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Gene information
General information
TC008270 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7624745..7629089
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008270
    iBeetle screen iB_09924

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands potentially content darker - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum potentially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum posterior constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum potentially size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    vertex posterior labrum bristle potentially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008270 RA
      MMDILRCCE TTENEITAF VDGTLLQDR VLKNMLQSE 
      YRCVPSNVF INQREITES MIEIVGGWM MEVCEEQTC 
      QDDVFLLSM NYLYRFLTT TNIKKNQLQ LLGAACMLI 
      ASKLREPKP LSAEMLVFY TDHSITTNM LTSWELLVL 
      SKLKWDIIA IVPVDFLPH LLVRLDFER LGIKAEMVK 
      KHAKILITL CAKECRFTN CYPSLLACA SVVSALCGL 
      GWVNKYHQS QEALLKTLA DIIDVQETD FMQELVQNI 
      DQMIKQNFE NVKDNKVCD EVTRSEQCT PPPGKIRDY 
      KTATTPTDV HDVCF
    CDS sequence
    • >TC008270 RA
      atgatggat atactgcgt tgttgcgaa acaaccgaa 
      aatgaaatt acggccttc gttgacggc actttgttg 
      caagaccgg gtcttaaaa aacatgtta cagtctgaa 
      taccggtgt gtgccctcc aacgtgttt attaatcaa 
      cgggagatc accgagtct atgatcgag atcgtcggg 
      ggttggatg atggaggta tgcgaagaa cagacgtgt 
      caagacgac gtcttcctc ctctccatg aactacctg 
      tacaggttc ctcaccacc accaacatc aagaagaac 
      cagctgcag ctgctgggg gcggcgtgc atgctcatc 
      gcctccaag ttaagggag cccaaacct ttatcagcc 
      gagatgttg gttttctat accgaccac agcattact 
      accaacatg ctcacgtcc tgggagctg ctggtgctc 
      agcaagctg aaatgggac atcatcgcc atcgtgccg 
      gtggacttc ctgccgcac ctgctggtg cgcctggac 
      ttcgagcgg ctgggcatc aaagccgag atggtcaag 
      aagcacgca aagatcctc ataaccctc tgcgcaaaa 
      gaatgccga tttacgaat tgttatccg agtctgctg 
      gcttgtgct agcgtggtg tcggcgctg tgcggccta 
      ggatgggtc aacaagtac caccaatcg caagaagcc 
      ctcctcaag acactagcg gatattatc gacgtgcaa 
      gaaacggat ttcatgcaa gagttggtg cagaacatc 
      gaccaaatg atcaaacag aatttcgag aacgttaaa 
      gacaataaa gtgtgtgac gaagtgacg aggtcagaa 
      caatgtact cctcctcct ggaaaaatt cgcgactac 
      aagacggcg actacgccg accgatgtg catgatgtt 
      tgtttttga
    mRNA sequence
    • >TC008270 RA
      ggtaagtta aaatcattg gttacttaa caccagtaa 
      cagaaagac ggtaactga aaccaaacc aaaactttt 
      ctccaagta aagccccat gcaacccta agtccttgt 
      atgtgaaaa aaattcttg tgtgtagtg ttttaaaaa 
      aactgtgct ttaaagaaa aatgatgga tatactgcg 
      ttgttgcga aacaaccga aaatgaaat tacggcctt 
      cgttgacgg cactttgtt gcaagaccg ggtcttaaa 
      aaacatgtt acagtctga ataccggtg tgtgccctc 
      caacgtgtt tattaatca acgggagat caccgagtc 
      tatgatcga gatcgtcgg gggttggat gatggaggt 
      atgcgaaga acagacgtg tcaagacga cgtcttcct 
      cctctccat gaactacct gtacaggtt cctcaccac 
      caccaacat caagaagaa ccagctgca gctgctggg 
      ggcggcgtg catgctcat cgcctccaa gttaaggga 
      gcccaaacc tttatcagc cgagatgtt ggttttcta 
      taccgacca cagcattac taccaacat gctcacgtc 
      ctgggagct gctggtgct cagcaagct gaaatggga 
      catcatcgc catcgtgcc ggtggactt cctgccgca 
      cctgctggt gcgcctgga cttcgagcg gctgggcat 
      caaagccga gatggtcaa gaagcacgc aaagatcct 
      cataaccct ctgcgcaaa agaatgccg atttacgaa 
      ttgttatcc gagtctgct ggcttgtgc tagcgtggt 
      gtcggcgct gtgcggcct aggatgggt caacaagta 
      ccaccaatc gcaagaagc cctcctcaa gacactagc 
      ggatattat cgacgtgca agaaacgga tttcatgca 
      agagttggt gcagaacat cgaccaaat gatcaaaca 
      gaatttcga gaacgttaa agacaataa agtgtgtga 
      cgaagtgac gaggtcaga acaatgtac tcctcctcc 
      tggaaaaat tcgcgacta caagacggc gactacgcc 
      gaccgatgt gcatgatgt ttgtttttg aaaactata 
      gtttaattg tgaatagaa gtgaatcct gcgatgtcg 
      ctagttcga aggaaagtg tgcaaagac gtgatggct 
      ttaggggaa ggcaataaa ttacgcttg aataaaaga 
      aaaaagaac tgttcaatt tctgtacgt ctttttaga 
      aatacaaga cttttacag cgggtgaac gttgaaaat 
      gtttgagat atactatgt tagtcagtt acgatccta 
      agtttctgt tcctaaaac gtgattcga aacgcaaat 
      taaattaag caagtttaa caatttgtt tagaagctt 
      gcgtagtat gaataacct tcaaagact gctagctct 
      aatccggag caatgactt acttaatta tcgttaatc 
      gtcttccaa atttgacca agtcaatca atgcaatca 
      tgatgagaa aagtatttt tgtcaaact attaaattt 
      ttatagtta gtttattaa gaaagtgtg attctaact 
      gtaggtatt ttcaatttt atatgtgat caactaaga 
      atagtgatt ttaaaattt atttgacaa atatcttat 
      tataagggc cttgaacgt acctaaggt tggctttac 
      ccaaactcc acgccatgc aaattcacg gaatgtgac 
      ctttgcctg gcttggggt ttcgataaa accagcata 
      atatgcttg tgcatacga ctatatagc ttattaagg 
      caacactgt aaatattta ggaggtagc caattaagc 
      actttgaca ttgtacata aatgaccgt ttccaccaa 
      ttacactgc caataacta tttataaat tttgctcac 
      aatagtcca atattccat atacctcat tttagctct 
      cattacaac aacaaaagc cctgtgaat ctatatatt 
      gtaactggt attggtttg ttccaccga catttccac 
      gcttgatac ttccaaaac cttcctaaa gcaggggaa 
      tcttggtgt aacaaaccc tcccagaaa aaactatta 
      tattgtaca tagtatata gcgccgatt agcgcacga 
      tcaaagaca ttttgtgct tcctcaaac ttccgtttt 
      tctcaaaac gcgaattta agtcactat acgccccag 
      aattcctct gatcgtgcg ttaaatact tatattgta 
      aatagatat ataccacac agaattcta attgtttac 
      attcattat aagttaaat tttataaat acaaataaa 
      aacctcgat aaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY