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Gene information
General information
TC008217 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6898330..6899537
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008217
    iBeetle screen iB_09912

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008217 RA
      MGDESKNSS EEPSQVNQD ELILAQQRQ IEKEISESI 
      PLVGEMEPI SSLNSEYAN DEVYLEKIK DLSSKYKHI 
      RRTRPDGNC FFRAYSYAG LERLLDKKD EFNAFYKQA 
      EESKDALVQ LGFQQFTVE DFYDIYMEV LKRVGDLTD 
      VDDAKKTLH FLFNEQGYS DYMVVYLRL LTSGQLQKD 
      QDFYSCFIE GDRTVADFC HQEVEPMYK ESDHIHIIA 
      ACAALNMGV RVVYMDRGN NKSVTEHDF PEGCTPLVH 
      LLYRPGHYD ILYP
    CDS sequence
    • >TC008217 RA
      atgggcgac gaaagcaaa aattcctca gaagaaccc 
      tctcaggtg aaccaggac gaactgatc ctggcccaa 
      caacgccag attgaaaag gagatttct gaaagtatt 
      cctttagtt ggtgaaatg gaaccaatt tcttcgtta 
      aatagcgaa tacgctaac gatgaagtt tacttagaa 
      aaaatcaaa gatctgtct tccaagtac aagcacatt 
      cgtagaact aggcctgac gggaactgt tttttccgt 
      gcgtatagt tatgcgggt cttgagaga ctgttagac 
      aaaaaagac gaatttaac gcgttttat aagcaagct 
      gaggagagt aaagacgct ctagtgcag cttggcttt 
      cagcagttt actgtagaa gatttttac gatatttac 
      atggaagtc ctcaaacgt gtaggtgac ctaaccgac 
      gtagacgac gccaagaaa actcttcat tttttattt 
      aacgagcaa ggttattcc gattacatg gttgtttat 
      ttaagactt ttaacaagc ggacagttg caaaaagac 
      caggatttc tacagctgt ttcattgag ggtgacaga 
      actgttgct gatttttgt caccaggaa gttgaacca 
      atgtacaaa gaaagtgac cacattcac ataattgct 
      gcttgtgct gcccttaat atgggggtg cgtgtggtt 
      tacatggac aggggtaac aacaagtcg gtcacagaa 
      cacgatttt cctgaaggt tgcacacct ttagtacat 
      ttactttac cgtccaggg cattacgat attttgtat 
      ccttaa
    mRNA sequence
    • >TC008217 RA
      cgctcaact ttatccgtg ttattgttt ttactaatt 
      attttggag cgcgtatta ttgttgagt tggttttta 
      agacagtta ttgattatt gatgaattt ggggttgtt 
      ttttatcat aactgccat aaacttgtc tcagcaact 
      gtcaaaatt gagtggttg aaaacattc gtgcttggt 
      gtggttatt ttgtgttag ttttaacag aaaatgggc 
      gacgaaagc aaaaattcc tcagaagaa ccctctcag 
      gtgaaccag gacgaactg atcctggcc caacaacgc 
      cagattgaa aaggagatt tctgaaagt attccttta 
      gttggtgaa atggaacca atttcttcg ttaaatagc 
      gaatacgct aacgatgaa gtttactta gaaaaaatc 
      aaagatctg tcttccaag tacaagcac attcgtaga 
      actaggcct gacgggaac tgttttttc cgtgcgtat 
      agttatgcg ggtcttgag agactgtta gacaaaaaa 
      gacgaattt aacgcgttt tataagcaa gctgaggag 
      agtaaagac gctctagtg cagcttggc tttcagcag 
      tttactgta gaagatttt tacgatatt tacatggaa 
      gtcctcaaa cgtgtaggt gacctaacc gacgtagac 
      gacgccaag aaaactctt catttttta tttaacgag 
      caaggttat tccgattac atggttgtt tatttaaga 
      cttttaaca agcggacag ttgcaaaaa gaccaggat 
      ttctacagc tgtttcatt gagggtgac agaactgtt 
      gctgatttt tgtcaccag gaagttgaa ccaatgtac 
      aaagaaagt gaccacatt cacataatt gctgcttgt 
      gctgccctt aatatgggg gtgcgtgtg gtttacatg 
      gacaggggt aacaacaag tcggtcaca gaacacgat 
      tttcctgaa ggttgcaca cctttagta catttactt 
      taccgtcca gggcattac gatattttg tatccttaa 
      ttttcaacg tttcattta taatttcta cagttcgag 
      gaataaaag agttgttga taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY