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Gene information
General information
TC007157 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8386043..8394100
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007157
    iBeetle screen iB_09936

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007157 RA
      MDGDLIEKL LQTSGLSCE DLLVKLQQY KLKQSSKSQ 
      ENKQDENLV YGENGREQQ CTTNNDNSV ILETQSSTV 
      DSSNGANDI TPEEHTTEV ADKPVEEKS ETDHKDENS 
      CDSSFIQKV ESSNEIPEK PYFEDTDSC KAVILEDAK 
      KKEGSEEIL TQSLEEKHE ASDQNLKEV ENSEAVVEV 
      ETFDKNEAS CSKNSSEEI EDKQVEETI RENIVERLL 
      RKTSATHHK DTEKSDLLV QDSKDSCEG IGVNKDSKG 
      DSGVNENAI KEENKMFSE DSSNSADIH NYSDLVNSL 
      EEDSASCAS GLSKSTVDT SESIPMAQS QMAISTVEE 
      LPRSEEQLR DVIKMQLSS LRNMKKSSG YLPDLNRKS 
      IFFHNLRKK RKSCDVKTI KPDTERENN EEEGKILEE 
      NCKETVTND DSLVSLSDQ TDFLILDVT GGIDRLTPE 
      INENDNEKT EERCFAAVS SSGVEDVPI ESNTELPTV 
      NNIEDATKN YSPSKEIRD LLTSYRKKM SLNYFDANF 
      STLKGHDKF KKTQDQHQN KSSEDDSPA PETPSKVVL 
      ISVKKNISE NFNEDPGIK RSVKNNEKS PFVSDSLED 
      FLKNESAQL KISYPIPKL GAEEGINES TKEISPFDS 
      MVKVQKIEP VIEKETPQR ERKLIKAKT LADMRRQFE 
      KLNAKEAKK REKKEIKSE GPYVPFHRN SHIIYNNKK 
      LWVTTKTST SCAGVIGSS NSEQFSPPS NKKLSLLST 
      LHQRPKRSV SYKPGPLSK KCKLQDAFS RDKWTTELK 
      ALPQIYLEV TPQFNKPID ADVVHLVPI FDDCVITEE 
      RTNFALTAL KTDSEPKSY TFPIPYKNN QTHLIVRKR 
      VEPVAATTP VAEADPEKI VASVLNDLI SYVEVKEIE 
      DSLIKEDDV VLEEPEPVP DGTPKSRGR GRRLKRNKV 
      DMELLRLSC KVIDVATNG TEDKPCSKS YCKMGCVCR 
      SLLCESVFS DHCQMVDCM FGCKCPPSD TSSNSLHVH 
      TVNHLEDKA KKNLAKLER EFTQTVIHT DNDTILVGS 
      GKVRRCTKT PKKYTDYIG DVDDVFVKP EPDVEYKRP 
      KVKKCSVVL ERLNLRNIK PLCLFHRMY ECGRCVNRL 
      PLVDKPYCF VHNVFNCAC NKDTNVRKT NYEVVKYKT 
      GESCARIRK VFSQYKERN KDPIYNAKL RKKNPYSFL 
      PQVMKETEV ASKPTLIQF QSKQGSKRK QSFVVREEE 
      PVDPGKETV LSYRDNQTD EDREIQVVS PNKEFHFVS 
      PDDLQAVSP DKEFVLQRR RKKKLVMET QFQKANNGI 
      FDGEIKLSL KENQCLIPF VHNQPSLEY LRILPWTNL 
      IKNYNTRKM SIWAKESGT TILCNAAGY LPPRNFVNI 
      RRLKHKSEF INWIVSNTL PDDIPRHLM HVILSPRKN 
      HYMISGYCL KKAEDLSAR HGARETQAS TSFENLLTN 
      VDSYETNCG KTDDLSLNS LLKNHSTFK FSRLYKSVL 
      LKPKSKPVV KLKTATTSI KPVSGGAME VNVLPLPRR 
      PSHRDTETI EIDCGSTSQ DGVSRPTSD VYDLDEVTS 
      RLPKKLSDD TNSSALVPE RTLKCTHKD MHNHKKTLV 
      LTIKEVPKR RIYFDKDSP GDDFIVPLP RVHKFCRWR 
      MIYLNSDFS YLNFTKSNY SIKYTDLLD VVKMASTVR 
      STITLRNQE IRQFYKTYT FGIFCVPDL QDRIFVGPY 
      LKTENHNVE TLRYLQGKL IDTETFNKI IGKKFNERS 
      PVWLYETLP HNSIPDGLV IDITEDDEV PNDVSSEPS 
      SRNLLCPPF VTALSDEEL KAKHSINRY IVTNIPYLG 
      YFGACQRDS KEIEVFWPN NTRGYRFPN VTLAKNLLI 
      HYLSQIFCP IPQTFKINV IIMTQLDLK TNTPVKARY 
      LTEPGPFFC GEFGIYEKE SVPEDLLQE CNVPSLDSL 
      LQTYKESLE NDESIVKLF GLFLMSNEE LRKFPEPQQ 
      IIQWARTEI YIQEKLSDN YESEKSDLK SMISSKMQR 
      IKLLRRGIR DDQMSSKVS ETIEIPDSD DEDRHVSKV 
      FVMQSAQKG KSFVLKQNI SVLKEKDAK QSPVKNLGM 
      PVVNKVI
    CDS sequence
    • >TC007157 RA
      atggacggt gaccttata gaaaagctt ttgcaaacg 
      tcgggttta tcatgtgaa gatttgctt gttaaactt 
      cagcaatac aaattaaag caatcgagc aaatcacag 
      gaaaataaa caggacgaa aacttagtt tatggagaa 
      aacggccgt gaacagcag tgcactaca aataacgat 
      aattctgtg atattagaa acccaaagt tctacagta 
      gatagtagt aacggtgct aatgacatt acacctgaa 
      gaacacaca acagaagtc gcagacaaa ccagttgaa 
      gaaaaatct gaaactgat cataaagac gaaaacagt 
      tgtgatagt tcttttatt cagaaagta gaatctagt 
      aacgaaatt ccggaaaag ccatatttt gaagacact 
      gatagttgc aaggctgta attttggaa gatgcaaaa 
      aagaaagaa gggagcgaa gaaattctt acacaatca 
      cttgaagaa aaacatgaa gcaagcgat cagaattta 
      aaagaagta gaaaatagt gaagcagtt gttgaagta 
      gagacattt gataagaat gaagcgagt tgcagcaag 
      aatagtagt gaagaaatt gaggataag caagttgaa 
      gaaacaatt agagaaaac attgttgaa cgactactt 
      cgaaaaaca tcagcgaca catcataaa gacactgag 
      aaaagcgat ttacttgtg caagactca aaagatagt 
      tgtgaagga attggggtc aacaaggat tcaaaagga 
      gatagtgga gttaacgaa aatgcaatt aaagaagag 
      aataaaatg ttcagtgaa gactcgtcc aacagtgct 
      gacatacat aattacagt gatcttgta aacagttta 
      gaagaagat tcagcttct tgtgcaagt ggactatcc 
      aaatcaacc gtcgatact tccgaaagt attcccatg 
      gctcagtca caaatggct atctctacc gttgaggaa 
      ctcccaaga tctgaagaa cagttaaga gatgtaata 
      aaaatgcag ctctcttcg ttacgtaac atgaaaaaa 
      agtagtggc tacctccca gatcttaac aggaaatcg 
      atatttttt cacaatctg agaaagaaa cgaaaatcg 
      tgcgacgta aaaacgatt aaacctgac acagaacgt 
      gaaaataat gaagaagag ggaaagata cttgaagaa 
      aattgcaag gaaactgta actaatgat gattcttta 
      gtgtccttg tcagatcaa acagatttt cttattctt 
      gatgtcact ggaggaatt gataggtta actccggag 
      ataaatgaa aatgataac gaaaaaact gaagagcga 
      tgctttgct gctgttagt agtagtggt gttgaggac 
      gtgccaatt gaaagtaat actgagtta cctactgta 
      aataatatc gaagatgct acgaagaat tactcacct 
      agtaaagaa attagagac cttcttaca agttatcgg 
      aaaaaaatg tctttaaat tacttcgac gctaatttt 
      tctacactg aaaggtcac gacaagttc aaaaaaacg 
      caagatcaa caccaaaac aagtcaagt gaagatgat 
      agtccagct cctgaaact ccaagcaaa gtagtgctc 
      atttcagtg aagaaaaac atttctgaa aacttcaat 
      gaggatcct ggaataaaa agatccgtc aaaaataac 
      gaaaaatcc ccgtttgta agcgactct ttggaagat 
      ttcctaaaa aatgaaagt gctcagttg aaaattagt 
      taccctatc ccgaaatta ggagccgaa gaaggaatc 
      aacgaaagt accaaagag atcagccct tttgacagc 
      atggtcaaa gtgcaaaaa atagaaccg gtcatagag 
      aaggaaact ccccaaaga gagcgtaaa ctaatcaag 
      gcaaaaact ttagctgac atgaggaga caatttgaa 
      aaattaaac gcgaaggaa gcgaaaaaa cgagaaaaa 
      aaggaaata aagagtgag ggcccatat gttccattt 
      caccgcaat agtcatatc atttacaat aacaagaaa 
      ttgtgggtg actactaaa actagcact agttgcgcg 
      ggtgttatc ggctcatca aacagtgaa cagttctca 
      cccccaagt aacaaaaaa ctgtcactt ctctcaaca 
      ttacaccaa cgaccgaag aggagtgtt agttacaag 
      cctggacct ctcagcaag aaatgtaaa ctacaagat 
      gccttcagt cgggataag tggacaact gaactgaaa 
      gctttgccc cagatttac ttggaggtg acgccacag 
      ttcaacaaa ccgattgat gcggacgtg gttcacttg 
      gtgccaatt tttgatgat tgtgtcata accgaggaa 
      agaacaaat ttcgctttg acagctttg aagacggac 
      agcgaacca aagtcgtac actttcccc atcccatac 
      aaaaacaac caaacgcac ctaattgta cgaaagaga 
      gtagaacca gttgcggct actacaccg gttgctgaa 
      gcagatcca gaaaaaatt gttgcaagt gtgcttaac 
      gaccttatc agttatgtc gaagtcaag gaaattgaa 
      gacagttta ataaaggaa gacgatgtt gttttggaa 
      gaaccagag ccggttcct gacggaact cccaaatct 
      agaggtaga gggcgcagg cttaagcgg aataaagtc 
      gacatggaa ctacttcga cttagttgt aaagtcatc 
      gatgtcgcg acgaacgga accgaagac aagccttgt 
      tccaaatct tactgtaaa atggggtgt gtgtgcagg 
      agtttactt tgtgaaagc gtcttttcc gatcattgc 
      caaatggtt gattgcatg tttggatgc aagtgtcct 
      ccgagcgat acttcaagt aactcactg catgttcac 
      accgtcaac catttggaa gataaagct aagaagaac 
      ttggcaaaa ctggaacga gaatttacc caaactgta 
      atccacact gacaatgac acgattctt gttggatca 
      ggcaaagtg agaagatgc acaaaaaca ccaaagaaa 
      tacaccgat tatattggc gacgtggac gatgttttt 
      gtaaaacct gaacccgac gttgaatac aagaggcca 
      aaagttaag aaatgttca gtggttttg gaacggctg 
      aatttgagg aacattaaa cctctttgc ctctttcat 
      agaatgtac gagtgtggt agatgtgtt aacaggtta 
      ccacttgtc gataaaccg tattgtttt gttcataat 
      gtattcaac tgtgcgtgt aacaaagac acaaatgtg 
      aggaaaact aattacgaa gtcgtcaaa tataagacg 
      ggtgaaagt tgtgcacga atccgtaaa gttttttca 
      cagtataaa gaacggaat aaagatcct atatacaat 
      gcaaagttg cgaaagaag aatccatat tctttcctt 
      ccgcaagtc atgaaagag actgaggtg gcaagtaaa 
      ccaacatta atacaattt caaagtaag caaggaagt 
      aagcgcaaa cagtctttt gttgttcgt gaggaggaa 
      ccagtcgat ccaggaaaa gaaacagtc ctgtcctat 
      cgagataat caaactgat gaagacagg gagatacaa 
      gttgtttct ccaaataaa gaatttcat ttcgtttct 
      ccagatgac cttcaagcc gtttccccg gataaagaa 
      tttgtcctt cagaggcgg aggaaaaag aagctagta 
      atggaaaca cagtttcag aaagcaaat aatggtatt 
      ttcgacgga gaaatcaaa ctttccctc aaagaaaac 
      caatgcttg atacctttt gtccacaac caaccttcc 
      ttagagtac ctccgaatt ttaccatgg acaaacttg 
      attaaaaac tacaacacc cgcaaaatg tccatttgg 
      gcgaaggaa agtggaaca acgattctc tgcaacgct 
      gccggttat ctcccaccg agaaacttc gtcaacatt 
      agaagatta aaacataaa tcggaattt atcaactgg 
      atcgtctca aacacctta ccggacgac atacctcgc 
      caccttatg cacgtcata ttatcgcca aggaagaac 
      cactacatg atttccggt tactgctta aagaaagct 
      gaagatttg tccgctaga catggagca agagagaca 
      caagcttct accagtttt gagaatctc ctaacaaat 
      gtggacagc tacgaaacc aattgtggc aaaacggac 
      gatcttagt ctaaactcg cttcttaaa aatcacagc 
      acgtttaag ttttcgcga ctgtataag tcggtgtta 
      ctgaaaccg aagtcgaaa cctgtggta aagctcaag 
      acagctact acgtccata aaaccagtg tcagggggc 
      gcaatggaa gtgaacgtg ctcccactt cccagacgc 
      ccctcacac cgggacact gagactatt gaaattgat 
      tgtggctct acttcgcaa gatggggtt tcaagacca 
      acgagtgat gtttacgat ttggacgaa gtcacttcg 
      cgactacca aaaaaattg tccgacgat acgaactca 
      agtgctcta gtgccggag agaactttg aaatgcaca 
      cacaaagac atgcacaac cataagaag actcttgtt 
      ttgaccatc aaagaggtg cccaaacgt cggatttac 
      ttcgataaa gacagtccg ggggatgat tttattgtg 
      ccactgcca agagttcat aagttttgt cggtggagg 
      atgatttat ctgaattcc gacttcagt tacctcaac 
      ttcaccaaa tcaaactac tccattaaa tacacagat 
      ttgttggat gttgtgaaa atggcaagt acggtgaga 
      tcaacgata acgcttcgc aatcaggag atacgacag 
      ttttataaa acttacacg tttgggatt ttttgcgtt 
      cctgacctc caagaccgc atctttgtg ggtccttac 
      ctcaaaaca gagaaccac aatgttgaa acgttgagg 
      tacttacaa gggaagttg atcgacact gagacgttc 
      aataagata attgggaag aaatttaac gaacgaagc 
      cctgtctgg ctttatgaa acgctacct cataacagc 
      atacccgac ggacttgtg atagacata actgaagac 
      gatgaggtc cctaatgac gtttcatca gaaccctca 
      tccaggaat ctgctttgt ccaccgttc gttacagca 
      ttaagtgac gaagagttg aaggcgaag cacagtatt 
      aacaggtac atcgttact aatatccct tacttggga 
      tatttcggt gcttgccag cgcgacagc aaagaaatt 
      gaggtcttt tggcccaac aatacaaga ggttaccgg 
      tttccaaac gtgaccctt gccaaaaat ttgttaata 
      cactacttg tcccaaatt ttctgcccc atcccccaa 
      acgttcaaa atcaatgtt ataataatg acgcagcta 
      gacctaaag acaaacacg ccggtcaaa gccagatat 
      ttgaccgaa cctggaccc tttttctgc ggcgagttt 
      ggtatctac gagaaggag agtgttccc gaagacctg 
      ctccaagag tgtaacgtg ccgtcgctc gactcactt 
      ttacaaacg tataaagaa tctctagaa aatgacgaa 
      tcaattgtc aagttgttc ggtttgttc ctaatgtcg 
      aacgaggag ttgcggaag ttccctgag ccccaacaa 
      atcatacaa tgggcgcgg actgaaatt tacatccag 
      gagaaactc tccgataat tacgaatcg gaaaaaagc 
      gatttgaaa tcgatgatt agcagcaaa atgcaacga 
      ataaaactg ttgcggaga ggaataaga gacgatcaa 
      atgagcagt aaagtgtcg gaaactatc gagattccg 
      gattccgat gatgaagat cgccacgtg tcgaaagta 
      ttcgtaatg caatcggca caaaaagga aaaagtttt 
      gtgctgaaa cagaatata tcagtactc aaagagaaa 
      gacgccaaa caatcgcca gtgaaaaat ctcggaatg 
      ccagttgta aataaagtt atttga
    mRNA sequence
    • >TC007157 RA
      gcaaagata cggttacgt ctcggcatt tcttcaaat 
      gcgcgggac aggaaacta ggattacgg aacgcgtgc 
      ttccgcttc cacatgtga tataaccgc cattttgtt 
      gagcttacg tgattaaaa aatatcatc caggggtaa 
      aattcggtt attatttga tttttcttg ggtaatgtt 
      ggtaaatta gtgtttata tttggctta cgtgacgtg 
      ggtaacgaa tgcaaacat ttcgagtcg ggaaaatgg 
      cgacgtaag gtttcgtcc attttagca gccatcatc 
      aggttaaca caaaatcaa aaatggacg gtgacctta 
      tagaaaagc ttttgcaaa cgtcgggtt tatcatgtg 
      aagatttgc ttgttaaac ttcagcaat acaaattaa 
      agcaatcga gcaaatcac aggaaaata aacaggacg 
      aaaacttag tttatggag aaaacggcc gtgaacagc 
      agtgcacta caaataacg ataattctg tgatattag 
      aaacccaaa gttctacag tagatagta gtaacggtg 
      ctaatgaca ttacacctg aagaacaca caacagaag 
      tcgcagaca aaccagttg aagaaaaat ctgaaactg 
      atcataaag acgaaaaca gttgtgata gttctttta 
      ttcagaaag tagaatcta gtaacgaaa ttccggaaa 
      agccatatt ttgaagaca ctgatagtt gcaaggctg 
      taattttgg aagatgcaa aaaagaaag aagggagcg 
      aagaaattc ttacacaat cacttgaag aaaaacatg 
      aagcaagcg atcagaatt taaaagaag tagaaaata 
      gtgaagcag ttgttgaag tagagacat ttgataaga 
      atgaagcga gttgcagca agaatagta gtgaagaaa 
      ttgaggata agcaagttg aagaaacaa ttagagaaa 
      acattgttg aacgactac ttcgaaaaa catcagcga 
      cacatcata aagacactg agaaaagcg atttacttg 
      tgcaagact caaaagata gttgtgaag gaattgggg 
      tcaacaagg attcaaaag gagatagtg gagttaacg 
      aaaatgcaa ttaaagaag agaataaaa tgttcagtg 
      aagactcgt ccaacagtg ctgacatac ataattaca 
      gtgatcttg taaacagtt tagaagaag attcagctt 
      cttgtgcaa gtggactat ccaaatcaa ccgtcgata 
      cttccgaaa gtattccca tggctcagt cacaaatgg 
      ctatctcta ccgttgagg aactcccaa gatctgaag 
      aacagttaa gagatgtaa taaaaatgc agctctctt 
      cgttacgta acatgaaaa aaagtagtg gctacctcc 
      cagatctta acaggaaat cgatatttt ttcacaatc 
      tgagaaaga aacgaaaat cgtgcgacg taaaaacga 
      ttaaacctg acacagaac gtgaaaata atgaagaag 
      agggaaaga tacttgaag aaaattgca aggaaactg 
      taactaatg atgattctt tagtgtcct tgtcagatc 
      aaacagatt ttcttattc ttgatgtca ctggaggaa 
      ttgataggt taactccgg agataaatg aaaatgata 
      acgaaaaaa ctgaagagc gatgctttg ctgctgtta 
      gtagtagtg gtgttgagg acgtgccaa ttgaaagta 
      atactgagt tacctactg taaataata tcgaagatg 
      ctacgaaga attactcac ctagtaaag aaattagag 
      accttctta caagttatc ggaaaaaaa tgtctttaa 
      attacttcg acgctaatt tttctacac tgaaaggtc 
      acgacaagt tcaaaaaaa cgcaagatc aacaccaaa 
      acaagtcaa gtgaagatg atagtccag ctcctgaaa 
      ctccaagca aagtagtgc tcatttcag tgaagaaaa 
      acatttctg aaaacttca atgaggatc ctggaataa 
      aaagatccg tcaaaaata acgaaaaat ccccgtttg 
      taagcgact ctttggaag atttcctaa aaaatgaaa 
      gtgctcagt tgaaaatta gttacccta tcccgaaat 
      taggagccg aagaaggaa tcaacgaaa gtaccaaag 
      agatcagcc cttttgaca gcatggtca aagtgcaaa 
      aaatagaac cggtcatag agaaggaaa ctccccaaa 
      gagagcgta aactaatca aggcaaaaa ctttagctg 
      acatgagga gacaatttg aaaaattaa acgcgaagg 
      aagcgaaaa aacgagaaa aaaaggaaa taaagagtg 
      agggcccat atgttccat ttcaccgca atagtcata 
      tcatttaca ataacaaga aattgtggg tgactacta 
      aaactagca ctagttgcg cgggtgtta tcggctcat 
      caaacagtg aacagttct cacccccaa gtaacaaaa 
      aactgtcac ttctctcaa cattacacc aacgaccga 
      agaggagtg ttagttaca agcctggac ctctcagca 
      agaaatgta aactacaag atgccttca gtcgggata 
      agtggacaa ctgaactga aagctttgc cccagattt 
      acttggagg tgacgccac agttcaaca aaccgattg 
      atgcggacg tggttcact tggtgccaa tttttgatg 
      attgtgtca taaccgagg aaagaacaa atttcgctt 
      tgacagctt tgaagacgg acagcgaac caaagtcgt 
      acactttcc ccatcccat acaaaaaca accaaacgc 
      acctaattg tacgaaaga gagtagaac cagttgcgg 
      ctactacac cggttgctg aagcagatc cagaaaaaa 
      ttgttgcaa gtgtgctta acgacctta tcagttatg 
      tcgaagtca aggaaattg aagacagtt taataaagg 
      aagacgatg ttgttttgg aagaaccag agccggttc 
      ctgacggaa ctcccaaat ctagaggta gagggcgca 
      ggcttaagc ggaataaag tcgacatgg aactacttc 
      gacttagtt gtaaagtca tcgatgtcg cgacgaacg 
      gaaccgaag acaagcctt gttccaaat cttactgta 
      aaatggggt gtgtgtgca ggagtttac tttgtgaaa 
      gcgtctttt ccgatcatt gccaaatgg ttgattgca 
      tgtttggat gcaagtgtc ctccgagcg atacttcaa 
      gtaactcac tgcatgttc acaccgtca accatttgg 
      aagataaag ctaagaaga acttggcaa aactggaac 
      gagaattta cccaaactg taatccaca ctgacaatg 
      acacgattc ttgttggat caggcaaag tgagaagat 
      gcacaaaaa caccaaaga aatacaccg attatattg 
      gcgacgtgg acgatgttt ttgtaaaac ctgaacccg 
      acgttgaat acaagaggc caaaagtta agaaatgtt 
      cagtggttt tggaacggc tgaatttga ggaacatta 
      aacctcttt gcctctttc atagaatgt acgagtgtg 
      gtagatgtg ttaacaggt taccacttg tcgataaac 
      cgtattgtt ttgttcata atgtattca actgtgcgt 
      gtaacaaag acacaaatg tgaggaaaa ctaattacg 
      aagtcgtca aatataaga cgggtgaaa gttgtgcac 
      gaatccgta aagtttttt cacagtata aagaacgga 
      ataaagatc ctatataca atgcaaagt tgcgaaaga 
      agaatccat attctttcc ttccgcaag tcatgaaag 
      agactgagg tggcaagta aaccaacat taatacaat 
      ttcaaagta agcaaggaa gtaagcgca aacagtctt 
      ttgttgttc gtgaggagg aaccagtcg atccaggaa 
      aagaaacag tcctgtcct atcgagata atcaaactg 
      atgaagaca gggagatac aagttgttt ctccaaata 
      aagaatttc atttcgttt ctccagatg accttcaag 
      ccgtttccc cggataaag aatttgtcc ttcagaggc 
      ggaggaaaa agaagctag taatggaaa cacagtttc 
      agaaagcaa ataatggta ttttcgacg gagaaatca 
      aactttccc tcaaagaaa accaatgct tgatacctt 
      ttgtccaca accaacctt ccttagagt acctccgaa 
      ttttaccat ggacaaact tgattaaaa actacaaca 
      cccgcaaaa tgtccattt gggcgaagg aaagtggaa 
      caacgattc tctgcaacg ctgccggtt atctcccac 
      cgagaaact tcgtcaaca ttagaagat taaaacata 
      aatcggaat ttatcaact ggatcgtct caaacacct 
      taccggacg acatacctc gccacctta tgcacgtca 
      tattatcgc caaggaaga accactaca tgatttccg 
      gttactgct taaagaaag ctgaagatt tgtccgcta 
      gacatggag caagagaga cacaagctt ctaccagtt 
      ttgagaatc tcctaacaa atgtggaca gctacgaaa 
      ccaattgtg gcaaaacgg acgatctta gtctaaact 
      cgcttctta aaaatcaca gcacgttta agttttcgc 
      gactgtata agtcggtgt tactgaaac cgaagtcga 
      aacctgtgg taaagctca agacagcta ctacgtcca 
      taaaaccag tgtcagggg gcgcaatgg aagtgaacg 
      tgctcccac ttcccagac gcccctcac accgggaca 
      ctgagacta ttgaaattg attgtggct ctacttcgc 
      aagatgggg tttcaagac caacgagtg atgtttacg 
      atttggacg aagtcactt cgcgactac caaaaaaat 
      tgtccgacg atacgaact caagtgctc tagtgccgg 
      agagaactt tgaaatgca cacacaaag acatgcaca 
      accataaga agactcttg ttttgacca tcaaagagg 
      tgcccaaac gtcggattt acttcgata aagacagtc 
      cgggggatg attttattg tgccactgc caagagttc 
      ataagtttt gtcggtgga ggatgattt atctgaatt 
      ccgacttca gttacctca acttcacca aatcaaact 
      actccatta aatacacag atttgttgg atgttgtga 
      aaatggcaa gtacggtga gatcaacga taacgcttc 
      gcaatcagg agatacgac agttttata aaacttaca 
      cgtttggga ttttttgcg ttcctgacc tccaagacc 
      gcatctttg tgggtcctt acctcaaaa cagagaacc 
      acaatgttg aaacgttga ggtacttac aagggaagt 
      tgatcgaca ctgagacgt tcaataaga taattggga 
      agaaattta acgaacgaa gccctgtct ggctttatg 
      aaacgctac ctcataaca gcatacccg acggacttg 
      tgatagaca taactgaag acgatgagg tccctaatg 
      acgtttcat cagaaccct catccagga atctgcttt 
      gtccaccgt tcgttacag cattaagtg acgaagagt 
      tgaaggcga agcacagta ttaacaggt acatcgtta 
      ctaatatcc cttacttgg gatatttcg gtgcttgcc 
      agcgcgaca gcaaagaaa ttgaggtct tttggccca 
      acaatacaa gaggttacc ggtttccaa acgtgaccc 
      ttgccaaaa atttgttaa tacactact tgtcccaaa 
      ttttctgcc ccatccccc aaacgttca aaatcaatg 
      ttataataa tgacgcagc tagacctaa agacaaaca 
      cgccggtca aagccagat atttgaccg aacctggac 
      cctttttct gcggcgagt ttggtatct acgagaagg 
      agagtgttc ccgaagacc tgctccaag agtgtaacg 
      tgccgtcgc tcgactcac ttttacaaa cgtataaag 
      aatctctag aaaatgacg aatcaattg tcaagttgt 
      tcggtttgt tcctaatgt cgaacgagg agttgcgga 
      agttccctg agccccaac aaatcatac aatgggcgc 
      ggactgaaa tttacatcc aggagaaac tctccgata 
      attacgaat cggaaaaaa gcgatttga aatcgatga 
      ttagcagca aaatgcaac gaataaaac tgttgcgga 
      gaggaataa gagacgatc aaatgagca gtaaagtgt 
      cggaaacta tcgagattc cggattccg atgatgaag 
      atcgccacg tgtcgaaag tattcgtaa tgcaatcgg 
      cacaaaaag gaaaaagtt ttgtgctga aacagaata 
      tatcagtac tcaaagaga aagacgcca aacaatcgc 
      cagtgaaaa atctcggaa tgccagttg taaataaag 
      ttatttgat aatccaata ttgttactt attgttgta 
      aagaagcta aataattaa ttatccaca cccacactg 
      acaatgatt taagtgatc gaagactgg aactggatg 
      tttgtgtta ttaattttt atatattag tttccttgt 
      atttgtaac gtgggaaga ccctgcaat aatgtgatt 
      attagtgtt tagtttcat tgaataaat ttgtttacg 
      agca
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