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Gene information
General information
TC008418 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 10032081..10033681
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008418
    iBeetle screen iB_09957

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008418 RA
      MQNHTVILK FSLTLLIIQ LIKIASSNE TNSTLRLVH 
      VLFRHGDRT TDARTLYPK DPFINETYY PYGLGELTN 
      KGKQKAYKV GKALRKRYG AFLGEIYSP DILEAQSSD 
      TNRTKTSLQ LVLTGLFPP VGDQIWESG LNWQPIPFN 
      VLPRRQDSI FFGLTCSTF KQKFTEIVT APKWQKEFK 
      KHKTTFDYI AENTGLEVN NYFDVFHLY LCLTTEKEF 
      GFTLPEWTK NVYPQPLKD FAIKTYELM SATSELRRL 
      SSGGMLKKI VDDSKAKIS GELVPKNRK IFLYSGHEV 
      NLANMLHTL DVFEPQIPP YSSYILFEL HVINRVPGF 
      KIYYEDYTS TQPQPIKLP ACDEFCPLD QFIDLLSEY 
      FPRQDACNG NHK
    CDS sequence
    • >TC008418 RA
      atgcagaat cacacggta attctaaaa ttttcgctg 
      acactttta attatacaa ttaataaaa attgcgtct 
      tcaaacgaa accaattca accttgcga ctagtccat 
      gttcttttt cgccatggc gacagaact accgatgcc 
      agaactttg tatccgaag gaccccttc atcaacgaa 
      acgtattat ccttacggc ctcggagaa ttaacaaat 
      aaaggtaaa caaaaagct tacaaagtg gggaaagct 
      ctacgcaaa agatatggc gcttttctg ggcgaaatt 
      tactccccg gacatcctg gaagctcaa agttccgac 
      acaaatcga actaaaaca tcacttcag ctggtttta 
      accggattg tttcctcct gttggcgac caaatctgg 
      gaatctggt ttaaactgg caacccata ccgttcaat 
      gtcttacct cgaagacaa gattctatt ttcttcggt 
      ttgacttgt tccactttc aagcaaaaa ttcaccgag 
      attgtcacc gcacccaaa tggcaaaag gagttcaaa 
      aaacacaaa acaacgttt gactacatt gcggaaaat 
      accgggctt gaggttaat aattatttc gacgttttt 
      catttatat ctgtgtcta acgactgag aaagagttc 
      ggcttcact ctcccggaa tggacgaaa aacgtctat 
      cctcagcca ctcaaagat tttgccatc aaaacgtac 
      gaactcatg agtgccaca agtgaatta agaagactc 
      tcttcggga ggtatgttg aaaaaaatc gtcgacgat 
      tcaaaggcc aaaatttcg ggggagttg gtgcccaaa 
      aatcgcaaa atttttctc tactcggga catgaggtc 
      aatctcgcc aatatgttg cacactttg gacgttttt 
      gagccccaa ataccaccc tacagttct tacattttg 
      tttgagctt catgttatt aaccgcgtt cctggattc 
      aagatttat tacgaagat tacacttct acgcaaccc 
      caaccgatc aaacttccg gcctgtgat gaattttgc 
      cctttggac cagtttatt gacctactg tccgagtat 
      tttccccgt caggacgcg tgtaatggc aatcacaaa 
      taa
    mRNA sequence
    • >TC008418 RA
      tttaagtac gaccatgca gaatcacac ggtaattct 
      aaaattttc gctgacact tttaattat acaattaat 
      aaaaattgc gtcttcaaa cgaaaccaa ttcaacctt 
      gcgactagt ccatgttct ttttcgcca tggcgacag 
      aactaccga tgccagaac tttgtatcc gaaggaccc 
      cttcatcaa cgaaacgta ttatcctta cggcctcgg 
      agaattaac aaataaagg taaacaaaa agcttacaa 
      agtggggaa agctctacg caaaagata tggcgcttt 
      tctgggcga aatttactc cccggacat cctggaagc 
      tcaaagttc cgacacaaa tcgaactaa aacatcact 
      tcagctggt tttaaccgg attgtttcc tcctgttgg 
      cgaccaaat ctgggaatc tggtttaaa ctggcaacc 
      cataccgtt caatgtctt acctcgaag acaagattc 
      tattttctt cggtttgac ttgttccac tttcaagca 
      aaaattcac cgagattgt caccgcacc caaatggca 
      aaaggagtt caaaaaaca caaaacaac gtttgacta 
      cattgcgga aaataccgg gcttgaggt taataatta 
      tttcgacgt ttttcattt atatctgtg tctaacgac 
      tgagaaaga gttcggctt cactctccc ggaatggac 
      gaaaaacgt ctatcctca gccactcaa agattttgc 
      catcaaaac gtacgaact catgagtgc cacaagtga 
      attaagaag actctcttc gggaggtat gttgaaaaa 
      aatcgtcga cgattcaaa ggccaaaat ttcggggga 
      gttggtgcc caaaaatcg caaaatttt tctctactc 
      gggacatga ggtcaatct cgccaatat gttgcacac 
      tttggacgt ttttgagcc ccaaatacc accctacag 
      ttcttacat tttgtttga gcttcatgt tattaaccg 
      cgttcctgg attcaagat ttattacga agattacac 
      ttctacgca accccaacc gatcaaact tccggcctg 
      tgatgaatt ttgcccttt ggaccagtt tattgacct 
      actgtccga gtattttcc ccgtcagga cgcgtgtaa 
      tggcaatca caaataatt caggatgtt aatttggtg 
      ctttcctcg tctggagcc cacatcagg aaagacaac 
      aagtcaaat cccaagtaa acaaccatc aaaatcaga 
      acgtgtctc tcggttaac gtcggcaaa ctttgaaaa 
      agtgtgtcc atgtttgtg taaattctg gaaatagtt 
      tgcatattt tctacaaag aattttgtt gtaatttta 
      aatccccaa taaataatc ttgacactg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY