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Gene information
General information
TC007247 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6945911..6950548
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007247
    iBeetle screen iB_09913

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007247 RA
      MPCCTPTCR KWSVFGFGT FFLVLGVAI IVFWNSLVQ 
      SIKDQELNL GNDGTTEYK MWKETPIPM YIEFYLYNW 
      TNWKEVVDS KWSLKPSFE EHGPYTYNE KHIRKNVIF 
      NDNHTVTYK TQRIWHFAP EKSKGSLDD VITTLNPIL 
      VTVGSMVKY KHPIVKMGV NFFIKEKGV NLTVTKTAR 
      EFIFDGYDD PLLDLLKKL HMRHINIPF DKFAWFISR 
      NESIDYDGI YNMYDGTDD VRKLGRFAW WNYNNQTEY 
      FPNYCGEVN GTSGELWYP VENDQYAEV FSPDTCSTL 
      TLVKQGTEE LHGVVGHKF VGDEKLFDN GTRYPDMRC 
      FSPGDVLPS GVRNVSHCK FGAPAFISY PHFYLADPY 
      YREAITGMT PNKTEHELF ISIEPETGI PLHARVAAQ 
      INLHLEKID RITLLEHVG REYLIPAMW FKQYAVLSE 
      DLANQAKML IILPAVGQY TGIGAVALG SLLLSIFGF 
      LTWKSYRKG REEETLLNQ EEF
    CDS sequence
    • >TC007247 RA
      atgccttgc tgtacaccc acttgccga aaatggagc 
      gtatttggt ttcggtacg ttctttcta gtcctcggt 
      gttgccatt atcgttttt tggaacagc ttggtccag 
      tcgattaaa gatcaggaa ctaaatctc ggtaacgat 
      ggtacgacg gagtacaaa atgtggaag gaaacgccg 
      attccgatg tacatcgag ttttattta tacaattgg 
      actaattgg aaagaagtc gttgattct aaatggtcg 
      ctaaaaccg tcgtttgaa gaacacggt ccttacact 
      tataacgaa aaacatatt cgtaaaaat gtgatattc 
      aacgacaat cacacggtg acttacaag acccaaagg 
      atttggcat tttgctccc gaaaagtct aaagggtcc 
      ttagatgac gtaataacg acactgaac ccgatttta 
      gttacggtc ggttctatg gtcaagtac aaacaccct 
      attgttaaa atgggcgtc aattttttt atcaaagaa 
      aagggggtt aatttaaca gtgactaaa acagcgcgg 
      gaatttatt tttgacgga tacgacgac ccgttgctg 
      gatttattg aagaaatta cacatgagg catataaat 
      ataccgttc gacaagttt gcctggttt atttcgagg 
      aatgagtcc attgattac gacggcatt tacaacatg 
      tatgacggc accgacgac gtccgaaaa ctcggaagg 
      ttcgcttgg tggaattac aacaaccaa accgaatat 
      ttcccgaat tattgcggg gaagtcaat ggaactagc 
      ggggaatta tggtatcca gtggaaaac gaccaatat 
      gcagaagtt ttttcaccg gacacgtgc agtacttta 
      actttagtc aaacaagga acggaggaa ttgcacggc 
      gtagtgggc cacaaattc gtgggcgat gagaaattg 
      tttgataat gggacacgt tatccggac atgagatgt 
      ttcagtcct ggggatgtg ttaccaagc ggcgtccga 
      aacgtgtcg cattgcaag tttggagca ccggcgttt 
      atttcctac ccgcatttt tacttagct gatccgtac 
      tatagggaa gctatcacg ggaatgact cccaacaaa 
      accgagcac gaattgttt atttcaatt gaaccggaa 
      accggtatc ccattacat gcaagagtg gcagctcag 
      attaatctt catcttgaa aaaatcgac agaataacg 
      ttgttggaa cacgttggg agagagtat ttgattccg 
      gcgatgtgg tttaaacag tatgccgtt ttgagcgaa 
      gacttggca aatcaggct aagatgctt atcattctt 
      cctgctgtt ggtcaatat accggaatt ggggctgtc 
      gccttgggt tcactgctt ttgagcatt tttggtttt 
      cttacgtgg aaaagttac aggaaaggt agagaggag 
      gaaacactg ttgaatcag gaagaattt taa
    mRNA sequence
    • >TC007247 RA
      agaaggtcg agctgttat tgttgttcc aattttttg 
      ttactcatc gacgctaat gttctgggt gttgatgtg 
      tctgaaaac tattgtata aaaactttc tggatcaag 
      attaatgtg gacgtatct gtgatattt cttgtagga 
      ttataagaa aataatgcc ttgctgtac acccacttg 
      ccgaaaatg gagcgtatt tggtttcgg tacgttctt 
      tctagtcct cggtgttgc cattatcgt tttttggaa 
      cagcttggt ccagtcgat taaagatca ggaactaaa 
      tctcggtaa cgatggtac gacggagta caaaatgtg 
      gaaggaaac gccgattcc gatgtacat cgagtttta 
      tttatacaa ttggactaa ttggaaaga agtcgttga 
      ttctaaatg gtcgctaaa accgtcgtt tgaagaaca 
      cggtcctta cacttataa cgaaaaaca tattcgtaa 
      aaatgtgat attcaacga caatcacac ggtgactta 
      caagaccca aaggatttg gcattttgc tcccgaaaa 
      gtctaaagg gtccttaga tgacgtaat aacgacact 
      gaacccgat tttagttac ggtcggttc tatggtcaa 
      gtacaaaca ccctattgt taaaatggg cgtcaattt 
      ttttatcaa agaaaaggg ggttaattt aacagtgac 
      taaaacagc gcgggaatt tatttttga cggatacga 
      cgacccgtt gctggattt attgaagaa attacacat 
      gaggcatat aaatatacc gttcgacaa gtttgcctg 
      gtttatttc gaggaatga gtccattga ttacgacgg 
      catttacaa catgtatga cggcaccga cgacgtccg 
      aaaactcgg aaggttcgc ttggtggaa ttacaacaa 
      ccaaaccga atatttccc gaattattg cggggaagt 
      caatggaac tagcgggga attatggta tccagtgga 
      aaacgacca atatgcaga agttttttc accggacac 
      gtgcagtac tttaacttt agtcaaaca aggaacgga 
      ggaattgca cggcgtagt gggccacaa attcgtggg 
      cgatgagaa attgtttga taatgggac acgttatcc 
      ggacatgag atgtttcag tcctgggga tgtgttacc 
      aagcggcgt ccgaaacgt gtcgcattg caagtttgg 
      agcaccggc gtttatttc ctacccgca tttttactt 
      agctgatcc gtactatag ggaagctat cacgggaat 
      gactcccaa caaaaccga gcacgaatt gtttatttc 
      aattgaacc ggaaaccgg tatcccatt acatgcaag 
      agtggcagc tcagattaa tcttcatct tgaaaaaat 
      cgacagaat aacgttgtt ggaacacgt tgggagaga 
      gtatttgat tccggcgat gtggtttaa acagtatgc 
      cgttttgag cgaagactt ggcaaatca ggctaagat 
      gcttatcat tcttcctgc tgttggtca atataccgg 
      aattggggc tgtcgcctt gggttcact gcttttgag 
      catttttgg ttttcttac gtggaaaag ttacaggaa 
      aggtagaga ggaggaaac actgttgaa tcaggaaga 
      attttaata ctctgattt tttaatagc cagacgtaa 
      aattggaat ttagcaata cttgtaaat attgaacat 
      tgctgtgat atgtacttg accattttg tgtaagact 
      agtgtgggc attgattgc atgtgtata ttctgtaaa 
      attatgtac atagtactt aatttgaaa ttcataaat 
      gaatgaatg aaatatatt atttttagc cagacatac 
      aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY