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Gene information
General information
TC007211 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7498488..7499982
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007211
    iBeetle screen iB_09920

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007211 RA
      MFKNHLVFK TVPHLIRRN ILNKNFSSI SQILDKSGV 
      GDVITVKGW IKSLRDQKE HVFFDVSDG STAKKLQIL 
      LPKKSKPED LSTGASVVV SGPLTLSPK GQLELTANS 
      IEVCGKCVV SEGYPFAPR KQYAAEYIR QYLHFRPRT 
      HKFGALLRL RNAATLAIY NHFNSEGFV NVHTPVLTS 
      NDCEGAGEV FKVVPDNKK LIKSMAKES QAEDEVYFN 
      QKSFLTVSG QLHLEAVAH GLSKVYTFG PTFRAENSK 
      SRLHLSEFY MLEGEIAFV EKLDGLMTC IEQLIKQVT 
      KKLVNDSIE DIEKCQEKK VSFDWLDKS FPVVTYKEA 
      VTILSQNKD KFSENFDSK DGFGKEHEM FLVEYFGKV 
      PTFVIDWPK DMKPFYMKD NGDGTVAAL DLLGPNVGE 
      VVGGSLREN NYEKLLEKI PNSNGHLDW YLDLRKFGS 
      VPTGGFGLG FERYLQLIL GISNIKDTI PFPRWPHNC 
      SL
    CDS sequence
    • >TC007211 RA
      atgtttaaa aaccattta gtttttaaa actgtgcct 
      catttaatt cgcagaaac attctaaac aaaaatttc 
      tcttcaatc tctcagata cttgacaaa agtggtgtt 
      ggcgatgtc ataacagtg aagggatgg ataaaatcg 
      ttacgggac cagaaagaa catgtgttt ttcgacgta 
      agcgatggt tcaactgcc aaaaaactc caaattttg 
      cttcctaaa aaatcaaaa ccggaagat ctatcgacc 
      ggggcttcc gtagtagtt tcaggaccc cttactcta 
      tcaccgaaa ggccagttg gaattaacg gcaaacagt 
      atcgaagtc tgcggtaaa tgcgtcgta agcgaaggc 
      tatcctttt gcgccacgt aaacagtac gccgctgaa 
      tatatcagg cagtatctc cacttcagg ccaagaaca 
      cacaaattt ggggcactt ttgcgatta cggaatgcg 
      gcaacgttg gctatttat aatcatttt aattcggaa 
      ggttttgtt aatgtgcat acgccagtc ttgacttct 
      aacgattgt gaaggggct ggagaggtg tttaaagta 
      gtccctgac aataaaaag ttgatcaaa tcaatggcg 
      aaagagagc caagctgaa gatgaggtc tactttaat 
      cagaaatcg tttttgacg gtctcgggg cagttgcat 
      ttagaagct gtagctcat ggtttaagc aaagtttac 
      actttcggg cccactttt agggccgaa aattccaaa 
      tcaagactg catttgtcc gaattttat atgttggag 
      ggagaaatt gcgtttgtg gaaaaacta gatgggctt 
      atgacctgc atagaacaa ttaattaaa caagttaca 
      aaaaaacta gtcaatgat tcaattgaa gacatcgaa 
      aaatgccaa gagaaaaaa gtgtcgttc gattggttg 
      gataaaagt tttcctgtg gttacgtac aaagaagcg 
      gtaacaatt ttaagtcag aataaagat aagtttagc 
      gaaaatttt gattccaaa gatggtttt ggtaaagaa 
      catgagatg ttcctggtt gaatatttc ggaaaagtg 
      cccactttt gtcatagac tggccaaaa gacatgaaa 
      cccttttat atgaaagac aatggggac ggaaccgtg 
      gccgcactt gacttattg gggcctaat gttggcgag 
      gtcgttggc ggaagtctg cgtgaaaat aattacgag 
      aaattgcta gaaaaaatc cccaattcc aatgggcac 
      ttggactgg tatttggac ttgaggaaa tttggatcg 
      gtgccaact ggcggattt ggtttaggt tttgaaaga 
      tatttgcaa ctaattttg ggaataagc aatatcaaa 
      gacacgatt ccctttccc agatggccg cataattgc 
      agtttgtag
    mRNA sequence
    • >TC007211 RA
      aatatgttt aaaaaccat ttagttttt aaaactgtg 
      cctcattta attcgcaga aacattcta aacaaaaat 
      ttctcttca atctctcag atacttgac aaaagtggt 
      gttggcgat gtcataaca gtgaaggga tggataaaa 
      tcgttacgg gaccagaaa gaacatgtg tttttcgac 
      gtaagcgat ggttcaact gccaaaaaa ctccaaatt 
      ttgcttcct aaaaaatca aaaccggaa gatctatcg 
      accggggct tccgtagta gtttcagga ccccttact 
      ctatcaccg aaaggccag ttggaatta acggcaaac 
      agtatcgaa gtctgcggt aaatgcgtc gtaagcgaa 
      ggctatcct tttgcgcca cgtaaacag tacgccgct 
      gaatatatc aggcagtat ctccacttc aggccaaga 
      acacacaaa tttggggca cttttgcga ttacggaat 
      gcggcaacg ttggctatt tataatcat tttaattcg 
      gaaggtttt gttaatgtg catacgcca gtcttgact 
      tctaacgat tgtgaaggg gctggagag gtgtttaaa 
      gtagtccct gacaataaa aagttgatc aaatcaatg 
      gcgaaagag agccaagct gaagatgag gtctacttt 
      aatcagaaa tcgtttttg acggtctcg gggcagttg 
      catttagaa gctgtagct catggttta agcaaagtt 
      tacactttc gggcccact tttagggcc gaaaattcc 
      aaatcaaga ctgcatttg tccgaattt tatatgttg 
      gagggagaa attgcgttt gtggaaaaa ctagatggg 
      cttatgacc tgcatagaa caattaatt aaacaagtt 
      acaaaaaaa ctagtcaat gattcaatt gaagacatc 
      gaaaaatgc caagagaaa aaagtgtcg ttcgattgg 
      ttggataaa agttttcct gtggttacg tacaaagaa 
      gcggtaaca attttaagt cagaataaa gataagttt 
      agcgaaaat tttgattcc aaagatggt tttggtaaa 
      gaacatgag atgttcctg gttgaatat ttcggaaaa 
      gtgcccact tttgtcata gactggcca aaagacatg 
      aaacccttt tatatgaaa gacaatggg gacggaacc 
      gtggccgca cttgactta ttggggcct aatgttggc 
      gaggtcgtt ggcggaagt ctgcgtgaa aataattac 
      gagaaattg ctagaaaaa atccccaat tccaatggg 
      cacttggac tggtatttg gacttgagg aaatttgga 
      tcggtgcca actggcgga tttggttta ggttttgaa 
      agatatttg caactaatt ttgggaata agcaatatc 
      aaagacacg attcccttt cccagatgg ccgcataat 
      tgcagtttg tagctgagt taagaaata aaacactta 
      ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY