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Gene information
General information
TC008096 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4992813..4994381
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008096
    iBeetle screen iB_09895

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008096 RA
      MNRPYIRII EQPQKSLRF RYQCEKRTG NIPGASSTS 
      TLKTVCAIQ IVGYQGRAA VVVSCVTKD EPHKPHPHK 
      LVGEHCKKG VYTFEAEIT PNNPDIVFR KLGVQCVKR 
      NELKESLNV REEKNIDPF KTGFSHKDH LNCIDLNVV 
      RLCFQVFLI DENGRFTSG LAPVVSEPI YNRKTFPDP 
      DIFRLSHCN ASVDGGKND IILLCPKVD KDDIRIRIY 
      ETANGQTLW QYFINGDQT QIYSSTAIC FKPPPYKNL 
      NIVEPVPVF IQLVRPSDE VVSKSVQFE YLPLDAEPG 
      FLKEKRKKC QDMLPLFAQ LLDNPGPSH AMSLENLPN 
      FPSGDINIE SASGVSKLL NIDCHQIEL EPINISSGD 
      LEMFDVNRL SGSLQENLS LSDVLV
    CDS sequence
    • >TC008096 RA
      atgaaccgc ccctacatc cgcatcatc gagcaaccg 
      caaaaatcg ctccggttc cggtaccag tgcgagaag 
      cgcaccgga aacatcccc ggagcttcc agcacttca 
      acccttaaa acagtgtgc gccatccaa atcgtgggg 
      taccagggc cgggccgct gtggttgtc tcctgtgtg 
      actaaagac gagccccac aagccacac ccccataaa 
      ctcgtggga gagcactgc aaaaaggga gtttacacc 
      ttcgaagcg gaaatcacc ccgaacaac cctgatatc 
      gtcttccgg aagttgggg gtgcagtgc gtgaaaagg 
      aacgaactc aaggagagt ctcaatgtg cgagaagaa 
      aaaaatatt gatccgttt aaaaccggg ttttcgcac 
      aaagaccat ttgaattgc atcgacttg aatgtggtc 
      cggttgtgt ttccaagtg tttctaatc gacgaaaat 
      ggccggttt acctccggg ttagctccg gttgtgagt 
      gagccgatt tacaaccgg aagactttc ccggatccg 
      gatatcttc cggttgtct cattgcaac gccagtgtg 
      gacggcgga aaaaacgat attatcctg ttatgtccc 
      aaggtcgat aaagacgac atccgaata cgaatttac 
      gaaacggca aatggtcag actttatgg cagtacttt 
      ataaacggc gaccaaacg caaatctac agcagcaca 
      gccatttgt ttcaaaccc ccgccgtac aaaaactta 
      aatattgtc gagcccgtc cctgtcttc attcaacta 
      gttcggccc tctgacgag gtcgtcagt aaatcagtc 
      cagtttgaa tatttaccc ctagatgca gagccgggg 
      tttttgaaa gaaaaacgg aaaaaatgc caggatatg 
      ttaccactt tttgcacaa cttttggac aatcctggc 
      ccaagtcac gcaatgtct ctagaaaac ttaccgaat 
      tttccaagt ggagacatt aatattgaa agtgcgagc 
      ggtgtaagc aaactactg aatatcgat tgccaccaa 
      atcgaactc gaaccgatt aatatcagt tcaggtgac 
      ttggaaatg ttcgatgta aatagattg tcgggcagt 
      ttgcaagag aatttgtca ctgagtgat gtactagtt 
      taa
    mRNA sequence
    • >TC008096 RA
      tcagtcaaa atcacgcga cgcccagtt accgattcc 
      cgataaata aaaaaaatg ctacaactg gttataaat 
      tctcatgaa accacaaag catttcatt tattataaa 
      ctaagaaag gtcgcgtag gttcgcagt cgcatcaac 
      tcttgcaaa ccttgaaac acatccaga taatgcgta 
      tgagtttcc accgtttcg catcaccta ggtaaacag 
      caaaatctc attcattta aattttttc tcgaagcaa 
      tgaaccgcc cctacatcc gcatcatcg agcaaccgc 
      aaaaatcgc tccggttcc ggtaccagt gcgagaagc 
      gcaccggaa acatccccg gagcttcca gcacttcaa 
      cccttaaaa cagtgtgcg ccatccaaa tcgtggggt 
      accagggcc gggccgctg tggttgtct cctgtgtga 
      ctaaagacg agccccaca agccacacc cccataaac 
      tcgtgggag agcactgca aaaagggag tttacacct 
      tcgaagcgg aaatcaccc cgaacaacc ctgatatcg 
      tcttccgga agttggggg tgcagtgcg tgaaaagga 
      acgaactca aggagagtc tcaatgtgc gagaagaaa 
      aaaatattg atccgttta aaaccgggt tttcgcaca 
      aagaccatt tgaattgca tcgacttga atgtggtcc 
      ggttgtgtt tccaagtgt ttctaatcg acgaaaatg 
      gccggttta cctccgggt tagctccgg ttgtgagtg 
      agccgattt acaaccgga agactttcc cggatccgg 
      atatcttcc ggttgtctc attgcaacg ccagtgtgg 
      acggcggaa aaaacgata ttatcctgt tatgtccca 
      aggtcgata aagacgaca tccgaatac gaatttacg 
      aaacggcaa atggtcaga ctttatggc agtacttta 
      taaacggcg accaaacgc aaatctaca gcagcacag 
      ccatttgtt tcaaacccc cgccgtaca aaaacttaa 
      atattgtcg agcccgtcc ctgtcttca ttcaactag 
      ttcggccct ctgacgagg tcgtcagta aatcagtcc 
      agtttgaat atttacccc tagatgcag agccggggt 
      ttttgaaag aaaaacgga aaaaatgcc aggatatgt 
      taccacttt ttgcacaac ttttggaca atcctggcc 
      caagtcacg caatgtctc tagaaaact taccgaatt 
      ttccaagtg gagacatta atattgaaa gtgcgagcg 
      gtgtaagca aactactga atatcgatt gccaccaaa 
      tcgaactcg aaccgatta atatcagtt caggtgact 
      tggaaatgt tcgatgtaa atagattgt cgggcagtt 
      tgcaagaga atttgtcac tgagtgatg tactagttt 
      aaggaaata aatttctac aaaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY