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Gene information
General information
TC008166 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5995908..5997446
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008166
    iBeetle screen iB_09902

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008166 RA
      MSNFKRLLN VPHVNKTTF RLSHKSHLE ALRERIING 
      PSLQDFIKS PDPVLKEDS EAWKDYNGR LKRTKSESS 
      RLRLPPWLK TSIPKGKKF SELKEQLRN LNLHTVCEE 
      ARCPNIGEC WGGGEHGTA TATIMLLGD TCTRGCRFC 
      SVKTSKTPP APDPNEPVK TASAIASWG LDYIVLTSV 
      DRDDLPDGG SNHFAETVR EIKKRKSEI LVECLVPDF 
      AGDLNAVKT IVDSGLDVY AHNIETVEA LTPFVRDRR 
      AKYRQSLAT LEAVKKFNP DMITKSSIM LGLGETDDE 
      VRQTLEDLK TAGVDCVTL GQYMQPTKK HLLVVEYVT 
      PEKYQYWEK VGNEMKFLY VASGPLVRS SYKAGEFFI 
      ASILKNRKT NAV
    CDS sequence
    • >TC008166 RA
      atgtccaac tttaaacgc ctcttaaat gtgccccac 
      gttaacaaa accactttt cgattatcc cacaaatca 
      cacttggaa gccctccga gaacggatt ataaacggc 
      cccagtctc caagacttt ataaagagc cccgatcct 
      gtcttaaaa gaagactcc gaggcttgg aaagactac 
      aatgggagg ctcaaaaga accaaaagt gaaagttcc 
      cgtcttagg ttaccccca tggttaaag acctcaatt 
      cccaaagga aaaaaattc agcgagctt aaagaacag 
      ttaaggaac cttaatttg cacactgtg tgcgaggag 
      gcaaggtgt ccaaatatt ggggagtgt tggggtgga 
      ggcgagcat gggacggct acagctacc atcatgttg 
      ttaggagac acttgtaca agggggtgc cgattctgt 
      tccgttaag acctcaaaa acgcctcca gcacccgac 
      ccaaacgaa cctgttaaa actgcctca gctatagca 
      tcatggggg ctggactat attgtgcta acatcagtt 
      gaccgcgac gatttgcct gatggaggt tcaaatcat 
      tttgccgaa actgtgaga gagataaag aagcgtaag 
      agcgagatt ctagtagag tgcttagtg cctgacttt 
      gctggtgac ctaaatgct gtgaagaca attgttgat 
      tcaggtttg gatgtttac gctcacaat attgaaact 
      gttgaagct ttaactccg tttgtaaga gacagaaga 
      gcaaaatat aggcagtcg ttggcaact cttgaagct 
      gttaaaaaa tttaacccc gatatgatt acaaagtcg 
      tcaattatg ttgggtttg ggtgaaact gacgatgaa 
      gttagacaa actttggag gatttaaag acagcaggt 
      gttgattgt gtgactctt ggacagtac atgcaacca 
      actaaaaaa catctactg gttgttgaa tatgtaaca 
      cctgagaag tatcaatat tgggaaaaa gtaggaaat 
      gaaatgaag tttttgtac gttgcgagt ggacctctt 
      gtgagaagt tcctacaaa gctggtgaa ttcttcatt 
      gcgagtatt ttaaaaaat agaaaaact aatgctgtg 
      taa
    mRNA sequence
    • >TC008166 RA
      ttatggtga aaagtaaac atctttaga ggttagatc 
      aaaatcact gatgacctt ttaacctca atcatttcg 
      caaaaatgt ccaacttta aacgcctct taaatgtgc 
      cccacgtta acaaaacca cttttcgat tatcccaca 
      aatcacact tggaagccc tccgagaac ggattataa 
      acggcccca gtctccaag actttataa agagccccg 
      atcctgtct taaaagaag actccgagg cttggaaag 
      actacaatg ggaggctca aaagaacca aaagtgaaa 
      gttcccgtc ttaggttac ccccatggt taaagacct 
      caattccca aaggaaaaa aattcagcg agcttaaag 
      aacagttaa ggaacctta atttgcaca ctgtgtgcg 
      aggaggcaa ggtgtccaa atattgggg agtgttggg 
      gtggaggcg agcatggga cggctacag ctaccatca 
      tgttgttag gagacactt gtacaaggg ggtgccgat 
      tctgttccg ttaagacct caaaaacgc ctccagcac 
      ccgacccaa acgaacctg ttaaaactg cctcagcta 
      tagcatcat gggggctgg actatattg tgctaacat 
      cagttgacc gcgacgatt tgcctgatg gaggttcaa 
      atcattttg ccgaaactg tgagagaga taaagaagc 
      gtaagagcg agattctag tagagtgct tagtgcctg 
      actttgctg gtgacctaa atgctgtga agacaattg 
      ttgattcag gtttggatg tttacgctc acaatattg 
      aaactgttg aagctttaa ctccgtttg taagagaca 
      gaagagcaa aatataggc agtcgttgg caactcttg 
      aagctgtta aaaaattta accccgata tgattacaa 
      agtcgtcaa ttatgttgg gtttgggtg aaactgacg 
      atgaagtta gacaaactt tggaggatt taaagacag 
      caggtgttg attgtgtga ctcttggac agtacatgc 
      aaccaacta aaaaacatc tactggttg ttgaatatg 
      taacacctg agaagtatc aatattggg aaaaagtag 
      gaaatgaaa tgaagtttt tgtacgttg cgagtggac 
      ctcttgtga gaagttcct acaaagctg gtgaattct 
      tcattgcga gtattttaa aaaatagaa aaactaatg 
      ctgtgtaat taattgttg ttaaataaa aagtatatt 
      gacgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY