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Gene information
General information
TC011530 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3653106..3655759
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011530
    iBeetle screen iB_09907

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011530 RA
      MIDEEAQYD ALLGGSEEL PSNISLSKF VACRSQEIQ 
      IMKNSLLTQ TGSKLAFQT LPKHMRRRA MSHNVKRLP 
      RRLRQIHLN QMKKSGMPT QQKRPSRKY RRKPRNLLE 
      EYTKRANKK YKWLQTHIW HAKRFHMVE KWGYKLPYH 
      ACDKAFRAC YRAMTQHCL LQDISYYSC VEIHGDKSE 
      IIEKIKLLC KPGEGLSIG AKAFSEGNR EGEICLYDS 
      FQKAIGNVM FQWQPSIDE KCKLWLWVH AAFYSTTKN 
      TLVECFKNC QIEINELRL ELSRFRLSG PLSNPVLQD 
      ALALIDIYD IKLVPESPL KKYIDWPEN KAVIINQCE 
      FWKKLASVA SLHEVSPHI VLPLIVKDP RLNLPKKRI 
      KSVLTFGKL EFDQIPVNI SANPLWDKT IRTYSNENK 
      VSDSKIVDL QSGLLVPGS DLKGTIGHI PIILIQRPG 
      NKTQNVGLG SGWDVILPT AWAKPFWIA FVMRGARVG 
      GLRESDSML YEMGKSQLL HPDTDAGFE EELRHSNQH 
      RVTFFQKPP NKRINYSKF KIASPLLCN WRLLLKDWN 
      SGFTGEFSV LRDKKQLHS INAFLNSKT HPVPQCPQN 
      CLIPVRLEC EKRGSLAEY AIICVPKCK SDLSEVLLE 
      PNCEDVNKE KRKELRRAH QSLLKKLRK KRKLKSELA 
      SDDHSEILR SYTEKMRAL WLPEPTTIR HSCSREVVG 
      FVSKGGFSF SIGKSAGTG YITSDCLSE LVDFRNKVL 
      LRNTNSRQY KTANLSVIV E
    CDS sequence
    • >TC011530 RA
      atgattgac gaagaagcc caatatgac gcccttctg 
      gggggctcc gaggagctc ccttcgaac atctcctta 
      tcgaaattc gtggcttgc cgcagccaa gaaatccaa 
      atcatgaaa aactccctc ctgacccaa accggctca 
      aaactggcc ttccaaact ctaccgaaa cacatgaga 
      cgccgggca atgtcccac aacgtcaaa agactacca 
      cggcgtctc cgccaaatc catttaaac caaatgaaa 
      aaatccgga atgccaacg caacagaag cgcccctct 
      cgcaaatac cgccgaaaa ccgcgaaac ctcctcgaa 
      gaatacacc aaaagggcc aataagaag tacaaatgg 
      ttgcaaacc cacatctgg cacgcgaaa cggttccac 
      atggtggag aaatggggc tacaagttg ccataccac 
      gcttgtgat aaggccttc cgtgcctgc tacagggca 
      atgacccaa cattgcctc ttacaggac atttcctac 
      tacagttgt gtggaaatc cacggcgat aaaagcgaa 
      attattgaa aaaattaaa ctcctgtgt aagccaggc 
      gagggttta agtattggg gcgaaagcc ttttctgag 
      ggtaaccgc gagggtgaa atctgtctc tatgattcg 
      ttccaaaaa gccatcggg aacgtcatg ttccagtgg 
      caaccctct attgacgaa aaatgtaaa ttgtggttg 
      tgggttcat gccgctttt tattcaacc acgaaaaac 
      acactagtt gagtgtttt aaaaactgc cagatcgaa 
      ataaacgaa cttagactt gagttgagc agatttaga 
      ctcagtggg cctttatct aatccagtt cttcaagat 
      gcgcttgct ttgatcgac atttatgat attaaatta 
      gtaccagag tcgcctctt aaaaaatac attgattgg 
      ccggagaat aaagcagtg attataaat cagtgcgag 
      ttttggaaa aaactagcc tcggtggca tccctacat 
      gaggtttcc ccccacatc gtgttaccc ttgattgtg 
      aaagacccc cgattgaat cttccaaaa aagcgcata 
      aaaagtgtt ttaactttc gggaaattg gaatttgat 
      caaattcct gtgaacatt tccgcgaat cctttatgg 
      gacaagacc atcagaacg tactcaaat gaaaataaa 
      gtgtctgac tcaaaaatt gttgacttg caaagtggt 
      ttactagtc ccggggtct gatttaaaa gggaccatt 
      ggccacatt ccaattatc ctgatccaa aggccaggg 
      aataaaacc cagaacgtt ggtttaggg agtggatgg 
      gatgtcatc ctaccaacg gcttgggcc aaaccgttt 
      tggattgcc ttcgttatg aggggagca agggttggg 
      ggtttgagg gaatcagac tcaatgctt tacgaaatg 
      ggaaagtca cagttgctt cacccggac acggacgca 
      ggttttgag gaagaactg agacattca aaccagcac 
      agggttacg tttttccaa aaacctcca aataaacga 
      atcaattac agtaaattt aaaatcgca agtcctttg 
      ctttgcaat tggaggctt ttgctcaag gactggaac 
      agtggcttt acgggcgaa ttttcagtc ctcagggac 
      aaaaaacag cttcattcg attaatgct tttctcaat 
      tctaagaca cacccggtg ccacagtgc ccccaaaat 
      tgcctcatt cctgttaga cttgagtgt gagaagcgg 
      ggctcactg gccgagtat gccattatt tgtgtcccg 
      aaatgtaag agtgatttg agcgaagtt ttgttggaa 
      ccgaattgc gaggatgta aataaggaa aagcggaag 
      gaattgaga cgtgcacat caaagtttg ttgaaaaaa 
      ttgcggaag aaacgcaaa ttaaagtcg gagcttgcg 
      tcggatgat cacagtgag attttacgc agttatacg 
      gagaaaatg agagcgctg tggcttcct gagccaact 
      accataagg cattcgtgt agcagagaa gttgtaggt 
      tttgtttcg aaaggcggt tttagtttt tcgattggg 
      aaaagcgca gggacgggt tatataact agcgattgt 
      ttatcggag ttggttgat tttagaaat aaggttttg 
      ttgaggaat actaattct cggcaatat aaaactgcg 
      aatttaagc gtcattgta gagtga
    mRNA sequence
    • >TC011530 RA
      taaaactag caaaggtat ttttttgtt ttaaagcta 
      cgcggtaaa tttgaatga ttctctcaa tttttacat 
      taaaatgtg ataagtgcg aatttactt tcaataata 
      ttcattgct agcttcagt aattagcgc ctggaagcg 
      tcaaaacca tgattgacg aagaagccc aatatgacg 
      cccttctgg ggggctccg aggagctcc cttcgaaca 
      tctccttat cgaaattcg tggcttgcc gcagccaag 
      aaatccaaa tcatgaaaa actccctcc tgacccaaa 
      ccggctcaa aactggcct tccaaactc taccgaaac 
      acatgagac gccgggcaa tgtcccaca acgtcaaaa 
      gactaccac ggcgtctcc gccaaatcc atttaaacc 
      aaatgaaaa aatccggaa tgccaacgc aacagaagc 
      gcccctctc gcaaatacc gccgaaaac cgcgaaacc 
      tcctcgaag aatacacca aaagggcca ataagaagt 
      acaaatggt tgcaaaccc acatctggc acgcgaaac 
      ggttccaca tggtggaga aatggggct acaagttgc 
      cataccacg cttgtgata aggccttcc gtgcctgct 
      acagggcaa tgacccaac attgcctct tacaggaca 
      tttcctact acagttgtg tggaaatcc acggcgata 
      aaagcgaaa ttattgaaa aaattaaac tcctgtgta 
      agccaggcg agggtttaa gtattgggg cgaaagcct 
      tttctgagg gtaaccgcg agggtgaaa tctgtctct 
      atgattcgt tccaaaaag ccatcggga acgtcatgt 
      tccagtggc aaccctcta ttgacgaaa aatgtaaat 
      tgtggttgt gggttcatg ccgcttttt attcaacca 
      cgaaaaaca cactagttg agtgtttta aaaactgcc 
      agatcgaaa taaacgaac ttagacttg agttgagca 
      gatttagac tcagtgggc ctttatcta atccagttc 
      ttcaagatg cgcttgctt tgatcgaca tttatgata 
      ttaaattag taccagagt cgcctctta aaaaataca 
      ttgattggc cggagaata aagcagtga ttataaatc 
      agtgcgagt tttggaaaa aactagcct cggtggcat 
      ccctacatg aggtttccc cccacatcg tgttaccct 
      tgattgtga aagaccccc gattgaatc ttccaaaaa 
      agcgcataa aaagtgttt taactttcg ggaaattgg 
      aatttgatc aaattcctg tgaacattt ccgcgaatc 
      ctttatggg acaagacca tcagaacgt actcaaatg 
      aaaataaag tgtctgact caaaaattg ttgacttgc 
      aaagtggtt tactagtcc cggggtctg atttaaaag 
      ggaccattg gccacattc caattatcc tgatccaaa 
      ggccaggga ataaaaccc agaacgttg gtttaggga 
      gtggatggg atgtcatcc taccaacgg cttgggcca 
      aaccgtttt ggattgcct tcgttatga ggggagcaa 
      gggttgggg gtttgaggg aatcagact caatgcttt 
      acgaaatgg gaaagtcac agttgcttc acccggaca 
      cggacgcag gttttgagg aagaactga gacattcaa 
      accagcaca gggttacgt ttttccaaa aacctccaa 
      ataaacgaa tcaattaca gtaaattta aaatcgcaa 
      gtcctttgc tttgcaatt ggaggcttt tgctcaagg 
      actggaaca gtggcttta cgggcgaat tttcagtcc 
      tcagggaca aaaaacagc ttcattcga ttaatgctt 
      ttctcaatt ctaagacac acccggtgc cacagtgcc 
      cccaaaatt gcctcattc ctgttagac ttgagtgtg 
      agaagcggg gctcactgg ccgagtatg ccattattt 
      gtgtcccga aatgtaaga gtgatttga gcgaagttt 
      tgttggaac cgaattgcg aggatgtaa ataaggaaa 
      agcggaagg aattgagac gtgcacatc aaagtttgt 
      tgaaaaaat tgcggaaga aacgcaaat taaagtcgg 
      agcttgcgt cggatgatc acagtgaga ttttacgca 
      gttatacgg agaaaatga gagcgctgt ggcttcctg 
      agccaacta ccataaggc attcgtgta gcagagaag 
      ttgtaggtt ttgtttcga aaggcggtt ttagttttt 
      cgattggga aaagcgcag ggacgggtt atataacta 
      gcgattgtt tatcggagt tggttgatt ttagaaata 
      aggttttgt tgaggaata ctaattctc ggcaatata 
      aaactgcga atttaagcg tcattgtag agtgactgg 
      ctgaatata aatttatta caggaaaac gtttataat 
      tgctattta caaatacat acccacatg gggaggctg 
      caaatgccg aatttttga ataaaaaat aatgcaatt 
      t
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