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Gene information
General information
TC008264 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7500074..7502158
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008264
    iBeetle screen iB_09921

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber mostly not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber potentially degenerated - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008264 RA
      MATGFSFGA KTTAPPTFG TGSITFGTP ANQPSQQFG 
      FGNAPTSTP NLFGSQPAT STPSTGFGF GATTPGFGA 
      AAQNPGSTS FGFGAPAAN TGLSFGTPA STAASTGLA 
      FKPPASTGL SFATPASTG LSFGTPAST GLSFGTPAA 
      STGLSFGAP ASTGLTFGT STSAPSTGL SFGASTSAP 
      STGLSFGAP ASTQSTGLS FGAPASTPA TGFNFGATT 
      SASTLGLSF GATSTAAPS LSFGGATTT TGLSFGTGS 
      SGLGGLGSL GTSSTGLNL TSGATTTTT QSVGLGGIA 
      STQSKPGGS SSAQKELPP KEQLLPNEF SQLVEQFKN 
      IVQEEKNRS SDVARCSVK EFRKVESEL DSLNHQFNQ 
      VENQLLNNR SLAEQLKYD TAKGLQNIE MAQRTQDTP 
      PSLQYDNTA PLQFFLELA DTFEKELQT LKLKIDNTE 
      KFVKKCSEP NVLTSQDLA LGLKRLHET FVALAGRMQ 
      SIHSQVETQ KEVFLSLRK QAIKDNINP FEKMDRNME 
      AMHTVMKNA LKATPPNLA SGPTPFNSI ALGSNNLAI 
      ASQQTQPTF PSTTTSLGF GLGNTAPTF GTPASNTSM 
      FKPTGFGSS PFQLNTSPN FQLQKPPTG NKRGKQ
    CDS sequence
    • >TC008264 RA
      atggcaact gggtttagt tttggggcc aaaacaacc 
      gcgcccccc acgtttggt actggttcc ataacattt 
      ggaaccccg gcaaaccaa ccaagtcag cagttcggt 
      tttggaaac gcccccacg tccactccc aatttattc 
      ggatcccag cccgctacc tcaacccct agcactggt 
      ttcggattt ggagcaaca acccctggc tttggggcc 
      gcagctcaa aaccctgga tccaccagt tttggtttt 
      ggggctccg gctgcaaac acagggctg tcttttggt 
      accccagct tcaacagcg gcttccacc ggtttagcg 
      ttcaaaccc ccagcttca acggggctc agttttgcc 
      actcccgcc tcaacgggg cttagtttt ggaacccca 
      gcttccaca ggcttaagt tttggtact ccagctgcc 
      tcaacgggg ctcagtttt ggggctccg gcctcaacc 
      ggtctgact ttcggtact agcacttct gctccaagt 
      actggactt agtttcggg gctagtacc tcagccccg 
      agcacggga ctgtcgttt ggggcccca gcatcgaca 
      caaagcacg gggcttagt tttggggcc cctgcgtcg 
      actcctgcc accggtttt aattttggg gccacaact 
      tcggcctct actcttggc ttgagtttt ggggctacg 
      agtaccgca gcgccgagt ttaagtttt gggggtgcc 
      accaccacc acagggctt agttttggg actgggagt 
      agtggttta gggggactt gggagtctc gggacgtct 
      tcaactggt ctaaattta acgtcaggg gctactact 
      accactaca caaagtgtg ggtttgggg ggtatagct 
      tcgactcag tctaaacct gggggtagt tcctcggca 
      cagaaagaa ttaccacca aaggaacaa ctgttaccg 
      aatgaattt tcccagttg gtggaacaa tttaaaaat 
      attgttcag gaagagaaa aaccggagt tcggacgtt 
      gcgcgttgt tctgttaaa gaatttcga aaagttgaa 
      tcagagttg gattctttg aaccaccag tttaatcaa 
      gttgaaaac cagttgttg aataatcgt agtcttgct 
      gaacagtta aagtatgac actgccaag ggtttgcaa 
      aatattgaa atggctcaa cgtactcaa gacacgccc 
      ccgagtctt caatacgat aacacagcc cccttacaa 
      tttttccta gaacttgcg gacacgttt gaaaaggaa 
      ttgcaaacg ttaaaacta aaaattgac aatactgag 
      aagttcgtc aagaaatgt agcgaacca aatgttcta 
      acatcacaa gatctagcc ttgggttta aaacgactc 
      catgagact tttgtggct ttggcaggc cgtatgcag 
      tcaattcat agtcaagtc gaaacccag aaagaagtt 
      ttcctaagt ttgcgtaag caagcgatt aaagacaat 
      attaacccg ttcgaaaaa atggatagg aacatggag 
      gccatgcat acagttatg aaaaatgca ctcaaggct 
      acgcccccg aacctggct tcggggccc acgcccttc 
      aacagcata gcccttggg agcaacaat ttggcgatt 
      gcgagtcaa caaacccaa ccgacgttt ccgtcaaca 
      actacaagt ttaggtttt ggtttagga aatacagcc 
      ccgactttc ggaacccca gcttcaaac acttcaatg 
      ttcaaaccg acaggtttt gggtcctct ccttttcag 
      ttaaatacg tctccaaat tttcaattg caaaaaccc 
      ccaactggt aataaaaga ggcaaacag tag
    mRNA sequence
    • >TC008264 RA
      agttttgta gtttttgct gttgttgta ttattcaag 
      acagaaatg gcaactggg tttagtttt ggggccaaa 
      acaaccgcg ccccccacg tttggtact ggttccata 
      acatttgga accccggca aaccaacca agtcagcag 
      ttcggtttt ggaaacgcc cccacgtcc actcccaat 
      ttattcgga tcccagccc gctacctca acccctagc 
      actggtttc ggatttgga gcaacaacc cctggcttt 
      ggggccgca gctcaaaac cctggatcc accagtttt 
      ggttttggg gctccggct gcaaacaca gggctgtct 
      tttggtacc ccagcttca acagcggct tccaccggt 
      ttagcgttc aaaccccca gcttcaacg gggctcagt 
      tttgccact cccgcctca acggggctt agttttgga 
      accccagct tccacaggc ttaagtttt ggtactcca 
      gctgcctca acggggctc agttttggg gctccggcc 
      tcaaccggt ctgactttc ggtactagc acttctgct 
      ccaagtact ggacttagt ttcggggct agtacctca 
      gccccgagc acgggactg tcgtttggg gccccagca 
      tcgacacaa agcacgggg cttagtttt ggggcccct 
      gcgtcgact cctgccacc ggttttaat tttggggcc 
      acaacttcg gcctctact cttggcttg agttttggg 
      gctacgagt accgcagcg ccgagttta agttttggg 
      ggtgccacc accaccaca gggcttagt tttgggact 
      gggagtagt ggtttaggg ggacttggg agtctcggg 
      acgtcttca actggtcta aatttaacg tcaggggct 
      actactacc actacacaa agtgtgggt ttggggggt 
      atagcttcg actcagtct aaacctggg ggtagttcc 
      tcggcacag aaagaatta ccaccaaag gaacaactg 
      ttaccgaat gaattttcc cagttggtg gaacaattt 
      aaaaatatt gttcaggaa gagaaaaac cggagttcg 
      gacgttgcg cgttgttct gttaaagaa tttcgaaaa 
      gttgaatca gagttggat tctttgaac caccagttt 
      aatcaagtt gaaaaccag ttgttgaat aatcgtagt 
      cttgctgaa cagttaaag tatgacact gccaagggt 
      ttgcaaaat attgaaatg gctcaacgt actcaagac 
      acgcccccg agtcttcaa tacgataac acagccccc 
      ttacaattt ttcctagaa cttgcggac acgtttgaa 
      aaggaattg caaacgtta aaactaaaa attgacaat 
      actgagaag ttcgtcaag aaatgtagc gaaccaaat 
      gttctaaca tcacaagat ctagccttg ggtttaaaa 
      cgactccat gagactttt gtggctttg gcaggccgt 
      atgcagtca attcatagt caagtcgaa acccagaaa 
      gaagttttc ctaagtttg cgtaagcaa gcgattaaa 
      gacaatatt aacccgttc gaaaaaatg gataggaac 
      atggaggcc atgcataca gttatgaaa aatgcactc 
      aaggctacg cccccgaac ctggcttcg gggcccacg 
      cccttcaac agcatagcc cttgggagc aacaatttg 
      gcgattgcg agtcaacaa acccaaccg acgtttccg 
      tcaacaact acaagttta ggttttggt ttaggaaat 
      acagccccg actttcgga accccagct tcaaacact 
      tcaatgttc aaaccgaca ggttttggg tcctctcct 
      tttcagtta aatacgtct ccaaatttt caattgcaa 
      aaaccccca actggtaat aaaagaggc aaacagtag 
      atgataaag ttttattgt aatagatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY