Status
Please wait ...
Gene information
General information
TC031470
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011536
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3656141..3658945
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031470
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09909

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031470 RA
      MDQLNLNAD FLKFDKENN GDVKREIDF LQCGNFNSV 
      QPSSSYLSN IFSNTLDVN ANLNNRMPL DLSNLKDLD 
      GLCKLEIIK NEIPYLVKY KLQDGKHVK IWQCKTCFK 
      EFGHQYTLM RHLPTHTDE RKFQCNTCG KAFRQMSTL 
      SQHRAIHSH ERPYVCEIC QKTFNRVST LISHRKTHT 
      GIKPHQCHL CNKAFHQKG NLRNHIFTH TNERPYKCD 
      DCGKGFNQM SNLMCHKLK SHQRMDKPK HSCQICRRS 
      FAKRIALRN HEQYEHRES NQAIDSFLT DDQKISNAI 
      FVEPIKTKA MQLAIETNQ IPFALLRPL TGIPVLVRV 
      LPAGNDKQM LVPASAEDL KKHGQISVT PKTTTPGTT 
      LEDVTEDKH NLVGSTVQI KIPVVATVI QQSGEGGHM 
      SMSVVSPGP NNEAVKQPN WQSLGSYIL TATNDEQSD 
      GQMMGMQDL NFSTDEKTN LTDYDIETS GMKHNGDAV 
      HAPDDVFII T
    CDS sequence
    • >TC031470 RA
      atggatcag ctaaacttg aacgcagat ttcttaaaa 
      ttcgacaag gagaataat ggggatgtt aagagagaa 
      atcgatttt ttgcaatgt ggaaacttt aactctgtt 
      caacctagt tcgtcgtat ttatcgaac atattttcg 
      aacacatta gacgttaat gccaattta aataatcgg 
      atgccattg gatttgagc aatttgaaa gatctagac 
      ggtttatgc aaactagaa attataaaa aatgaaata 
      ccgtacctt gtaaagtac aaactacaa gatggaaaa 
      cacgtaaaa atctggcaa tgcaaaact tgctttaag 
      gaatttggc catcaatat actttaatg cgtcacttg 
      cctacgcac actgacgaa agaaaattt cagtgcaac 
      acctgcgga aaagctttt cgacaaatg tcgacactt 
      tcgcaacac agagcaatt cactcgcat gaaaggcct 
      tacgtttgt gaaatttgc caaaaaaca tttaatcgc 
      gtctctact ttaatttct caccggaaa actcacacc 
      ggtattaaa ccgcatcag tgtcactta tgtaacaaa 
      gcttttcac caaaaagga aacctccgt aatcacatt 
      tttacccac accaatgaa aggccatac aagtgcgac 
      gactgtgga aaaggattt aaccaaatg tctaacttg 
      atgtgtcat aaattaaaa tcgcaccaa cgcatggat 
      aaacctaaa cactcgtgt caaatttgt agaagaagt 
      tttgcgaaa cgtatagct ttaagaaat cacgaacaa 
      tatgaacat agggaaagt aatcaagcc attgattca 
      tttctaaca gatgaccaa aaaattagc aacgcaata 
      tttgtggaa ccaatcaaa accaaagca atgcaacta 
      gcgatcgag acaaaccag atacccttc gctttactg 
      aggccttta acaggaatt cctgttttg gtccgagtt 
      ttaccggct gggaacgat aagcaaatg ctagttcca 
      gcaagtgct gaagatttg aagaaacat ggccagata 
      tcagttacc ccaaaaacg accacgcct ggaacaaca 
      ctcgaagat gtgaccgaa gataaacat aatcttgtt 
      ggaagtacc gttcaaatt aaaatacca gttgttgct 
      acagtaatt cagcaaagt ggtgaaggt ggtcatatg 
      tccatgtcg gtcgtaagt cctggtcct aataacgaa 
      gcagtcaaa caaccaaat tggcagtcc ttgggttcc 
      tatattttg actgcgact aatgacgaa caaagtgat 
      ggacaaatg atgggcatg caagattta aattttagt 
      acagatgaa aaaactaac ttaactgat tatgatatt 
      gaaacttct ggaatgaaa cataatggt gatgcggtc 
      catgcacca gacgatgta ttcatcatc acatag
    mRNA sequence
    • >TC031470 RA
      tttttgtga aaaatctgt ttgcattag ttacaaaag 
      ttccttcaa gctcttcta attgcttta aattggaat 
      tataagaaa tggatcagc taaacttga acgcagatt 
      tcttaaaat tcgacaagg agaataatg gggatgtta 
      agagagaaa tcgattttt tgcaatgtg gaaacttta 
      actctgttc aacctagtt cgtcgtatt tatcgaaca 
      tattttcga acacattag acgttaatg ccaatttaa 
      ataatcgga tgccattgg atttgagca atttgaaag 
      atctagacg gtttatgca aactagaaa ttataaaaa 
      atgaaatac cgtaccttg taaagtaca aactacaag 
      atggaaaac acgtaaaaa tctggcaat gcaaaactt 
      gctttaagg aatttggcc atcaatata ctttaatgc 
      gtcacttgc ctacgcaca ctgacgaaa gaaaatttc 
      agtgcaaca cctgcggaa aagcttttc gacaaatgt 
      cgacacttt cgcaacaca gagcaattc actcgcatg 
      aaaggcctt acgtttgtg aaatttgcc aaaaaacat 
      ttaatcgcg tctctactt taatttctc accggaaaa 
      ctcacaccg gtattaaac cgcatcagt gtcacttat 
      gtaacaaag cttttcacc aaaaaggaa acctccgta 
      atcacattt ttacccaca ccaatgaaa ggccataca 
      agtgcgacg actgtggaa aaggattta accaaatgt 
      ctaacttga tgtgtcata aattaaaat cgcaccaac 
      gcatggata aacctaaac actcgtgtc aaatttgta 
      gaagaagtt ttgcgaaac gtatagctt taagaaatc 
      acgaacaat atgaacata gggaaagta atcaagcca 
      ttgattcat ttctaacag atgaccaaa aaattagca 
      acgcaatat ttgtggaac caatcaaaa ccaaagcaa 
      tgcaactag cgatcgaga caaaccaga tacccttcg 
      ctttactga ggcctttaa caggaattc ctgttttgg 
      tccgagttt taccggctg ggaacgata agcaaatgc 
      tagttccag caagtgctg aagatttga agaaacatg 
      gccagatat cagttaccc caaaaacga ccacgcctg 
      gaacaacac tcgaagatg tgaccgaag ataaacata 
      atcttgttg gaagtaccg ttcaaatta aaataccag 
      ttgttgcta cagtaattc agcaaagtg gtgaaggtg 
      gtcatatgt ccatgtcgg tcgtaagtc ctggtccta 
      ataacgaag cagtcaaac aaccaaatt ggcagtcct 
      tgggttcct atattttga ctgcgacta atgacgaac 
      aaagtgatg gacaaatga tgggcatgc aagatttaa 
      attttagta cagatgaaa aaactaact taactgatt 
      atgatattg aaacttctg gaatgaaac ataatggtg 
      atgcggtcc atgcaccag acgatgtat tcatcatca 
      catagatgc atgtaattt gtttttgtt tttcaagct 
      taattattt ctaggtgat caaactttc tatgtaaat 
      agcatgtgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY