Status
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Gene information
General information
TC008350 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8920360..8924396
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008350
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09943

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008350 RA
      MFSQGLASA QKFALYLYN NNHLDKNFS IEKEHAKKL 
      YNEKFKGEN KIKFGTIFH FTVNMLVWQ KNLDVMFYN 
      KNKLLKLLN KEAQIDTRL KSNNVKKKT VVSQNLSFN 
      KQQPEEILL SPQECKLCN VRFDKQNKL EDHVNKKEH 
      TFSDSLKKA LKERKEVLL EFAVDGSPI LNNLIIRKN 
      VPKTFVLTI NNPSKNSVK ITKISTKDN VEQVNFNHS 
      CPYDLPPLQ TIQLELLIR NTTVTNMLY FPILLTVET 
      VRGIHQYAI ELVFHTHDE FFDDLHPVE PFKRATKPV 
      FEEFVQEID KGERPNIFP QRKLKVSLN NYRLSFDII 
      ACLNAGLKE FPGMSSAQR GQLLYLKSL LNVDNKQNC 
      LTPRNYTKM LDMLLYFEE HQMLKNIKD YDREDQILK 
      KAGGGLFEL EVPGLAEHR PSVVFGDAV YIRENKKST 
      TLFEGFVHK VKESSVLLK FSHRFATDI FVNGKKFYV 
      SFTFNRYPI RVEHQAVDF CRIDNVAHL LFPSKIPNS 
      PISSAPLVW FNRDIEGNP HQQQAVHAV LQKTAMPAP 
      YLIFGPPGT GKTMTVVES VKQIYKKGN EKILICTPS 
      NNAANEVTK RLINTIPAT DLFRYIAPS FPNHLIPKE 
      IKKYINFPG GNYTLPTIE QFTKYRIVI TTLTSAAKL 
      VNGGCPGDH FSYVFIDES GQATETETL VAIAGILTS 
      QAKQGVFSG QLVLAGDPR QLGPVIHST LAKEYGFGT 
      SMLERLMDT CVCYQKNAN SKKYNPSAL TKLVKNYRS 
      HKVIIDQPN KLFYDDELE SAGDNLINA ALNWEHLPN 
      RQFPLIFHS VVGIDQREK TSPSFFNIQ EVQIVMDYL 
      NKLIGCKMQ GISINEDHI GVVTPYKKQ AEKIRHSCL 
      KKKYSESLM VGTVEQFQG QEKLIIIIS TVRSKSDLI 
      KFDLKFHLG FLNNPKRFN VAITRAKAL LIIVGNPNI 
      LQHDNYWRS MISYCENNN ALIGDKFVF DNSGDTKMD 
      DLIRQAQVM TITDGPSLE ERPNFSRGN V
    CDS sequence
    • >TC008350 RA
      atgttttcc caaggttta gcttccgcc caaaaattt 
      gctttgtac ctttacaac aacaatcac cttgataag 
      aatttttct attgagaag gaacacgct aaaaagctt 
      tacaatgag aaattcaaa ggtgaaaat aaaataaaa 
      tttggcacc atatttcat ttcaccgtc aatatgctt 
      gtttggcaa aaaaatctc gatgttatg ttttataac 
      aaaaataaa ttgctaaaa ttgttaaac aaagaagca 
      caaattgac acacgtctg aaaagtaat aatgttaag 
      aaaaaaaca gttgttagt caaaatctt tctttcaac 
      aaacaacaa cctgaagaa attctgttg tctccacaa 
      gaatgcaaa ctgtgtaat gtcagattt gataaacag 
      aacaaactt gaggaccac gttaataag aaggaacac 
      accttttcc gattcgctc aaaaaggct ctaaaagaa 
      cgtaaagaa gtactgctt gagtttgcc gttgacggt 
      tccccaatc ttgaacaac ttaatcatc cgtaaaaat 
      gtcccaaaa acgttcgtc ttaacaata aacaaccca 
      agcaaaaac tccgtaaaa attacaaag attagcacc 
      aaagacaac gtggaacaa gtgaatttt aaccactcg 
      tgcccttac gacctacca cctctccaa acgatacaa 
      ctggaatta ttaattcgt aatacaaca gttacgaat 
      atgttgtat tttcctatt ttactcact gtcgagact 
      gtcagagga attcaccag tacgctatc gaattggtc 
      tttcataca cacgacgaa tttttcgac gacttgcat 
      ccagttgaa cctttcaag cgcgccact aaacccgtt 
      ttcgaggag tttgtccag gaaatagac aaaggtgaa 
      cgaccaaat attttcccg cagagaaaa ctcaaagtg 
      tccttaaat aattatcgt ttgtctttt gatattatc 
      gcttgttta aatgcgggg ttgaaagaa ttccccgga 
      atgtcttct gcacagcgc ggacagtta ctttatttg 
      aagagtttg ctcaatgtt gataacaaa caaaattgc 
      ttgacaccg cgaaattac accaaaatg ttagacatg 
      cttctctat tttgaagaa caccaaatg ttgaaaaac 
      atcaaggat tacgaccga gaggaccag attttgaag 
      aaagctggg ggcggcttg tttgaactt gaggtcccc 
      ggtcttgcg gagcatcgc ccgtctgtt gttttcggc 
      gacgctgtt tatatcaga gagaacaag aaaagtaca 
      acactgttt gaaggattc gtgcacaaa gttaaagaa 
      tcaagcgtt ttattaaaa ttttcccac cgttttgct 
      accgacatt ttcgtaaac gggaaaaaa ttctacgtc 
      agttttacc ttcaaccgt tacccgata agggtcgag 
      catcaggct gtcgatttt tgccgcatt gataacgtc 
      gcccatttg ctgtttccc tccaaaatc cccaactcg 
      cccatttct agcgctcct cttgtttgg tttaatcgg 
      gacattgag ggaaacccc caccaacag caagcagtg 
      cacgctgtt ttacaaaaa acagcaatg cccgcccct 
      tacttaatt ttcggaccg cctggaacc ggtaaaacc 
      atgacggtg gtggaatcg gtgaaacaa atttataaa 
      aaaggcaac gagaaaatt ttgatttgc actccttca 
      aataacgca gcgaatgaa gtgaccaaa cgtttgatt 
      aacacaatt ccggccact gatctcttc agatacatt 
      gcccccagc tttcctaac cacctaata cccaaagaa 
      atcaaaaag tatatcaat tttccggga ggtaactac 
      accttgccc acgatcgaa caatttaca aagtatcga 
      attgtgatc acaacactg acttcagct gcgaaatta 
      gtcaacggg ggttgtccg ggggaccac ttcagttat 
      gtgtttatt gacgaaagt gggcaagct accgaaact 
      gaaactttg gtggctata gctggaatt ttgacgagt 
      caggcgaaa cagggagtt ttttcgggg caacttgtt 
      ttggcgggg gatcccaga caacttggg ccagtgatt 
      cactcaact ttagccaaa gaatatggt ttcggcact 
      tccatgttg gaacgtttg atggacacc tgtgtttgt 
      taccaaaaa aatgctaac tcaaagaag tacaatccg 
      agcgctttg accaaactc gtgaagaac tatcggtcc 
      cataaagtc attatagac caaccgaat aagttgttt 
      tatgacgat gaattagaa tcggcgggt gataatttg 
      ataaatgcg gccttgaac tgggagcat ttgcccaat 
      cgtcaattc ccgttgatt tttcacagc gtcgtggga 
      atagaccaa cgtgagaaa acatcaccc agttttttc 
      aacattcaa gaagttcaa atcgttatg gattacttg 
      aataagttg atcggttgt aaaatgcaa ggtatttcg 
      atcaacgaa gaccatatt ggggtcgta acaccgtac 
      aaaaaacaa gccgagaaa ataagacac tcctgtttg 
      aagaaaaaa tatagcgaa agtttaatg gtggggact 
      gttgaacag tttcagggt caagaaaaa ctgatcatt 
      attatatca actgtcaga tccaagtcg gacctgatc 
      aagtttgat cttaaattc cacttaggg tttttgaac 
      aatccaaag cgtttcaac gttgccata actcgggcc 
      aaagctttg ttaattatc gtcggaaac cccaatatt 
      ttgcaacac gacaattac tggaggagc atgatttcg 
      tactgcgag aacaataat gctttaatt ggcgataaa 
      tttgttttt gataactcg ggtgacaca aaaatggat 
      gacttaatt cgtcaagca caagtcatg acaattacc 
      gatgggcca agtcttgaa gaacgtcca aacttttcc 
      agaggcaat gtttaa
    mRNA sequence
    • >TC008350 RA
      ttcaaatta aaatgtaag caaacgtcc gtaaattgc 
      taatattgg cggcaaatt tcaaattac cgaatcgga 
      aatcatcag tctaatcag gacccccac tacgcacgc 
      attaaattt gctgaggca agaaacctt actgttctg 
      tggtcctat ctgtggatt ttatttatc tcagtttag 
      cgactttaa gtgtaattc agtgataaa gatggttta 
      aaaaaatta aattggtgt ttcgggaag ctcaatatt 
      aggggcttc acactcgac caaaaaatt gttaggcaa 
      gcgctccaa aaagatgtt ttcccaagg tttagcttc 
      cgcccaaaa atttgcttt gtaccttta caacaacaa 
      tcaccttga taagaattt ttctattga gaaggaaca 
      cgctaaaaa gctttacaa tgagaaatt caaaggtga 
      aaataaaat aaaatttgg caccatatt tcatttcac 
      cgtcaatat gcttgtttg gcaaaaaaa tctcgatgt 
      tatgtttta taacaaaaa taaattgct aaaattgtt 
      aaacaaaga agcacaaat tgacacacg tctgaaaag 
      taataatgt taagaaaaa aacagttgt tagtcaaaa 
      tctttcttt caacaaaca acaacctga agaaattct 
      gttgtctcc acaagaatg caaactgtg taatgtcag 
      atttgataa acagaacaa acttgagga ccacgttaa 
      taagaagga acacacctt ttccgattc gctcaaaaa 
      ggctctaaa agaacgtaa agaagtact gcttgagtt 
      tgccgttga cggttcccc aatcttgaa caacttaat 
      catccgtaa aaatgtccc aaaaacgtt cgtcttaac 
      aataaacaa cccaagcaa aaactccgt aaaaattac 
      aaagattag caccaaaga caacgtgga acaagtgaa 
      ttttaacca ctcgtgccc ttacgacct accacctct 
      ccaaacgat acaactgga attattaat tcgtaatac 
      aacagttac gaatatgtt gtattttcc tattttact 
      cactgtcga gactgtcag aggaattca ccagtacgc 
      tatcgaatt ggtctttca tacacacga cgaattttt 
      cgacgactt gcatccagt tgaaccttt caagcgcgc 
      cactaaacc cgttttcga ggagtttgt ccaggaaat 
      agacaaagg tgaacgacc aaatatttt cccgcagag 
      aaaactcaa agtgtcctt aaataatta tcgtttgtc 
      ttttgatat tatcgcttg tttaaatgc ggggttgaa 
      agaattccc cggaatgtc ttctgcaca gcgcggaca 
      gttacttta tttgaagag tttgctcaa tgttgataa 
      caaacaaaa ttgcttgac accgcgaaa ttacaccaa 
      aatgttaga catgcttct ctattttga agaacacca 
      aatgttgaa aaacatcaa ggattacga ccgagagga 
      ccagatttt gaagaaagc tgggggcgg cttgtttga 
      acttgaggt ccccggtct tgcggagca tcgcccgtc 
      tgttgtttt cggcgacgc tgtttatat cagagagaa 
      caagaaaag tacaacact gtttgaagg attcgtgca 
      caaagttaa agaatcaag cgttttatt aaaattttc 
      ccaccgttt tgctaccga cattttcgt aaacgggaa 
      aaaattcta cgtcagttt taccttcaa ccgttaccc 
      gataagggt cgagcatca ggctgtcga tttttgccg 
      cattgataa cgtcgccca tttgctgtt tccctccaa 
      aatccccaa ctcgcccat ttctagcgc tcctcttgt 
      ttggtttaa tcgggacat tgagggaaa cccccacca 
      acagcaagc agtgcacgc tgttttaca aaaaacagc 
      aatgcccgc cccttactt aattttcgg accgcctgg 
      aaccggtaa aaccatgac ggtggtgga atcggtgaa 
      acaaattta taaaaaagg caacgagaa aattttgat 
      ttgcactcc ttcaaataa cgcagcgaa tgaagtgac 
      caaacgttt gattaacac aattccggc cactgatct 
      cttcagata cattgcccc cagctttcc taaccacct 
      aatacccaa agaaatcaa aaagtatat caattttcc 
      gggaggtaa ctacacctt gcccacgat cgaacaatt 
      tacaaagta tcgaattgt gatcacaac actgacttc 
      agctgcgaa attagtcaa cgggggttg tccggggga 
      ccacttcag ttatgtgtt tattgacga aagtgggca 
      agctaccga aactgaaac tttggtggc tatagctgg 
      aattttgac gagtcaggc gaaacaggg agttttttc 
      ggggcaact tgttttggc gggggatcc cagacaact 
      tgggccagt gattcactc aactttagc caaagaata 
      tggtttcgg cacttccat gttggaacg tttgatgga 
      cacctgtgt ttgttacca aaaaaatgc taactcaaa 
      gaagtacaa tccgagcgc tttgaccaa actcgtgaa 
      gaactatcg gtcccataa agtcattat agaccaacc 
      gaataagtt gttttatga cgatgaatt agaatcggc 
      gggtgataa tttgataaa tgcggcctt gaactggga 
      gcatttgcc caatcgtca attcccgtt gatttttca 
      cagcgtcgt gggaataga ccaacgtga gaaaacatc 
      acccagttt tttcaacat tcaagaagt tcaaatcgt 
      tatggatta cttgaataa gttgatcgg ttgtaaaat 
      gcaaggtat ttcgatcaa cgaagacca tattggggt 
      cgtaacacc gtacaaaaa acaagccga gaaaataag 
      acactcctg tttgaagaa aaaatatag cgaaagttt 
      aatggtggg gactgttga acagtttca gggtcaaga 
      aaaactgat cattattat atcaactgt cagatccaa 
      gtcggacct gatcaagtt tgatcttaa attccactt 
      agggttttt gaacaatcc aaagcgttt caacgttgc 
      cataactcg ggccaaagc tttgttaat tatcgtcgg 
      aaaccccaa tattttgca acacgacaa ttactggag 
      gagcatgat ttcgtactg cgagaacaa taatgcttt 
      aattggcga taaatttgt ttttgataa ctcgggtga 
      cacaaaaat ggatgactt aattcgtca agcacaagt 
      catgacaat taccgatgg gccaagtct tgaagaacg 
      tccaaactt ttccagagg caatgttta atattcctg 
      atttttgtt attttgagg catgaatta taattttta 
      tttgctggt cattaaata taaaaatgt gggctaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY