Status
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Gene information
General information
TC011513 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3822038..3826828
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011513
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09947

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011513 RA
      MSNTSFILY IPEVALEKI FSFLTYDEI AKNRLDLLD 
SLLSVATEE LKRDNAPVR TLTEADLFG KDEDEKPKN 
PSKSSIHTG DTDSSDDED NKYHEERKY NDYGREIKH 
LLESNHSAP NSPISQPEV SWKNQTVKP KILPKIQDA 
QSDPVFGLR IVNPTISFN TLQDRMVGR EAVAFSRVK 
KFVSGNISD KNWVIAGVI AGKSSVKTS QKGNQFIIW 
CLSDLKNDI KTVSVLLFG SAYKQLWKT ATGTVVGIL 
NPTVLETRD NSRDEATLS VDNSQKVMI MGQSKDLGT 
CKSTKKSGA QCTNIVNTS HCEYCIYHI KQEYQKCSK 
RAELQSSFV GKGLTALRN KVLGKNEVF YAGKSYTAI 
PAKRSKKLE QKEKNLLQA LSGNAPLTR KPTSTKKTS 
KVAANIEVT QRQRLRDLS LLDRLGGYG VKTDFKGTH 
SAEVTLESS KKTAIEVIS KLKAVAKTT DKVEPVAKL 
QEVTATHSA EVTLESSKK TALEAISKL KSATNSKKM 
EPVLRPQEV ISENTKKTS IEIISKVDV PVLLGSDKG 
TIDLGASVT PQQINRAKL NALKYVQKN GPLKKSDPN 
SVKSPGNRK RVLENSDNV ENVSKRPKS ELLSDRFRK 
MMETTSRHM DALDNRDNE EQEKYFEKL EKKEQMENK 
MINTFKVAC KAVRCLQCK YTNFSASQF CKENKHPLK 
VFDAMKRFF KCGDCGNRI TSLEVVPTV VCKNCGSGK 
WERTGMMRE KTVDVGPTL SIRGGEQTF VNSVITDSN 
LNLLVCRKF NEVGSRMLS RGFIQLEKR HAAIYKRVK 
SLLPRRESE RRVHPLSRH CDILQAVET RISMLNMTY 
MKYIENNLL CFIPGKVLD EMINVLSFV ESDCTPPRT 
HQLLQELRD LSSMAMEHF DEHILPRVK EHKEQMDEK 
RQAFIASFS GSPSTLKPA QPFIVQQVN DLQKIKKQY 
KTYKHHIAY LNQNTQKLI QKMKKQNKR LKAQSVRIR 
DQERKIQEQ SAKIQEQET ALADIKKHM DEWDQKYKD 
LTNELVRAR DYILLKASS PKASSSCTP PPPKLCRPS 
IRPRVSHLL FRNYGQIHL DLERKRKSN LLPEIPVKV 
TRTQGDEDD KSIEQLLPL KEPDKSGDS DGKGNFSFL 
IENLIKTPL TAIKSRKRK MAEEIDLK
    CDS sequence
    • >TC011513 RA
      atgagcaat acgtcgttc attttgtac atacctgaa 
gttgcccta gaaaagatc ttctcattc ttgacctac 
gatgaaatt gccaaaaac cgactggac ttgttagac 
agtctcctc tcagtggcc accgaagaa ctgaaaagg 
gataacgca ccggtaagg accctaacc gaagcagac 
ctcttcggc aaagacgag gacgaaaaa ccaaaaaac 
ccgtccaaa agctccatt catactgga gacaccgac 
tcatcagac gacgaagac aataagtac cacgaagag 
cgcaaatac aatgattac gggcgtgaa attaagcac 
ttattggag tcgaatcac tcggcccca aatagcccc 
atttctcag ccggaagtc tcgtggaaa aatcagact 
gttaaaccg aagatattg cccaaaata caagacgct 
caaagtgac cctgttttc ggattaaga atcgttaat 
cctaccata tctttcaat acgttacaa gacagaatg 
gttggacgt gaagctgtt gccttttca agagttaaa 
aagtttgtt tcggggaac atttcggac aaaaattgg 
gtaatagcg ggggttatt gccgggaaa agctcagtt 
aaaacgtcc cagaaaggc aaccagttc ataatctgg 
tgtctcagt gacttgaaa aatgatatt aaaaccgta 
tcagttctc ttgttcggg agtgcatat aaacaactg 
tggaaaaca gcaacaggg actgtggtc ggaatcctg 
aacccaaca gttttagag acgagggat aattccaga 
gatgaagct actttaagt gttgacaat agtcaaaaa 
gtaatgatt atgggacag tctaaagac ttgggtact 
tgcaaaagt acgaaaaaa agcggggcc caatgcact 
aatattgtc aatacaagt cattgcgaa tattgcatt 
tatcacatc aaacaagag taccaaaaa tgcagcaaa 
cgcgcagaa ttacagtcg agtttcgtt ggaaaaggc 
ttgacagcc cttaggaac aaggtttta ggcaaaaat 
gaagttttt tatgctgga aaatcgtac acggcaatt 
ccagccaaa cgaagcaag aagttagaa caaaaagag 
aagaatctt cttcaagct cttagtggt aatgcacca 
ctgaccaga aaacccacc tctacaaaa aaaacttca 
aaagttgct gcaaatatt gaagttaca caacgtcag 
aggctacga gatttgagt ttattggat aggttgggc 
ggatatggt gttaaaact gatttcaaa ggcacacat 
tcggcagaa gtcacccta gaaagttcg aagaaaact 
gcaattgaa gttatttcg aaactgaaa gccgttgca 
aaaactaca gacaaggtg gaacctgtg gcaaagctt 
caagaagtc acagcaaca cattcggca gaagttact 
ttagaaagt tcgaaaaaa actgcactt gaagctatc 
tcaaaactg aaatctgca acaaattcg aagaagatg 
gaacctgtc ttgaggcct caagaagtc atttcggaa 
aatacaaag aaaacttca attgaaatt atttcgaaa 
gtggatgtt ccagtgctg ttaggtagt gacaaggga 
acaattgat ttgggggcg tcagttacc ccccaacaa 
atcaatcga gcaaagtta aatgctctg aaatatgtg 
caaaaaaac ggccctttg aaaaaatcg gaccccaac 
agcgtaaag tcgccagga aacagaaaa cgggttttg 
gaaaactcc gataacgta gagaacgtt tcaaagaga 
ccaaaaagc gagttactt tcagaccgg tttagaaaa 
atgatggag acaacttcg agacacatg gacgcgctc 
gacaaccgt gataatgaa gagcaagaa aaatatttt 
gaaaagttg gaaaagaag gaacaaatg gagaacaaa 
atgatcaat acgtttaaa gtggcgtgt aaagctgtt 
aggtgtttg cagtgtaaa tacacaaat ttttcggca 
tcgcagttt tgtaaagag aacaaacac ccgttgaaa 
gtttttgat gccatgaag aggtttttc aaatgtggg 
gattgtggg aataggatt acgtcgctg gaggttgtt 
ccgacagtt gtttgtaag aattgtggt tcagggaag 
tgggaaagg actgggatg atgagggag aagacagtg 
gatgtgggg ccgacgttg tcaatcagg ggcggagaa 
caaacgttc gttaattcg gttattaca gactccaac 
ttgaatctg ctggtttgt agaaaattc aatgaggta 
ggaagtcgt atgctatca agaggattc atccaactg 
gaaaagaga catgcagcc atttacaaa agagtcaaa 
tccctactg ccacgaaga gaatctgag cgaagagtt 
cacccatta tctcgccac tgtgacatt cttcaagcc 
gtcgaaact cgaatcagc atgctaaac atgacatac 
atgaaatac atcgagaat aatttatta tgttttata 
cctggaaaa gttttagac gaaatgata aacgtccta 
agcttcgtc gaaagcgac tgcactccc ccacgtact 
caccagctg ttacaagaa ctccgcgac ctcagcagc 
atggcaatg gagcatttc gacgaacac atcctgccc 
cgtgtcaaa gaacacaaa gaacaaatg gacgaaaaa 
cgtcaagct ttcattgcc tcgttctcc ggaagcccc 
tcgaccctg aaaccggcg caaccgttc atcgtccaa 
caagtcaac gacttgcaa aaaatcaaa aagcaatac 
aaaacctac aagcaccac atcgcctat ctcaaccaa 
aacacgcag aagctgatc cagaagatg aagaagcag 
aacaagcgc ctcaaagcc cagtcggtg cgcatccgc 
gaccaggag cggaaaatc caagaacag tccgccaaa 
atccaagag caggagacc gctctcgcc gatatcaaa 
aagcacatg gacgagtgg gaccagaag tacaaggac 
ctgaccaac gagctggtg cgtgccagg gactacatc 
ctcctgaag gcgtcgtct ccgaaggcg agctcctcg 
tgcacgccg ccgccgccg aaattgtgc agaccgagc 
atcagaccg cgagtttca cacttgctt ttccgaaat 
tacggacaa attcatctg gatttggag cgcaaacgc 
aagtccaac ttgttgccg gaaattccg gttaaagtg 
acgaggacc caaggtgac gaggacgac aagagcata 
gagcagctc cttccgctc aaagagccc gacaaaagc 
ggcgacagc gacgggaaa ggcaacttc agttttttg 
atcgaaaat ttgatcaaa accccgcta acggccatc 
aagtcgcgg aagcggaaa atggcagaa gagatcgac 
ttgaaataa
    mRNA sequence
    • >TC011513 RA
      tacacgcac ttgacgaaa tggtggtgt atttatttt 
tgttttgtg aaattaaac aatataata tttcgtgaa 
attgaactc attcaagtg atttgcaag aaccccagc 
cttcactac ccgagtaat tcagctcat tgcttgaga 
caatgagca atacgtcgt tcattttgt acatacctg 
aagttgccc tagaaaaga tcttctcat tcttgacct 
acgatgaaa ttgccaaaa accgactgg acttgttag 
acagtctcc tctcagtgg ccaccgaag aactgaaaa 
gggataacg caccggtaa ggaccctaa ccgaagcag 
acctcttcg gcaaagacg aggacgaaa aaccaaaaa 
acccgtcca aaagctcca ttcatactg gagacaccg 
actcatcag acgacgaag acaataagt accacgaag 
agcgcaaat acaatgatt acgggcgtg aaattaagc 
acttattgg agtcgaatc actcggccc caaatagcc 
ccatttctc agccggaag tctcgtgga aaaatcaga 
ctgttaaac cgaagatat tgcccaaaa tacaagacg 
ctcaaagtg accctgttt tcggattaa gaatcgtta 
atcctacca tatctttca atacgttac aagacagaa 
tggttggac gtgaagctg ttgcctttt caagagtta 
aaaagtttg tttcgggga acatttcgg acaaaaatt 
gggtaatag cgggggtta ttgccggga aaagctcag 
ttaaaacgt cccagaaag gcaaccagt tcataatct 
ggtgtctca gtgacttga aaaatgata ttaaaaccg 
tatcagttc tcttgttcg ggagtgcat ataaacaac 
tgtggaaaa cagcaacag ggactgtgg tcggaatcc 
tgaacccaa cagttttag agacgaggg ataattcca 
gagatgaag ctactttaa gtgttgaca atagtcaaa 
aagtaatga ttatgggac agtctaaag acttgggta 
cttgcaaaa gtacgaaaa aaagcgggg cccaatgca 
ctaatattg tcaatacaa gtcattgcg aatattgca 
tttatcaca tcaaacaag agtaccaaa aatgcagca 
aacgcgcag aattacagt cgagtttcg ttggaaaag 
gcttgacag cccttagga acaaggttt taggcaaaa 
atgaagttt tttatgctg gaaaatcgt acacggcaa 
ttccagcca aacgaagca agaagttag aacaaaaag 
agaagaatc ttcttcaag ctcttagtg gtaatgcac 
cactgacca gaaaaccca cctctacaa aaaaaactt 
caaaagttg ctgcaaata ttgaagtta cacaacgtc 
agaggctac gagatttga gtttattgg ataggttgg 
gcggatatg gtgttaaaa ctgatttca aaggcacac 
attcggcag aagtcaccc tagaaagtt cgaagaaaa 
ctgcaattg aagttattt cgaaactga aagccgttg 
caaaaacta cagacaagg tggaacctg tggcaaagc 
ttcaagaag tcacagcaa cacattcgg cagaagtta 
ctttagaaa gttcgaaaa aaactgcac ttgaagcta 
tctcaaaac tgaaatctg caacaaatt cgaagaaga 
tggaacctg tcttgaggc ctcaagaag tcatttcgg 
aaaatacaa agaaaactt caattgaaa ttatttcga 
aagtggatg ttccagtgc tgttaggta gtgacaagg 
gaacaattg atttggggg cgtcagtta ccccccaac 
aaatcaatc gagcaaagt taaatgctc tgaaatatg 
tgcaaaaaa acggccctt tgaaaaaat cggacccca 
acagcgtaa agtcgccag gaaacagaa aacgggttt 
tggaaaact ccgataacg tagagaacg tttcaaaga 
gaccaaaaa gcgagttac tttcagacc ggtttagaa 
aaatgatgg agacaactt cgagacaca tggacgcgc 
tcgacaacc gtgataatg aagagcaag aaaaatatt 
ttgaaaagt tggaaaaga aggaacaaa tggagaaca 
aaatgatca atacgttta aagtggcgt gtaaagctg 
ttaggtgtt tgcagtgta aatacacaa atttttcgg 
catcgcagt tttgtaaag agaacaaac acccgttga 
aagtttttg atgccatga agaggtttt tcaaatgtg 
gggattgtg ggaatagga ttacgtcgc tggaggttg 
ttccgacag ttgtttgta agaattgtg gttcaggga 
agtgggaaa ggactggga tgatgaggg agaagacag 
tggatgtgg ggccgacgt tgtcaatca ggggcggag 
aacaaacgt tcgttaatt cggttatta cagactcca 
acttgaatc tgctggttt gtagaaaat tcaatgagg 
taggaagtc gtatgctat caagaggat tcatccaac 
tggaaaaga gacatgcag ccatttaca aaagagtca 
aatccctac tgccacgaa gagaatctg agcgaagag 
ttcacccat tatctcgcc actgtgaca ttcttcaag 
ccgtcgaaa ctcgaatca gcatgctaa acatgacat 
acatgaaat acatcgaga ataatttat tatgtttta 
tacctggaa aagttttag acgaaatga taaacgtcc 
taagcttcg tcgaaagcg actgcactc ccccacgta 
ctcaccagc tgttacaag aactccgcg acctcagca 
gcatggcaa tggagcatt tcgacgaac acatcctgc 
cccgtgtca aagaacaca aagaacaaa tggacgaaa 
aacgtcaag ctttcattg cctcgttct ccggaagcc 
cctcgaccc tgaaaccgg cgcaaccgt tcatcgtcc 
aacaagtca acgacttgc aaaaaatca aaaagcaat 
acaaaacct acaagcacc acatcgcct atctcaacc 
aaaacacgc agaagctga tccagaaga tgaagaagc 
agaacaagc gcctcaaag cccagtcgg tgcgcatcc 
gcgaccagg agcggaaaa tccaagaac agtccgcca 
aaatccaag agcaggaga ccgctctcg ccgatatca 
aaaagcaca tggacgagt gggaccaga agtacaagg 
acctgacca acgagctgg tgcgtgcca gggactaca 
tcctcctga aggcgtcgt ctccgaagg cgagctcct 
cgtgcacgc cgccgccgc cgaaattgt gcagaccga 
gcatcagac cgcgagttt cacacttgc ttttccgaa 
attacggac aaattcatc tggatttgg agcgcaaac 
gcaagtcca acttgttgc cggaaattc cggttaaag 
tgacgagga cccaaggtg acgaggacg acaagagca 
tagagcagc tccttccgc tcaaagagc ccgacaaaa 
gcggcgaca gcgacggga aaggcaact tcagttttt 
tgatcgaaa atttgatca aaaccccgc taacggcca 
tcaagtcgc ggaagcgga aaatggcag aagagatcg 
acttgaaat aaatttttt atattaatt aatctccaa 
gtcccattg agtaagatt ttgttatgt ttttaaggg 
agaattacg tcaaaaaat attaaaacc tttattaca 
gtaagaaat aaatacaat taaaaaagg taagtcgag 
ttagaaagg aagtggtcg ttaaaatgg tgtaataaa 
atacatact gtaagttct caacacaag tttggttaa 
taaacgtaa taaaaggct ggttttagt gttataata 
ctctatcct ccgcattgt taatataaa taactctat 
taataaaat aaatatgtt atctagtat gttacataa 
tgcctttgc tatattatt atggaaaca agttctttc 
taccgcgaa acgaaaagg cagatttag tgcaccaat 
acaatgtct cctaacgtg aacatgaac taatgaacg 
ctaatatgc cgataaata cttaaacct gaagtcaca 
gtagaaaat ctaataatg taaatatta aaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY