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Gene information
General information
TC007208 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7518581..7519723
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007208
    iBeetle screen iB_09922

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007208 RA
      MSGEVQKIL KNADCVCFD VDSTVIQEE 
      GIDELAKFC NKGAEVANL TAKAMTGTM 
      TFQESLKLR LDIIQPTLT QIRDFLKTK 
      PPTLTPGVK NLINLLHSR KIPVYLISG 
      GFKCIIAPI AAQLNIPYD HIYANRLKF 
      YYSGEHAGF DENAPTSRS GGKAVVIDF 
      LKKQHNYKN VILIGDGAT DLEASPPAD 
      GFIGYGGNV IRPTVQAKA KWFVTDFNE 
      VIDVLNQ
    CDS sequence
    • >TC007208 RA
      atgagtgga gaagtccaa aaaatatta aaaaatgcg 
      gattgcgtc tgtttcgat gtcgactcg accgtaatc 
      caggaagaa gggatcgac gaactcgcc aagttttgc 
      aacaagggc gctgaagtt gcaaattta acggccaaa 
      gcaatgaca ggaacgatg acctttcaa gaatcgctt 
      aagctgcgc ctggatatt atacaacca acattgacg 
      caaatccgg gattttttg aaaaccaaa ccacccact 
      ttgactccg ggggtcaag aatttgata aacttgttg 
      cacagtcgt aaaattcca gtttattta atttctggt 
      ggttttaaa tgtattatt gcaccaatt gcggcacag 
      ttgaacatt ccctacgac cacatttat gccaatcgg 
      ctgaaattt tactactca ggggaacat gccgggttt 
      gatgaaaat gctccaact tcaagaagt ggtggcaaa 
      gccgttgtt atcgacttt ttgaaaaaa caacacaat 
      tataaaaat gttatactg ataggtgat ggggctacc 
      gatttagaa gcgtctccc ccagctgat ggttttatc 
      gggtacggt ggaaatgtc ataagaccg acggttcaa 
      gcgaaagca aaatggttc gtaaccgat tttaacgaa 
      gttatagac gttttaaat caatga
    mRNA sequence
    • >TC007208 RA
      cgcaacata caaaaaata tattttgca atctaagaa 
      tcaaaattt ttatcgtgt agttgtgtt tacaagtat 
      tgttaactc gctccaacg attaagaaa gacataact 
      tatcacttt gaccttcga gtgttgtgc atagtattt 
      cgtttagac caaagtcgt gactcccca gtaacaggt 
      cgaaattag tctcaaaga acaaaatga gtggagaag 
      tccaaaaaa tattaaaaa atgcggatt gcgtctgtt 
      tcgatgtcg actcgaccg taatccagg aagaaggga 
      tcgacgaac tcgccaagt tttgcaaca agggcgctg 
      aagttgcaa atttaacgg ccaaagcaa tgacaggaa 
      cgatgacct ttcaagaat cgcttaagc tgcgcctgg 
      atattatac aaccaacat tgacgcaaa tccgggatt 
      ttttgaaaa ccaaaccac ccactttga ctccggggg 
      tcaagaatt tgataaact tgttgcaca gtcgtaaaa 
      ttccagttt atttaattt ctggtggtt ttaaatgta 
      ttattgcac caattgcgg cacagttga acattccct 
      acgaccaca tttatgcca atcggctga aattttact 
      actcagggg aacatgccg ggtttgatg aaaatgctc 
      caacttcaa gaagtggtg gcaaagccg ttgttatcg 
      actttttga aaaaacaac acaattata aaaatgtta 
      tactgatag gtgatgggg ctaccgatt tagaagcgt 
      ctcccccag ctgatggtt ttatcgggt acggtggaa 
      atgtcataa gaccgacgg ttcaagcga aagcaaaat 
      ggttcgtaa ccgatttta acgaagtta tagacgttt 
      taaatcaat gattgtaaa aaatgttta agtttagcg 
      ctgcgggtc actgccacc tgttacatt acgcaataa 
      aaattccct aacatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY