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Gene information
General information
TC007203 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7566911..7580743
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007203
    iBeetle screen iB_09923

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007203 RA
      MSRLLSLRT CRRTLETPR IIVVSASRR FSFTLFYHG 
      ESRRLHAET RQTDECRTF FKMLTLVWP CLVFSAVVA 
      GSRLQCNRT LEGVSPKPN PGHFQIEIS GNPATYVPG 
      EQYTVFLRA TTDEKFAEF MLSVENEKR KSPPPSIQL 
      YGDSLTKFS EECSSTVIS TDSQPKSEI QVLWLAPAS 
      GTGCVTFKG AVVISDGVW CGEAGSLTK TLCENSQDN 
      DDIQPVILK HCCACDEAN DIIGASHTV NYTFWRYGD 
      IASEGLRQL AENGNTRML ESELKAKSD HIRTIIKAR 
      GISYPNITG KTFAVFRVD NRHHLMSIV SMIDPSPDW 
      IVGVSSLEL CLRNCSWVE SKILNLYPW DAGTDDGIT 
      YISPNQPSS PRQPIKRIT ATSPSDPRS PFYDKSGAE 
      MKPLARLYL SRQRLYEKL CDPSIVEDY DDSDGCETE 
      EWSDWSPCS ATCGRGVKY KQRRYKNEE SKYVCHKKL 
      TERAGCEAV QKYCPHQPV REVEDPKCV LSEWSEWSS 
      CSVTCGKGT KTRDRKYKN RLAAKSCAA AKVNPPKLQ 
      ENLECYGEG KCDDLEEEV VDCPQRPWS EWSPCSVTC 
      GKGFKVRYR LSLQSQNWP FVPNSDSYD ETEEFDEDD 
      PCSHQKVKE TVECFQKSC EQEEELPEK GYVCSLPKD 
      VGACKSNTD RWYFDNIKG NCEIFSYSG CDGNSNNFK 
      TLEQCQKVC ADYQKELIA NLTAHKRQL DVTISGVLS 
      YNIQNDAIN VSAPSYRPY SQIQLINST DCVLTKWSS 
      WSHCNVTGH CGSGYRTKY RQIKVHPTD GGKPCSKRL 
      TKKKKCHVP CTNDLGKTK YNFNTFDNV RVDCVMSPW 
      TNWTKCDPR CFREQFQER YRRVVVHPT PNGTPCASK 
      VEKRRCFCK GLN
    CDS sequence
    • >TC007203 RA
      atgagtcgt ttacttagt cttcgtacg tgcaggcgc 
      acattggaa accccacgc ataatagtg gtgtcggcg 
      tcgcgacgc ttcagtttc acgctgttt taccacggg 
      gaaagtcga cgcctgcac gcggaaacg cgccaaacg 
      gacgagtgt cgcacattt ttcaagatg ctaacactg 
      gtctggccc tgcttggta ttcagcgct gtggtcgcc 
      ggcagtcgt ctgcagtgc aacaggacc ttggaaggc 
      gtctcgccg aagccaaat cctggtcac tttcaaatt 
      gaaatctcc ggaaacccc gccacttac gttccggga 
      gaacaatac accgtattt ttgcgtgcg acaactgac 
      gagaaattc gccgaattc atgttaagc gtcgaaaat 
      gaaaaacgc aaatctcca cctccctcg atccaatta 
      tacggcgat tccttgacg aaattcagc gaggaatgc 
      tccagtacc gtcatatca accgattcg caacccaaa 
      agcgaaatc caggtcctg tggttagcc ccagcctcc 
      ggcaccggc tgcgttacg ttcaagggg gctgtggtg 
      atctccgat ggggtgtgg tgcggggag gccgggtct 
      ctgaccaaa acgctgtgc gaaaactcg caagacaac 
      gacgatatc cagcccgtt atcctcaag cactgttgt 
      gcgtgcgat gaggcgaac gacatcatc ggggcctcg 
      cacacggtg aattacacc ttctggagg tacggggac 
      attgccagc gagggtctc cgccaactg gcggagaac 
      ggcaacaca aggatgctc gaaagcgaa ttaaaagca 
      aaaagcgat cacattagg accatcatc aaagcgcga 
      ggtataagt tatccgaat attacaggg aaaacgttc 
      gctgtgttc agggttgac aatcgacac catcttatg 
      tctattgtc tcaatgatt gatccctcg cccgactgg 
      attgtcgga gtttccagt ttggagttg tgtctaagg 
      aattgttct tgggtggaa tcaaagata ttgaatttg 
      tacccgtgg gatgcgggc acggatgat gggataacc 
      tacatttcc cctaatcag ccgtcctct cctcgccag 
      cccataaaa cgcatcaca gcgaccagt ccgagcgac 
      cctcgctcc ccattttac gacaaaagc ggtgccgag 
      atgaaacct ctggcccga ctctatttg tcccgccaa 
      cgcctctac gaaaagtta tgcgatcct tcgatcgtc 
      gaagattac gacgacagt gacggttgc gagaccgaa 
      gaatggtcc gactggtct ccctgctct gccacttgc 
      ggcagaggc gtcaagtac aaacagcga cgctacaaa 
      aacgaggaa agcaaatac gtctgccac aagaaactc 
      acggagcgg gcagggtgc gaagccgtg cagaaatac 
      tgcccgcac cagcccgtc cgcgaggtg gaggacccc 
      aagtgcgtc ttgagcgaa tggagcgag tggtccagc 
      tgcagtgtc acgtgtggt aagggcacc aagacccga 
      gataggaaa tacaagaat cgccttgcg gcgaagagt 
      tgcgctgcg gcgaaggtc aacccgcca aagctgcag 
      gagaacttg gagtgctac ggggaggga aagtgcgat 
      gatttggag gaggaagtg gtggactgc ccgcagagg 
      ccctggagc gagtggtcg ccctgttcg gtgacttgt 
      gggaaaggg tttaaagtg aggtacagg ctatctttg 
      caatcgcaa aactggccg tttgttccc aactcggac 
      agttacgac gaaaccgag gaatttgac gaggatgac 
      ccgtgtagc caccagaaa gttaaggag acggtggag 
      tgctttcag aagtcgtgc gaacaggag gaggagctg 
      cctgagaaa ggctacgtt tgcagtttg ccgaaagac 
      gtcggggcg tgcaaaagc aacactgat agatggtac 
      ttcgacaac attaaggga aattgcgag atatttagt 
      tacagcggt tgtgatgga aattcaaac aatttcaaa 
      accctcgag cagtgtcag aaagtttgt gcggactac 
      caaaaagaa ttaatcgcg aaccttacg gctcataaa 
      cgccaactc gacgtaacg atttcgggg gtcctttct 
      tacaacatc cagaacgac gcaatcaat gtttcagcg 
      ccctcgtat cgaccctac agccagata caattaatt 
      aattcgaca gactgcgtt ctcaccaag tggtccagc 
      tggagccac tgcaatgtc acggggcat tgtggtagc 
      ggatataga acgaaatat agacaaatc aaagttcac 
      ccgactgat ggcgggaag ccgtgctcg aagagatta 
      acgaagaag aaaaaatgt cacgttcca tgcaccaac 
      gacttgggc aagaccaaa tacaatttc aatactttc 
      gataacgtc agggtcgat tgtgtcatg tcgccgtgg 
      acgaattgg accaagtgc gacccaaga tgcttcagg 
      gaacagttc caggaaaga tacagacgc gtggtggtt 
      catcccact cccaatggc acgccctgc gcctccaaa 
      gtcgaaaag cggcgctgc ttctgcaaa ggactcaac 
      taa
    mRNA sequence
    • >TC007203 RA
      tgacattta ttaattctt gtgcaagga tcaaacagt 
      ttggcagct ctcaagggg aattttatt aaatcgctc 
      gtgtttcaa ggaaattca atctaattt agtcgcagt 
      gtgatgagt cgtttactt agtcttcgt acgtgcagg 
      cgcacattg gaaacccca cgcataata gtggtgtcg 
      gcgtcgcga cgcttcagt ttcacgctg ttttaccac 
      ggggaaagt cgacgcctg cacgcggaa acgcgccaa 
      acggacgag tgtcgcaca tttttcaag atgctaaca 
      ctggtctgg ccctgcttg gtattcagc gctgtggtc 
      gccggcagt cgtctgcag tgcaacagg accttggaa 
      ggcgtctcg ccgaagcca aatcctggt cactttcaa 
      attgaaatc tccggaaac cccgccact tacgttccg 
      ggagaacaa tacaccgta tttttgcgt gcgacaact 
      gacgagaaa ttcgccgaa ttcatgtta agcgtcgaa 
      aatgaaaaa cgcaaatct ccacctccc tcgatccaa 
      ttatacggc gattccttg acgaaattc agcgaggaa 
      tgctccagt accgtcata tcaaccgat tcgcaaccc 
      aaaagcgaa atccaggtc ctgtggtta gccccagcc 
      tccggcacc ggctgcgtt acgttcaag ggggctgtg 
      gtgatctcc gatggggtg tggtgcggg gaggccggg 
      tctctgacc aaaacgctg tgcgaaaac tcgcaagac 
      aacgacgat atccagccc gttatcctc aagcactgt 
      tgtgcgtgc gatgaggcg aacgacatc atcggggcc 
      tcgcacacg gtgaattac accttctgg aggtacggg 
      gacattgcc agcgagggt ctccgccaa ctggcggag 
      aacggcaac acaaggatg ctcgaaagc gaattaaaa 
      gcaaaaagc gatcacatt aggaccatc atcaaagcg 
      cgaggtata agttatccg aatattaca gggaaaacg 
      ttcgctgtg ttcagggtt gacaatcga caccatctt 
      atgtctatt gtctcaatg attgatccc tcgcccgac 
      tggattgtc ggagtttcc agtttggag ttgtgtcta 
      aggaattgt tcttgggtg gaatcaaag atattgaat 
      ttgtacccg tgggatgcg ggcacggat gatgggata 
      acctacatt tcccctaat cagccgtcc tctcctcgc 
      cagcccata aaacgcatc acagcgacc agtccgagc 
      gaccctcgc tccccattt tacgacaaa agcggtgcc 
      gagatgaaa cctctggcc cgactctat ttgtcccgc 
      caacgcctc tacgaaaag ttatgcgat ccttcgatc 
      gtcgaagat tacgacgac agtgacggt tgcgagacc 
      gaagaatgg tccgactgg tctccctgc tctgccact 
      tgcggcaga ggcgtcaag tacaaacag cgacgctac 
      aaaaacgag gaaagcaaa tacgtctgc cacaagaaa 
      ctcacggag cgggcaggg tgcgaagcc gtgcagaaa 
      tactgcccg caccagccc gtccgcgag gtggaggac 
      cccaagtgc gtcttgagc gaatggagc gagtggtcc 
      agctgcagt gtcacgtgt ggtaagggc accaagacc 
      cgagatagg aaatacaag aatcgcctt gcggcgaag 
      agttgcgct gcggcgaag gtcaacccg ccaaagctg 
      caggagaac ttggagtgc tacggggag ggaaagtgc 
      gatgatttg gaggaggaa gtggtggac tgcccgcag 
      aggccctgg agcgagtgg tcgccctgt tcggtgact 
      tgtgggaaa gggtttaaa gtgaggtac aggctatct 
      ttgcaatcg caaaactgg ccgtttgtt cccaactcg 
      gacagttac gacgaaacc gaggaattt gacgaggat 
      gacccgtgt agccaccag aaagttaag gagacggtg 
      gagtgcttt cagaagtcg tgcgaacag gaggaggag 
      ctgcctgag aaaggctac gtttgcagt ttgccgaaa 
      gacgtcggg gcgtgcaaa agcaacact gatagatgg 
      tacttcgac aacattaag ggaaattgc gagatattt 
      agttacagc ggttgtgat ggaaattca aacaatttc 
      aaaaccctc gagcagtgt cagaaagtt tgtgcggac 
      taccaaaaa gaattaatc gcgaacctt acggctcat 
      aaacgccaa ctcgacgta acgatttcg ggggtcctt 
      tcttacaac atccagaac gacgcaatc aatgtttca 
      gcgccctcg tatcgaccc tacagccag atacaatta 
      attaattcg acagactgc gttctcacc aagtggtcc 
      agctggagc cactgcaat gtcacgggg cattgtggt 
      agcggatat agaacgaaa tatagacaa atcaaagtt 
      cacccgact gatggcggg aagccgtgc tcgaagaga 
      ttaacgaag aagaaaaaa tgtcacgtt ccatgcacc 
      aacgacttg ggcaagacc aaatacaat ttcaatact 
      ttcgataac gtcagggtc gattgtgtc atgtcgccg 
      tggacgaat tggaccaag tgcgaccca agatgcttc 
      agggaacag ttccaggaa agatacaga cgcgtggtg 
      gttcatccc actcccaat ggcacgccc tgcgcctcc 
      aaagtcgaa aagcggcgc tgcttctgc aaaggactc 
      aactaattt tcattgcat atcacaagc aacgaacag 
      ctgaaattt agtttatta ttaaaattg tgacaataa 
      ctttcaaat cacttttat gtgaaaagt accaaattg 
      tttgtgtat tttatttct tgtggtaga tgtggaatt 
      attttttat taaatgtga tctaaattc tgaatttat 
      tcgtttaat aaaaacatt taactttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY