgat 
      ccgaatcca cagtcgtac tacaaatgg atcaaagag 
      aatctagat ccggccaat ttcaacgat ccgggtttt 
      attaacgca cttatgacc gttcttttg aagtacata 
      acgcaagaa gcggccgca agtggtgat gataaagcc 
      gttgcagag aaggagaag agtatgctg gagcgttac 
      cagccgatt ttgcacgcg tttctaaga gagaagctt 
      catcttcaa ctcgttgca gtgtactct ttgcaggtg 
      cacttgtat tcgttgaac ttcccgaaa gggcaactg 
      cttaggtgg ttcatggcc ctctacgat ttggagatt 
      gtcgatgag gaggccttc ttgaactgg aaagaggac 
      gtcactgac gcctaccct ggcaaagga caagccttg 
      ttccaggtg aatcagtgg ctcacgtgg ctgcaggag 
      gccgaatct gaggaggaa gagggcgag gattag
    mRNA sequence
    • >TC008408 RA
      agagaaaaa gcaacaaaa tatttgatg tttacaagc 
      tatttttag cgataatga agtagccgg aattttgaa 
      tgattaatt cgaatttcg tggaaggaa ggaaatcga 
      ttgggggtc tagggggtt tcggcgcgt gtagcatta 
      aagtaaaga ataaagtga accaaaagt gtttcttta 
      attgttggc gatttttac gtggtgtgt ggtcagtgt 
      cctcacaaa caacaagct acggccagc agcggaggc 
      cgtattgcc ttagtgatt tcacctggt gcgaagcga 
      gagttttcg aggcgcgcc cgatcacca ccacgacgg 
      agagcccca ggcagacgg ggccacaag ctcgtaatt 
      cggggaagg acgctgttc cccccgtga agcgacgct 
      gggtccctc catcgacat tgcgacgtc aagatgcgc 
      tcacccccg agtcccgta acgacctcg tcttccgca 
      aagtgcgcg gcatcctca acaagctca ctccggaga 
      agttccaga agctgagtg atgacctcc tccgcatcg 
      agctctcct cgagcgtca tcctaaagg gcgtcattc 
      tgctcatct ttgacaagg ccctcgacg agccgaagt 
      actcgtcta tgtacgcgc agctgtgca agcgcctgt 
      cagaggaag ctccgaatt tcgagtccg acggtagca 
      cgtgcacct tccgcgtcc ttctgctca acaagtgca 
      agttagagt tcgacaacc gcgcggcgg ctttggaat 
      caatcagtt ccggcttaa acgacgaag atgaggagc 
      gtcgtcagg tggccaagc gcaaaatgc ttggcaata 
      tcaaattca tcggcgaac tcggcaaat tggaaatac 
      tgtccgagg gtatcctcc accggtgca tccaggagt 
      tactggtgc gccgcggcg acgatcctt ccgaagact 
      tggagtgtt tgtgtcaaa tcatgcgca catgcgggc 
      gcatcttgg acacggaca agggacaga aactcatgg 
      agcagtact tcgatcgta tgaaactac tagcggcaa 
      acacggatt tgcagccca ggattcgtt tcatgttga 
      aagacacga tcgacttga gacacaatg ggtgggtgc 
      ccagaaagg caacggttg tggaaggcc ccatgccca 
      tcaatcaga tcagaccgg ccgacgacg atcgaccgg 
      gattcagaa gggatcgaa acaatcatg atagagact 
      ctgatagat cgaacctct cggaattgt tccgacatc 
      cgatgaaga ccagaagtg gcatcgacg atatgatca 
      tgagcatga atttaacca cttcatcgc cgaacttaa 
      taccaacca acacgttcg gcaccaacg gattcggaa 
      atcaaagag acggaggat tccgaggac acaacaacc 
      aacgcagcg gcggttaca acaactaca acaccaacc 
      aacgtggac agtataagc acaaccaga acaatgcca 
      attcgcaat ataacaacc aaagcaaca aagaaatcg 
      ccccacgtt tcaagaaga acttcatta tgccaaaag 
      aagaaatcg aagacgtgg aactcagac ctacgacca 
      tgcttttca ataaagcgt ctgttaaaa caaacaaca 
      tgatgaata gcagaagta tagagccgt cgtatactc 
      agagtatga aacaaccgc cggctactc tacacaaag 
      aaccattgc ccatcaaac aagtaagct ctgagaagc 
      caaaacagt caaagaagg ataagggtc caacaaagg 
      atgaagttt tgaagaggt ttggctcgc ttttggacg 
      agtattgga aaggtgaca tcgacctca aacaagccg 
      tcaatttgt ataaagaac ataaagtcc ccgataagt 
      tcgccaagg atgtaatgt tacacggtt tgtcgatcg 
      ctgtagata agtcggatg tggaacaag agaagctga 
      tcaaattac tgagtaatt tgaaagaag agaacgccg 
      tcaatacta acacccttc aagacacct tcaagtcat 
      tatgtaaca ttctagacg aacgagaaa acgaaatcg 
      agaaaacca caaacgcta tagcttatc tcttctcag 
      ttgcgattt ctgaacatc tgactttgc tatcggagg 
      tggcatcgt tgactgata atggtgctc attatccgc 
      ttttcctcg tcgtattgc aacatttac accagaaac 
      gtggcaaac ccgaactca ccgaattgt ttaataaga 
      gcaaagtta acctcttga ctcagttgc ccgaaagcg 
      acaagacta aagaacgtc tttcggaga ttttggaag 
      agcgcgatt tgacatttt tatatccat tgttgcgca 
      tccaagctg aattagcca gacagctaa acgtcgatc 
      cgaatccac agtcgtact acaaatgga tcaaagaga 
      atctagatc cggccaatt tcaacgatc cgggtttta 
      ttaacgcac ttatgaccg ttcttttga agtacataa 
      cgcaagaag cggccgcaa gtggtgatg ataaagccg 
      ttgcagaga aggagaaga gtatgctgg agcgttacc 
      agccgattt tgcacgcgt ttctaagag agaagcttc 
      atcttcaac tcgttgcag tgtactctt tgcaggtgc 
      acttgtatt cgttgaact tcccgaaag ggcaactgc 
      ttaggtggt tcatggccc tctacgatt tggagattg 
      tcgatgagg aggccttct tgaactgga aagaggacg 
      tcactgacg cctaccctg gcaaaggac aagccttgt 
      tccaggtga atcagtggc tcacgtggc tgcaggagg 
      ccgaatctg aggaggaag agggcgagg attagacgc 
      ccgtcgttt aggaaaagt taaatctaa tttaagtta 
      ttaaataca cacagggac tctagtcct gtatttact 
      ttgtttttg gtgtcgaca atggttttg tagtcggta 
      aaatgtcgt taagcctcg atgaaaata atgtctttt 
      tgttttttt cgcgccgaa caacttcaa gttggaatt 
      gtttgtttg tgtgcgtga tgtttttta taacataat 
      tttaattga ttaattgaa taataggaa aagtgattt 
      agatctgta agtttatat acctattgc atgattgcc 
      acctaaatt gaatgtgac gtggttctg tacccgcac 
      ttaccactc ttgactgtg ttgataaat taatatttc 
      atgagcttt tcaaactat aatatatac ttttataac 
      aatgtgttc tctgtttat gtaattgaa tgtcctatt 
      atttttttc tcaagtatt ttgttatgg agaaatagt 
      taaattccc aatacactt agattagtc aatgtttca 
      cataaatta taaaatcat gtccttgtg aatatgatt 
      gtgttaata aactattcg gtggtattt tgctatcga 
      cgtaaattt taaatctct atttcttcc ccactcctt 
      cacatcact tacaataaa aataagtgc agttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY