Status
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Gene information
General information
TC011513 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3822038..3826828
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011513
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09947

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011513 RA
      MSNTSFILY IPEVALEKI FSFLTYDEI AKNRLDLLD 
      SLLSVATEE LKRDNAPVR TLTEADLFG KDEDEKPKN 
      PSKSSIHTG DTDSSDDED NKYHEERKY NDYGREIKH 
      LLESNHSAP NSPISQPEV SWKNQTVKP KILPKIQDA 
      QSDPVFGLR IVNPTISFN TLQDRMVGR EAVAFSRVK 
      KFVSGNISD KNWVIAGVI AGKSSVKTS QKGNQFIIW 
      CLSDLKNDI KTVSVLLFG SAYKQLWKT ATGTVVGIL 
      NPTVLETRD NSRDEATLS VDNSQKVMI MGQSKDLGT 
      CKSTKKSGA QCTNIVNTS HCEYCIYHI KQEYQKCSK 
      RAELQSSFV GKGLTALRN KVLGKNEVF YAGKSYTAI 
      PAKRSKKLE QKEKNLLQA LSGNAPLTR KPTSTKKTS 
      KVAANIEVT QRQRLRDLS LLDRLGGYG VKTDFKGTH 
      SAEVTLESS KKTAIEVIS KLKAVAKTT DKVEPVAKL 
      QEVTATHSA EVTLESSKK TALEAISKL KSATNSKKM 
      EPVLRPQEV ISENTKKTS IEIISKVDV PVLLGSDKG 
      TIDLGASVT PQQINRAKL NALKYVQKN GPLKKSDPN 
      SVKSPGNRK RVLENSDNV ENVSKRPKS ELLSDRFRK 
      MMETTSRHM DALDNRDNE EQEKYFEKL EKKEQMENK 
      MINTFKVAC KAVRCLQCK YTNFSASQF CKENKHPLK 
      VFDAMKRFF KCGDCGNRI TSLEVVPTV VCKNCGSGK 
      WERTGMMRE KTVDVGPTL SIRGGEQTF VNSVITDSN 
      LNLLVCRKF NEVGSRMLS RGFIQLEKR HAAIYKRVK 
      SLLPRRESE RRVHPLSRH CDILQAVET RISMLNMTY 
      MKYIENNLL CFIPGKVLD EMINVLSFV ESDCTPPRT 
      HQLLQELRD LSSMAMEHF DEHILPRVK EHKEQMDEK 
      RQAFIASFS GSPSTLKPA QPFIVQQVN DLQKIKKQY 
      KTYKHHIAY LNQNTQKLI QKMKKQNKR LKAQSVRIR 
      DQERKIQEQ SAKIQEQET ALADIKKHM DEWDQKYKD 
      LTNELVRAR DYILLKASS PKASSSCTP PPPKLCRPS 
      IRPRVSHLL FRNYGQIHL DLERKRKSN LLPEIPVKV 
      TRTQGDEDD KSIEQLLPL KEPDKSGDS DGKGNFSFL 
      IENLIKTPL TAIKSRKRK MAEEIDLK
    CDS sequence
    • >TC011513 RA
      atgagcaat acgtcgttc attttgtac atacctgaa 
      gttgcccta gaaaagatc ttctcattc ttgacctac 
      gatgaaatt gccaaaaac cgactggac ttgttagac 
      agtctcctc tcagtggcc accgaagaa ctgaaaagg 
      gataacgca ccggtaagg accctaacc gaagcagac 
      ctcttcggc aaagacgag gacgaaaaa ccaaaaaac 
      ccgtccaaa agctccatt catactgga gacaccgac 
      tcatcagac gacgaagac aataagtac cacgaagag 
      cgcaaatac aatgattac gggcgtgaa attaagcac 
      ttattggag tcgaatcac tcggcccca aatagcccc 
      atttctcag ccggaagtc tcgtggaaa aatcagact 
      gttaaaccg aagatattg cccaaaata caagacgct 
      caaagtgac cctgttttc ggattaaga atcgttaat 
      cctaccata tctttcaat acgttacaa gacagaatg 
      gttggacgt gaagctgtt gccttttca agagttaaa 
      aagtttgtt tcggggaac atttcggac aaaaattgg 
      gtaatagcg ggggttatt gccgggaaa agctcagtt 
      aaaacgtcc cagaaaggc aaccagttc ataatctgg 
      tgtctcagt gacttgaaa aatgatatt aaaaccgta 
      tcagttctc ttgttcggg agtgcatat aaacaactg 
      tggaaaaca gcaacaggg actgtggtc ggaatcctg 
      aacccaaca gttttagag acgagggat aattccaga 
      gatgaagct actttaagt gttgacaat agtcaaaaa 
      gtaatgatt atgggacag tctaaagac ttgggtact 
      tgcaaaagt acgaaaaaa agcggggcc caatgcact 
      aatattgtc aatacaagt cattgcgaa tattgcatt 
      tatcacatc aaacaagag taccaaaaa tgcagcaaa 
      cgcgcagaa ttacagtcg agtttcgtt ggaaaaggc 
      ttgacagcc cttaggaac aaggtttta ggcaaaaat 
      gaagttttt tatgctgga aaatcgtac acggcaatt 
      ccagccaaa cgaagcaag aagttagaa caaaaagag 
      aagaatctt cttcaagct cttagtggt aatgcacca 
      ctgaccaga aaacccacc tctacaaaa aaaacttca 
      aaagttgct gcaaatatt gaagttaca caacgtcag 
      aggctacga gatttgagt ttattggat aggttgggc 
      ggatatggt gttaaaact gatttcaaa ggcacacat 
      tcggcagaa gtcacccta gaaagttcg aagaaaact 
      gcaattgaa gttatttcg aaactgaaa gccgttgca 
      aaaactaca gacaaggtg gaacctgtg gcaaagctt 
      caagaagtc acagcaaca cattcggca gaagttact 
      ttagaaagt tcgaaaaaa actgcactt gaagctatc 
      tcaaaactg aaatctgca acaaattcg aagaagatg 
      gaacctgtc ttgaggcct caagaagtc atttcggaa 
      aatacaaag aaaacttca attgaaatt atttcgaaa 
      gtggatgtt ccagtgctg ttaggtagt gacaaggga 
      acaattgat ttgggggcg tcagttacc ccccaacaa 
      atcaatcga gcaaagtta aatgctctg aaatatgtg 
      caaaaaaac ggccctttg aaaaaatcg gaccccaac 
      agcgtaaag tcgccagga aacagaaaa cgggttttg 
      gaaaactcc gataacgta gagaacgtt tcaaagaga 
      ccaaaaagc gagttactt tcagaccgg tttagaaaa 
      atgatggag acaacttcg agacacatg gacgcgctc 
      gacaaccgt gataatgaa gagcaagaa aaatatttt 
      gaaaagttg gaaaagaag gaacaaatg gagaacaaa 
      atgatcaat acgtttaaa gtggcgtgt aaagctgtt 
      aggtgtttg cagtgtaaa tacacaaat ttttcggca 
      tcgcagttt tgtaaagag aacaaacac ccgttgaaa 
      gtttttgat gccatgaag aggtttttc aaatgtggg 
      gattgtggg aataggatt acgtcgctg gaggttgtt 
      ccgacagtt gtttgtaag aattgtggt tcagggaag 
      tgggaaagg actgggatg atgagggag aagacagtg 
      gatgtgggg ccgacgttg tcaatcagg ggcggagaa 
      caaacgttc gttaattcg gttattaca gactccaac 
      ttgaatctg ctggtttgt agaaaattc aatgaggta 
      ggaagtcgt atgctatca agaggattc atccaactg 
      gaaaagaga catgcagcc atttacaaa agagtcaaa 
      tccctactg ccacgaaga gaatctgag cgaagagtt 
      cacccatta tctcgccac tgtgacatt cttcaagcc 
      gtcgaaact cgaatcagc atgctaaac atgacatac 
      atgaaatac atcgagaat aatttatta tgttttata 
      cctggaaaa gttttagac gaaatgata aacgtccta 
      agcttcgtc gaaagcgac tgcactccc ccacgtact 
      caccagctg ttacaagaa ctccgcgac ctcagcagc 
      atggcaatg gagcatttc gacgaacac atcctgccc 
      cgtgtcaaa gaacacaaa gaacaaatg gacgaaaaa 
      cgtcaagct ttcattgcc tcgttctcc ggaagcccc 
      tcgaccctg aaaccggcg caaccgttc atcgtccaa 
      caagtcaac gacttgcaa aaaatcaaa aagcaatac 
      aaaacctac aagcaccac atcgcctat ctcaaccaa 
      aacacgcag aagctgatc cagaagatg aagaagcag 
      aacaagcgc ctcaaagcc cagtcggtg cgcatccgc 
      gaccaggag cggaaaatc caagaacag tccgccaaa 
      atccaagag caggagacc gctctcgcc gatatcaaa 
      aagcacatg gacgagtgg gaccagaag tacaaggac 
      ctgaccaac gagctggtg cgtgccagg gactacatc 
      ctcctgaag gcgtcgtct ccgaaggcg agctcctcg 
      tgcacgccg ccgccgccg aaattgtgc agaccgagc 
      atcagaccg cgagtttca cacttgctt ttccgaaat 
      tacggacaa attcatctg gatttggag cgcaaacgc 
      aagtccaac ttgttgccg gaaattccg gttaaagtg 
      acgaggacc caaggtgac gaggacgac aagagcata 
      gagcagctc cttccgctc aaagagccc gacaaaagc 
      ggcgacagc gacgggaaa ggcaacttc agttttttg 
      atcgaaaat ttgatcaaa accccgcta acggccatc 
      aagtcgcgg aagcggaaa atggcagaa gagatcgac 
      ttgaaataa
    mRNA sequence
    • >TC011513 RA
      tacacgcac ttgacgaaa tggtggtgt atttatttt 
      tgttttgtg aaattaaac aatataata tttcgtgaa 
      attgaactc attcaagtg atttgcaag aaccccagc 
      cttcactac ccgagtaat tcagctcat tgcttgaga 
      caatgagca atacgtcgt tcattttgt acatacctg 
      aagttgccc tagaaaaga tcttctcat tcttgacct 
      acgatgaaa ttgccaaaa accgactgg acttgttag 
      acagtctcc tctcagtgg ccaccgaag aactgaaaa 
      gggataacg caccggtaa ggaccctaa ccgaagcag 
      acctcttcg gcaaagacg aggacgaaa aaccaaaaa 
      acccgtcca aaagctcca ttcatactg gagacaccg 
      actcatcag acgacgaag acaataagt accacgaag 
      agcgcaaat acaatgatt acgggcgtg aaattaagc 
      acttattgg agtcgaatc actcggccc caaatagcc 
      ccatttctc agccggaag tctcgtgga aaaatcaga 
      ctgttaaac cgaagatat tgcccaaaa tacaagacg 
      ctcaaagtg accctgttt tcggattaa gaatcgtta 
      atcctacca tatctttca atacgttac aagacagaa 
      tggttggac gtgaagctg ttgcctttt caagagtta 
      aaaagtttg tttcgggga acatttcgg acaaaaatt 
      gggtaatag cgggggtta ttgccggga aaagctcag 
      ttaaaacgt cccagaaag gcaaccagt tcataatct 
      ggtgtctca gtgacttga aaaatgata ttaaaaccg 
      tatcagttc tcttgttcg ggagtgcat ataaacaac 
      tgtggaaaa cagcaacag ggactgtgg tcggaatcc 
      tgaacccaa cagttttag agacgaggg ataattcca 
      gagatgaag ctactttaa gtgttgaca atagtcaaa 
      aagtaatga ttatgggac agtctaaag acttgggta 
      cttgcaaaa gtacgaaaa aaagcgggg cccaatgca 
      ctaatattg tcaatacaa gtcattgcg aatattgca 
      tttatcaca tcaaacaag agtaccaaa aatgcagca 
      aacgcgcag aattacagt cgagtttcg ttggaaaag 
      gcttgacag cccttagga acaaggttt taggcaaaa 
      atgaagttt tttatgctg gaaaatcgt acacggcaa 
      ttccagcca aacgaagca agaagttag aacaaaaag 
      agaagaatc ttcttcaag ctcttagtg gtaatgcac 
      cactgacca gaaaaccca cctctacaa aaaaaactt 
      caaaagttg ctgcaaata ttgaagtta cacaacgtc 
      agaggctac gagatttga gtttattgg ataggttgg 
      gcggatatg gtgttaaaa ctgatttca aaggcacac 
      attcggcag aagtcaccc tagaaagtt cgaagaaaa 
      ctgcaattg aagttattt cgaaactga aagccgttg 
      caaaaacta cagacaagg tggaacctg tggcaaagc 
      ttcaagaag tcacagcaa cacattcgg cagaagtta 
      ctttagaaa gttcgaaaa aaactgcac ttgaagcta 
      tctcaaaac tgaaatctg caacaaatt cgaagaaga 
      tggaacctg tcttgaggc ctcaagaag tcatttcgg 
      aaaatacaa agaaaactt caattgaaa ttatttcga 
      aagtggatg ttccagtgc tgttaggta gtgacaagg 
      gaacaattg atttggggg cgtcagtta ccccccaac 
      aaatcaatc gagcaaagt taaatgctc tgaaatatg 
      tgcaaaaaa acggccctt tgaaaaaat cggacccca 
      acagcgtaa agtcgccag gaaacagaa aacgggttt 
      tggaaaact ccgataacg tagagaacg tttcaaaga 
      gaccaaaaa gcgagttac tttcagacc ggtttagaa 
      aaatgatgg agacaactt cgagacaca tggacgcgc 
      tcgacaacc gtgataatg aagagcaag aaaaatatt 
      ttgaaaagt tggaaaaga aggaacaaa tggagaaca 
      aaatgatca atacgttta aagtggcgt gtaaagctg 
      ttaggtgtt tgcagtgta aatacacaa atttttcgg 
      catcgcagt tttgtaaag agaacaaac acccgttga 
      aagtttttg atgccatga agaggtttt tcaaatgtg 
      gggattgtg ggaatagga ttacgtcgc tggaggttg 
      ttccgacag ttgtttgta agaattgtg gttcaggga 
      agtgggaaa ggactggga tgatgaggg agaagacag 
      tggatgtgg ggccgacgt tgtcaatca ggggcggag 
      aacaaacgt tcgttaatt cggttatta cagactcca 
      acttgaatc tgctggttt gtagaaaat tcaatgagg 
      taggaagtc gtatgctat caagaggat tcatccaac 
      tggaaaaga gacatgcag ccatttaca aaagagtca 
      aatccctac tgccacgaa gagaatctg agcgaagag 
      ttcacccat tatctcgcc actgtgaca ttcttcaag 
      ccgtcgaaa ctcgaatca gcatgctaa acatgacat 
      acatgaaat acatcgaga ataatttat tatgtttta 
      tacctggaa aagttttag acgaaatga taaacgtcc 
      taagcttcg tcgaaagcg actgcactc ccccacgta 
      ctcaccagc tgttacaag aactccgcg acctcagca 
      gcatggcaa tggagcatt tcgacgaac acatcctgc 
      cccgtgtca aagaacaca aagaacaaa tggacgaaa 
      aacgtcaag ctttcattg cctcgttct ccggaagcc 
      cctcgaccc tgaaaccgg cgcaaccgt tcatcgtcc 
      aacaagtca acgacttgc aaaaaatca aaaagcaat 
      acaaaacct acaagcacc acatcgcct atctcaacc 
      aaaacacgc agaagctga tccagaaga tgaagaagc 
      agaacaagc gcctcaaag cccagtcgg tgcgcatcc 
      gcgaccagg agcggaaaa tccaagaac agtccgcca 
      aaatccaag agcaggaga ccgctctcg ccgatatca 
      aaaagcaca tggacgagt gggaccaga agtacaagg 
      acctgacca acgagctgg tgcgtgcca gggactaca 
      tcctcctga aggcgtcgt ctccgaagg cgagctcct 
      cgtgcacgc cgccgccgc cgaaattgt gcagaccga 
      gcatcagac cgcgagttt cacacttgc ttttccgaa 
      attacggac aaattcatc tggatttgg agcgcaaac 
      gcaagtcca acttgttgc cggaaattc cggttaaag 
      tgacgagga cccaaggtg acgaggacg acaagagca 
      tagagcagc tccttccgc tcaaagagc ccgacaaaa 
      gcggcgaca gcgacggga aaggcaact tcagttttt 
      tgatcgaaa atttgatca aaaccccgc taacggcca 
      tcaagtcgc ggaagcgga aaatggcag aagagatcg 
      acttgaaat aaatttttt atattaatt aatctccaa 
      gtcccattg agtaagatt ttgttatgt ttttaaggg 
      agaattacg tcaaaaaat attaaaacc tttattaca 
      gtaagaaat aaatacaat taaaaaagg taagtcgag 
      ttagaaagg aagtggtcg ttaaaatgg tgtaataaa 
      atacatact gtaagttct caacacaag tttggttaa 
      taaacgtaa taaaaggct ggttttagt gttataata 
      ctctatcct ccgcattgt taatataaa taactctat 
      taataaaat aaatatgtt atctagtat gttacataa 
      tgcctttgc tatattatt atggaaaca agttctttc 
      taccgcgaa acgaaaagg cagatttag tgcaccaat 
      acaatgtct cctaacgtg aacatgaac taatgaacg 
      ctaatatgc cgataaata cttaaacct gaagtcaca 
      gtagaaaat ctaataatg taaatatta aaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY