Status
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Gene information
General information
TC011551 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3806410..3817025
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011551
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09945

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011551 RA
      MSENSVGAM PGVAGAGER RLRQLEALF LGGPVLAKG 
      QSFSIETLI DILIVLYDE CCNSSLRKE KTVSDFIEY 
      VKPVASSVK SLRLTREDF EIIKVIGRG AFGEVCVVK 
      FRGSEKVFA MKILNKWEM LKRAETACF KEERDVLVY 
      GDRRWITHL HYAFQDESN LYLVMDYYC GGDLLTLLS 
      KFEDRLPED MARFYIAEM VLAIDSIHK LGYVHRDIK 
      PDNVLLDAN GHIRLADFG SCLKLNDDG TVQSNVAVG 
      TPDYISPEI LRAMEDGQG KYGPECDWW SLGVCMYEM 
      LFGETPFYA ESLVETYGK IMNHKNCFD FATDVDVSD 
      AAKDLIKQL ICSQEFRLG QNGIQDFKN HPWFEGVDW 
      DGIRDSNAP YIPEVSSPT DTSNFDVDD ADIRLSDAM 
      PPTANNAFT GLHLPFVGF TFTQGSCIC DLSNLYTNI 
      TNNLADKQS KRNNGEIVS LNLNEEKEM DKRMSPDGT 
      RKLQDEINT LTKRNCELE SQLRSFESG HSKDLFIDT 
      LDGAENAKV KELEKLNRI LKQEKEEAM KEKLDLHEK 
      LKFQDKELK DALAQKKLA MAEYTEVSD KLSELRQQK 
      QKFSRQVRD KEEELEAVM QKVDSLRND IRRAEKLRR 
      ELESRVDEA LAEATKERK LRERSEEYC RQMQAESDR 
      LRVRSELGP RDQQDNLRL KAELEKLEV QYNESLAQQ 
      QARFNLELS SLREQLHEA ESHRELLER EVTELKEKQ 
      EKHRVEALS DSEQMIAEL NRRSERDKQ LLLDDNHKL 
      TANVEFLTE SVERLQSER ATLEAEYEQ LRNKQEALG 
      QWESQLAEI IQWVSDEKD ARAYLQALA TKMTEELDY 
      LKHAGVSNA MGGTDKNWR NRRSQKLDK MELLNLQSS 
      LQSEIQAKQ AISEELSKT REALLEAQK DLRDSRQRN 
      EAFALDLKR KEKQIKELQ SRLDSEGFL ERPSSQMSY 
      LEQFLKENN QLQVQSQTY DNVPPLLYQ GGSIESEEG 
      DIEDNRALS LTSSKSNLS ELSMHDSQS PMKHKNHQF 
      LVRTFSSPT KCNHCTSLM VGLMRQGMV CEICGFTCH 
      TSCCPHVPA ICPLPSDQT KRPLGIDPT RGIGTAYEG 
      YVKVPKQGG VKKGWIRQF VVVCDFKLF LYDISADRN 
      ALPSVHVAQ VLDMRDPLF SVSSVKDSD VIHANKKDI 
      PCIFKITTS LMEPPGPRN STLMLADSE AEKNKWVVA 
      LSELHRIMK RNNLPNTTV LLARELIDN SLTLLRNTL 
      SAAIIDPDR IVLGAEEGL HCVDLDRSE IAKIGEGKK 
      IFQLEYISE EHLLVVVSG KQRYVRLVP IRALDGDET 
      EWIKVAESK GCSVLTVGP VVRAPLTFC LCVAIKKQQ 
      NSSTVIIYE ITRTRTRHH RIRDINLGA PVQSLQLLS 
      DGRLCIGSP SNFTICNLQ TEQPLFSLV SSENPLHAT 
      LACNAVEAL RVIELPRHE YLLVFGTLA AYVDRHGRK 
      SRDREIMYP AVPTAICYR DGHLIVYSE THIDIFNCA 
      TGEWVQTLN IKKAKPLND SGALSLCIL NDLTHLLYL 
      SNIHQRELI NLDNVVTLD RDGRTLQRP RRRFSLREG 
      NRSQRINTD RRSKLISAP TNFNHISHM GPGEGIQRQ 
      RLIDLSTNL VDTPDQSSY GPQVQPRQP TKMAPLPPK 
      HGERKRLEI SNPIRGPPF PPAYNGAKR AAPPRPRDL 
      PPALPRPSD TSPSPDPAS IGSMSSLHD MIKGTEKGS 
      PRHSIASNN SSNLSTPPS PQADHHNSS SYDS
    CDS sequence
    • >TC011551 RA
      atgagtgaa aacagcgtg ggggccatg ccgggcgtg 
      gcaggggct ggggagcgg cgcctccgc cagctggag 
      gccctgttc cttgggggg cccgtgttg gcaaagggg 
      cagagcttc agcattgaa accctcatc gacatcctc 
      atcgttctg tacgatgag tgctgtaat tctagttta 
      cgtaaagag aaaacggtg tcggatttt atagaatat 
      gtgaaacct gtagcttct tcagtaaag tctcttagg 
      ctaactagg gaagatttc gagataatt aaagtcatt 
      ggtaggggg gctttcggt gaagtttgc gtagttaaa 
      tttagaggt tcagagaaa gtgttcgca atgaaaatt 
      cttaacaag tgggagatg cttaaaagg gcggaaact 
      gcgtgtttt aaggaggag agagatgtt ttagtgtat 
      ggagatagg aggtggatt acgcactta cattatgca 
      tttcaagat gagtctaat ttgtattta gtgatggat 
      tattattgt gggggcgat ttattgacg ctgttgagt 
      aaatttgag gaccgtctt ccggaagat atggcgcga 
      ttttacatt gctgaaatg gttttagcc atagattcg 
      atacataag cttggatat gtacacaga gacattaaa 
      cccgacaac gtgttattg gacgcaaat ggccatatt 
      agactagca gattttggt agttgtttg aaattaaat 
      gacgatgga acagtgcag tccaatgtt gctgttgga 
      acccctgat tatatctcc ccagagata ctcagagca 
      atggaggac ggtcaaggc aaatacggt ccagagtgt 
      gactggtgg tctctaggt gtttgcatg tatgaaatg 
      ttgtttggt gaaacaccg ttttacgcc gaaagcttg 
      gtcgaaact tacgggaag ataatgaac cataaaaac 
      tgttttgat tttgcgact gatgttgat gtgtctgat 
      gcggcgaaa gatttaatc aagcagtta atttgtagt 
      caagaattt cgtttagga cagaatgga atacaggat 
      tttaagaac catccatgg tttgagggt gtcgactgg 
      gatggtatt agagatagt aacgcgcct tatattccc 
      gaagtttcg agtccaaca gatacatca aattttgac 
      gtagacgat gctgatatc cgactgtcg gatgccatg 
      ccgccaact gccaataat gcctttacc ggacttcat 
      ttgcctttt gtaggcttt actttcact caaggaagt 
      tgtatatgc gacttgagt aacctttat acgaatata 
      acgaacaat ctagcggac aagcaaagt aaacgaaat 
      aatggcgaa attgtgagt ttgaatttg aacgaagaa 
      aaggaaatg gataagaga atgtcgcct gacggcact 
      aggaaattg caagatgaa attaacact ttaactaaa 
      cgtaactgc gagctggag tcgcaattg cgaagcttc 
      gaaagcggt cattcgaag gatttgttc attgatact 
      ttagatggt gctgaaaat gcaaaagtg aaagaattg 
      gaaaaactg aaccgtatt ttaaaacaa gagaaggaa 
      gaagcaatg aaggagaag ttagacctt catgaaaaa 
      ctcaaattt caagacaag gaattgaaa gatgcgttg 
      gcacaaaag aaactggcc atggccgag tatacggag 
      gtttccgat aagttgtcc gaactgcga caacagaag 
      cagaaattt agcagacag gttagggac aaggaggag 
      gaattggaa gccgttatg cagaaagtt gattcgctc 
      agaaacgac ataagacgg gcggagaaa ttgagaagg 
      gagttggaa tctcgcgtt gatgaagct ctagctgaa 
      gcaacgaag gaaaggaag ttgagggaa cgctcggag 
      gagtactgt cggcaaatg caggcagag tcggatcgg 
      ttgagagtg aggagtgag ctagggccc agagaccaa 
      caagataat ttaaggcta aaagcagaa ctggaaaaa 
      ttggaggtt cagtacaac gaaagtttg gcgcaacaa 
      caggccagg ttcaatctg gaattatcc tcacttagg 
      gaacaactc cacgaagcc gagtcccat cgcgaactc 
      ctcgaacgc gaagtgact gaacttaaa gaaaaacaa 
      gagaaacac cgtgtagag gcgctttcc gattctgaa 
      caaatgatt gccgaattg aatcgtcga agcgaacgc 
      gacaaacaa cttttgttg gacgataat cataaactt 
      accgcaaat gttgagttt ctgactgaa tctgtggag 
      cgcttgcaa tcggaacgc gcgacttta gaagcggaa 
      tacgaacag ttgaggaac aaacaagaa gcgttggga 
      caatgggag tcgcaactt gccgagatt atacagtgg 
      gttagtgat gaaaaggat gctagggct tatttgcaa 
      gcgctggct acgaaaatg actgaagaa ttggattat 
      ttgaaacat gctggtgtt tcaaatgcg atgggtggc 
      actgataag aattggagg aaccgaagg tcgcaaaaa 
      ctggacaag atggagctc ctcaacctt cagagctca 
      ctacagtcg gaaattcaa gccaagcaa gctataagc 
      gaggagttg agcaaaaca agagaggcc ttattagaa 
      gcgcaaaaa gatctgaga gattcgagg cagcgaaac 
      gaggccttt gccttggat ctcaaaagg aaggagaaa 
      caaatcaag gagttgcaa agtcgtctt gattcggaa 
      ggatttttg gaacgccct tcgtcccag atgtcttac 
      ttggaacaa ttcctcaag gaaaacaat cagttgcaa 
      gtccaaagt caaacttac gacaacgtg cccccactt 
      ttgtaccaa ggaggctcg atagagtct gaggaaggc 
      gatatcgaa gacaaccga gctttaagt ttgacgagt 
      tctaagagt aacttgtct gaactcagc atgcacgat 
      tcccagtct ccaatgaaa cacaaaaac catcaattt 
      ttggtgcga actttttct agtcctaca aaatgcaat 
      cactgcacg tcattgatg gtcggtttg atgcgacaa 
      ggaatggtg tgcgagatt tgcgggttt acctgtcac 
      acgtcgtgt tgtccacat gtgccggca atttgtccg 
      ttaccgtca gatcagacc aagagaccg ctggggatt 
      gaccccact agaggcata ggaactgct tacgagggc 
      tacgtcaaa gtgcccaag caaggaggt gtcaaaaag 
      ggttggatc agacaattt gttgttgtt tgtgacttc 
      aaactgttt ttatacgac atatcagca gatcgaaat 
      gccttacca agtgttcac gtagctcaa gttttagat 
      atgagggat ccactgttt agtgtttct agcgttaag 
      gattctgac gtcatccac gccaacaaa aaggacatt 
      ccttgcatc ttcaagatc accacctcc ttgatggag 
      cccccgggc ccgcgcaac tccaccctg atgttggcc 
      gactccgaa gccgaaaag aacaaatgg gtggtcgcc 
      ctctccgaa ctccatcga ataatgaag cgaaacaat 
      ttacccaac actacggtg ttactagcg cgcgaattg 
      atcgataac tccctaact ttgctaaga aacacacta 
      agtgctgcg attatcgac ccagatcgc atagtccta 
      ggcgccgaa gaaggactt cactgtgtc gatctagac 
      cgcagcgag attgcgaaa atcggcgaa ggcaaaaag 
      atcttccaa ctggagtat atcagtgag gagcacctg 
      ttggtcgtg gtcagtggt aaacagaga tacgtacgg 
      ttggtcccc atcagagca ctggacggc gatgagact 
      gaatggatc aaagtggcg gagagtaaa gggtgttcg 
      gtgctgacg gtggggcct gttgtgagg gcgccctta 
      acgttctgt ctatgtgtg gcgattaaa aaacagcaa 
      aattcctcg actgttatc atatacgag ataacgagg 
      acacggacg cgacaccat cgaattcgg gacataaat 
      ctgggggcg ccagttcag tccttgcag ttgttgtca 
      gatggccga ctttgtatc ggttctccg tcgaatttt 
      acgatttgt aatctacaa actgaacaa ccacttttt 
      tctttggtg agttcggag aatccacta catgccacg 
      ctggcttgt aatgcagtt gaggcacta agagtgata 
      gagttgcct cggcatgaa taccttcta gtttttggt 
      actttggcg gcttatgtt gatagacac ggccgcaaa 
      tctcgtgat agggaaata atgtatcca gcagtgccg 
      acagccata tgctataga gatggtcat ctcatcgtt 
      tatagtgag acccatatc gatattttt aactgtgca 
      acgggagaa tgggttcaa actcttaat atcaagaaa 
      gcaaagcct cttaatgat agtggggca ctttcgttg 
      tgcattcta aatgattta actcatctg ctgtatctg 
      tctaatata catcagcgt gaattaatt aatttagat 
      aatgtggtt acgttagat agagacgga cggactttg 
      cagagaccg cggaggcgg ttttcgctg agagagggg 
      aacaggtca caacgaata aacacggac cgccggtca 
      aaactgatt tcggcgccg acaaacttc aaccacata 
      agtcacatg ggccccggc gagggcata cagagacaa 
      agactgatt gatctttcg acgaactta gttgatact 
      cctgatcag tcctcctac ggtcctcag gtgcaacca 
      agacagcca accaagatg gcgcccctg cccccgaaa 
      cacggcgaa cgcaagcgt ctagaaata tcaaacccc 
      attcggggg ccccctttc ccgcccgcg tacaacggg 
      gccaagcgg gctgcgccc ccgcgcccg cgagatctt 
      ccaccggcc ttgccgcgc ccttccgac acgtcacct 
      tccccagat cctgcgagc attggaagc atgtcgtcg 
      ctgcacgat atgatcaag ggcacggag aaaggcagt 
      cctcgccac tcgatcgcg agcaataac agttcaaac 
      ctttcgact ccgccgtcg ccccaagca gatcatcat 
      aatagttcg agttacgat agctaa
    mRNA sequence
    • >TC011551 RA
      ttgtgactc attgtaccc aaattacac tcagtaggg 
      gagttgtaa aaatggctg ccaaaagtc atcaatgaa 
      gtgtatttt aaatggcca aataatgtt gattattta 
      ctattgatc tgcttgaca aagatggtg ctaacgcaa 
      cagtcaaat gagtgaaaa cagcgtggg ggccatgcc 
      gggcgtggc aggggctgg ggagcggcg cctccgcca 
      gctggaggc cctgttcct tggggggcc cgtgttggc 
      aaaggggca gagcttcag cattgaaac cctcatcga 
      catcctcat cgttctgta cgatgagtg ctgtaattc 
      tagtttacg taaagagaa aacggtgtc ggattttat 
      agaatatgt gaaacctgt agcttcttc agtaaagtc 
      tcttaggct aactaggga agatttcga gataattaa 
      agtcattgg taggggggc tttcggtga agtttgcgt 
      agttaaatt tagaggttc agagaaagt gttcgcaat 
      gaaaattct taacaagtg ggagatgct taaaagggc 
      ggaaactgc gtgttttaa ggaggagag agatgtttt 
      agtgtatgg agataggag gtggattac gcacttaca 
      ttatgcatt tcaagatga gtctaattt gtatttagt 
      gatggatta ttattgtgg gggcgattt attgacgct 
      gttgagtaa atttgagga ccgtcttcc ggaagatat 
      ggcgcgatt ttacattgc tgaaatggt tttagccat 
      agattcgat acataagct tggatatgt acacagaga 
      cattaaacc cgacaacgt gttattgga cgcaaatgg 
      ccatattag actagcaga ttttggtag ttgtttgaa 
      attaaatga cgatggaac agtgcagtc caatgttgc 
      tgttggaac ccctgatta tatctcccc agagatact 
      cagagcaat ggaggacgg tcaaggcaa atacggtcc 
      agagtgtga ctggtggtc tctaggtgt ttgcatgta 
      tgaaatgtt gtttggtga aacaccgtt ttacgccga 
      aagcttggt cgaaactta cgggaagat aatgaacca 
      taaaaactg ttttgattt tgcgactga tgttgatgt 
      gtctgatgc ggcgaaaga tttaatcaa gcagttaat 
      ttgtagtca agaatttcg tttaggaca gaatggaat 
      acaggattt taagaacca tccatggtt tgagggtgt 
      cgactggga tggtattag agatagtaa cgcgcctta 
      tattcccga agtttcgag tccaacaga tacatcaaa 
      ttttgacgt agacgatgc tgatatccg actgtcgga 
      tgccatgcc gccaactgc caataatgc ctttaccgg 
      acttcattt gccttttgt aggctttac tttcactca 
      aggaagttg tatatgcga cttgagtaa cctttatac 
      gaatataac gaacaatct agcggacaa gcaaagtaa 
      acgaaataa tggcgaaat tgtgagttt gaatttgaa 
      cgaagaaaa ggaaatgga taagagaat gtcgcctga 
      cggcactag gaaattgca agatgaaat taacacttt 
      aactaaacg taactgcga gctggagtc gcaattgcg 
      aagcttcga aagcggtca ttcgaagga tttgttcat 
      tgatacttt agatggtgc tgaaaatgc aaaagtgaa 
      agaattgga aaaactgaa ccgtatttt aaaacaaga 
      gaaggaaga agcaatgaa ggagaagtt agaccttca 
      tgaaaaact caaatttca agacaagga attgaaaga 
      tgcgttggc acaaaagaa actggccat ggccgagta 
      tacggaggt ttccgataa gttgtccga actgcgaca 
      acagaagca gaaatttag cagacaggt tagggacaa 
      ggaggagga attggaagc cgttatgca gaaagttga 
      ttcgctcag aaacgacat aagacgggc ggagaaatt 
      gagaaggga gttggaatc tcgcgttga tgaagctct 
      agctgaagc aacgaagga aaggaagtt gagggaacg 
      ctcggagga gtactgtcg gcaaatgca ggcagagtc 
      ggatcggtt gagagtgag gagtgagct agggcccag 
      agaccaaca agataattt aaggctaaa agcagaact 
      ggaaaaatt ggaggttca gtacaacga aagtttggc 
      gcaacaaca ggccaggtt caatctgga attatcctc 
      acttaggga acaactcca cgaagccga gtcccatcg 
      cgaactcct cgaacgcga agtgactga acttaaaga 
      aaaacaaga gaaacaccg tgtagaggc gctttccga 
      ttctgaaca aatgattgc cgaattgaa tcgtcgaag 
      cgaacgcga caaacaact tttgttgga cgataatca 
      taaacttac cgcaaatgt tgagtttct gactgaatc 
      tgtggagcg cttgcaatc ggaacgcgc gactttaga 
      agcggaata cgaacagtt gaggaacaa acaagaagc 
      gttgggaca atgggagtc gcaacttgc cgagattat 
      acagtgggt tagtgatga aaaggatgc tagggctta 
      tttgcaagc gctggctac gaaaatgac tgaagaatt 
      ggattattt gaaacatgc tggtgtttc aaatgcgat 
      gggtggcac tgataagaa ttggaggaa ccgaaggtc 
      gcaaaaact ggacaagat ggagctcct caaccttca 
      gagctcact acagtcgga aattcaagc caagcaagc 
      tataagcga ggagttgag caaaacaag agaggcctt 
      attagaagc gcaaaaaga tctgagaga ttcgaggca 
      gcgaaacga ggcctttgc cttggatct caaaaggaa 
      ggagaaaca aatcaagga gttgcaaag tcgtcttga 
      ttcggaagg atttttgga acgcccttc gtcccagat 
      gtcttactt ggaacaatt cctcaagga aaacaatca 
      gttgcaagt ccaaagtca aacttacga caacgtgcc 
      cccactttt gtaccaagg aggctcgat agagtctga 
      ggaaggcga tatcgaaga caaccgagc tttaagttt 
      gacgagttc taagagtaa cttgtctga actcagcat 
      gcacgattc ccagtctcc aatgaaaca caaaaacca 
      tcaattttt ggtgcgaac tttttctag tcctacaaa 
      atgcaatca ctgcacgtc attgatggt cggtttgat 
      gcgacaagg aatggtgtg cgagatttg cgggtttac 
      ctgtcacac gtcgtgttg tccacatgt gccggcaat 
      ttgtccgtt accgtcaga tcagaccaa gagaccgct 
      ggggattga ccccactag aggcatagg aactgctta 
      cgagggcta cgtcaaagt gcccaagca aggaggtgt 
      caaaaaggg ttggatcag acaatttgt tgttgtttg 
      tgacttcaa actgttttt atacgacat atcagcaga 
      tcgaaatgc cttaccaag tgttcacgt agctcaagt 
      tttagatat gagggatcc actgtttag tgtttctag 
      cgttaagga ttctgacgt catccacgc caacaaaaa 
      ggacattcc ttgcatctt caagatcac cacctcctt 
      gatggagcc cccgggccc gcgcaactc caccctgat 
      gttggccga ctccgaagc cgaaaagaa caaatgggt 
      ggtcgccct ctccgaact ccatcgaat aatgaagcg 
      aaacaattt acccaacac tacggtgtt actagcgcg 
      cgaattgat cgataactc cctaacttt gctaagaaa 
      cacactaag tgctgcgat tatcgaccc agatcgcat 
      agtcctagg cgccgaaga aggacttca ctgtgtcga 
      tctagaccg cagcgagat tgcgaaaat cggcgaagg 
      caaaaagat cttccaact ggagtatat cagtgagga 
      gcacctgtt ggtcgtggt cagtggtaa acagagata 
      cgtacggtt ggtccccat cagagcact ggacggcga 
      tgagactga atggatcaa agtggcgga gagtaaagg 
      gtgttcggt gctgacggt ggggcctgt tgtgagggc 
      gcccttaac gttctgtct atgtgtggc gattaaaaa 
      acagcaaaa ttcctcgac tgttatcat atacgagat 
      aacgaggac acggacgcg acaccatcg aattcggga 
      cataaatct gggggcgcc agttcagtc cttgcagtt 
      gttgtcaga tggccgact ttgtatcgg ttctccgtc 
      gaattttac gatttgtaa tctacaaac tgaacaacc 
      acttttttc tttggtgag ttcggagaa tccactaca 
      tgccacgct ggcttgtaa tgcagttga ggcactaag 
      agtgataga gttgcctcg gcatgaata ccttctagt 
      ttttggtac tttggcggc ttatgttga tagacacgg 
      ccgcaaatc tcgtgatag ggaaataat gtatccagc 
      agtgccgac agccatatg ctatagaga tggtcatct 
      catcgttta tagtgagac ccatatcga tatttttaa 
      ctgtgcaac gggagaatg ggttcaaac tcttaatat 
      caagaaagc aaagcctct taatgatag tggggcact 
      ttcgttgtg cattctaaa tgatttaac tcatctgct 
      gtatctgtc taatataca tcagcgtga attaattaa 
      tttagataa tgtggttac gttagatag agacggacg 
      gactttgca gagaccgcg gaggcggtt ttcgctgag 
      agaggggaa caggtcaca acgaataaa cacggaccg 
      ccggtcaaa actgatttc ggcgccgac aaacttcaa 
      ccacataag tcacatggg ccccggcga gggcataca 
      gagacaaag actgattga tctttcgac gaacttagt 
      tgatactcc tgatcagtc ctcctacgg tcctcaggt 
      gcaaccaag acagccaac caagatggc gcccctgcc 
      cccgaaaca cggcgaacg caagcgtct agaaatatc 
      aaaccccat tcgggggcc ccctttccc gcccgcgta 
      caacggggc caagcgggc tgcgccccc gcgcccgcg 
      agatcttcc accggcctt gccgcgccc ttccgacac 
      gtcaccttc cccagatcc tgcgagcat tggaagcat 
      gtcgtcgct gcacgatat gatcaaggg cacggagaa 
      aggcagtcc tcgccactc gatcgcgag caataacag 
      ttcaaacct ttcgactcc gccgtcgcc ccaagcaga 
      tcatcataa tagttcgag ttacgatag ctaaacgtc 
      gcattcaag acttataat agttgaata tgattagac 
      ctaacatgt atgtgcgaa aatgtctga ccttatgcg 
      aagtgttca cgcactttg tatatttga acttttagt 
      tacctcaac aaaaacgaa taaaatttt aaaccggta 
      tgacttcag ttgattgta tttagttta actagttaa 
      ggtggcact gtacttatc tcttctttt gtaaatttt 
      gtgtatttt gtgttgttt ataaaatgc tacacttgt 
      aaaataatt cgagtcaat taattaact gttgtaaga 
      tttatttta tgtactgca aattggatg ttattattt 
      atatcaggc gctattact gcagcagag tgtaatact 
      gccaaaatt ttaattgag aatatttat atttattta 
      ttgcttttt gttttttaa ttttttatt gaaattatt 
      ttgtactag tgatattta ttagtaagg tatttcgat 
      attcatata gataaatat ttattactg tatatgcaa 
      ggtttagta caattttat attagtttt caacacacg 
      gcaagacaa tatgggtgt tttatcagg tggtaaagc 
      caattttaa tatttacat tattagatt ttctactgt 
      gacttcagg tttaagtat ttatcggca tattagcgt 
      tcattagtt catattcac gttaggaaa gacattgta 
      ttggtgcac taaatctgc cttttcgtt tcgcggtag 
      aaagaactt gtttccata ataatatag caaaggcat 
      tatgtaaca tactagata acatattta ttttattaa 
      tagagttat ttatattaa caatgcgga ggatatagt 
      attataaca ctaaaacca gccttttat t
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