Status
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Gene information
General information
TC007325 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6162889..6168666
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007325
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09908

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007325 RA
      MNVVWVLVL LVPSVFAKL TNIETQTGQ NHETNEIED 
      VFVKQETTS GPENEASTT EIYSTTTEP TFFSKTACA 
      NTSEEQSVT TEAHIPVVN FTQINASSF EVFLQWDPP 
      ENTQCVDKY VIYLDSDFY ETTESNYTI ANLTACNEY 
      KVAIAASKN DNVGQFTND SFKTIAPNP FGYGVEDLK 
      VEQVTDYVL NLTWNAPDE KVVQCVTAY DILWWPAGS 
      TGKTKESKT NETYLELSP VVGCMKYEI NVKPLIGET 
      QQGNVQAAE FQAQAYPNN PPNIVIGSL SSTKTQISL 
      TWETDDKSK NMCNVSAIV VNCVAPEDS NRNSSWIIW 
      EAQSVREVT EQTANNRYS MTIENLSPF TNYSCSGVV 
      TNTGGNSSE TPFEATTDE DVPSAPQSL TIKDVGPQK 
      FTIEWTQPE QIPGIMTSY GLKIISVGP LDRNVNKDC 
      ANNATDHFF DLDAEDTSL VFEEALPYF KYSVSVYGV 
      DGAGNGTEA NADASTTAT KPDQVENAE TLPPEYSQN 
      ETYSGSVIV KFDPPCNTN GPFKHYEIN YIGQREGFS 
      PENQTLETT ETNATIPLK PEREYSIEI KVVTENFTS 
      GPVSVPAFQ SQAGVPVVN KNVDLKAPT PEEEKAQFA 
      FKKENFDNI NGEIIAYCL ILLTESQEG GYGFWDGTN 
      ETWPPVPKN GTQLTPNFW NPFKDGVSS ANFTIGDGG 
      CGANYCNKI LNDDTKYWL IVRALTRAG FKDSSLLPF 
      KTDVMEELN LPLILGLTF GLLLLVLLI IFLIILWRR 
      SRKSQPPEE IKRVSTTSP EPIPCQKFV TFYANLKTT 
      PHILKQQFT DLDLQSKKV AAQISYATL AENRRKNRY 
      TNILPFDET RVKLNIDED DEISSDYIN ASYIKGYSG 
      KMEYIATQG PLESTTRDF WKMVLQENV SIIVMVSQF 
      VEQSKEKCY HYFPSNHES VMFGESLEV KCCTELHFG 
      TYCMRTLQV RKGTEQRHV IHMQFLEWP DFGVPLGTD 
      NMLQFCRQL REKANSEGG LIVVHCSAG VGRTGTLIA 
      LDILLQTID DHRDINIYK TVLDLRKQR ANMVQTEKQ 
      YVFIHTCVN DYLETPIIQ SNDSSQERI YENMDVLKY 
      SLKKNLIET EVESAL
    CDS sequence
    • >TC007325 RA
      atgaacgtg gtgtgggtt ctggtgctt ctagtgccg 
      agtgttttc gcaaaattg acaaacatc gagacgcaa 
      actggccaa aaccacgaa acgaacgaa attgaagat 
      gtttttgtg aagcaagaa acaacttca gggcctgaa 
      aatgaagct tcaactact gaaatatat tcaactact 
      acagagcct acatttttt tcaaaaacg gcttgcgct 
      aacacttct gaggaacaa agtgtcaca acagaagca 
      cacattcct gtggttaat tttacacaa atcaatgcc 
      tcaagtttt gaagtgttt ctacaatgg gatcccccc 
      gaaaacaca cagtgtgtc gacaaatac gtaatttac 
      ctcgacagt gatttttac gaaaccacc gaaagtaat 
      tacactatc gctaattta acagcttgt aatgaatac 
      aaagttgca attgcggct tcgaagaat gacaacgtg 
      ggacaattt acaaacgat agttttaag acaattgct 
      cccaatccg ttcggttac ggagttgag gacttgaaa 
      gttgagcaa gtcaccgat tacgtttta aatctgaca 
      tggaatgca ccagatgaa aaggtcgtg caatgtgta 
      accgcttac gatatactt tggtggcct gccggcagt 
      acgggcaaa accaaagag agtaaaacc aacgaaacg 
      tatttagaa ttatcgcca gttgttggt tgtatgaaa 
      tatgaaata aatgtgaag cccttaatt ggagaaacc 
      caacaagga aatgtccaa gcggcggaa tttcaagct 
      caagcctat ccaaacaat cctccaaat attgtcatc 
      ggctcatta tcaagcacc aaaacacaa ataagccta 
      acttgggaa accgacgat aaatcgaag aatatgtgc 
      aatgtgagt gcaattgtg gtaaattgt gttgcacca 
      gaagattca aacagaaat agttcctgg ataatctgg 
      gaggcacaa agtgtgaga gaagtgacc gaacaaacc 
      gccaataac cgatactca atgacaatc gagaatttg 
      tcacctttt acgaactat tcatgttcg ggagttgtc 
      acaaacact ggcggaaat tcgtctgaa accccgttt 
      gaagctacg accgatgaa gacgttcct tccgctcca 
      cagtcactc acaattaaa gatgtgggg ccccaaaaa 
      ttcacaatc gaatggaca caacccgaa caaatcccc 
      ggaattatg acttcgtat ggtttgaaa atcattagt 
      gtcggacct cttgaccgg aatgtgaat aaagactgt 
      gcgaacaat gccactgat cactttttt gacctcgat 
      gctgaagat actagtctt gtttttgag gaagctttg 
      ccgtatttt aaatacagt gtgtctgtc tatggagtc 
      gatggggcc ggaaatggg acagaagcg aatgcagac 
      gcgagcaca acagccaca aagccggat caagttgaa 
      aacgcggaa actttacca ccggaatat tcccaaaac 
      gaaacttac tccggctct gtgatcgtc aaattcgac 
      cctccatgc aacacaaac ggacctttc aaacattat 
      gaaataaat tatatcgga cagagagaa ggtttcagt 
      cctgaaaac caaacactt gaaaccact gaaacaaac 
      gcaacaatt ccactaaaa cctgaacgc gaatattcg 
      attgaaatt aaagttgtg actgaaaat tttacttca 
      ggcccggtt tcagtgccg gcgtttcag tcgcaagct 
      ggtgtacct gtggttaac aaaaatgtg gacttgaaa 
      gcaccaact cctgaagaa gaaaaggca caattcgcg 
      ttcaaaaaa gaaaatttt gataacata aacggcgag 
      attattgct tattgtttg atacttttg acggaatca 
      caagaaggg ggttacggt ttttgggac gggacaaac 
      gaaacatgg cctccggta cccaaaaat ggcactcaa 
      ttaacacca aacttctgg aaccctttc aaagacgga 
      gtgagctcg gctaatttc acaattggc gatggtggt 
      tgtggtgcc aactattgc aacaaaatc ctaaatgac 
      gacacaaaa tattggttg attgtgcga gccctaaca 
      agagcgggt tttaaggac agttcatta ttacctttt 
      aaaacagat gtgatggaa gaattgaat ttgccccta 
      attctgggc ttaacattc ggtctctta cttttagta 
      cttttgata attttcttg ataattttg tggcgacgc 
      agtcggaaa agtcagcca ccggaagaa ataaaacga 
      gtttcaaca acaagccct gaacccatc ccctgccaa 
      aaattcgtc actttttac gctaatctc aaaacgact 
      ccacacatc ctcaagcaa caattcacc gacttggac 
      ttgcaaagt aaaaaagtg gcggcgcaa atttcctac 
      gccactctc gccgaaaac cggcggaaa aacagatac 
      acgaatatt ctgcctttc gacgaaaca cgtgttaaa 
      ttaaatata gacgaagac gatgaaatt agctcggat 
      tacatcaac gcctcgtac ataaagggt tattcgggt 
      aaaatggaa tacatcgcc actcagggc cccctagaa 
      tccacaacc cgcgatttc tggaaaatg gtcttacag 
      gaaaacgtt tcaataatc gtgatggtg tcacaattc 
      gtcgaacaa agtaaagag aaatgttac cattatttc 
      cccagtaat cacgaaagc gtcatgttt ggggaatca 
      ctggaagtt aagtgttgt actgaactg catttcggg 
      acttattgt atgcgaact ttgcaagtg cgaaagggc 
      acagagcag cgacatgtc attcacatg caatttctg 
      gaatggcct gactttggc gttccgtta ggaaccgac 
      aacatgtta caattttgc cgacagttg cgggaaaaa 
      gcaaatagt gaaggaggt ctaattgtg gtgcattgc 
      agcgctggc gttggtagg actgggacg ttgatagct 
      ctagacata cttcttcag accattgat gatcacaga 
      gacattaat atttataag actgttctt gacctaaga 
      aaacaaaga gcaaacatg gtacaaacc gaaaaacaa 
      tacgtattc atccacact tgtgtgaac gactatttg 
      gaaacaccc ataattcaa tcaaacgac tcgtcacaa 
      gagcgaata tatgaaaac atggacgtg ttgaaatat 
      tccctgaag aaaaatcta attgagact gaggtcgaa 
      agcgcctta tag
    mRNA sequence
    • >TC007325 RA
      cttatcgtc ccgcagtgc cttaaacag ccgtagtct 
      tccacacgt ttcccaaat agcgcttaa ttaacgtca 
      tgtacttaa taaaatatt cgctggttg tcagttcag 
      agataatag caaatatcg aagttttat cattttcgg 
      taagcccgc aaattgtgt ttgcttagt gctccataa 
      tccaattgt tcatcaaac gcaatcttg ctatctcaa 
      aaagttata aatttcgag tacctgcgt aacaaatcg 
      agagtgttt aggaaaatg ttaactcta taaaattta 
      ttcattact catacgtgt ggtgctgaa cggtcgtgt 
      tgataaagg catcctgtt taacactgc gaatatata 
      tttaagctg atgtgacag aaaattgaa taaaaaatg 
      aacgtggtg tgggttctg gtgcttcta gtgccgagt 
      gttttcgca aaattgaca aacatcgag acgcaaact 
      ggccaaaac cacgaaacg aacgaaatt gaagatgtt 
      tttgtgaag caagaaaca acttcaggg cctgaaaat 
      gaagcttca actactgaa atatattca actactaca 
      gagcctaca tttttttca aaaacggct tgcgctaac 
      acttctgag gaacaaagt gtcacaaca gaagcacac 
      attcctgtg gttaatttt acacaaatc aatgcctca 
      agttttgaa gtgtttcta caatgggat ccccccgaa 
      aacacacag tgtgtcgac aaatacgta atttacctc 
      gacagtgat ttttacgaa accaccgaa agtaattac 
      actatcgct aatttaaca gcttgtaat gaatacaaa 
      gttgcaatt gcggcttcg aagaatgac aacgtggga 
      caatttaca aacgatagt tttaagaca attgctccc 
      aatccgttc ggttacgga gttgaggac ttgaaagtt 
      gagcaagtc accgattac gttttaaat ctgacatgg 
      aatgcacca gatgaaaag gtcgtgcaa tgtgtaacc 
      gcttacgat atactttgg tggcctgcc ggcagtacg 
      ggcaaaacc aaagagagt aaaaccaac gaaacgtat 
      ttagaatta tcgccagtt gttggttgt atgaaatat 
      gaaataaat gtgaagccc ttaattgga gaaacccaa 
      caaggaaat gtccaagcg gcggaattt caagctcaa 
      gcctatcca aacaatcct ccaaatatt gtcatcggc 
      tcattatca agcaccaaa acacaaata agcctaact 
      tgggaaacc gacgataaa tcgaagaat atgtgcaat 
      gtgagtgca attgtggta aattgtgtt gcaccagaa 
      gattcaaac agaaatagt tcctggata atctgggag 
      gcacaaagt gtgagagaa gtgaccgaa caaaccgcc 
      aataaccga tactcaatg acaatcgag aatttgtca 
      ccttttacg aactattca tgttcggga gttgtcaca 
      aacactggc ggaaattcg tctgaaacc ccgtttgaa 
      gctacgacc gatgaagac gttccttcc gctccacag 
      tcactcaca attaaagat gtggggccc caaaaattc 
      acaatcgaa tggacacaa cccgaacaa atccccgga 
      attatgact tcgtatggt ttgaaaatc attagtgtc 
      ggacctctt gaccggaat gtgaataaa gactgtgcg 
      aacaatgcc actgatcac ttttttgac ctcgatgct 
      gaagatact agtcttgtt tttgaggaa gctttgccg 
      tattttaaa tacagtgtg tctgtctat ggagtcgat 
      ggggccgga aatgggaca gaagcgaat gcagacgcg 
      agcacaaca gccacaaag ccggatcaa gttgaaaac 
      gcggaaact ttaccaccg gaatattcc caaaacgaa 
      acttactcc ggctctgtg atcgtcaaa ttcgaccct 
      ccatgcaac acaaacgga cctttcaaa cattatgaa 
      ataaattat atcggacag agagaaggt ttcagtcct 
      gaaaaccaa acacttgaa accactgaa acaaacgca 
      acaattcca ctaaaacct gaacgcgaa tattcgatt 
      gaaattaaa gttgtgact gaaaatttt acttcaggc 
      ccggtttca gtgccggcg tttcagtcg caagctggt 
      gtacctgtg gttaacaaa aatgtggac ttgaaagca 
      ccaactcct gaagaagaa aaggcacaa ttcgcgttc 
      aaaaaagaa aattttgat aacataaac ggcgagatt 
      attgcttat tgtttgata cttttgacg gaatcacaa 
      gaagggggt tacggtttt tgggacggg acaaacgaa 
      acatggcct ccggtaccc aaaaatggc actcaatta 
      acaccaaac ttctggaac cctttcaaa gacggagtg 
      agctcggct aatttcaca attggcgat ggtggttgt 
      ggtgccaac tattgcaac aaaatccta aatgacgac 
      acaaaatat tggttgatt gtgcgagcc ctaacaaga 
      gcgggtttt aaggacagt tcattatta ccttttaaa 
      acagatgtg atggaagaa ttgaatttg cccctaatt 
      ctgggctta acattcggt ctcttactt ttagtactt 
      ttgataatt ttcttgata attttgtgg cgacgcagt 
      cggaaaagt cagccaccg gaagaaata aaacgagtt 
      tcaacaaca agccctgaa cccatcccc tgccaaaaa 
      ttcgtcact ttttacgct aatctcaaa acgactcca 
      cacatcctc aagcaacaa ttcaccgac ttggacttg 
      caaagtaaa aaagtggcg gcgcaaatt tcctacgcc 
      actctcgcc gaaaaccgg cggaaaaac agatacacg 
      aatattctg cctttcgac gaaacacgt gttaaatta 
      aatatagac gaagacgat gaaattagc tcggattac 
      atcaacgcc tcgtacata aagggttat tcgggtaaa 
      atggaatac atcgccact cagggcccc ctagaatcc 
      acaacccgc gatttctgg aaaatggtc ttacaggaa 
      aacgtttca ataatcgtg atggtgtca caattcgtc 
      gaacaaagt aaagagaaa tgttaccat tatttcccc 
      agtaatcac gaaagcgtc atgtttggg gaatcactg 
      gaagttaag tgttgtact gaactgcat ttcgggact 
      tattgtatg cgaactttg caagtgcga aagggcaca 
      gagcagcga catgtcatt cacatgcaa tttctggaa 
      tggcctgac tttggcgtt ccgttagga accgacaac 
      atgttacaa ttttgccga cagttgcgg gaaaaagca 
      aatagtgaa ggaggtcta attgtggtg cattgcagc 
      gctggcgtt ggtaggact gggacgttg atagctcta 
      gacatactt cttcagacc attgatgat cacagagac 
      attaatatt tataagact gttcttgac ctaagaaaa 
      caaagagca aacatggta caaaccgaa aaacaatac 
      gtattcatc cacacttgt gtgaacgac tatttggaa 
      acacccata attcaatca aacgactcg tcacaagag 
      cgaatatat gaaaacatg gacgtgttg aaatattcc 
      ctgaagaaa aatctaatt gagactgag gtcgaaagc 
      gccttatag ccaatttta attcaattg tactta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY