Status
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Gene information
General information
TC007351 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5803087..5805756
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007351
    iBeetle screen iB_09901

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007351 RA
      MLKRLLILL FCFECFVWC CIREPSIYH SKAPKLPGD 
      NGFQIRINE NPEKFIPGK VYTLYLTHN KLQHFKRFT 
      INVEAASEP GNLSPQRVG SFQLFSDSI SKFNEECVN 
      TISESNDSP KTEAFFMWA APPPGSGCV TFKAMVLQD 
      STHWYADEG NLTKTFCEQ TEKDMKFDE SDCCACDEA 
      KYSMIFEGI WSNKTHPKD FPFSLWLTH FSDVIGASH 
      EKNFSFWGE GQIASEGFR SLAEWGSVR LMETELRAK 
      SKYLRTIIK AAGLWYPNV NTNTSANFR VDRRHHLIS 
      LSSMLGPSP DWVVGVNGL NLCLKNCTW IENLIIDLF 
      PYDAGTDSG ISYMSPNAE TKPRERMYR ITTTYPEDP 
      RAPFYDPSK KEMTPLARL YLKREKLIP KNCDENFLN 
      AQLDVSENT EEINRPECA VTEYSQWSE CSVTCGKGL 
      RMRSREYRM PQKAKMFNC NRQLISKEM CVAAVPECE 
      GGTPEEEES IEDIPLENI EGICATSPW GPWSECSAT 
      CGVGFKMRT RLFIDSMGR KKCPHITVV EKEKCMSPP 
      CTADQIEIP DPMCPTTDW SDWSPCSAS CGKGIRMRT 
      RLLLVEPGL QQKCSSRVE LIQQAPCMD TPDCTIDMA 
      TAKVICMQE SEPGPCTGY FNRWYFEPR KMMCVPFIY 
      GGCRGNRNN FLTAQECME ACQVVRDAL KGNINNNNN 
      ENANSNPSA VASSNGRFV DCMVSDWSP WSQCSVTCG 
      TGISEKFRM VKRPAENGG RPCPSRLVK KRRCRGPPC 
      V
    CDS sequence
    • >TC007351 RA
      atgttaaaa cgactgctg atcctcctg ttttgtttc 
      gagtgtttt gtttggtgt tgcattcgt gagcccagc 
      atctaccac tcaaaagcg cccaaactg ccgggtgat 
      aatggcttc caaatccga attaacgaa aaccccgaa 
      aaatttatc ccgggcaaa gtttacacc ctttattta 
      acccacaac aaattacaa cacttcaaa cgcttcaca 
      attaacgtg gaagccgcc tccgagcct ggcaaccta 
      tcgccgcaa cgagtcggc agcttccag ctgtttagc 
      gactcgatt tctaaattt aacgaggaa tgtgtaaat 
      acaatttcc gaaagtaat gattcgcca aaaaccgag 
      gcctttttc atgtgggcc gctccgcct ccaggctcg 
      ggctgcgtc actttcaag gccatggtc ctgcaggac 
      tcgacccac tggtacgcc gatgagggc aacctgacc 
      aaaaccttc tgcgagcag acggaaaaa gatatgaaa 
      tttgacgaa agcgactgt tgtgcttgt gacgaggcc 
      aaatatagc atgattttt gaaggcatt tggtccaat 
      aaaacacac cccaaggat tttcctttt tccttgtgg 
      ctgactcac ttttccgat gtgatcggc gcttcgcac 
      gagaagaat tttagtttc tggggcgaa ggccagatc 
      gcctctgaa gggttcagg agcctggcc gaatggggg 
      tctgtgaga ctcatggag accgaatta agggccaaa 
      agtaaatac ttgaggact attattaaa gcagctggg 
      ctgtggtac cccaacgtc aatactaac acttcggcc 
      aattttcga gtcgacagg agacaccat ttgatttct 
      ttaagctca atgcttggg ccttccccg gactgggtt 
      gtcggagtt aacggtttg aacctttgt ctcaaaaac 
      tgtacctgg attgaaaat ttgatcatt gatctattt 
      ccatacgat gctggaacc gacagtgga atctcttac 
      atgtccccg aacgctgag actaaaccg agggaacgc 
      atgtaccga atcactacg acttaccct gaggatcca 
      agagccccg ttttacgac ccttcgaaa aaagaaatg 
      acacctttg gctcggctt tacttgaaa cgcgaaaaa 
      ttaattccg aaaaattgc gacgaaaac ttcctaaac 
      gcccaactt gacgttagc gaaaacacg gaagaaatc 
      aacagaccc gaatgtgcc gtcaccgag tacagccaa 
      tggagcgaa tgttccgtc acttgtggc aaaggcttg 
      cggatgaga agtcgcgag tatcgaatg ccccaaaaa 
      gccaaaatg ttcaactgc aaccggcag ctgatttcg 
      aaagaaatg tgcgtggct gcagtcccc gaatgcgag 
      ggcggaacc cccgaagaa gaagaatca atcgaggac 
      attccctta gaaaacatc gaagggatc tgcgccaca 
      tccccttgg ggcccttgg tccgaatgt tccgccacg 
      tgtggcgtt ggttttaaa atgcgtacc cgcctattt 
      atcgatagt atgggccgt aaaaagtgc ccacatatc 
      actgttgtg gaaaaggag aagtgtatg agtccccct 
      tgtactgcc gaccagatt gaaattccc gatcccatg 
      tgccccaca accgactgg agtgactgg agtccttgt 
      agcgcttcc tgtggtaaa ggaatcagg atgcggacg 
      cgtttgcta ttggtagag ccgggatta cagcagaaa 
      tgtagctca agagtggag ttaatacaa caagcacct 
      tgtatggac actcctgac tgtactata gacatggca 
      acggccaaa gttatttgc atgcaagaa agcgagcct 
      ggaccgtgc actggttac ttcaaccgt tggtatttc 
      gagccccgc aaaatgatg tgtgttccg ttcatttat 
      gggggctgt aggggcaac aggaataat ttcttgacg 
      gcgcaggaa tgtatggaa gcgtgtcag gtagttcga 
      gatgctttg aagggtaat attaataat aataataac 
      gaaaatgca aattcgaat ccttcagcc gttgcgtcc 
      agcaatggc agatttgtt gattgcatg gtttcggat 
      tggtcccct tggtcccaa tgtagcgta acgtgtggg 
      accggaatt tccgaaaag ttcagaatg gtcaaaaga 
      ccagccgag aacgggggg cgcccttgc ccctcgaga 
      ctcgtcaag aagcggcga tgtcggggg ccgccatgt 
      gtctaa
    mRNA sequence
    • >TC007351 RA
      agtcactgc caatatgtt aaaacgact gctgatcct 
      cctgttttg tttcgagtg ttttgtttg gtgttgcat 
      tcgtgagcc cagcatcta ccactcaaa agcgcccaa 
      actgccggg tgataatgg cttccaaat ccgaattaa 
      cgaaaaccc cgaaaaatt tatcccggg caaagttta 
      cacccttta tttaaccca caacaaatt acaacactt 
      caaacgctt cacaattaa cgtggaagc cgcctccga 
      gcctggcaa cctatcgcc gcaacgagt cggcagctt 
      ccagctgtt tagcgactc gatttctaa atttaacga 
      ggaatgtgt aaatacaat ttccgaaag taatgattc 
      gccaaaaac cgaggcctt tttcatgtg ggccgctcc 
      gcctccagg ctcgggctg cgtcacttt caaggccat 
      ggtcctgca ggactcgac ccactggta cgccgatga 
      gggcaacct gaccaaaac cttctgcga gcagacgga 
      aaaagatat gaaatttga cgaaagcga ctgttgtgc 
      ttgtgacga ggccaaata tagcatgat ttttgaagg 
      catttggtc caataaaac acaccccaa ggattttcc 
      tttttcctt gtggctgac tcacttttc cgatgtgat 
      cggcgcttc gcacgagaa gaattttag tttctgggg 
      cgaaggcca gatcgcctc tgaagggtt caggagcct 
      ggccgaatg ggggtctgt gagactcat ggagaccga 
      attaagggc caaaagtaa atacttgag gactattat 
      taaagcagc tgggctgtg gtaccccaa cgtcaatac 
      taacacttc ggccaattt tcgagtcga caggagaca 
      ccatttgat ttctttaag ctcaatgct tgggccttc 
      cccggactg ggttgtcgg agttaacgg tttgaacct 
      ttgtctcaa aaactgtac ctggattga aaatttgat 
      cattgatct atttccata cgatgctgg aaccgacag 
      tggaatctc ttacatgtc cccgaacgc tgagactaa 
      accgaggga acgcatgta ccgaatcac tacgactta 
      ccctgagga tccaagagc cccgtttta cgacccttc 
      gaaaaaaga aatgacacc tttggctcg gctttactt 
      gaaacgcga aaaattaat tccgaaaaa ttgcgacga 
      aaacttcct aaacgccca acttgacgt tagcgaaaa 
      cacggaaga aatcaacag acccgaatg tgccgtcac 
      cgagtacag ccaatggag cgaatgttc cgtcacttg 
      tggcaaagg cttgcggat gagaagtcg cgagtatcg 
      aatgcccca aaaagccaa aatgttcaa ctgcaaccg 
      gcagctgat ttcgaaaga aatgtgcgt ggctgcagt 
      ccccgaatg cgagggcgg aacccccga agaagaaga 
      atcaatcga ggacattcc cttagaaaa catcgaagg 
      gatctgcgc cacatcccc ttggggccc ttggtccga 
      atgttccgc cacgtgtgg cgttggttt taaaatgcg 
      tacccgcct atttatcga tagtatggg ccgtaaaaa 
      gtgcccaca tatcactgt tgtggaaaa ggagaagtg 
      tatgagtcc cccttgtac tgccgacca gattgaaat 
      tcccgatcc catgtgccc cacaaccga ctggagtga 
      ctggagtcc ttgtagcgc ttcctgtgg taaaggaat 
      caggatgcg gacgcgttt gctattggt agagccggg 
      attacagca gaaatgtag ctcaagagt ggagttaat 
      acaacaagc accttgtat ggacactcc tgactgtac 
      tatagacat ggcaacggc caaagttat ttgcatgca 
      agaaagcga gcctggacc gtgcactgg ttacttcaa 
      ccgttggta tttcgagcc ccgcaaaat gatgtgtgt 
      tccgttcat ttatggggg ctgtagggg caacaggaa 
      taatttctt gacggcgca ggaatgtat ggaagcgtg 
      tcaggtagt tcgagatgc tttgaaggg taatattaa 
      taataataa taacgaaaa tgcaaattc gaatccttc 
      agccgttgc gtccagcaa tggcagatt tgttgattg 
      catggtttc ggattggtc cccttggtc ccaatgtag 
      cgtaacgtg tgggaccgg aatttccga aaagttcag 
      aatggtcaa aagaccagc cgagaacgg ggggcgccc 
      ttgcccctc gagactcgt caagaagcg gcgatgtcg 
      ggggccgcc atgtgtcta acaatcagt aacagttgc 
      cagttatgt acttgttta gagcaataa acaatttaa 
      agaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY