Status
Please wait ...
Gene information
General information
TC007375 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5373362..5375394
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007375
    iBeetle screen iB_09899

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007375 RA
      MEDKRFAHI KTDPKFRRI PKGARKVKI DKRFQSMFK 
      DKKFKVKYT VDKRGRPVN HTSTEDLKR YYELSSSES 
      SSDEEPEEP GKYVKAPQN DLNVKDKSD KELPSDVKK 
      KLRDLNVDY ARGEGNLLS ESSSDDEET DEEVSEGEQ 
      IEHKWGELD NDADRTDEA TYRLAACNL DWDRIRAVD 
      LMVLFNSFL PPGGAVKSV VIYPSEFGK ERMKEEEVK 
      GPIELVEHK EIEEEDTEE GSKYHMEKL RQYQLNRLK 
      YYYAVVTFD GANTANKVY TECDGMEYE SSATKLDLR 
      FIPDDMTFD DEPKEQCDK LPELNKYQP RFFTTTALQ 
      QAKVDLTWD ETDPDRIEM TQKLNTGKI EEIPEHELQ 
      NFVAVSDEE SEDEEKNAT SDDDESTNN PIDKYKQLL 
      QEIEEKEQE KKNREVEME VSWGIDLKD KTEKLVKKK 
      IENEEEKTP FQKYLDKRK EKRKEKKKK KRENEDSDD 
      VPSDIDMND PYFAEEFNN PEFKKKKVK EKKSQSEEE 
      NNPELELLL ADEDDDNKK HFSLKKIQK QEEATSKKK 
      RKLKETDEK DDFQVDVND DRFSALFTS HLYNIDPTD 
      PHYKKTKGM ERLINEKIK RRKDDSERE MTEKPMKKD 
      AELSVLVKS VKRKAEALS NKKK
    CDS sequence
    • >TC007375 RA
      atggaagat aaacgcttc gcgcacata aaaaccgac 
      cccaaattc cggcggatc cccaaaggg gcccgcaag 
      gttaaaatc gacaagcgg ttccagtcc atgttcaaa 
      gacaagaag ttcaaagtg aaatacacg gtggacaag 
      cgtgggaga cccgtaaat cacacatcg actgaagat 
      ctgaagcgt tattacgaa ctgagttcg agtgagagt 
      tcctcagat gaagaacca gaagagccc ggcaagtac 
      gtcaaagcg ccccaaaac gacctaaat gtgaaagat 
      aaaagcgat aaggagctt cccagcgac gtgaagaag 
      aaacttcgt gacttgaat gtcgactac gcccgaggg 
      gagggaaat ttattgagt gagagttct tcagatgac 
      gaggaaacg gatgaggaa gtgtctgaa ggggagcag 
      attgagcac aaatgggga gagttggat aatgacgcc 
      gataggaca gatgaagcc acgtatcgt ttggcggct 
      tgtaacctg gactgggat aggataagg gcggtagat 
      ttgatggtc ctgttcaat tcgtttttg ccccccggt 
      ggggctgtt aagtcagtt gtgatttat ccctcggag 
      tttggcaaa gaacgaatg aaggaagag gaggtgaaa 
      gggccaatt gaattggtg gaacacaag gaaattgag 
      gaagaagac acggaagaa ggctcaaaa tatcacatg 
      gaaaaattg aggcagtac caactcaac cgactgaag 
      tattattac gctgttgtg acctttgac ggggctaac 
      actgcaaat aaagtgtac acagagtgt gacgggatg 
      gagtatgaa tcaagcgcg acaaagctt gatttacgc 
      tttatacca gatgatatg acttttgat gacgaacct 
      aaagaacaa tgcgacaaa ctacccgaa ctgaataaa 
      taccagccg agatttttc acaaccaca gccttgcaa 
      caagccaag gttgatcta acttgggac gaaaccgat 
      ccagataga atcgaaatg acccaaaag ctcaacact 
      ggcaaaatt gaagaaata ccagaacat gagcttcag 
      aatttcgtc gcagtgagt gatgaggag agcgaagat 
      gaggagaaa aacgccact tcagacgat gacgaaagc 
      actaataac ccaatcgat aaatacaaa caactcctg 
      caagaaatc gaagagaaa gaacaggag aagaaaaac 
      agagaagtg gaaatggaa gtgagctgg ggcatcgat 
      ttgaaggac aaaaccgaa aagttggta aagaagaag 
      attgaaaat gaggaagaa aagacacca ttccagaaa 
      tatctcgat aaacggaaa gaaaaacgc aaagagaag 
      aaaaagaag aagcgggaa aatgaagat tcggatgat 
      gtaccgtct gatattgat atgaacgat ccgtatttc 
      gccgaggag tttaacaat cccgaattt aagaaaaag 
      aaagttaag gagaagaaa tctcagtcg gaagaagaa 
      aataatcct gaattggag ttacttttg gctgatgaa 
      gatgacgat aataagaag cacttcagt ttgaagaaa 
      attcagaag caagaagaa gcgactagt aagaaaaaa 
      cgcaaactc aaagaaaca gacgagaag gacgatttt 
      caagtcgat gttaatgat gatcggttc tctgccctc 
      tttacgagt catttgtat aatattgat cccacggac 
      ccccattat aagaagacg aaaggtatg gagagacta 
      ataaatgag aaaatcaaa aggcggaaa gacgatagc 
      gaacgtgaa atgacggag aagccgatg aaaaaagat 
      gccgagttg agtgttttg gtaaagtca gtgaaacgg 
      aaagctgaa gcgttgagt aataagaaa aagtga
    mRNA sequence
    • >TC007375 RA
      tacgaaata acctgtcaa aaacacgtg tgttgttgt 
      gaatgtggg gttaaaata tttttttta ttaaaataa 
      actgaagca atggaagat aaacgcttc gcgcacata 
      aaaaccgac cccaaattc cggcggatc cccaaaggg 
      gcccgcaag gttaaaatc gacaagcgg ttccagtcc 
      atgttcaaa gacaagaag ttcaaagtg aaatacacg 
      gtggacaag cgtgggaga cccgtaaat cacacatcg 
      actgaagat ctgaagcgt tattacgaa ctgagttcg 
      agtgagagt tcctcagat gaagaacca gaagagccc 
      ggcaagtac gtcaaagcg ccccaaaac gacctaaat 
      gtgaaagat aaaagcgat aaggagctt cccagcgac 
      gtgaagaag aaacttcgt gacttgaat gtcgactac 
      gcccgaggg gagggaaat ttattgagt gagagttct 
      tcagatgac gaggaaacg gatgaggaa gtgtctgaa 
      ggggagcag attgagcac aaatgggga gagttggat 
      aatgacgcc gataggaca gatgaagcc acgtatcgt 
      ttggcggct tgtaacctg gactgggat aggataagg 
      gcggtagat ttgatggtc ctgttcaat tcgtttttg 
      ccccccggt ggggctgtt aagtcagtt gtgatttat 
      ccctcggag tttggcaaa gaacgaatg aaggaagag 
      gaggtgaaa gggccaatt gaattggtg gaacacaag 
      gaaattgag gaagaagac acggaagaa ggctcaaaa 
      tatcacatg gaaaaattg aggcagtac caactcaac 
      cgactgaag tattattac gctgttgtg acctttgac 
      ggggctaac actgcaaat aaagtgtac acagagtgt 
      gacgggatg gagtatgaa tcaagcgcg acaaagctt 
      gatttacgc tttatacca gatgatatg acttttgat 
      gacgaacct aaagaacaa tgcgacaaa ctacccgaa 
      ctgaataaa taccagccg agatttttc acaaccaca 
      gccttgcaa caagccaag gttgatcta acttgggac 
      gaaaccgat ccagataga atcgaaatg acccaaaag 
      ctcaacact ggcaaaatt gaagaaata ccagaacat 
      gagcttcag aatttcgtc gcagtgagt gatgaggag 
      agcgaagat gaggagaaa aacgccact tcagacgat 
      gacgaaagc actaataac ccaatcgat aaatacaaa 
      caactcctg caagaaatc gaagagaaa gaacaggag 
      aagaaaaac agagaagtg gaaatggaa gtgagctgg 
      ggcatcgat ttgaaggac aaaaccgaa aagttggta 
      aagaagaag attgaaaat gaggaagaa aagacacca 
      ttccagaaa tatctcgat aaacggaaa gaaaaacgc 
      aaagagaag aaaaagaag aagcgggaa aatgaagat 
      tcggatgat gtaccgtct gatattgat atgaacgat 
      ccgtatttc gccgaggag tttaacaat cccgaattt 
      aagaaaaag aaagttaag gagaagaaa tctcagtcg 
      gaagaagaa aataatcct gaattggag ttacttttg 
      gctgatgaa gatgacgat aataagaag cacttcagt 
      ttgaagaaa attcagaag caagaagaa gcgactagt 
      aagaaaaaa cgcaaactc aaagaaaca gacgagaag 
      gacgatttt caagtcgat gttaatgat gatcggttc 
      tctgccctc tttacgagt catttgtat aatattgat 
      cccacggac ccccattat aagaagacg aaaggtatg 
      gagagacta ataaatgag aaaatcaaa aggcggaaa 
      gacgatagc gaacgtgaa atgacggag aagccgatg 
      aaaaaagat gccgagttg agtgttttg gtaaagtca 
      gtgaaacgg aaagctgaa gcgttgagt aataagaaa 
      aagtgactt gtaagcaaa tgtcttttt tatgtaaat 
      aaacggtcc ttataaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY