Status
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Gene information
General information
TC007292 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6470303..6474937
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007292
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
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      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
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      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_09910

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Adult morphology
      abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 4 (13 dpi)
      Days post injection



      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007292 RA
        MGGRHTLHI VGAVLILIQ ATCCLQVKK FPLLMPNVY 
        PDREESYFC TPVRVVPDK SFYIVGFEP VATMDVVHH 
        MIVFGCSKP GTTDPFWDC GEMASDSSS TLRKANPCA 
        EGSNVIYAW ALNAKPLQL PENVGFQVG EGSKIQYLV 
        LQIHYSKKF KENESDNSG LNLIYTEEP QSKLAGVLL 
        LGTGGAIPP RSVTHMETL CQIREKKTI YPIAYRTHT 
        HSLGQVVSG YSVRRDKSG VDHWSLLGK RNPNTPQMF 
        YPIFNKGPI RYGDRLAAR CTMDSTNRT DYTIVGPKH 
        DNEMCNFYI IYYVEEGTP LDMKYCISE GPPYFYWRN 
        EENDLNNIP DDEASNLI
      CDS sequence
      • >TC007292 RA
        atgggagga agacatacg ttacacatt gtgggtgca 
        gttttaatt ttaatacaa gcaacatgt tgtcttcaa 
        gtgaaaaaa ttcccctta cttatgccc aatgtctat 
        cctgacagg gaagaatca tacttctgc actcccgta 
        cgcgtagtc cctgacaaa agtttttac atcgtcggt 
        tttgagccg gttgcgaca atggatgtc gtccaccac 
        atgattgtt tttggatgc tcaaaacct ggaaccact 
        gatccgttt tgggattgc ggggaaatg gcgtcggat 
        tctagttca actttacga aaagctaat ccttgcgca 
        gaaggctca aatgtaatt tatgcctgg gctttgaat 
        gcaaaacca ctgcaactt cccgaaaac gttggcttt 
        caagtcggg gaaggttca aaaattcag tacttagtc 
        ctacaaatt cattactcc aagaaattt aaagaaaat 
        gaaagtgat aactccggc ttgaatctc atatacacg 
        gaggaacca caaagtaaa ttagctgga gttttgttg 
        ctgggaact ggcggtgct attcctccc cgttctgta 
        acacacatg gagacgctc tgtcaaatt agggagaag 
        aaaactatt tatcccatt gcctataga acgcatacg 
        cacagtttg ggccaagtt gtgtccggt tacagtgta 
        cgtagggac aaatctggg gtcgaccat tggtcactt 
        ttagggaaa cgcaaccct aacacgccc caaatgttt 
        tatccaatt tttaacaaa ggcccgata cgatacgga 
        gaccgactt gcggctaga tgcacgatg gatagcact 
        aaccgaacc gactacact atcgtaggc cctaaacat 
        gataatgaa atgtgcaat ttttacata atttattac 
        gtggaggaa ggaacacca ctggacatg aaatattgc 
        atatctgaa ggaccacct tatttttac tggcgcaac 
        gaggaaaac gacttgaat aacattcca gatgacgag 
        gcatccaat ttaatttaa
      mRNA sequence
      • >TC007292 RA
        gattagcac aagagttgg ttatctggg aacaaacac 
        gaccatatg tttcgtaat tgaacttcc cgcctacgt 
        gttgacgtc acaacccgg agctggtta ttgactggt 
        ccgtggttg gttactgaa agaactaaa cagaacaaa 
        atgcgaata gtaacttag atcgattag tggaagcat 
        gggaggaag acatacgtt acacattgt gggtgcagt 
        tttaatttt aatacaagc aacatgttg tcttcaagt 
        gaaaaaatt ccccttact tatgcccaa tgtctatcc 
        tgacaggga agaatcata cttctgcac tcccgtacg 
        cgtagtccc tgacaaaag tttttacat cgtcggttt 
        tgagccggt tgcgacaat ggatgtcgt ccaccacat 
        gattgtttt tggatgctc aaaacctgg aaccactga 
        tccgttttg ggattgcgg ggaaatggc gtcggattc 
        tagttcaac tttacgaaa agctaatcc ttgcgcaga 
        aggctcaaa tgtaattta tgcctgggc tttgaatgc 
        aaaaccact gcaacttcc cgaaaacgt tggctttca 
        agtcgggga aggttcaaa aattcagta cttagtcct 
        acaaattca ttactccaa gaaatttaa agaaaatga 
        aagtgataa ctccggctt gaatctcat atacacgga 
        ggaaccaca aagtaaatt agctggagt tttgttgct 
        gggaactgg cggtgctat tcctccccg ttctgtaac 
        acacatgga gacgctctg tcaaattag ggagaagaa 
        aactattta tcccattgc ctatagaac gcatacgca 
        cagtttggg ccaagttgt gtccggtta cagtgtacg 
        tagggacaa atctggggt cgaccattg gtcactttt 
        agggaaacg caaccctaa cacgcccca aatgtttta 
        tccaatttt taacaaagg cccgatacg atacggaga 
        ccgacttgc ggctagatg cacgatgga tagcactaa 
        ccgaaccga ctacactat cgtaggccc taaacatga 
        taatgaaat gtgcaattt ttacataat ttattacgt 
        ggaggaagg aacaccact ggacatgaa atattgcat 
        atctgaagg accacctta tttttactg gcgcaacga 
        ggaaaacga cttgaataa cattccaga tgacgaggc 
        atccaattt aatttaaac gaccaaagc agttgtgac 
        aattcatag tcgaaaaaa tacgaaaaa tatatctca 
        tatcgtgtt atatttttg taatcatta ttttgaaca 
        caatcttaa ccaggaatt aaccaggga attgcagta 
        ccaagctta tttatttat ttgacatca cttttgatg 
        ttttatttt ttattatta ttttaggca aggattttg 
        tatctcaag acgtaacgg cgaacaaaa caatataaa 
        cattccttg tagcacaaa atacgagta cttatatgc 
        acatttatt tgcagtgta gacggtagg taattactt 
        gatttgatt tgaattttt atctctgca ctgatgtac 
        ttattaatc atgtgacct ttccaatgt aggaacaaa 
        ttagtgaat tatataaaa tgtaaattt atgtcagca 
        tacataata aactataag aaaaaatta tgaaaaaaa 
        tatacttac ggttacatg tatactaat tttatattg 
        caaatatgt atgtatgtt ttattataa atacacgaa 
        gtatcgatt ttgtttttt atattccat atgttgatg 
        tctacatat cgagtgatt cacgtagtt gacgagccg 
        tacaagact taaatgcaa cacacaata ctctgtatt 
        cttatgtga atcacctgg tacttgtag cagtgatgg 
        ttagtgatg cgttgatgt tttaacaaa ttttaatta 
        ctgttacac gagatctcc tttattttt aatcttccc 
        aataacgaa acaaaagat ttatttatg gtaggtact 
        catcagtgc cttgcactt aaaattacc aaacgaaag 
        tcaagcgcg caaattatc gggcctaca ggtgaaact 
        caagattga aaataatgg agaattgat cagatttta 
        tccgaattt attgtagtt agacaattt taacgcgga 
        tgacatggt ttacagtcc catgagtca tagatgtac 
        ttagtatct aagctttaa ttatttttg gcaattgca 
        tgagatatt ttaaatatt ataccttcc gtttttcct 
        gattatgta gtaagttcg tgataggtg ataacactt 
        gtgtggaac gggcattgg gtgctttta aagataagg 
        caaaaatag attttcatc tttttcata tatacatcg 
        gtacagcat tgctattat cgcattttt gttcgttta 
        tttggaagt gatggtgcg agaaatttc aaggcaatg 
        tactgataa atatctaag acttattat atctaatgt 
        tacttctaa ttaaaatgt atatttgta cagagaaga 
        ataaatata tgtaaatgg a
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY