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Gene information
General information
TC007190 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7870366..7874383
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007190
    iBeetle screen iB_09928

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007190 RA
      MFRINHLLQ LKNVTIKIL QRSPVEASR CLALQRVQF 
      THPTRFYSK SSDDGNKKV QDGKNKACE EKKTQKRPK 
      LVLKECKPV DIHPCTPPP PPPPSKLPH INPEDCPVK 
      PTLESECPQ AKQEPPPEC PHDEGSKWD NYKNAALVG 
      LLIAAATLG ALYYVGYFE DKSKPKKIV AAKKKKTKR 
      APVKDPLHS KEIPNQVPY LLIGGGTAS FAAFRAIKS 
      ADPTAKVLV VTNESFHPY MRPPLSKEM WFNEDEESV 
      KKLIFKQWN GSERNLYYE PEDFYIKCQ DLLKNENGG 
      VAVARGYKV TRLDVVNRI AYLEDGYAI EYNKCLIAT 
      GATPKMLNV FEAACADPQ IDEKVKVFR NIYDFEEVY 
      DAFEESKSI AIIGGGFLG SELACALAR KGKRKKKTV 
      YQVFREHGN MGKILPEYL SSWTTNKVK AEGVNVVNN 
      NEVIGVKHE DGKLVLILS DGKMIRVDN VILAVGVEP 
      NTELAQKSG LEVDPELGG FLVNTELMA RSNLYIAGD 
      CACFYDVKL GRRRVEHHD HAVVTGRLA GENMTGGIK 
      PYLHQSMFW SDLGPDVGY EAIGIVDSS LPTVGVFAK 
      ASDKDTPKA VVTETGEGI RSKMEAQEQ KTGAETEHK 
      EEKKDKKED YGKGVIFYL RDDVVVGIV LWNVFNRMS 
      IARQVLKDE RKYDDLNEV AKLFNIHED
    CDS sequence
    • >TC007190 RA
      atgttccgg attaaccat ctcttgcaa ctaaagaac 
      gtaacaatc aaaatcctc cagagatcc ccagttgag 
      gcctcccga tgtctagcc cttcagcgg gtccagttt 
      acccacccg acacggttc tattcaaaa agtagcgac 
      gatgggaac aaaaaggta caagatggt aaaaacaaa 
      gcttgtgaa gagaaaaaa acccaaaaa cgccctaaa 
      ctggtcttg aaagagtgt aaacctgtt gatatccat 
      ccttgcacc ccgccccca cctcctcca ccatcgaaa 
      ctgccccac attaatcca gaggattgt ccagtcaaa 
      cccactctg gaaagcgaa tgtccacaa gctaaacag 
      gagcccccg ccagagtgc ccgcatgac gagggaagc 
      aagtgggat aattacaaa aatgccgcc cttgtgggg 
      cttctaata gcagcagcg acgctagga gctctgtat 
      tacgttgga tactttgaa gataaaagc aaaccgaaa 
      aaaatagtg gcggcaaag aaaaagaaa actaagaga 
      gcacctgtt aaagaccct ctacattca aaagagatc 
      ccaaatcaa gttccttat ttactgatc gggggcgga 
      actgcgtca tttgcagcc ttccgtgca atcaaatca 
      gctgatcca accgcaaaa gttttagtt gtgacaaat 
      gaatcgttt cacccttac atgcgtccc ccgttatcg 
      aaagaaatg tggttcaat gaggacgaa gaatcagtc 
      aagaagctc atttttaaa cagtggaac ggatcggaa 
      cgaaacctt tactacgag cctgaagac ttttatatc 
      aagtgtcaa gacttgcta aagaacgaa aatgggggc 
      gtagccgta gctcgaggc tacaaagta acccgtctt 
      gacgtcgtc aaccgaatt gcttatctc gaagacggc 
      tacgccatt gaatacaac aagtgcctc atcgccaca 
      ggtgctacc ccaaaaatg ctcaacgtg ttcgaggca 
      gcttgtgcc gacccccaa atcgacgaa aaagtcaaa 
      gtgttccgc aacatctac gactttgag gaggtctac 
      gatgctttc gaggagtca aaatccatc gcaataatt 
      ggtggtggg ttccttggc agcgaacta gcttgcgct 
      ttagctcgc aaaggtaag agaaaaaag aaaacggtg 
      taccaagtt ttccgcgag catggcaac atggggaaa 
      atcctcccc gaatacctc agttcttgg accactaat 
      aaagtcaaa gctgagggt gttaacgtc gtgaacaac 
      aacgaagtc atcggagtg aagcacgag gacggcaag 
      ctcgtccta attttaagc gatggcaaa atgataaga 
      gttgataat gtaattctg gccgttggt gtggaaccg 
      aacaccgag ctggcgcag aaatccggt ttggaagtt 
      gatccggag ctcggaggg tttttggtt aatacggag 
      ctgatggct cggtctaat ttgtacatt gcgggcgat 
      tgcgcgtgt ttttacgat gtcaagttg ggccgtcga 
      cgcgtggag caccatgac catgcggtg gtcactggc 
      cgcctagct ggggagaat atgaccggt gggattaag 
      ccctacctc caccaaagc atgttttgg tcggatttg 
      gggccggat gttgggtat gaagcgata ggaattgtc 
      gattcgtcg cttcccact gttggggtt tttgccaaa 
      gcttcggat aaagatacg cccaaggct gtagttacg 
      gagaccggg gaagggatc aggtcgaag atggaagca 
      caagagcag aagactggg gccgagact gagcacaag 
      gaggagaag aaggacaag aaggaggat tacggcaaa 
      ggggttatt ttttatctg agggatgat gtggttgta 
      ggaattgta ctgtggaat gtgtttaat cgaatgtcg 
      attgccaga caggttctc aaggatgag aggaaatat 
      gacgatctg aatgaagta gcgaagctg tttaacatt 
      cacgaagac taa
    mRNA sequence
    • >TC007190 RA
      acataatat tcatatcaa cttgaacat tttccacac 
      agttcatca aaaactgtg taactgact catgtgcct 
      tttaatctt tttatttac cgtttcgtg aattcttgg 
      acgagtttg aggttatgt tttccctga tgaaaaacg 
      tgagctcaa aaattcggc aaaaagtgt tagtgttgg 
      ttctaatcc tctaataat tgttgcaaa acgactacg 
      ggacctcac agttacagt atttctcat caagactga 
      aaaatttca cataatagt gtcattttt acttttgtc 
      atttatcat tcagctcag caatgcttt tcaattaaa 
      ttctcccaa cttccacct cgacgcatt taagaaatg 
      ttccggatt aaccatctc ttgcaacta aagaacgta 
      acaatcaaa atcctccag agatcccca gttgaggcc 
      tcccgatgt ctagccctt cagcgggtc cagtttacc 
      cacccgaca cggttctat tcaaaaagt agcgacgat 
      gggaacaaa aaggtacaa gatggtaaa aacaaagct 
      tgtgaagag aaaaaaacc caaaaacgc cctaaactg 
      gtcttgaaa gagtgtaaa cctgttgat atccatcct 
      tgcaccccg cccccacct cctccacca tcgaaactg 
      ccccacatt aatccagag gattgtcca gtcaaaccc 
      actctggaa agcgaatgt ccacaagct aaacaggag 
      cccccgcca gagtgcccg catgacgag ggaagcaag 
      tgggataat tacaaaaat gccgccctt gtggggctt 
      ctaatagca gcagcgacg ctaggagct ctgtattac 
      gttggatac tttgaagat aaaagcaaa ccgaaaaaa 
      atagtggcg gcaaagaaa aagaaaact aagagagca 
      cctgttaaa gaccctcta cattcaaaa gagatccca 
      aatcaagtt ccttattta ctgatcggg ggcggaact 
      gcgtcattt gcagccttc cgtgcaatc aaatcagct 
      gatccaacc gcaaaagtt ttagttgtg acaaatgaa 
      tcgtttcac ccttacatg cgtcccccg ttatcgaaa 
      gaaatgtgg ttcaatgag gacgaagaa tcagtcaag 
      aagctcatt tttaaacag tggaacgga tcggaacga 
      aacctttac tacgagcct gaagacttt tatatcaag 
      tgtcaagac ttgctaaag aacgaaaat gggggcgta 
      gccgtagct cgaggctac aaagtaacc cgtcttgac 
      gtcgtcaac cgaattgct tatctcgaa gacggctac 
      gccattgaa tacaacaag tgcctcatc gccacaggt 
      gctacccca aaaatgctc aacgtgttc gaggcagct 
      tgtgccgac ccccaaatc gacgaaaaa gtcaaagtg 
      ttccgcaac atctacgac tttgaggag gtctacgat 
      gctttcgag gagtcaaaa tccatcgca ataattggt 
      ggtgggttc cttggcagc gaactagct tgcgcttta 
      gctcgcaaa ggtaagaga aaaaagaaa acggtgtac 
      caagttttc cgcgagcat ggcaacatg gggaaaatc 
      ctccccgaa tacctcagt tcttggacc actaataaa 
      gtcaaagct gagggtgtt aacgtcgtg aacaacaac 
      gaagtcatc ggagtgaag cacgaggac ggcaagctc 
      gtcctaatt ttaagcgat ggcaaaatg ataagagtt 
      gataatgta attctggcc gttggtgtg gaaccgaac 
      accgagctg gcgcagaaa tccggtttg gaagttgat 
      ccggagctc ggagggttt ttggttaat acggagctg 
      atggctcgg tctaatttg tacattgcg ggcgattgc 
      gcgtgtttt tacgatgtc aagttgggc cgtcgacgc 
      gtggagcac catgaccat gcggtggtc actggccgc 
      ctagctggg gagaatatg accggtggg attaagccc 
      tacctccac caaagcatg ttttggtcg gatttgggg 
      ccggatgtt gggtatgaa gcgatagga attgtcgat 
      tcgtcgctt cccactgtt ggggttttt gccaaagct 
      tcggataaa gatacgccc aaggctgta gttacggag 
      accggggaa gggatcagg tcgaagatg gaagcacaa 
      gagcagaag actggggcc gagactgag cacaaggag 
      gagaagaag gacaagaag gaggattac ggcaaaggg 
      gttattttt tatctgagg gatgatgtg gttgtagga 
      attgtactg tggaatgtg tttaatcga atgtcgatt 
      gccagacag gttctcaag gatgagagg aaatatgac 
      gatctgaat gaagtagcg aagctgttt aacattcac 
      gaagactaa cattccggc attcggcga ttaatttta 
      atttgataa gaagtttag atcgatttg agtttgttc 
      aaatgtata tagaaagga taaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY